Prednisona e meloxicam no tratamento de ratos submetidos ao trauma agudo da medula espinhal
Ano de defesa: | 2012 |
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Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Santa Maria
|
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Medicina Veterinária
|
Departamento: |
Medicina Veterinária
|
País: |
BR
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.ufsm.br/handle/1/10126 |
Resumo: | Considering the relevance of study of traumatic spinal cord injury in humans and animals, the aim of the study was investigated the use of the prednisone and meloxicam in treatment of rats submitted the experimental model of acute spinal cord injury with 2Fr Fogarty catheter with evaluation of oxidative stress, Neurological tests and histological exam. Ninety rats were divided in six groups: GCS or saline (n=15), GCP or prednisone control (n=15), GCM or meloxicam control, GTS or trauma saline (n=15), GTP or trauma meloxicam (n=15) and GTM or trauma prednisone (n=15). Each group was divided into three subgroups according to treatment time in the postoperative period of 24h, 72h and seven days. All groups were submitted laminectomy and in the groups GTS, GTM and GTP, after exposure of the spinal cord was performed compressive spinal cord injury with 2Fr Fogarty catheter. The GCS and GTS was treated with saline (0,5ml/rat/day), GSM and GTM with meloxicam (2mg/kg/day) and GSP and GTP with prednisone (2mg/kg/dia). Neurological tests were performed (BBB, inclined plane and deep pain) 24 hours before and after surgery and repeated every 48 hours until the day of euthanasia. The parameters of oxidative stress were evaluated and performed histopathological analysis of the spinal cord. The groups GTS, GTM and GTP in the different times were no point in the BBB scale and three points in the inclined plane and absence of deep pain. GTM and GTS groups had lower catalase activity and TBARS levels when compared to the GTS and they were more evident to 72 hours postoperative period, indicating a possible antioxidant effect. In the GTS group, there was persistence of this action until the seventh day after trauma. In the histopathological exam were found Wallerian degeneration and necrosis of gray matter of intensity variation, with no significant difference between undergone to trauma. Meloxicam and prednisone can have neuroprotective and antioxidant effect, but the necrosis and Wallerian degeneration were not stop in rats submitted the acute spinal cord injury with 2Fr Fogarty catheter. |
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2017-06-072017-06-072012-02-29AIELLO, Graciane. Prednisone and meloxicam in the treatment of rats submitted to compressive spinal cord injury. 2012. 62 f. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2012.http://repositorio.ufsm.br/handle/1/10126Considering the relevance of study of traumatic spinal cord injury in humans and animals, the aim of the study was investigated the use of the prednisone and meloxicam in treatment of rats submitted the experimental model of acute spinal cord injury with 2Fr Fogarty catheter with evaluation of oxidative stress, Neurological tests and histological exam. Ninety rats were divided in six groups: GCS or saline (n=15), GCP or prednisone control (n=15), GCM or meloxicam control, GTS or trauma saline (n=15), GTP or trauma meloxicam (n=15) and GTM or trauma prednisone (n=15). Each group was divided into three subgroups according to treatment time in the postoperative period of 24h, 72h and seven days. All groups were submitted laminectomy and in the groups GTS, GTM and GTP, after exposure of the spinal cord was performed compressive spinal cord injury with 2Fr Fogarty catheter. The GCS and GTS was treated with saline (0,5ml/rat/day), GSM and GTM with meloxicam (2mg/kg/day) and GSP and GTP with prednisone (2mg/kg/dia). Neurological tests were performed (BBB, inclined plane and deep pain) 24 hours before and after surgery and repeated every 48 hours until the day of euthanasia. The parameters of oxidative stress were evaluated and performed histopathological analysis of the spinal cord. The groups GTS, GTM and GTP in the different times were no point in the BBB scale and three points in the inclined plane and absence of deep pain. GTM and GTS groups had lower catalase activity and TBARS levels when compared to the GTS and they were more evident to 72 hours postoperative period, indicating a possible antioxidant effect. In the GTS group, there was persistence of this action until the seventh day after trauma. In the histopathological exam were found Wallerian degeneration and necrosis of gray matter of intensity variation, with no significant difference between undergone to trauma. Meloxicam and prednisone can have neuroprotective and antioxidant effect, but the necrosis and Wallerian degeneration were not stop in rats submitted the acute spinal cord injury with 2Fr Fogarty catheter.Considerando a relevância dos estudos destinados às lesões traumáticas da medula espinhal em humanos e animais, o objetivo deste estudo foi investigar o efeito da prednisona e meloxicam na terapia de ratos submetidos ao modelo experimental de trauma agudo da medula espinhal induzida pelo cateter de Fogarty 2Fr, mediante avaliação do perfil oxidativo, dos testes neurológicos e exame histopatológico da medula espinhal. Foram utilizados 90 ratos Wistar, distribuídos em seis grupos denominados GCS ou salina (n=15), GCP ou controle prednisona (n=15), GCM ou controle meloxicam (n=15), GTS ou trauma mais salina (n=15), GTP ou trauma mais prednisona (n=15) e GTM ou trauma mais meloxicam (n=15). Cada grupo foi redistribuído em três subgrupos de acordo com o tempo de tratamento no pós-operatório de 24h, 72h e sete dias. Todos os grupos foram submetidos à laminectomia e nos grupos GTS, GTM e GTP, após a exposição da medula espinhal, foi realizado o trauma medular compressivo utilizando o cateter Fogarty 2Fr. Os grupos GCS e GTS foram tratados com solução salina (0,5ml/rato/dia), os GSM e GTM receberam meloxicam (2mg/kg/dia) e os grupos GSP e GTP receberam prednisona (2mg/kg/dia). Foram realizados testes neurológicos (BBB, plano inclinado e percepção a dor profunda) 24 horas antes e após o procedimento cirúrgico e repetido a cada 48 horas até a realização da eutanásia, ou seja, 24h, 72h e sete dias. Foram avaliados os parâmetros do estresse oxidativo através da enzima catalase, quantificado as substâncias reativas ao ácido tiobarbitúrico (TBARS) e realizado exame histopatológico da medula espinhal. Os ratos dos grupos GTS, GTM e GTP e nos diferentes tempos (24h, 72h e sete dias) tiveram pontuação zero na escala BBB, ou seja, nenhum movimento observado no membro pélvico; no plano inclinado, permaneceram com pontuação 3, ou seja, em um ângulo dez graus menor que antes da cirurgia e perda da percepção da dor profunda Os grupos GTM e GTS apresentaram menor atividade da catalase e níveis de TBARS quando comparado ao grupo GTS e foi mais evidente nas primeiras 72 horas de PO, indicando um possível efeito antioxidante. No grupo GTS, houve persistência desta ação até o sétimo dia pós-trauma. No exame histopatológico da medula espinhal foi constatada degeneração Waleriana e necrose da substância cinzenta de intensidades variáveis, não apresentando diferença entre os grupos submetidos ao trauma. Conclui-se que o meloxicam e a prednisona apresentam possível efeito antioxidante e neuroprotetora, mas não impedem a necrose e a degeneração Walleriana da medula espinhal de ratos submetidos a trauma medular agudo utilizando o cateter de Fogarty 2Fr.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em Medicina VeterináriaUFSMBRMedicina VeterináriaPeroxidação lipídicaCatalaseCateter de Fogarty 2FrLipid peroxidationCatalase2Fr Fogarty catheterCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIAPrednisona e meloxicam no tratamento de ratos submetidos ao trauma agudo da medula espinhalPrednisone and meloxicam in the treatment of rats submitted to compressive spinal cord injuryinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisMazzanti, Alexandrehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4795593E6Melo, Eliane Gonçalves dehttp://lattes.cnpq.br/5711934186393745Mazzanti, Cinthia Melazzo Andradehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4776635P4http://lattes.cnpq.br/2350002573405660Aiello, Graciane500500000007400500500300500425ee272-e777-4ddd-a2ec-1bdf5b0a21ec5b2fcc36-66a4-4713-8c99-d61a2767719f954c9957-bae8-465f-9082-c70be251ea6706219c3e-9788-4345-8060-bf224f77f2e3info:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALAIELLO, GRACIANE.pdfapplication/pdf811677http://repositorio.ufsm.br/bitstream/1/10126/1/AIELLO%2c%20GRACIANE.pdfdd819cbe2a4c00ca2b9cff32ade4ed98MD51TEXTAIELLO, GRACIANE.pdf.txtAIELLO, GRACIANE.pdf.txtExtracted texttext/plain116548http://repositorio.ufsm.br/bitstream/1/10126/2/AIELLO%2c%20GRACIANE.pdf.txte020782f2b59a1d5b7d7370b09c15058MD52THUMBNAILAIELLO, GRACIANE.pdf.jpgAIELLO, GRACIANE.pdf.jpgIM Thumbnailimage/jpeg5334http://repositorio.ufsm.br/bitstream/1/10126/3/AIELLO%2c%20GRACIANE.pdf.jpgdb2b641fc5f69fb4cfed73f787ccea13MD531/101262017-07-25 12:01:43.093oai:repositorio.ufsm.br:1/10126Repositório Institucionalhttp://repositorio.ufsm.br/PUBhttp://repositorio.ufsm.br/oai/requestopendoar:39132017-07-25T15:01:43Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Prednisona e meloxicam no tratamento de ratos submetidos ao trauma agudo da medula espinhal |
dc.title.alternative.eng.fl_str_mv |
Prednisone and meloxicam in the treatment of rats submitted to compressive spinal cord injury |
title |
Prednisona e meloxicam no tratamento de ratos submetidos ao trauma agudo da medula espinhal |
spellingShingle |
Prednisona e meloxicam no tratamento de ratos submetidos ao trauma agudo da medula espinhal Aiello, Graciane Peroxidação lipídica Catalase Cateter de Fogarty 2Fr Lipid peroxidation Catalase 2Fr Fogarty catheter CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Prednisona e meloxicam no tratamento de ratos submetidos ao trauma agudo da medula espinhal |
title_full |
Prednisona e meloxicam no tratamento de ratos submetidos ao trauma agudo da medula espinhal |
title_fullStr |
Prednisona e meloxicam no tratamento de ratos submetidos ao trauma agudo da medula espinhal |
title_full_unstemmed |
Prednisona e meloxicam no tratamento de ratos submetidos ao trauma agudo da medula espinhal |
title_sort |
Prednisona e meloxicam no tratamento de ratos submetidos ao trauma agudo da medula espinhal |
author |
Aiello, Graciane |
author_facet |
Aiello, Graciane |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Mazzanti, Alexandre |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4795593E6 |
dc.contributor.referee1.fl_str_mv |
Melo, Eliane Gonçalves de |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/5711934186393745 |
dc.contributor.referee2.fl_str_mv |
Mazzanti, Cinthia Melazzo Andrade |
dc.contributor.referee2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4776635P4 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/2350002573405660 |
dc.contributor.author.fl_str_mv |
Aiello, Graciane |
contributor_str_mv |
Mazzanti, Alexandre Melo, Eliane Gonçalves de Mazzanti, Cinthia Melazzo Andrade |
dc.subject.por.fl_str_mv |
Peroxidação lipídica Catalase Cateter de Fogarty 2Fr |
topic |
Peroxidação lipídica Catalase Cateter de Fogarty 2Fr Lipid peroxidation Catalase 2Fr Fogarty catheter CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
dc.subject.eng.fl_str_mv |
Lipid peroxidation Catalase 2Fr Fogarty catheter |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
Considering the relevance of study of traumatic spinal cord injury in humans and animals, the aim of the study was investigated the use of the prednisone and meloxicam in treatment of rats submitted the experimental model of acute spinal cord injury with 2Fr Fogarty catheter with evaluation of oxidative stress, Neurological tests and histological exam. Ninety rats were divided in six groups: GCS or saline (n=15), GCP or prednisone control (n=15), GCM or meloxicam control, GTS or trauma saline (n=15), GTP or trauma meloxicam (n=15) and GTM or trauma prednisone (n=15). Each group was divided into three subgroups according to treatment time in the postoperative period of 24h, 72h and seven days. All groups were submitted laminectomy and in the groups GTS, GTM and GTP, after exposure of the spinal cord was performed compressive spinal cord injury with 2Fr Fogarty catheter. The GCS and GTS was treated with saline (0,5ml/rat/day), GSM and GTM with meloxicam (2mg/kg/day) and GSP and GTP with prednisone (2mg/kg/dia). Neurological tests were performed (BBB, inclined plane and deep pain) 24 hours before and after surgery and repeated every 48 hours until the day of euthanasia. The parameters of oxidative stress were evaluated and performed histopathological analysis of the spinal cord. The groups GTS, GTM and GTP in the different times were no point in the BBB scale and three points in the inclined plane and absence of deep pain. GTM and GTS groups had lower catalase activity and TBARS levels when compared to the GTS and they were more evident to 72 hours postoperative period, indicating a possible antioxidant effect. In the GTS group, there was persistence of this action until the seventh day after trauma. In the histopathological exam were found Wallerian degeneration and necrosis of gray matter of intensity variation, with no significant difference between undergone to trauma. Meloxicam and prednisone can have neuroprotective and antioxidant effect, but the necrosis and Wallerian degeneration were not stop in rats submitted the acute spinal cord injury with 2Fr Fogarty catheter. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-02-29 |
dc.date.accessioned.fl_str_mv |
2017-06-07 |
dc.date.available.fl_str_mv |
2017-06-07 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
AIELLO, Graciane. Prednisone and meloxicam in the treatment of rats submitted to compressive spinal cord injury. 2012. 62 f. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2012. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/10126 |
identifier_str_mv |
AIELLO, Graciane. Prednisone and meloxicam in the treatment of rats submitted to compressive spinal cord injury. 2012. 62 f. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2012. |
url |
http://repositorio.ufsm.br/handle/1/10126 |
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por |
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500500000007 |
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400 500 500 300 500 |
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Universidade Federal de Santa Maria |
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Programa de Pós-Graduação em Medicina Veterinária |
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UFSM |
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BR |
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Medicina Veterinária |
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Universidade Federal de Santa Maria |
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