Participação do receptor TRPA1 na nocicepção observada em um modelo de esclerose múltipla progressiva em camundongos
Ano de defesa: | 2020 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Santa Maria
Centro de Ciências da Saúde |
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Farmacologia
|
Departamento: |
Farmacologia
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.ufsm.br/handle/1/24007 |
Resumo: | Central neuropathic pain (CNP) is a common untreated symptom in progressive multiple sclerosis (PMS) and is associated with poor quality of life and interference with patients’ daily activities. The neuroinflammation process and mitochondrial dysfunction in the PPMS lesions generate reactive species. The transient potential receptor ankyrin 1 (TRPA1) has been identified as one of the major mechanisms that contribute to neuropathic pain signaling and can be activated by products of oxidative stress. Thus, the goal of our study was to evaluate the role of spinal cord TRPA1 in a PPMS mice model. C57BL/6 female mice (20-30g) were used and the PMS model was induced by the experimental autoimmune encephalomyelitis (EAE) using MOG35-55 antigen and CFA (complete Freund’s adjuvant). Mice developed progressive clinical score, with motor impairment observed after 15 days of induction. This model induced progressive mechanical and cold allodynia, and heat hyperalgesia which were measured up to 14 days after induction. The hypersensitivity observed was reduced by the administration of selective TRPA1 antagonists (HC-030031 and A967079, via intrathecal and intragastric), antioxidants (α-lipoic acid and apocynin, via intrathecal and intragastric), and TRPA1 antisense oligonucleotide (via intrathecal). We also observed an increase in TRPA1 mRNA levels, NADPH oxidase activity, and 4-hydroxinonenal (a TRPA1 agonist) levels in spinal cord sample of PMS-induced animals. In conclusion, these results support the hypothesis of the TRPA1 receptor involvement in nociception observed in a PMS model in mice. |
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2022-04-04T13:28:05Z2022-04-04T13:28:05Z2020-03-25http://repositorio.ufsm.br/handle/1/24007Central neuropathic pain (CNP) is a common untreated symptom in progressive multiple sclerosis (PMS) and is associated with poor quality of life and interference with patients’ daily activities. The neuroinflammation process and mitochondrial dysfunction in the PPMS lesions generate reactive species. The transient potential receptor ankyrin 1 (TRPA1) has been identified as one of the major mechanisms that contribute to neuropathic pain signaling and can be activated by products of oxidative stress. Thus, the goal of our study was to evaluate the role of spinal cord TRPA1 in a PPMS mice model. C57BL/6 female mice (20-30g) were used and the PMS model was induced by the experimental autoimmune encephalomyelitis (EAE) using MOG35-55 antigen and CFA (complete Freund’s adjuvant). Mice developed progressive clinical score, with motor impairment observed after 15 days of induction. This model induced progressive mechanical and cold allodynia, and heat hyperalgesia which were measured up to 14 days after induction. The hypersensitivity observed was reduced by the administration of selective TRPA1 antagonists (HC-030031 and A967079, via intrathecal and intragastric), antioxidants (α-lipoic acid and apocynin, via intrathecal and intragastric), and TRPA1 antisense oligonucleotide (via intrathecal). We also observed an increase in TRPA1 mRNA levels, NADPH oxidase activity, and 4-hydroxinonenal (a TRPA1 agonist) levels in spinal cord sample of PMS-induced animals. In conclusion, these results support the hypothesis of the TRPA1 receptor involvement in nociception observed in a PMS model in mice.A dor neuropática central (DNC) é um sintoma comum na esclerose múltipla progressiva (EMP) e e por ainda ser de difícil tratamento está associada a baixa qualidade de vida dos pacientes. O processo da neuroinflamação e disfunção mitocondrial nas lesões de EMP geram espécies reativas. O receptor de potencial transitório anquirina 1 (TRPA1) é identificado como um dos principais mecanismos que contribuem para a sinalização da dor neuropática e pode ser ativado por produtos do estresse oxidativo. Assim, o objetivo deste estudo foi avaliar o papel do receptor TRPA1 na medula espinhal em um modelo de EMP em camundongos. Camundongos fêmeas C57BL/6 (20-30g) foram utilizados e o modelo EMP foi induzido pela encefalomielite autoimune experimental (EAE) usando o antígeno MOG35-55 e ACF (adjuvante completo de Freund). Os camundongos desenvolveram escore clínico progressivo, com comprometimento motor observado após 15 dias de indução. Este modelo induziu alodínia mecânica e ao frio e hiperalgesia ao calor que foram medidas até 14 dias após a indução. A hipersensibilidade observada foi reduzida pela administração de antagonistas seletivos do TRPA1 (HC-030031 e A967079, via intratecal e intragástrica), antioxidantes (ácido α-lipóico e apocinina, via intratecal e intragástrica) e oligonucleotídeo antisentido do TRPA1 (via intratecal). Também foi observado um aumento nos níveis de mRNA do TRPA1, atividade de NADPH oxidase e níveis de 4-hidroxinonenal (um agonista de TRPA1) em amostra da medula espinal de animais induzidos por EMP. Em conclusão, esses resultados corroboram a hipótese do envolvimento do receptor TRPA1 na nocicepção observada em um modelo de EMP em camundongos.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESporUniversidade Federal de Santa MariaCentro de Ciências da SaúdePrograma de Pós-Graduação em FarmacologiaUFSMBrasilFarmacologiaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessDor neuropática central4-HNENADPH oxidaseEAEApocininaÁcido α-lipóicoCentral neuropathic painApocyninα-lipoic acidCNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIAParticipação do receptor TRPA1 na nocicepção observada em um modelo de esclerose múltipla progressiva em camundongosParticipation of the TRPA1 receptor in the nociception observed in a progressive multiple sclerosis model in miceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisSantos, Gabriela Trevisan doshttp://lattes.cnpq.br/7186082133291911André, EuniceOliveira, Mauro Schneiderhttp://lattes.cnpq.br/8009327577037237Ritter, Camila dos Santos201000000000600600600600600a3d4449a-dee6-4203-816c-7784a9fd9125d37f758c-5e84-4b6f-a9d3-3daa5bcbffe370ef9984-c273-40ec-9321-b79fde32965ec4b00ac8-dd1a-4fc3-86e1-a4275c044653reponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALDIS_PPGFARMACOLOGIA_2020_RITTER_CAMILA.pdfDIS_PPGFARMACOLOGIA_2020_RITTER_CAMILA.pdfDissertação de Mestradoapplication/pdf2550649http://repositorio.ufsm.br/bitstream/1/24007/1/DIS_PPGFARMACOLOGIA_2020_RITTER_CAMILA.pdf740e649bae47bd6216edd94a54be2119MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8805http://repositorio.ufsm.br/bitstream/1/24007/2/license_rdf4460e5956bc1d1639be9ae6146a50347MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81956http://repositorio.ufsm.br/bitstream/1/24007/3/license.txt2f0571ecee68693bd5cd3f17c1e075dfMD531/240072022-04-04 10:28:05.641oai:repositorio.ufsm.br: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ório Institucionalhttp://repositorio.ufsm.br/PUBhttp://repositorio.ufsm.br/oai/requestopendoar:39132022-04-04T13:28:05Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Participação do receptor TRPA1 na nocicepção observada em um modelo de esclerose múltipla progressiva em camundongos |
dc.title.alternative.eng.fl_str_mv |
Participation of the TRPA1 receptor in the nociception observed in a progressive multiple sclerosis model in mice |
title |
Participação do receptor TRPA1 na nocicepção observada em um modelo de esclerose múltipla progressiva em camundongos |
spellingShingle |
Participação do receptor TRPA1 na nocicepção observada em um modelo de esclerose múltipla progressiva em camundongos Ritter, Camila dos Santos Dor neuropática central 4-HNE NADPH oxidase EAE Apocinina Ácido α-lipóico Central neuropathic pain Apocynin α-lipoic acid CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA |
title_short |
Participação do receptor TRPA1 na nocicepção observada em um modelo de esclerose múltipla progressiva em camundongos |
title_full |
Participação do receptor TRPA1 na nocicepção observada em um modelo de esclerose múltipla progressiva em camundongos |
title_fullStr |
Participação do receptor TRPA1 na nocicepção observada em um modelo de esclerose múltipla progressiva em camundongos |
title_full_unstemmed |
Participação do receptor TRPA1 na nocicepção observada em um modelo de esclerose múltipla progressiva em camundongos |
title_sort |
Participação do receptor TRPA1 na nocicepção observada em um modelo de esclerose múltipla progressiva em camundongos |
author |
Ritter, Camila dos Santos |
author_facet |
Ritter, Camila dos Santos |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Santos, Gabriela Trevisan dos |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/7186082133291911 |
dc.contributor.referee1.fl_str_mv |
André, Eunice |
dc.contributor.referee2.fl_str_mv |
Oliveira, Mauro Schneider |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/8009327577037237 |
dc.contributor.author.fl_str_mv |
Ritter, Camila dos Santos |
contributor_str_mv |
Santos, Gabriela Trevisan dos André, Eunice Oliveira, Mauro Schneider |
dc.subject.por.fl_str_mv |
Dor neuropática central 4-HNE NADPH oxidase EAE Apocinina Ácido α-lipóico |
topic |
Dor neuropática central 4-HNE NADPH oxidase EAE Apocinina Ácido α-lipóico Central neuropathic pain Apocynin α-lipoic acid CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA |
dc.subject.eng.fl_str_mv |
Central neuropathic pain Apocynin α-lipoic acid |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA |
description |
Central neuropathic pain (CNP) is a common untreated symptom in progressive multiple sclerosis (PMS) and is associated with poor quality of life and interference with patients’ daily activities. The neuroinflammation process and mitochondrial dysfunction in the PPMS lesions generate reactive species. The transient potential receptor ankyrin 1 (TRPA1) has been identified as one of the major mechanisms that contribute to neuropathic pain signaling and can be activated by products of oxidative stress. Thus, the goal of our study was to evaluate the role of spinal cord TRPA1 in a PPMS mice model. C57BL/6 female mice (20-30g) were used and the PMS model was induced by the experimental autoimmune encephalomyelitis (EAE) using MOG35-55 antigen and CFA (complete Freund’s adjuvant). Mice developed progressive clinical score, with motor impairment observed after 15 days of induction. This model induced progressive mechanical and cold allodynia, and heat hyperalgesia which were measured up to 14 days after induction. The hypersensitivity observed was reduced by the administration of selective TRPA1 antagonists (HC-030031 and A967079, via intrathecal and intragastric), antioxidants (α-lipoic acid and apocynin, via intrathecal and intragastric), and TRPA1 antisense oligonucleotide (via intrathecal). We also observed an increase in TRPA1 mRNA levels, NADPH oxidase activity, and 4-hydroxinonenal (a TRPA1 agonist) levels in spinal cord sample of PMS-induced animals. In conclusion, these results support the hypothesis of the TRPA1 receptor involvement in nociception observed in a PMS model in mice. |
publishDate |
2020 |
dc.date.issued.fl_str_mv |
2020-03-25 |
dc.date.accessioned.fl_str_mv |
2022-04-04T13:28:05Z |
dc.date.available.fl_str_mv |
2022-04-04T13:28:05Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
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publishedVersion |
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http://repositorio.ufsm.br/handle/1/24007 |
url |
http://repositorio.ufsm.br/handle/1/24007 |
dc.language.iso.fl_str_mv |
por |
language |
por |
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201000000000 |
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600 600 600 600 600 |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
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openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências da Saúde |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Farmacologia |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Farmacologia |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências da Saúde |
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