Avaliação de metaloestrógenos em cabelo de mulheres com câncer de mama por LA-ICP-MS
Ano de defesa: | 2021 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Santa Maria
Centro de Ciências Naturais e Exatas |
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Química
|
Departamento: |
Química
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.ufsm.br/handle/1/23066 |
Resumo: | In this work, the relationship of metalloestrogens (Al, Sb, As, Ba, Cd, Cr, Co, Cu, Pb, Hg, Ni, Se, Sn, V, and Zn) with breast cancer in women with age around 59 years. Laser ablation coupled to inductively coupled plasma mass spectrometry (LA-ICP-MS) was used to evaluate the changes of these elements along a hair strand while ICP-MS and inductively coupled plasma optical emission (ICP OES) was used to determine elements concentration in hair after sample decomposition. Scanning electron microscopy (SEM) and LA-ICP-MS were used to evaluate the effect on hair washing. Hair washing was done according to the procedure recommended by the International Atomic Energy Agency (IEAE). Hair strands were fixed on a glass slice with the aid of double-side tape and then analyzed. Hair samples without washing were analyzed in the same way. Changes in hair morphology were observed by the analyses with SEM after hair washing, while no changes in element concentration were observed when hair strands were analyzed by LA-ICP-MS after washing. For hair analysis by LA-ICP-MS, laser ablation conditions were fixed to 50 μm, 80 μm s-1 and 26.7 J cm-2, for the spot size, laser speed and ablation energy, respectively. Operational conditions of the ICP-MS instrument were adjusted in order to achieve the best sensitivity. Calibration in LA-ICP-MS was performed by using an internal standard (sulphur in the form of l-cysteine) and filter paper disc (17 mm diameter) as support of the analytes and internal standard. The way to add standard and internal standard solution to the filter paper was evaluate where the best results were achieved by the addition a mixture of the standard solution plus internal standard solution. For ICP-MS and ICP OES analyses, samples were decomposed with nitric acid and heating with the aid of microwave energy. From the analyzes by ICP-MS, ICP OES, and LA-ICP-MS it was observed that the concentrations of the elements in the samples varied, with Ba being the element present in the highest concentration in the samples. By analyzing the results obtained by ICP-MS and ICP OES with statistic tools, it was possible to observe higher concentrations of Ni, Cu, Cd and Sb in hair of women with cancer than women without cancer (Control individuals). |
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2021-11-30T12:56:49Z2021-11-30T12:56:49Z2021-05-07http://repositorio.ufsm.br/handle/1/23066In this work, the relationship of metalloestrogens (Al, Sb, As, Ba, Cd, Cr, Co, Cu, Pb, Hg, Ni, Se, Sn, V, and Zn) with breast cancer in women with age around 59 years. Laser ablation coupled to inductively coupled plasma mass spectrometry (LA-ICP-MS) was used to evaluate the changes of these elements along a hair strand while ICP-MS and inductively coupled plasma optical emission (ICP OES) was used to determine elements concentration in hair after sample decomposition. Scanning electron microscopy (SEM) and LA-ICP-MS were used to evaluate the effect on hair washing. Hair washing was done according to the procedure recommended by the International Atomic Energy Agency (IEAE). Hair strands were fixed on a glass slice with the aid of double-side tape and then analyzed. Hair samples without washing were analyzed in the same way. Changes in hair morphology were observed by the analyses with SEM after hair washing, while no changes in element concentration were observed when hair strands were analyzed by LA-ICP-MS after washing. For hair analysis by LA-ICP-MS, laser ablation conditions were fixed to 50 μm, 80 μm s-1 and 26.7 J cm-2, for the spot size, laser speed and ablation energy, respectively. Operational conditions of the ICP-MS instrument were adjusted in order to achieve the best sensitivity. Calibration in LA-ICP-MS was performed by using an internal standard (sulphur in the form of l-cysteine) and filter paper disc (17 mm diameter) as support of the analytes and internal standard. The way to add standard and internal standard solution to the filter paper was evaluate where the best results were achieved by the addition a mixture of the standard solution plus internal standard solution. For ICP-MS and ICP OES analyses, samples were decomposed with nitric acid and heating with the aid of microwave energy. From the analyzes by ICP-MS, ICP OES, and LA-ICP-MS it was observed that the concentrations of the elements in the samples varied, with Ba being the element present in the highest concentration in the samples. By analyzing the results obtained by ICP-MS and ICP OES with statistic tools, it was possible to observe higher concentrations of Ni, Cu, Cd and Sb in hair of women with cancer than women without cancer (Control individuals).Neste trabalho foi avaliada a relação de metaloestrógenos (Al, Sb, As, Ba, Cd, Cr, Co, Cu, Pb, Hg, Ni, Se, Sn, V e Zn) com o câncer de mama em mulheres com faixa etária média de 59 anos. Para tal, a espectrometria de massa com plasma indutivamente acoplado com ablação a laser (LA-ICP-MS) foi utilizada para a avaliar as variações dos níveis de concentração destes elementos ao longo de um fio de cabelo, enquanto que as técnicas de ICP-MS e espectrometria de emissão óptica com plasma indutivamente acoplado (ICP OES) foram utilizadas para determinar a concentração destes elementos no cabelo após decomposição. A microscopia eletrônica de varredura (MEV) e a LA-ICP-MS foram empregadas para avaliar o efeito da lavagem do cabelo, cujo procedimento foi de acordo com o recomendado pela Agência Internacional de Energia Atômica (IAEA). Os fios de cabelo foram fixados sobre uma lâmina de vidro com o auxílio de uma fita dupla face e, então, analisados. Através da MEV foi possível observar que a lavagem alterou a morfologia do cabelo, enquanto que por LA-ICP-MS foi possível verificar que não houve alteração significa da concentração dos elementos no cabelo. As condições de operação do laser foram estudadas, sendo fixadas em 50 μm, 80 μm s-1 e 26,7 J cm-2, para o tamanho do feixe de lazer, velocidade e energia de ablação, respectivamente. O instrumento de ICP-MS foi ajustado de forma a se obter a máxima sensibilidade. A calibração para a análise por LA-ICP-MS foi feita utilizando discos de papel filtro como suporte do analito, onde foi avaliada a forma de adição do padrão interno (l-cisteína). A melhor forma de adição do padrão interno, foi juntamente com a solução de calibração. Após ser observado que não havia necessidade de lavagem, comparando-se os resultados obtidos para cabelos lavados e não lavados, foi feita a determinação dos elementos nas amostras de cabelo de mulheres com e sem câncer de mama. Para a determinação da concentração dos elementos em cabelo por ICP OES e ICP-MS, as amostras foram decompostas com ácido nítrico concentrado e com aquecimento por radiação micro-ondas. A partir das análises por ICP-MS, ICP OES e LA-ICP-MS foi observado que as concentrações dos elementos nas amostras variaram, sendo o Ba o elemento presente em maior concentração nas amostras. Ao serem aplicados testes estatísticos nos dados obtidos por ICP OES e ICP-MS, foi possível observar diferenças entre o grupo de mulheres acometidas de câncer (amostra) e o grupo de mulheres sem a doença (controle) para os elementos Ni, Cu, Cd e Sb, onde estes apresentaram concentração superior para o grupo controle.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESporUniversidade Federal de Santa MariaCentro de Ciências Naturais e ExatasPrograma de Pós-Graduação em QuímicaUFSMBrasilQuímicaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessCâncer de mamaMetaloestrógenosLA-ICP-MSCabeloBreast cancerMetalloestrogensHairCNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICAAvaliação de metaloestrógenos em cabelo de mulheres com câncer de mama por LA-ICP-MSEvaluation of metalostrogens in hair of women with breast cancer by LA-ICP-MSinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisDressler, Valderi Luizhttp://lattes.cnpq.br/4054740296547580Krause, Luciana Maria FontanariNunes, Matheus Augusto Gonçalveshttp://lattes.cnpq.br/0115486529393136Rubin, Ana Caroline Vedovato10060000000060060060029c59cfe-a489-425c-88a5-18fdf7cbf258968cbfad-09a8-448d-b69d-b265db111071a310dec0-114a-4a8c-836d-d08756d1829983faeeac-e883-44bd-bf20-a9b146478365reponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMLICENSElicense.txtlicense.txttext/plain; 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dc.title.por.fl_str_mv |
Avaliação de metaloestrógenos em cabelo de mulheres com câncer de mama por LA-ICP-MS |
dc.title.alternative.eng.fl_str_mv |
Evaluation of metalostrogens in hair of women with breast cancer by LA-ICP-MS |
title |
Avaliação de metaloestrógenos em cabelo de mulheres com câncer de mama por LA-ICP-MS |
spellingShingle |
Avaliação de metaloestrógenos em cabelo de mulheres com câncer de mama por LA-ICP-MS Rubin, Ana Caroline Vedovato Câncer de mama Metaloestrógenos LA-ICP-MS Cabelo Breast cancer Metalloestrogens Hair CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA |
title_short |
Avaliação de metaloestrógenos em cabelo de mulheres com câncer de mama por LA-ICP-MS |
title_full |
Avaliação de metaloestrógenos em cabelo de mulheres com câncer de mama por LA-ICP-MS |
title_fullStr |
Avaliação de metaloestrógenos em cabelo de mulheres com câncer de mama por LA-ICP-MS |
title_full_unstemmed |
Avaliação de metaloestrógenos em cabelo de mulheres com câncer de mama por LA-ICP-MS |
title_sort |
Avaliação de metaloestrógenos em cabelo de mulheres com câncer de mama por LA-ICP-MS |
author |
Rubin, Ana Caroline Vedovato |
author_facet |
Rubin, Ana Caroline Vedovato |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Dressler, Valderi Luiz |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/4054740296547580 |
dc.contributor.referee1.fl_str_mv |
Krause, Luciana Maria Fontanari |
dc.contributor.referee2.fl_str_mv |
Nunes, Matheus Augusto Gonçalves |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/0115486529393136 |
dc.contributor.author.fl_str_mv |
Rubin, Ana Caroline Vedovato |
contributor_str_mv |
Dressler, Valderi Luiz Krause, Luciana Maria Fontanari Nunes, Matheus Augusto Gonçalves |
dc.subject.por.fl_str_mv |
Câncer de mama Metaloestrógenos LA-ICP-MS Cabelo |
topic |
Câncer de mama Metaloestrógenos LA-ICP-MS Cabelo Breast cancer Metalloestrogens Hair CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA |
dc.subject.eng.fl_str_mv |
Breast cancer Metalloestrogens Hair |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA |
description |
In this work, the relationship of metalloestrogens (Al, Sb, As, Ba, Cd, Cr, Co, Cu, Pb, Hg, Ni, Se, Sn, V, and Zn) with breast cancer in women with age around 59 years. Laser ablation coupled to inductively coupled plasma mass spectrometry (LA-ICP-MS) was used to evaluate the changes of these elements along a hair strand while ICP-MS and inductively coupled plasma optical emission (ICP OES) was used to determine elements concentration in hair after sample decomposition. Scanning electron microscopy (SEM) and LA-ICP-MS were used to evaluate the effect on hair washing. Hair washing was done according to the procedure recommended by the International Atomic Energy Agency (IEAE). Hair strands were fixed on a glass slice with the aid of double-side tape and then analyzed. Hair samples without washing were analyzed in the same way. Changes in hair morphology were observed by the analyses with SEM after hair washing, while no changes in element concentration were observed when hair strands were analyzed by LA-ICP-MS after washing. For hair analysis by LA-ICP-MS, laser ablation conditions were fixed to 50 μm, 80 μm s-1 and 26.7 J cm-2, for the spot size, laser speed and ablation energy, respectively. Operational conditions of the ICP-MS instrument were adjusted in order to achieve the best sensitivity. Calibration in LA-ICP-MS was performed by using an internal standard (sulphur in the form of l-cysteine) and filter paper disc (17 mm diameter) as support of the analytes and internal standard. The way to add standard and internal standard solution to the filter paper was evaluate where the best results were achieved by the addition a mixture of the standard solution plus internal standard solution. For ICP-MS and ICP OES analyses, samples were decomposed with nitric acid and heating with the aid of microwave energy. From the analyzes by ICP-MS, ICP OES, and LA-ICP-MS it was observed that the concentrations of the elements in the samples varied, with Ba being the element present in the highest concentration in the samples. By analyzing the results obtained by ICP-MS and ICP OES with statistic tools, it was possible to observe higher concentrations of Ni, Cu, Cd and Sb in hair of women with cancer than women without cancer (Control individuals). |
publishDate |
2021 |
dc.date.accessioned.fl_str_mv |
2021-11-30T12:56:49Z |
dc.date.available.fl_str_mv |
2021-11-30T12:56:49Z |
dc.date.issued.fl_str_mv |
2021-05-07 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/23066 |
url |
http://repositorio.ufsm.br/handle/1/23066 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.cnpq.fl_str_mv |
100600000000 |
dc.relation.confidence.fl_str_mv |
600 600 600 |
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dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Naturais e Exatas |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Química |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Química |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Naturais e Exatas |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
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