Expressão de ISG15 em leucócitos de ovelhas suplementadas com progesterona no anestro estacional
Ano de defesa: | 2020 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Santa Maria
Centro de Ciências Rurais |
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Medicina Veterinária
|
Departamento: |
Medicina Veterinária
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.ufsm.br/handle/1/22262 |
Resumo: | The aim of this study was to evaluate the effect of supplementation with exogenous progesterone on the expression of ISG15 in leukocytes and concentration of serum progesterone, on days 10 and 16 of early pregnancy in ewes. The ewes were randomly allocated into two groups: Control Group (GC) and Group P4 (P4G), during seasonal anestrous period and breeding season. The groups were submitted to estrous cycle manipulation protocol. In both periods, P4G received a dose of injectable progesterone on day 3 after mating (25 mg), while the CG received the same vehicle volume. During the non-breeding season, the expression of ISG15 in leukocytes at day 10 did not differ between the groups, P4G and GC (p> 0.05). On the other hand, the expression of ISG15 on day 16 is higher in the CG, when compared to the P4G (p <0.05). During breeding season, the results of ISG15 gene expression did not show differences between groups on days 10 and 16. During non-breeding season, on day 10, the P4G showed lower concentrations of serum progesterone when compared to the CG. On day 16, serum progesterone concentration also decreased in P4G compared to CG. During the breeding season, serum progesterone at D10 did not differ between the groups, P4G and GC (p> 0.05). On the contrary, P4G showed higher concentrations of circulating progesterone when compared to GC on day 16. The results indicate that progesterone supplementation at the beginning of pregnancy in sheep in the seasonal anestrus is not effective in increasing endocrine signaling in maternal recognition of pregnancy or raising serum progesterone, however, it increases serum progesterone during the reproductive season without changing the relative expression of ISG15 in leukocytes. |
id |
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network_name_str |
Manancial - Repositório Digital da UFSM |
repository_id_str |
|
spelling |
2021-09-22T18:42:51Z2021-09-22T18:42:51Z2020-02-20http://repositorio.ufsm.br/handle/1/22262The aim of this study was to evaluate the effect of supplementation with exogenous progesterone on the expression of ISG15 in leukocytes and concentration of serum progesterone, on days 10 and 16 of early pregnancy in ewes. The ewes were randomly allocated into two groups: Control Group (GC) and Group P4 (P4G), during seasonal anestrous period and breeding season. The groups were submitted to estrous cycle manipulation protocol. In both periods, P4G received a dose of injectable progesterone on day 3 after mating (25 mg), while the CG received the same vehicle volume. During the non-breeding season, the expression of ISG15 in leukocytes at day 10 did not differ between the groups, P4G and GC (p> 0.05). On the other hand, the expression of ISG15 on day 16 is higher in the CG, when compared to the P4G (p <0.05). During breeding season, the results of ISG15 gene expression did not show differences between groups on days 10 and 16. During non-breeding season, on day 10, the P4G showed lower concentrations of serum progesterone when compared to the CG. On day 16, serum progesterone concentration also decreased in P4G compared to CG. During the breeding season, serum progesterone at D10 did not differ between the groups, P4G and GC (p> 0.05). On the contrary, P4G showed higher concentrations of circulating progesterone when compared to GC on day 16. The results indicate that progesterone supplementation at the beginning of pregnancy in sheep in the seasonal anestrus is not effective in increasing endocrine signaling in maternal recognition of pregnancy or raising serum progesterone, however, it increases serum progesterone during the reproductive season without changing the relative expression of ISG15 in leukocytes.O objetivo deste trabalho foi avaliar a expressão gênica de ISG15 em leucócitos e progesterona sérica, nos dias 10 e 16 da gestação inicial de ovelhas suplementadas com progesterona. As ovelhas foram alocadas aleatoriamente em dois grupos: Grupo Controle (GC) e Grupo P4 (P4G) durante o período de anestro estacional e na estação reprodutiva. Os grupos foram submetidos a um protocolo de manipulação do ciclo estral. Nos dois períodos, o P4G recebeu uma dose de progesterona injetável no dia 3 após o acasalamento (25 mg), enquanto o GC recebeu o mesmo volume de veículo. A expressão do ISG15 nos leucócitos no D10 não diferiu entre os grupos, P4G e GC (p> 0,05) no anestro e na estação reprodutiva. Por outro lado, no anestro a expressão de ISG15 no dia 16 é maior no GC, quando comparada ao P4G (p <0,05). Os resultados da expressão gênica de ISG15 não mostraram diferenças entre os grupos nos dias 10 e 16 na estação reprodutiva. Durante o anestro, o P4G no dia 10 apresentou menores concentrações de progesterona sérica quando comparadas ao GC. No dia 16, a concentração sérica de progesterona diminuiu no P4G em relação ao GC. Durante a estação reprodutiva, a progesterona sérica no dia 10 não diferiu entre os grupos, P4G e GC (p> 0,05). Ao contrário, P4G apresentou maiores concentrações de progesterona circulante quando comparadas ao GC no dia 16. Os resultados indicam que a suplementação de progesterona no início da gestação em ovelhas no anestro estacional não é eficaz em melhorar a sinalização endócrina do reconhecimento materno da gestação além de diminuir a P4 sérica, no entanto, aumenta a P4 sérica durante a estação reprodutiva sem alterar a expressão relativa de ISG15 nos leucócitos.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESporUniversidade Federal de Santa MariaCentro de Ciências RuraisPrograma de Pós-Graduação em Medicina VeterináriaUFSMBrasilMedicina VeterináriaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessProgesteronaOvelhasGestaçãoISG15LeucócitosProgesteroneEwesPregnancyLeukocytesCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIAExpressão de ISG15 em leucócitos de ovelhas suplementadas com progesterona no anestro estacionalISG15 gene expression in leukocytes of progesterone-supplemented ewes during non-breeding seasoninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisAntoniazzi, Alfredo Quiteshttp://lattes.cnpq.br/7258679373419746Mesquita, Fernando SilveiraRovani, Monique Tomazelehttp://lattes.cnpq.br/2991726111428379Manta, Manuela Wolker500500000007600600600a338fc55-084a-47a0-8cef-889209277d3d0a128e3b-164e-4a34-9d0f-3a4483b87fc84cd5e569-cc1a-47c5-8ad0-c8161879aedd282e1264-1142-4b7b-ab52-e225f8e37eadreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALDIS_PPGMV_2020_MANTA_MANUELA.pdfDIS_PPGMV_2020_MANTA_MANUELA.pdfDissertação de Mestradoapplication/pdf583372http://repositorio.ufsm.br/bitstream/1/22262/1/DIS_PPGMV_2020_MANTA_MANUELA.pdfb08025afa5da527279979a4a7461b0e6MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; 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dc.title.por.fl_str_mv |
Expressão de ISG15 em leucócitos de ovelhas suplementadas com progesterona no anestro estacional |
dc.title.alternative.eng.fl_str_mv |
ISG15 gene expression in leukocytes of progesterone-supplemented ewes during non-breeding season |
title |
Expressão de ISG15 em leucócitos de ovelhas suplementadas com progesterona no anestro estacional |
spellingShingle |
Expressão de ISG15 em leucócitos de ovelhas suplementadas com progesterona no anestro estacional Manta, Manuela Wolker Progesterona Ovelhas Gestação ISG15 Leucócitos Progesterone Ewes Pregnancy Leukocytes CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Expressão de ISG15 em leucócitos de ovelhas suplementadas com progesterona no anestro estacional |
title_full |
Expressão de ISG15 em leucócitos de ovelhas suplementadas com progesterona no anestro estacional |
title_fullStr |
Expressão de ISG15 em leucócitos de ovelhas suplementadas com progesterona no anestro estacional |
title_full_unstemmed |
Expressão de ISG15 em leucócitos de ovelhas suplementadas com progesterona no anestro estacional |
title_sort |
Expressão de ISG15 em leucócitos de ovelhas suplementadas com progesterona no anestro estacional |
author |
Manta, Manuela Wolker |
author_facet |
Manta, Manuela Wolker |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Antoniazzi, Alfredo Quites |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/7258679373419746 |
dc.contributor.referee1.fl_str_mv |
Mesquita, Fernando Silveira |
dc.contributor.referee2.fl_str_mv |
Rovani, Monique Tomazele |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/2991726111428379 |
dc.contributor.author.fl_str_mv |
Manta, Manuela Wolker |
contributor_str_mv |
Antoniazzi, Alfredo Quites Mesquita, Fernando Silveira Rovani, Monique Tomazele |
dc.subject.por.fl_str_mv |
Progesterona Ovelhas Gestação ISG15 Leucócitos |
topic |
Progesterona Ovelhas Gestação ISG15 Leucócitos Progesterone Ewes Pregnancy Leukocytes CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
dc.subject.eng.fl_str_mv |
Progesterone Ewes Pregnancy Leukocytes |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
The aim of this study was to evaluate the effect of supplementation with exogenous progesterone on the expression of ISG15 in leukocytes and concentration of serum progesterone, on days 10 and 16 of early pregnancy in ewes. The ewes were randomly allocated into two groups: Control Group (GC) and Group P4 (P4G), during seasonal anestrous period and breeding season. The groups were submitted to estrous cycle manipulation protocol. In both periods, P4G received a dose of injectable progesterone on day 3 after mating (25 mg), while the CG received the same vehicle volume. During the non-breeding season, the expression of ISG15 in leukocytes at day 10 did not differ between the groups, P4G and GC (p> 0.05). On the other hand, the expression of ISG15 on day 16 is higher in the CG, when compared to the P4G (p <0.05). During breeding season, the results of ISG15 gene expression did not show differences between groups on days 10 and 16. During non-breeding season, on day 10, the P4G showed lower concentrations of serum progesterone when compared to the CG. On day 16, serum progesterone concentration also decreased in P4G compared to CG. During the breeding season, serum progesterone at D10 did not differ between the groups, P4G and GC (p> 0.05). On the contrary, P4G showed higher concentrations of circulating progesterone when compared to GC on day 16. The results indicate that progesterone supplementation at the beginning of pregnancy in sheep in the seasonal anestrus is not effective in increasing endocrine signaling in maternal recognition of pregnancy or raising serum progesterone, however, it increases serum progesterone during the reproductive season without changing the relative expression of ISG15 in leukocytes. |
publishDate |
2020 |
dc.date.issued.fl_str_mv |
2020-02-20 |
dc.date.accessioned.fl_str_mv |
2021-09-22T18:42:51Z |
dc.date.available.fl_str_mv |
2021-09-22T18:42:51Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
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http://repositorio.ufsm.br/handle/1/22262 |
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http://repositorio.ufsm.br/handle/1/22262 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.cnpq.fl_str_mv |
500500000007 |
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600 600 600 |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Rurais |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Medicina Veterinária |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Medicina Veterinária |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Rurais |
dc.source.none.fl_str_mv |
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