Índices produtivos de leitões em fase de creche alimentados com dietas contendo bioflavonóides e ácido ascórbico (extratos vegetais)
Ano de defesa: | 2015 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Santa Maria
|
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Medicina Veterinária
|
Departamento: |
Medicina Veterinária
|
País: |
BR
|
Palavras-chave em Português: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.ufsm.br/handle/1/10205 |
Resumo: | The objective of this study was to evaluate the performance, the microbiological profile, the enzyme profile and the detection of pathogenic strains by multiplex PCR of 40 weaned piglets (females and males). The experimental desing was completely randomized with fours treatments, five replicates and sex as a bloking factor. The treatments consistes of: T1 (control); T2 (Plant Extract (EV) (500 ppm)); T3 (Amoxicilin (A) (20 mg Kg-1) and T4 (A (20 mg kg-1)+EV (500ppm). There was no effect (P>0.01) between treatments for initial and final weight and gain daily weight, but the males in the control group had a mean daily consumption of 1.8% over diet (P<0.01) to the other treatments. The total count control of bacterial colonies was 35.9%, 70.9% and 63.8% higher (P<0.01) to treatment with A, EV+A and EV, respectively. In the midst MacConkey, the treated group A was 88.44%, 91.78% and 56.50%, higher (P<0.01) compared to EV+A, EV and control, respectively. The antibiogram 48 stool samples showed that Amoxicilin disk was 85.7%, 72.7%, 44.5% and 100% resistant in the control treatment, EV, A and EV+A, respectively. The bioflavonoids and ascorbic acid and the interaction with Amoxicilin not change the performance of pigs in the nursery phase, but reduce the colony count bacterianas. By the dosing biochemical CK, AST (U L-1), AST, TBARS and the frequency of fimbriae genes (F41, F6 (R 987), F4 (K88), F18 and F5 (K99) and toxins (LT, STx, STa and STb) of E. coli strains isolated from pigs with diarrhea using multiplex PCR with primers specific to these genes. Of the 64 samples 55 (85.93%) had genes for fimbriae and toxins, 23 (35.93%) had only genes for fimbriae and only two samples had genes for toxins only. The frequency of the detected virulence factors alone or in combination with other factors were: F41 (10.93%), F6 (21.87%), F4 (37.5%), F18 (70.31%), LT (39.6%), Stx (42.18%), STa (60.93%) and STb (75%). In all 64 samples was not found any of virulence factors for F5 (K99). The use of bioflavonoids and ascorbic acid did not change the performance of pigs in the weaned piglets. The use of plant extracts and associated with Amoxicilin reduces the count of bacterial colonies. Showed high resistance of the samples studied to Amoxicilin, the Neomycin and Norfloxacin. The antimicrobial Cefepine, ceftriaxone, Merepenem were more efficient in inhibiting the growth of E. coli strains isolated from pigs supplemented with ascorbic acid and bioflavonoid. The EV supplementation to the diets of weaned piglets does not alter the enzymatic profiles (ALT, AST), muscle (CK) and oxidative (TBARS). Animals receiving IV or its interaction with Amoxicilin presented to fimbriae genes and toxins. EV supplementation to weanling pigs a diet did not cause lack genes for the presence of fimbriae and toxins. The presence of genes for fimbriae and toxins does not determine the occurrence of diarrhea, as they may be present, but are not being expressed. |
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2017-06-142017-06-142015-02-26OLIVEIRA, Magali Fernandes de. Production indices of piglets in nursery phase fed wtih diets containing bioflavanoids and ascorbic acid (plants extracts). 2015. 140 f. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2015.http://repositorio.ufsm.br/handle/1/10205The objective of this study was to evaluate the performance, the microbiological profile, the enzyme profile and the detection of pathogenic strains by multiplex PCR of 40 weaned piglets (females and males). The experimental desing was completely randomized with fours treatments, five replicates and sex as a bloking factor. The treatments consistes of: T1 (control); T2 (Plant Extract (EV) (500 ppm)); T3 (Amoxicilin (A) (20 mg Kg-1) and T4 (A (20 mg kg-1)+EV (500ppm). There was no effect (P>0.01) between treatments for initial and final weight and gain daily weight, but the males in the control group had a mean daily consumption of 1.8% over diet (P<0.01) to the other treatments. The total count control of bacterial colonies was 35.9%, 70.9% and 63.8% higher (P<0.01) to treatment with A, EV+A and EV, respectively. In the midst MacConkey, the treated group A was 88.44%, 91.78% and 56.50%, higher (P<0.01) compared to EV+A, EV and control, respectively. The antibiogram 48 stool samples showed that Amoxicilin disk was 85.7%, 72.7%, 44.5% and 100% resistant in the control treatment, EV, A and EV+A, respectively. The bioflavonoids and ascorbic acid and the interaction with Amoxicilin not change the performance of pigs in the nursery phase, but reduce the colony count bacterianas. By the dosing biochemical CK, AST (U L-1), AST, TBARS and the frequency of fimbriae genes (F41, F6 (R 987), F4 (K88), F18 and F5 (K99) and toxins (LT, STx, STa and STb) of E. coli strains isolated from pigs with diarrhea using multiplex PCR with primers specific to these genes. Of the 64 samples 55 (85.93%) had genes for fimbriae and toxins, 23 (35.93%) had only genes for fimbriae and only two samples had genes for toxins only. The frequency of the detected virulence factors alone or in combination with other factors were: F41 (10.93%), F6 (21.87%), F4 (37.5%), F18 (70.31%), LT (39.6%), Stx (42.18%), STa (60.93%) and STb (75%). In all 64 samples was not found any of virulence factors for F5 (K99). The use of bioflavonoids and ascorbic acid did not change the performance of pigs in the weaned piglets. The use of plant extracts and associated with Amoxicilin reduces the count of bacterial colonies. Showed high resistance of the samples studied to Amoxicilin, the Neomycin and Norfloxacin. The antimicrobial Cefepine, ceftriaxone, Merepenem were more efficient in inhibiting the growth of E. coli strains isolated from pigs supplemented with ascorbic acid and bioflavonoid. The EV supplementation to the diets of weaned piglets does not alter the enzymatic profiles (ALT, AST), muscle (CK) and oxidative (TBARS). Animals receiving IV or its interaction with Amoxicilin presented to fimbriae genes and toxins. EV supplementation to weanling pigs a diet did not cause lack genes for the presence of fimbriae and toxins. The presence of genes for fimbriae and toxins does not determine the occurrence of diarrhea, as they may be present, but are not being expressed.O objetivo deste trabalho foi avaliar o desempenho, o perfil microbiológico, o perfil enzimático e a detecção de cepas patogênicas pela PCR multiplex de 40 leitões (fêmeas e machos) em fase de creche. O delineamento experimental foi inteiramente casualizado, com quatro tratamentos, cinco repetições e sexo como fator de bloqueamento. Os tratamentos foram distribuídos em: T1 (controle); T2 (Extrato Vegetal (EV) (500ppm)); T3 (Amoxicilina (A) (20mg kg-1) e T4 (A (20mg kg-1) + EV (500ppm). Não houve influência (P>0,01), entre os tratamentos para peso inicial e final e para ganho diário de peso, porém os machos do grupo controle apresentaram consumo diário médio de ração 1,8% superior (P<0,01) aos demais tratamentos. A contagem total de colônias bacterianas do grupo controle foi 35,9%, 70,9% e 63,8% superior (P<0,01) aos tratamentos com A, EV+A e EV, respectivamente. Em meio MacConkey, o grupo tratado com A foi 88,44%, 91,78% e 56,50%, superior (P<0,01) se comparados com EV+A, EV e controle, respectivamente. O antibiograma de 48 amostras de fezes mostrou que o disco de Amoxicilina foi 85,7%, 72,7%, 44,5% e 100% resistente nos tratamentos controle, EV, A e EV+A, respectivamente. Quanto a dosagem bioquímica CK, AST (U L-1), AST, TBARS e a frequência dos genes de fímbrias (F41, F6 (987 P), F4 (K88), F18 e F5 (K99) e toxinas (LT, STx, LTa e LTb) de cepas de E. coli isoladas de leitões com diarreia usando a técnica de PCR multiplex com primers específicos para esses genes. Das 64 amostras 55 (85,93%) apresentaram genes para fímbrias e toxinas, 23 (35,93%) apresentaram genes somente para fímbrias e somente duas amostras apresentaram genes somente para toxinas. A frequência dos fatores de virulência detectada isolada ou em associação com outros fatores foi: F41 (10,93%), F6 (21,87%), F4 (37,5%), F18 (70,31%), LT (39,6%), Stx (42,18%), STa (60,93%) e STb (75%). Em todas as 64 amostras não foi encontrada nenhum dos fatores de virulência para F5 (k99). O uso de bioflavonoides e ácido ascórbico não afetou o desempenho de suínos na fase de creche. O uso de extratos vegetais e associados a Amoxicilina reduz a contagem de colônias bacterianas. Foi observada alta resistência das amostras estudadas à Amoxicilina, a Neomicina e a Norfloxacina. Os antimicrobianos Cefepine, Ceftriaxona e Merepenem foram os mais eficientes na inibição do crescimento das amostras de E. coli isoladas de suínos suplementados com ácido ascórbico e bioflavonóides. A suplementação de EV às dietas de leitões desmamados não altera os perfis enzimáticos (ALT, AST), muscular (CK) e oxidativo (TBARS). Os animais que receberam EV ou sua interação com amoxicilina apresentou genes para fímbrias e toxinas. A suplementação de EV às dietas de leitões desmamados não determinou a ausência de genes para a presença de fímbrias e toxinas. A presença de genes para fímbrias e toxinas não determina a ocorrência de diarreia, pois eles podem estar presentes, mas não estarem sendo expressos.application/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em Medicina VeterináriaUFSMBRMedicina VeterináriaLeitõesExtrato vegetalEstresse oxidativoDesmameCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIAÍndices produtivos de leitões em fase de creche alimentados com dietas contendo bioflavonóides e ácido ascórbico (extratos vegetais)Production indices of piglets in nursery phase fed wtih diets containing bioflavanoids and ascorbic acid (plants extracts)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisRossi, Carlos Augusto Rigonhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4774831T7Soares, Marcelohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781274Y5Lucca, Walterhttp://lattes.cnpq.br/6341457519243415http://lattes.cnpq.br/2774788956442557Oliveira, Magali Fernandes de50050000000740030030050030069c8ec23-926a-40b3-b11e-d91c1c81f1a9c3f58a56-6625-455d-8695-4b4fe6af5fd69dfc4998-478f-4cdb-85d0-403a8cdc729003ab8a97-92c0-4b8a-8d2b-0a1a305a3abdinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALOLIVEIRA, MAGALI FERNANDES DE.pdfapplication/pdf4305803http://repositorio.ufsm.br/bitstream/1/10205/1/OLIVEIRA%2c%20MAGALI%20FERNANDES%20DE.pdfd111985da94f130258d7f23d55cc9446MD51TEXTOLIVEIRA, MAGALI FERNANDES DE.pdf.txtOLIVEIRA, MAGALI FERNANDES DE.pdf.txtExtracted texttext/plain247293http://repositorio.ufsm.br/bitstream/1/10205/2/OLIVEIRA%2c%20MAGALI%20FERNANDES%20DE.pdf.txta21d5c0d5cccca1da9b6d3e88afdb8e0MD52THUMBNAILOLIVEIRA, MAGALI FERNANDES DE.pdf.jpgOLIVEIRA, MAGALI FERNANDES DE.pdf.jpgIM Thumbnailimage/jpeg4708http://repositorio.ufsm.br/bitstream/1/10205/3/OLIVEIRA%2c%20MAGALI%20FERNANDES%20DE.pdf.jpgd57814eb9dbcb2c046f41d022e731ec9MD531/102052017-07-25 12:01:35.385oai:repositorio.ufsm.br:1/10205Repositório Institucionalhttp://repositorio.ufsm.br/PUBhttp://repositorio.ufsm.br/oai/requestopendoar:39132017-07-25T15:01:35Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Índices produtivos de leitões em fase de creche alimentados com dietas contendo bioflavonóides e ácido ascórbico (extratos vegetais) |
dc.title.alternative.eng.fl_str_mv |
Production indices of piglets in nursery phase fed wtih diets containing bioflavanoids and ascorbic acid (plants extracts) |
title |
Índices produtivos de leitões em fase de creche alimentados com dietas contendo bioflavonóides e ácido ascórbico (extratos vegetais) |
spellingShingle |
Índices produtivos de leitões em fase de creche alimentados com dietas contendo bioflavonóides e ácido ascórbico (extratos vegetais) Oliveira, Magali Fernandes de Leitões Extrato vegetal Estresse oxidativo Desmame CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Índices produtivos de leitões em fase de creche alimentados com dietas contendo bioflavonóides e ácido ascórbico (extratos vegetais) |
title_full |
Índices produtivos de leitões em fase de creche alimentados com dietas contendo bioflavonóides e ácido ascórbico (extratos vegetais) |
title_fullStr |
Índices produtivos de leitões em fase de creche alimentados com dietas contendo bioflavonóides e ácido ascórbico (extratos vegetais) |
title_full_unstemmed |
Índices produtivos de leitões em fase de creche alimentados com dietas contendo bioflavonóides e ácido ascórbico (extratos vegetais) |
title_sort |
Índices produtivos de leitões em fase de creche alimentados com dietas contendo bioflavonóides e ácido ascórbico (extratos vegetais) |
author |
Oliveira, Magali Fernandes de |
author_facet |
Oliveira, Magali Fernandes de |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Rossi, Carlos Augusto Rigon |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4774831T7 |
dc.contributor.referee1.fl_str_mv |
Soares, Marcelo |
dc.contributor.referee1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781274Y5 |
dc.contributor.referee2.fl_str_mv |
Lucca, Walter |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/6341457519243415 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/2774788956442557 |
dc.contributor.author.fl_str_mv |
Oliveira, Magali Fernandes de |
contributor_str_mv |
Rossi, Carlos Augusto Rigon Soares, Marcelo Lucca, Walter |
dc.subject.por.fl_str_mv |
Leitões Extrato vegetal Estresse oxidativo Desmame |
topic |
Leitões Extrato vegetal Estresse oxidativo Desmame CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
The objective of this study was to evaluate the performance, the microbiological profile, the enzyme profile and the detection of pathogenic strains by multiplex PCR of 40 weaned piglets (females and males). The experimental desing was completely randomized with fours treatments, five replicates and sex as a bloking factor. The treatments consistes of: T1 (control); T2 (Plant Extract (EV) (500 ppm)); T3 (Amoxicilin (A) (20 mg Kg-1) and T4 (A (20 mg kg-1)+EV (500ppm). There was no effect (P>0.01) between treatments for initial and final weight and gain daily weight, but the males in the control group had a mean daily consumption of 1.8% over diet (P<0.01) to the other treatments. The total count control of bacterial colonies was 35.9%, 70.9% and 63.8% higher (P<0.01) to treatment with A, EV+A and EV, respectively. In the midst MacConkey, the treated group A was 88.44%, 91.78% and 56.50%, higher (P<0.01) compared to EV+A, EV and control, respectively. The antibiogram 48 stool samples showed that Amoxicilin disk was 85.7%, 72.7%, 44.5% and 100% resistant in the control treatment, EV, A and EV+A, respectively. The bioflavonoids and ascorbic acid and the interaction with Amoxicilin not change the performance of pigs in the nursery phase, but reduce the colony count bacterianas. By the dosing biochemical CK, AST (U L-1), AST, TBARS and the frequency of fimbriae genes (F41, F6 (R 987), F4 (K88), F18 and F5 (K99) and toxins (LT, STx, STa and STb) of E. coli strains isolated from pigs with diarrhea using multiplex PCR with primers specific to these genes. Of the 64 samples 55 (85.93%) had genes for fimbriae and toxins, 23 (35.93%) had only genes for fimbriae and only two samples had genes for toxins only. The frequency of the detected virulence factors alone or in combination with other factors were: F41 (10.93%), F6 (21.87%), F4 (37.5%), F18 (70.31%), LT (39.6%), Stx (42.18%), STa (60.93%) and STb (75%). In all 64 samples was not found any of virulence factors for F5 (K99). The use of bioflavonoids and ascorbic acid did not change the performance of pigs in the weaned piglets. The use of plant extracts and associated with Amoxicilin reduces the count of bacterial colonies. Showed high resistance of the samples studied to Amoxicilin, the Neomycin and Norfloxacin. The antimicrobial Cefepine, ceftriaxone, Merepenem were more efficient in inhibiting the growth of E. coli strains isolated from pigs supplemented with ascorbic acid and bioflavonoid. The EV supplementation to the diets of weaned piglets does not alter the enzymatic profiles (ALT, AST), muscle (CK) and oxidative (TBARS). Animals receiving IV or its interaction with Amoxicilin presented to fimbriae genes and toxins. EV supplementation to weanling pigs a diet did not cause lack genes for the presence of fimbriae and toxins. The presence of genes for fimbriae and toxins does not determine the occurrence of diarrhea, as they may be present, but are not being expressed. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015-02-26 |
dc.date.accessioned.fl_str_mv |
2017-06-14 |
dc.date.available.fl_str_mv |
2017-06-14 |
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dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
OLIVEIRA, Magali Fernandes de. Production indices of piglets in nursery phase fed wtih diets containing bioflavanoids and ascorbic acid (plants extracts). 2015. 140 f. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2015. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/10205 |
identifier_str_mv |
OLIVEIRA, Magali Fernandes de. Production indices of piglets in nursery phase fed wtih diets containing bioflavanoids and ascorbic acid (plants extracts). 2015. 140 f. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2015. |
url |
http://repositorio.ufsm.br/handle/1/10205 |
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Universidade Federal de Santa Maria |
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