Avaliação de biomarcadores inflamatórios, oxidativos e vasculares em pacientes com hipercolesterolemia
Ano de defesa: | 2011 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Santa Maria
|
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Ciências Farmacêuticas
|
Departamento: |
Análises Clínicas e Toxicológicas
|
País: |
BR
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.ufsm.br/handle/1/5930 |
Resumo: | Hypercholesterolemia is a determining factor for the development of atherosclerosis, which is considered the main cause of cardiovascular diseases. In Brazil, as in most developed countries, cardiovascular diseases are the leading cause of death. Several mechanisms are involved in the development of atheromatous plaque thus the purpose of this study was to evaluate the association between hypercholesterolemia and inflammatory, vascular, and oxidative biomarkers as well as to develop and validate an analytical method for the automated measurement of nitrite, a metabolite of the oxide oxide (NO) in plasma samples. In the first phase of this study an analytical method for automated measurement of plasma nitrite was developed and validated. This method was precise (r2=0,9998, P<0,001), linear, simple, inexpensive, and applicable to routine monitoring. In phase 2, inflammatory, vascular and oxidative markers were assessed. The groups were: group with hypercholesterolemia (LDL-C ≥ 160 mg / dl) and normocholesterolemic group (LDL-C ≤ 130 mg / dL). Results showed that levels of total cholesterol, LDL-C, HDL-C, triglycerides, apolipoprotein A, apolipoprotein B, advanced oxidation protein products (AOPP), interleukin-6 (IL-6) and D-dimer were significantly higher in hypercholesterolemic patients, while levels of thiol grouping (-SH) and nitrate / nitrite (NOx) were lower in this group. There were also significant correlations for LDL-C and IL-6 (r = 0.693, P <0.0001), LDL-C and NOx (r = -0.314, P <0.05) and LDL-C and - SH (r = -0.327, P <0.05). Thus, hypercholesterolemia promotes increased oxidative stress, inflammation, and fibrinolysis in atherosclerosis. |
id |
UFSM-20_5387e7f2fe90e1af47eab6b6ce5b724e |
---|---|
oai_identifier_str |
oai:repositorio.ufsm.br:1/5930 |
network_acronym_str |
UFSM-20 |
network_name_str |
Manancial - Repositório Digital da UFSM |
repository_id_str |
|
spelling |
2013-05-152013-05-152011-03-21PEREIRA, Renata da Silva. EVALUATION OF INFLAMMATORY, OXIDATIVE, AND VASCULAR BIOMARKERS IN PATIENTS WITH HIPERCHOLESTEROLEMIA. 2011. 70 f. Dissertação (Mestrado em Farmacologia) - Universidade Federal de Santa Maria, Santa Maria, 2011.http://repositorio.ufsm.br/handle/1/5930Hypercholesterolemia is a determining factor for the development of atherosclerosis, which is considered the main cause of cardiovascular diseases. In Brazil, as in most developed countries, cardiovascular diseases are the leading cause of death. Several mechanisms are involved in the development of atheromatous plaque thus the purpose of this study was to evaluate the association between hypercholesterolemia and inflammatory, vascular, and oxidative biomarkers as well as to develop and validate an analytical method for the automated measurement of nitrite, a metabolite of the oxide oxide (NO) in plasma samples. In the first phase of this study an analytical method for automated measurement of plasma nitrite was developed and validated. This method was precise (r2=0,9998, P<0,001), linear, simple, inexpensive, and applicable to routine monitoring. In phase 2, inflammatory, vascular and oxidative markers were assessed. The groups were: group with hypercholesterolemia (LDL-C ≥ 160 mg / dl) and normocholesterolemic group (LDL-C ≤ 130 mg / dL). Results showed that levels of total cholesterol, LDL-C, HDL-C, triglycerides, apolipoprotein A, apolipoprotein B, advanced oxidation protein products (AOPP), interleukin-6 (IL-6) and D-dimer were significantly higher in hypercholesterolemic patients, while levels of thiol grouping (-SH) and nitrate / nitrite (NOx) were lower in this group. There were also significant correlations for LDL-C and IL-6 (r = 0.693, P <0.0001), LDL-C and NOx (r = -0.314, P <0.05) and LDL-C and - SH (r = -0.327, P <0.05). Thus, hypercholesterolemia promotes increased oxidative stress, inflammation, and fibrinolysis in atherosclerosis.A hipercolesterolemia é um fator determinante para o desenvolvimento da aterosclerose, que é considerada a principal causa de doenças cardiovasculares. No Brasil, assim como na maior parte dos países desenvolvidos, as doenças cardiovasculares representam a principal causa de morbimortalidade. Vários mecanismos estão envolvidos no desenvolvimento da placa de ateroma, assim o objetivo deste estudo foi avaliar a associação entre a hipercolesterolemia e biomarcadores inflamatórios, oxidativos e vasculares, bem como desenvolver e validar um método analítico automatizado para a mensuração de nitrito, um metabólito do óxido nítrico (NO), em amostras de plasma. Na fase 1 deste estudo, foi desenvolvido e validado um método analítico automatizado para dosagem de nitrito plasmático. Este método foi preciso, linear (r2=0,9998, P<0,001), simples, de baixo custo e aplicável a rotina laboratorial. Na fase 2, foram avaliados marcadores inflamatórios, oxidativos e vasculares. Os grupos estudados foram: grupo hipercolesterolêmicos (LDL-C ≥ 160 mg/dL) e normocolesterolêmicos (LDL-C ≤ 130 mg/dL). Os resultados demonstraram que níveis de colesterol total, LDL-C, HDL-C, triglicérides, apoliproteína A, apoliproteína B, produtos protéicos da oxidação avançada (AOPP), interleucina-6 (IL-6) e D-dímero foram significativamente maiores nos pacientes hipercolesterolêmicos, enquanto os níveis de grupamento tiol (-SH) e nitrato/nitrito (NOx) foram inferiores neste grupo. Foram ainda observadas correlações significativas para o LDLC e IL-6 (r = 0,693, P <0,001), LDL-C e de NOx (r = -0,314, P <0,05) e LDL-C e SH (r = - 0,327, P <0,05). Dessa forma, a hipercolesterolemia promove o aumento do estresse oxidativo, inflamação e fibrinólise durante a aterosclerose.application/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em Ciências FarmacêuticasUFSMBRAnálises Clínicas e ToxicológicasHipercolesterolemiaAteroscleroseInflamaçãoEstresse oxidativoFibrinóliseMétodo automatizadoÓxido nítricoHypercholesterolemiaAtherosclerosisInflammationOxidative stressFibrinolysisAutomated methodNitric oxideCNPQ::CIENCIAS DA SAUDE::FARMACIAAvaliação de biomarcadores inflamatórios, oxidativos e vasculares em pacientes com hipercolesterolemiaEvaluation of inflammatory, oxidative, and vascular biomarkers in patients with hipercholesterolemiainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisMoresco, Rafael Noalhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4765801Z9Augusti, Paula Rossinihttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4778049T7Brandão, Ricardohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4779496T3Pereira, Renata da Silva2010000000004005003005005007a69f2ef-75ae-4e01-b1d1-8295e8c94f97964d0fcb-db67-4cef-977b-ae0d717b866cf181ae30-4746-410b-acb9-e5e5c764a340e6617091-34a2-401c-87dc-5d8877667a00info:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALPEREIRA, RENATA DA SILVA.pdfapplication/pdf1274756http://repositorio.ufsm.br/bitstream/1/5930/1/PEREIRA%2c%20RENATA%20DA%20SILVA.pdf038907b63d781ba889e682fb5fc09626MD51TEXTPEREIRA, RENATA DA SILVA.pdf.txtPEREIRA, RENATA DA SILVA.pdf.txtExtracted texttext/plain127519http://repositorio.ufsm.br/bitstream/1/5930/2/PEREIRA%2c%20RENATA%20DA%20SILVA.pdf.txt869941ac0df27d52c606220f0fab7da6MD52THUMBNAILPEREIRA, RENATA DA SILVA.pdf.jpgPEREIRA, RENATA DA SILVA.pdf.jpgIM Thumbnailimage/jpeg4875http://repositorio.ufsm.br/bitstream/1/5930/3/PEREIRA%2c%20RENATA%20DA%20SILVA.pdf.jpg334ab4ae535964f8fd39e1cf139fa076MD531/59302022-10-17 12:04:30.992oai:repositorio.ufsm.br:1/5930Repositório Institucionalhttp://repositorio.ufsm.br/PUBhttp://repositorio.ufsm.br/oai/requestopendoar:39132022-10-17T15:04:30Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Avaliação de biomarcadores inflamatórios, oxidativos e vasculares em pacientes com hipercolesterolemia |
dc.title.alternative.eng.fl_str_mv |
Evaluation of inflammatory, oxidative, and vascular biomarkers in patients with hipercholesterolemia |
title |
Avaliação de biomarcadores inflamatórios, oxidativos e vasculares em pacientes com hipercolesterolemia |
spellingShingle |
Avaliação de biomarcadores inflamatórios, oxidativos e vasculares em pacientes com hipercolesterolemia Pereira, Renata da Silva Hipercolesterolemia Aterosclerose Inflamação Estresse oxidativo Fibrinólise Método automatizado Óxido nítrico Hypercholesterolemia Atherosclerosis Inflammation Oxidative stress Fibrinolysis Automated method Nitric oxide CNPQ::CIENCIAS DA SAUDE::FARMACIA |
title_short |
Avaliação de biomarcadores inflamatórios, oxidativos e vasculares em pacientes com hipercolesterolemia |
title_full |
Avaliação de biomarcadores inflamatórios, oxidativos e vasculares em pacientes com hipercolesterolemia |
title_fullStr |
Avaliação de biomarcadores inflamatórios, oxidativos e vasculares em pacientes com hipercolesterolemia |
title_full_unstemmed |
Avaliação de biomarcadores inflamatórios, oxidativos e vasculares em pacientes com hipercolesterolemia |
title_sort |
Avaliação de biomarcadores inflamatórios, oxidativos e vasculares em pacientes com hipercolesterolemia |
author |
Pereira, Renata da Silva |
author_facet |
Pereira, Renata da Silva |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Moresco, Rafael Noal |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4765801Z9 |
dc.contributor.referee1.fl_str_mv |
Augusti, Paula Rossini |
dc.contributor.referee1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4778049T7 |
dc.contributor.referee2.fl_str_mv |
Brandão, Ricardo |
dc.contributor.referee2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4779496T3 |
dc.contributor.author.fl_str_mv |
Pereira, Renata da Silva |
contributor_str_mv |
Moresco, Rafael Noal Augusti, Paula Rossini Brandão, Ricardo |
dc.subject.por.fl_str_mv |
Hipercolesterolemia Aterosclerose Inflamação Estresse oxidativo Fibrinólise Método automatizado Óxido nítrico |
topic |
Hipercolesterolemia Aterosclerose Inflamação Estresse oxidativo Fibrinólise Método automatizado Óxido nítrico Hypercholesterolemia Atherosclerosis Inflammation Oxidative stress Fibrinolysis Automated method Nitric oxide CNPQ::CIENCIAS DA SAUDE::FARMACIA |
dc.subject.eng.fl_str_mv |
Hypercholesterolemia Atherosclerosis Inflammation Oxidative stress Fibrinolysis Automated method Nitric oxide |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS DA SAUDE::FARMACIA |
description |
Hypercholesterolemia is a determining factor for the development of atherosclerosis, which is considered the main cause of cardiovascular diseases. In Brazil, as in most developed countries, cardiovascular diseases are the leading cause of death. Several mechanisms are involved in the development of atheromatous plaque thus the purpose of this study was to evaluate the association between hypercholesterolemia and inflammatory, vascular, and oxidative biomarkers as well as to develop and validate an analytical method for the automated measurement of nitrite, a metabolite of the oxide oxide (NO) in plasma samples. In the first phase of this study an analytical method for automated measurement of plasma nitrite was developed and validated. This method was precise (r2=0,9998, P<0,001), linear, simple, inexpensive, and applicable to routine monitoring. In phase 2, inflammatory, vascular and oxidative markers were assessed. The groups were: group with hypercholesterolemia (LDL-C ≥ 160 mg / dl) and normocholesterolemic group (LDL-C ≤ 130 mg / dL). Results showed that levels of total cholesterol, LDL-C, HDL-C, triglycerides, apolipoprotein A, apolipoprotein B, advanced oxidation protein products (AOPP), interleukin-6 (IL-6) and D-dimer were significantly higher in hypercholesterolemic patients, while levels of thiol grouping (-SH) and nitrate / nitrite (NOx) were lower in this group. There were also significant correlations for LDL-C and IL-6 (r = 0.693, P <0.0001), LDL-C and NOx (r = -0.314, P <0.05) and LDL-C and - SH (r = -0.327, P <0.05). Thus, hypercholesterolemia promotes increased oxidative stress, inflammation, and fibrinolysis in atherosclerosis. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011-03-21 |
dc.date.accessioned.fl_str_mv |
2013-05-15 |
dc.date.available.fl_str_mv |
2013-05-15 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
PEREIRA, Renata da Silva. EVALUATION OF INFLAMMATORY, OXIDATIVE, AND VASCULAR BIOMARKERS IN PATIENTS WITH HIPERCHOLESTEROLEMIA. 2011. 70 f. Dissertação (Mestrado em Farmacologia) - Universidade Federal de Santa Maria, Santa Maria, 2011. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/5930 |
identifier_str_mv |
PEREIRA, Renata da Silva. EVALUATION OF INFLAMMATORY, OXIDATIVE, AND VASCULAR BIOMARKERS IN PATIENTS WITH HIPERCHOLESTEROLEMIA. 2011. 70 f. Dissertação (Mestrado em Farmacologia) - Universidade Federal de Santa Maria, Santa Maria, 2011. |
url |
http://repositorio.ufsm.br/handle/1/5930 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.cnpq.fl_str_mv |
201000000000 |
dc.relation.confidence.fl_str_mv |
400 500 300 500 500 |
dc.relation.authority.fl_str_mv |
7a69f2ef-75ae-4e01-b1d1-8295e8c94f97 964d0fcb-db67-4cef-977b-ae0d717b866c f181ae30-4746-410b-acb9-e5e5c764a340 e6617091-34a2-401c-87dc-5d8877667a00 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Ciências Farmacêuticas |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
BR |
dc.publisher.department.fl_str_mv |
Análises Clínicas e Toxicológicas |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
collection |
Manancial - Repositório Digital da UFSM |
bitstream.url.fl_str_mv |
http://repositorio.ufsm.br/bitstream/1/5930/1/PEREIRA%2c%20RENATA%20DA%20SILVA.pdf http://repositorio.ufsm.br/bitstream/1/5930/2/PEREIRA%2c%20RENATA%20DA%20SILVA.pdf.txt http://repositorio.ufsm.br/bitstream/1/5930/3/PEREIRA%2c%20RENATA%20DA%20SILVA.pdf.jpg |
bitstream.checksum.fl_str_mv |
038907b63d781ba889e682fb5fc09626 869941ac0df27d52c606220f0fab7da6 334ab4ae535964f8fd39e1cf139fa076 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
|
_version_ |
1794524463453175808 |