Efeitos da VD3 sobre a memória e sobre o sistema purinérgico e colinérgico em um modelo de demência esporádica do tipo Alzheimer em ratos
Ano de defesa: | 2014 |
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Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Santa Maria
|
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica
|
Departamento: |
Bioquímica
|
País: |
BR
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.ufsm.br/handle/1/11244 |
Resumo: | Alzheimer's disease (AD) is considered the main cause of dementia in elderly people worldwide. This disease affects the central nervous system (CNS), especially the cerebral cortex and hippocampus, areas that are responsible for the processes of learning and memory, for this the search for new therapies to treat this condition is constant. Studies indicate that Vitamin D3 (VD3) may be involved in the functions of neurotransmission, neuroprotection and neuroimmunomodulation in different brain processes, in addition to being involved in the homeostasis of calcium (Ca2+). Thus, the aim of this study was to investigate the effects of VD3 on memory and in the activity of enzymes AChE, NTPDase and 5'-Nucleotidase in the hippocampus of rats submitted to a model of sporadic dementia of the Alzheimer type (DETA) in rats. For this, a total of 40 male Wistar rats were used, weighing between 350-400g were divided into 8 groups: control, Vitamin D3 12.5 μg/kg, Vitamin D3 42 μg/kg, Vitamin D3 125 μg/kg streptozotocin (STZ), streptozotocin + Vitamin D3 12.5 μg/kg streptozotocin + Vitamin D3 42 μg/kg streptozotocin + Vitamin D3 125 μg/kg. The animals were anesthetized with ketamine and xylazine (0.5 mg/kg) and received a bilateral injection of streptozotocin (3mg/kg) intracerebroventicular (icv). After surgery the animals was submitted a recovery period for 72 hours. Then the animals were treated with VD3 orally for 21 days. Behavioral tests of open field and object recognition were realized from 21st to 24th day after surgery. After the 24th day the animals were anesthetized with isoflurane and euthanized. In relation to VD3 levels in serum, a significant increase (P<0.05) was observed in the groups that received the highest doses. A memory deficits in STZ group has been found, however, the treatment with VD3 shown to be effective in the preventing of loss of memory (P<0.05). Regarding the AChE activity was found increased in the hippocampus in STZ group, and this increase was attenuated by administration of VD3 (P<0.05). Animals of group with Dementia also showed a reduction in ATP hydrolysis and VD3 was able to prevent this effect (P<0.05). Furthermore, VD3 was able to reverse the increase in the hydrolysis to ADP and the decrease in AMP hydrolysis by STZ-icv. Thus, this study showed that administration of VD3 is capable of maintaining cholinergic neurotransmission, the homeostasis of the purinergic system as well as improve memory in animals with SDAT. |
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2015-11-122015-11-122014-06-13RODRIGUES, Marília Valvassori. EFFECTS OF VD3 ON MEMORY AND ON PURINERGIC AND CHOLINERGIC SYSTEM IN A MODEL OF SPORADIC DEMENTIA OF ALZHEIMER S TYPE IN RATS. 2014. 91 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2014.http://repositorio.ufsm.br/handle/1/11244Alzheimer's disease (AD) is considered the main cause of dementia in elderly people worldwide. This disease affects the central nervous system (CNS), especially the cerebral cortex and hippocampus, areas that are responsible for the processes of learning and memory, for this the search for new therapies to treat this condition is constant. Studies indicate that Vitamin D3 (VD3) may be involved in the functions of neurotransmission, neuroprotection and neuroimmunomodulation in different brain processes, in addition to being involved in the homeostasis of calcium (Ca2+). Thus, the aim of this study was to investigate the effects of VD3 on memory and in the activity of enzymes AChE, NTPDase and 5'-Nucleotidase in the hippocampus of rats submitted to a model of sporadic dementia of the Alzheimer type (DETA) in rats. For this, a total of 40 male Wistar rats were used, weighing between 350-400g were divided into 8 groups: control, Vitamin D3 12.5 μg/kg, Vitamin D3 42 μg/kg, Vitamin D3 125 μg/kg streptozotocin (STZ), streptozotocin + Vitamin D3 12.5 μg/kg streptozotocin + Vitamin D3 42 μg/kg streptozotocin + Vitamin D3 125 μg/kg. The animals were anesthetized with ketamine and xylazine (0.5 mg/kg) and received a bilateral injection of streptozotocin (3mg/kg) intracerebroventicular (icv). After surgery the animals was submitted a recovery period for 72 hours. Then the animals were treated with VD3 orally for 21 days. Behavioral tests of open field and object recognition were realized from 21st to 24th day after surgery. After the 24th day the animals were anesthetized with isoflurane and euthanized. In relation to VD3 levels in serum, a significant increase (P<0.05) was observed in the groups that received the highest doses. A memory deficits in STZ group has been found, however, the treatment with VD3 shown to be effective in the preventing of loss of memory (P<0.05). Regarding the AChE activity was found increased in the hippocampus in STZ group, and this increase was attenuated by administration of VD3 (P<0.05). Animals of group with Dementia also showed a reduction in ATP hydrolysis and VD3 was able to prevent this effect (P<0.05). Furthermore, VD3 was able to reverse the increase in the hydrolysis to ADP and the decrease in AMP hydrolysis by STZ-icv. Thus, this study showed that administration of VD3 is capable of maintaining cholinergic neurotransmission, the homeostasis of the purinergic system as well as improve memory in animals with SDAT.A Doença de Alzheimer (DA) é considerada a principal causa de demência em idosos no mundo. Essa doença afeta o Sistema Nervoso Central (SNC), principalmente o córtex cerebral e o hipocampo, que são áreas responsáveis pelos processos de aprendizagem e memória, por isso a busca por novas terapias para o tratamento dessa patologia é constante. Estudos indicam que a Vitamina D3 (VD3) pode estar envolvida nas funções de neurotransmissão, neuroproteção, neuroimunomodulação e diversos processos cerebrais, além de estar envolvida na homeostase do cálcio (Ca2+). Sendo assim, o objetivo deste estudo foi investigar os efeitos da VD3 sobre a memória e na atividade das enzimas AChE, NTPDase e 5 Nucleotidase em hipocampo de ratos submetidos a um modelo de Demência Esporádica do Tipo Alzheimer (DETA) em ratos. Para isso, foram utilizados um total de 40 ratos machos Wistar, pesando entre 350- 400g, divididos em 8 grupos: controle, Vitamina D3 12,5 μg/kg, Vitamina D3 42 μg/kg, Vitamina D3 125 μg/kg, estreptozotocina (STZ), estreptozotocina + Vitamina D3 12,5 μg/kg, estreptozotocina + Vitamina D3 42 μg/kg, estreptozotocina + Vitamina D3 125 μg/kg. Os animais foram anestesiados com Ketamina e Xilazina (0.5 mg/kg) e receberam uma injeção bilateral de estreptozotocina (3mg/kg) intracerebroventicular (icv). Após o procedimento cirúrgico os animais passaram por um período de recuperação de 72 horas. Em seguida os animais foram tratados com VD3, via oral, durante 21 dias. Os testes comportamentais de campo aberto e reconhecimento de objetos foram realizados do 21º ao 24º dia após a cirurgia. Logo após o 24º dia os animais foram anestesiados com isoflurano e eutanasiados. Em relação os níveis de VD3 em soro, foi observado um aumento significativo (P<0.05) nos grupos que receberam as maiores doses. Um déficit de memória foi encontrado no grupo STZ, no entanto, o tratamento com VD3 mostrou-se eficiente em prevenir esta perda de memória (P<0,05). Quanto à atividade da AChE, foi encontrado um aumento em hipocampo no grupo STZ, sendo que esse aumento foi atenuado pela administração de VD3 (P<0,05). Os animais do grupo com Demência também apresentaram uma redução na hidrólise de ATP e a VD3 foi capaz de prevenir este efeito (P<0,05). Além disso, a VD3 foi capaz de reverter o aumento na hidrólise de ADP e a diminuição da hidrólise de AMP causada pela injeção icv-STZ. Desta forma, este estudo mostrou que a administração de VD3 é capaz de manter a neurotransmissão colinérgica, a homeostase do sistema purinérgico, bem como melhorar a memória em animais com DETA.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em Ciências Biológicas: Bioquímica ToxicológicaUFSMBRBioquímicaDemênciaMemóriaVitamina D3AcetilcolinesteraseE-NTPDaseE-5 - nucleotidaseDementiaMemoryVitamin D3AcetylcholinesteraseE-NTPDaseE-5 - nucleotidaseCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICAEfeitos da VD3 sobre a memória e sobre o sistema purinérgico e colinérgico em um modelo de demência esporádica do tipo Alzheimer em ratosEffects of VD3 on memory and on purinergic and cholinergic system in a model of sporadic dementia of Alzheimer s type in ratsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisBagatini, Margarete Dulcehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4734578J2Spanevello, Roselia Mariahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4702772A1Oliveira, Mauro Schneiderhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4705848A9http://lattes.cnpq.br/0588411415522941Rodrigues, Marília Valvassori200800000002400300300500500a3a1ea30-08b3-4070-b400-48e37ef1cad25b22b329-8e62-473e-8a79-f0e0978437bc2c5b3e7b-5bee-4ab4-92a9-97c6a36565e3cf98ddcb-3930-42cf-9437-be60eebd8056info:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALRODRIGUES, MARILIA VALVASSORI.pdfapplication/pdf1756865http://repositorio.ufsm.br/bitstream/1/11244/1/RODRIGUES%2c%20MARILIA%20VALVASSORI.pdf0d3828eae400cda23536db97fb2e29e8MD51TEXTRODRIGUES, MARILIA VALVASSORI.pdf.txtRODRIGUES, MARILIA VALVASSORI.pdf.txtExtracted texttext/plain126950http://repositorio.ufsm.br/bitstream/1/11244/2/RODRIGUES%2c%20MARILIA%20VALVASSORI.pdf.txt3e95d8ca8fd452e0c2ed8327042aef32MD52THUMBNAILRODRIGUES, MARILIA VALVASSORI.pdf.jpgRODRIGUES, MARILIA VALVASSORI.pdf.jpgIM Thumbnailimage/jpeg5513http://repositorio.ufsm.br/bitstream/1/11244/3/RODRIGUES%2c%20MARILIA%20VALVASSORI.pdf.jpg0ba6dc50bd754f96d45b63dc53193b8eMD531/112442021-01-29 08:23:03.51oai:repositorio.ufsm.br:1/11244Repositório Institucionalhttp://repositorio.ufsm.br/PUBhttp://repositorio.ufsm.br/oai/requestopendoar:39132021-01-29T11:23:03Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Efeitos da VD3 sobre a memória e sobre o sistema purinérgico e colinérgico em um modelo de demência esporádica do tipo Alzheimer em ratos |
dc.title.alternative.eng.fl_str_mv |
Effects of VD3 on memory and on purinergic and cholinergic system in a model of sporadic dementia of Alzheimer s type in rats |
title |
Efeitos da VD3 sobre a memória e sobre o sistema purinérgico e colinérgico em um modelo de demência esporádica do tipo Alzheimer em ratos |
spellingShingle |
Efeitos da VD3 sobre a memória e sobre o sistema purinérgico e colinérgico em um modelo de demência esporádica do tipo Alzheimer em ratos Rodrigues, Marília Valvassori Demência Memória Vitamina D3 Acetilcolinesterase E-NTPDase E-5 - nucleotidase Dementia Memory Vitamin D3 Acetylcholinesterase E-NTPDase E-5 - nucleotidase CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
title_short |
Efeitos da VD3 sobre a memória e sobre o sistema purinérgico e colinérgico em um modelo de demência esporádica do tipo Alzheimer em ratos |
title_full |
Efeitos da VD3 sobre a memória e sobre o sistema purinérgico e colinérgico em um modelo de demência esporádica do tipo Alzheimer em ratos |
title_fullStr |
Efeitos da VD3 sobre a memória e sobre o sistema purinérgico e colinérgico em um modelo de demência esporádica do tipo Alzheimer em ratos |
title_full_unstemmed |
Efeitos da VD3 sobre a memória e sobre o sistema purinérgico e colinérgico em um modelo de demência esporádica do tipo Alzheimer em ratos |
title_sort |
Efeitos da VD3 sobre a memória e sobre o sistema purinérgico e colinérgico em um modelo de demência esporádica do tipo Alzheimer em ratos |
author |
Rodrigues, Marília Valvassori |
author_facet |
Rodrigues, Marília Valvassori |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Bagatini, Margarete Dulce |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4734578J2 |
dc.contributor.referee1.fl_str_mv |
Spanevello, Roselia Maria |
dc.contributor.referee1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4702772A1 |
dc.contributor.referee2.fl_str_mv |
Oliveira, Mauro Schneider |
dc.contributor.referee2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4705848A9 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/0588411415522941 |
dc.contributor.author.fl_str_mv |
Rodrigues, Marília Valvassori |
contributor_str_mv |
Bagatini, Margarete Dulce Spanevello, Roselia Maria Oliveira, Mauro Schneider |
dc.subject.por.fl_str_mv |
Demência Memória Vitamina D3 Acetilcolinesterase E-NTPDase E-5 - nucleotidase |
topic |
Demência Memória Vitamina D3 Acetilcolinesterase E-NTPDase E-5 - nucleotidase Dementia Memory Vitamin D3 Acetylcholinesterase E-NTPDase E-5 - nucleotidase CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
dc.subject.eng.fl_str_mv |
Dementia Memory Vitamin D3 Acetylcholinesterase E-NTPDase E-5 - nucleotidase |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
description |
Alzheimer's disease (AD) is considered the main cause of dementia in elderly people worldwide. This disease affects the central nervous system (CNS), especially the cerebral cortex and hippocampus, areas that are responsible for the processes of learning and memory, for this the search for new therapies to treat this condition is constant. Studies indicate that Vitamin D3 (VD3) may be involved in the functions of neurotransmission, neuroprotection and neuroimmunomodulation in different brain processes, in addition to being involved in the homeostasis of calcium (Ca2+). Thus, the aim of this study was to investigate the effects of VD3 on memory and in the activity of enzymes AChE, NTPDase and 5'-Nucleotidase in the hippocampus of rats submitted to a model of sporadic dementia of the Alzheimer type (DETA) in rats. For this, a total of 40 male Wistar rats were used, weighing between 350-400g were divided into 8 groups: control, Vitamin D3 12.5 μg/kg, Vitamin D3 42 μg/kg, Vitamin D3 125 μg/kg streptozotocin (STZ), streptozotocin + Vitamin D3 12.5 μg/kg streptozotocin + Vitamin D3 42 μg/kg streptozotocin + Vitamin D3 125 μg/kg. The animals were anesthetized with ketamine and xylazine (0.5 mg/kg) and received a bilateral injection of streptozotocin (3mg/kg) intracerebroventicular (icv). After surgery the animals was submitted a recovery period for 72 hours. Then the animals were treated with VD3 orally for 21 days. Behavioral tests of open field and object recognition were realized from 21st to 24th day after surgery. After the 24th day the animals were anesthetized with isoflurane and euthanized. In relation to VD3 levels in serum, a significant increase (P<0.05) was observed in the groups that received the highest doses. A memory deficits in STZ group has been found, however, the treatment with VD3 shown to be effective in the preventing of loss of memory (P<0.05). Regarding the AChE activity was found increased in the hippocampus in STZ group, and this increase was attenuated by administration of VD3 (P<0.05). Animals of group with Dementia also showed a reduction in ATP hydrolysis and VD3 was able to prevent this effect (P<0.05). Furthermore, VD3 was able to reverse the increase in the hydrolysis to ADP and the decrease in AMP hydrolysis by STZ-icv. Thus, this study showed that administration of VD3 is capable of maintaining cholinergic neurotransmission, the homeostasis of the purinergic system as well as improve memory in animals with SDAT. |
publishDate |
2014 |
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2014-06-13 |
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2015-11-12 |
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2015-11-12 |
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info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
RODRIGUES, Marília Valvassori. EFFECTS OF VD3 ON MEMORY AND ON PURINERGIC AND CHOLINERGIC SYSTEM IN A MODEL OF SPORADIC DEMENTIA OF ALZHEIMER S TYPE IN RATS. 2014. 91 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2014. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/11244 |
identifier_str_mv |
RODRIGUES, Marília Valvassori. EFFECTS OF VD3 ON MEMORY AND ON PURINERGIC AND CHOLINERGIC SYSTEM IN A MODEL OF SPORADIC DEMENTIA OF ALZHEIMER S TYPE IN RATS. 2014. 91 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2014. |
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http://repositorio.ufsm.br/handle/1/11244 |
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