Caracterização antigênica de cepas de Moraxella bovis, Moraxella bovoculi e Moraxella ovis com potencial uso vacinal
Ano de defesa: | 2016 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Santa Maria
Centro de Ciências Rurais |
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Medicina Veterinária
|
Departamento: |
Medicina Veterinária
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.ufsm.br/handle/1/11282 |
Resumo: | Infectious bovine keratoconjunctivitis (IBK) is the main ocular disease of cattle. Highly contagious, it is responsible for significant economic losses of livestock worldwide. Moraxella bovis is recognized as the primary agent of the disease in cattle. However, the occurrence of other similar species, including M. ovis and M. bovoculi, recently described, have been common in outbreaks of the disease and are suspected to be causally related to the limited success of preventive measures, especially the use of bacterins containing only strains of M. bovis. This dissertation describes the antigenic characterization of M. bovis, M. bovoculi and M. ovis strains and a commercial vaccine by cross-reactivity using flow cytometry analysis and identification of immunodominant conserved antigens by Western blotting. Antisera against strains of the three species were obtained from immunization of New Zealand rabbits and challenged before a panel of strains isolated from cattle and sheep clinically affected by the disease between 1983 and 2013 as well as reference strains of each species. Field strains of M. bovoculi (Mbv2 and Mbv3) recognized satisfactorily all heterologous strains. However, Mbv3 (M. bovoculi), Mov2 (M. ovis) and Mov3 (M. ovis) strains stood out as the most intense recognition strains of their respective species and suggest that species-specific antigens play an important role in the host immune response. The association of reactivity percentage with the antigenic profiles, evidenced by Western blotting analysis, indicates that the immune response induced by Moraxella spp. appears to be mediated by multiple surface antigens of which many are shared between the three species. Among 32 different proteins identified, 22 (68.7%) were recognized by at least one antiserum in the protein extracts of all strains analyzed by Western blotting. Our study suggests (1) the selection and combination of M. bovis, M. bovoculi and M. ovis strains to be used in the composition of antigenic unit vaccines as IBK control strategy and (2) the use of flow cytometry as the most appropriate methodology for this selection. |
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2017-07-31T16:06:09Z2017-07-31T16:06:09Z2016-09-13http://repositorio.ufsm.br/handle/1/11282Infectious bovine keratoconjunctivitis (IBK) is the main ocular disease of cattle. Highly contagious, it is responsible for significant economic losses of livestock worldwide. Moraxella bovis is recognized as the primary agent of the disease in cattle. However, the occurrence of other similar species, including M. ovis and M. bovoculi, recently described, have been common in outbreaks of the disease and are suspected to be causally related to the limited success of preventive measures, especially the use of bacterins containing only strains of M. bovis. This dissertation describes the antigenic characterization of M. bovis, M. bovoculi and M. ovis strains and a commercial vaccine by cross-reactivity using flow cytometry analysis and identification of immunodominant conserved antigens by Western blotting. Antisera against strains of the three species were obtained from immunization of New Zealand rabbits and challenged before a panel of strains isolated from cattle and sheep clinically affected by the disease between 1983 and 2013 as well as reference strains of each species. Field strains of M. bovoculi (Mbv2 and Mbv3) recognized satisfactorily all heterologous strains. However, Mbv3 (M. bovoculi), Mov2 (M. ovis) and Mov3 (M. ovis) strains stood out as the most intense recognition strains of their respective species and suggest that species-specific antigens play an important role in the host immune response. The association of reactivity percentage with the antigenic profiles, evidenced by Western blotting analysis, indicates that the immune response induced by Moraxella spp. appears to be mediated by multiple surface antigens of which many are shared between the three species. Among 32 different proteins identified, 22 (68.7%) were recognized by at least one antiserum in the protein extracts of all strains analyzed by Western blotting. Our study suggests (1) the selection and combination of M. bovis, M. bovoculi and M. ovis strains to be used in the composition of antigenic unit vaccines as IBK control strategy and (2) the use of flow cytometry as the most appropriate methodology for this selection.Ceratoconjuntivite infecciosa bovina (CIB) é a principal doença ocular de bovinos. Altamente contagiosa, é responsável por significativas perdas econômicas para a pecuária no mundo inteiro. Moraxella bovis é, reconhecidamente, o agente primário da enfermidade em bovinos. Contudo, a ocorrência de outras espécies do gênero, incluindo M. ovis e M. bovoculi, recentemente descrita, têm sido comuns em surtos da doença e são suspeitas de serem causalmente associadas ao sucesso limitado de medidas preventivas, sobretudo do uso de bacterinas contendo apenas cepas de M. bovis. Esta dissertação descreve a caracterização antigênica de cepas de M. bovis, M. bovoculi e M. ovis e de uma vacina comercial através da análise de reatividade cruzada por citometria de fluxo e da identificação de antígenos imunodominantes e conservados através de Western blotting. Antissoros contra cepas das três espécies foram obtidos a partir da imunização de coelhos Nova Zelândia e desafiados frente a um painel de cepas isoladas de bovinos e ovinos clinicamente acometidos pela doença entre 1983 e 2013 assim como cepas de referência de cada espécie. Cepas de campo de M. bovoculi (Mbv2 e Mbv3) reconheceram satisfatoriamente todas as cepas heterólogas. Contudo, as cepas Mbv3 (M. bovoculi), Mov2 (M. ovis) e Mov3 (M. ovis) destacaram-se pela maior intensidade de reconhecimento de cepas de suas respectivas espécies e sugerem que antígenos espécie-específicos desempenham importante papel na resposta imune do hospedeiro. A associação dos percentuais de reatividade aos perfis antigênicos evidenciados através da análise por western blotting indica que a resposta imune induzida por Moraxella spp. parece ser mediada por múltiplos antígenos de superfície dos quais, diversos são compartilhados entre as três espécies. Entre 32 diferentes proteínas detectadas, 22 (68,7%) foram reconhecidas por pelo menos um antissoro nos extratos protéicos de todas as cepas analisadas por western blotting. Nosso estudo sugere a seleção e combinação de cepas de M. bovis, M. bovoculi e M. ovis circulantes para composição da unidade antigênica de vacinas como estratégia de controle de IBK e a utilização de citometria de fluxo como metodologia mais adequada para esta seleção.porUniversidade Federal de Santa MariaCentro de Ciências RuraisPrograma de Pós-Graduação em Medicina VeterináriaUFSMBrasilMedicina VeterináriaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessPinkeyeCitometria de fluxoReatividade cruzadaMoraxella sp.CIBPinkeyeFlow cytometryCross-reactivityMoraxella spIBKCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIACaracterização antigênica de cepas de Moraxella bovis, Moraxella bovoculi e Moraxella ovis com potencial uso vacinalAntigenic characterization of Moraxella bovis, Moraxella bovoculi and Moraxella ovis strains with potential use in vaccinesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisVargas, Agueda Palmira Castagna dehttp://lattes.cnpq.br/1383126157031968Scherer, Charles Ferando Capinoshttp://lattes.cnpq.br/1396090596936396Frandoloso, Frandolosohttp://lattes.cnpq.br/2502891354017410http://lattes.cnpq.br/0800999372009907Kowalski, Ananda Paula50050000000760086704c10-1997-4b84-868c-5547143be87b7ec33c06-2c43-4eb6-978d-d6b58e3b1b1d30897278-c5e7-4146-b3be-7083f7c6fef2d5663dc0-bd95-4390-8ce4-ebb64c664094reponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALKowalski, Ananda Paula.pdfKowalski, Ananda Paula.pdfDissertação de Mestradoapplication/pdf1211677http://repositorio.ufsm.br/bitstream/1/11282/1/Kowalski%2c%20Ananda%20Paula.pdfb6361dc13ab236d9002b5afbfa0c57cdMD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; 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dc.title.por.fl_str_mv |
Caracterização antigênica de cepas de Moraxella bovis, Moraxella bovoculi e Moraxella ovis com potencial uso vacinal |
dc.title.alternative.eng.fl_str_mv |
Antigenic characterization of Moraxella bovis, Moraxella bovoculi and Moraxella ovis strains with potential use in vaccines |
title |
Caracterização antigênica de cepas de Moraxella bovis, Moraxella bovoculi e Moraxella ovis com potencial uso vacinal |
spellingShingle |
Caracterização antigênica de cepas de Moraxella bovis, Moraxella bovoculi e Moraxella ovis com potencial uso vacinal Kowalski, Ananda Paula Pinkeye Citometria de fluxo Reatividade cruzada Moraxella sp. CIB Pinkeye Flow cytometry Cross-reactivity Moraxella sp IBK CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Caracterização antigênica de cepas de Moraxella bovis, Moraxella bovoculi e Moraxella ovis com potencial uso vacinal |
title_full |
Caracterização antigênica de cepas de Moraxella bovis, Moraxella bovoculi e Moraxella ovis com potencial uso vacinal |
title_fullStr |
Caracterização antigênica de cepas de Moraxella bovis, Moraxella bovoculi e Moraxella ovis com potencial uso vacinal |
title_full_unstemmed |
Caracterização antigênica de cepas de Moraxella bovis, Moraxella bovoculi e Moraxella ovis com potencial uso vacinal |
title_sort |
Caracterização antigênica de cepas de Moraxella bovis, Moraxella bovoculi e Moraxella ovis com potencial uso vacinal |
author |
Kowalski, Ananda Paula |
author_facet |
Kowalski, Ananda Paula |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Vargas, Agueda Palmira Castagna de |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/1383126157031968 |
dc.contributor.referee1.fl_str_mv |
Scherer, Charles Ferando Capinos |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/1396090596936396 |
dc.contributor.referee2.fl_str_mv |
Frandoloso, Frandoloso |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/2502891354017410 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/0800999372009907 |
dc.contributor.author.fl_str_mv |
Kowalski, Ananda Paula |
contributor_str_mv |
Vargas, Agueda Palmira Castagna de Scherer, Charles Ferando Capinos Frandoloso, Frandoloso |
dc.subject.por.fl_str_mv |
Pinkeye Citometria de fluxo Reatividade cruzada Moraxella sp. CIB |
topic |
Pinkeye Citometria de fluxo Reatividade cruzada Moraxella sp. CIB Pinkeye Flow cytometry Cross-reactivity Moraxella sp IBK CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
dc.subject.eng.fl_str_mv |
Pinkeye Flow cytometry Cross-reactivity Moraxella sp IBK |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
Infectious bovine keratoconjunctivitis (IBK) is the main ocular disease of cattle. Highly contagious, it is responsible for significant economic losses of livestock worldwide. Moraxella bovis is recognized as the primary agent of the disease in cattle. However, the occurrence of other similar species, including M. ovis and M. bovoculi, recently described, have been common in outbreaks of the disease and are suspected to be causally related to the limited success of preventive measures, especially the use of bacterins containing only strains of M. bovis. This dissertation describes the antigenic characterization of M. bovis, M. bovoculi and M. ovis strains and a commercial vaccine by cross-reactivity using flow cytometry analysis and identification of immunodominant conserved antigens by Western blotting. Antisera against strains of the three species were obtained from immunization of New Zealand rabbits and challenged before a panel of strains isolated from cattle and sheep clinically affected by the disease between 1983 and 2013 as well as reference strains of each species. Field strains of M. bovoculi (Mbv2 and Mbv3) recognized satisfactorily all heterologous strains. However, Mbv3 (M. bovoculi), Mov2 (M. ovis) and Mov3 (M. ovis) strains stood out as the most intense recognition strains of their respective species and suggest that species-specific antigens play an important role in the host immune response. The association of reactivity percentage with the antigenic profiles, evidenced by Western blotting analysis, indicates that the immune response induced by Moraxella spp. appears to be mediated by multiple surface antigens of which many are shared between the three species. Among 32 different proteins identified, 22 (68.7%) were recognized by at least one antiserum in the protein extracts of all strains analyzed by Western blotting. Our study suggests (1) the selection and combination of M. bovis, M. bovoculi and M. ovis strains to be used in the composition of antigenic unit vaccines as IBK control strategy and (2) the use of flow cytometry as the most appropriate methodology for this selection. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016-09-13 |
dc.date.accessioned.fl_str_mv |
2017-07-31T16:06:09Z |
dc.date.available.fl_str_mv |
2017-07-31T16:06:09Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/11282 |
url |
http://repositorio.ufsm.br/handle/1/11282 |
dc.language.iso.fl_str_mv |
por |
language |
por |
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500500000007 |
dc.relation.confidence.fl_str_mv |
600 |
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86704c10-1997-4b84-868c-5547143be87b 7ec33c06-2c43-4eb6-978d-d6b58e3b1b1d 30897278-c5e7-4146-b3be-7083f7c6fef2 d5663dc0-bd95-4390-8ce4-ebb64c664094 |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Rurais |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Medicina Veterinária |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Medicina Veterinária |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Rurais |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
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Universidade Federal de Santa Maria (UFSM) |
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UFSM |
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Manancial - Repositório Digital da UFSM |
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