Nanoencapsulação de ftalocianina de zinco para o uso em terapia fotodinâmica
Ano de defesa: | 2015 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Santa Maria
Centro de Ciências Naturais e Exatas |
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Química
|
Departamento: |
Química
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.ufsm.br/handle/1/18228 |
Resumo: | The present work aims to investigate the influence of nanoencapsulation of zinc phthalocyanine photosensitizer (ZnPc) in polymeric nanoparticles for use in photodynamic therapy (PDT). Three formulations of ZnPc loaded nanocapsules (ZnPc/NCs), conventional and lipid core, constituted by polymers chitosan (NCA), poly-ε-caprolactone (NCB), and poly-ε-caprolactone coated with chitosan (NCC) were prepared by the interfacial deposition of preformed polymer method. The physicochemical characterization of ZnPc/NCs was performed (hydrodynamic radius, polydispersity index, zeta potential, pH, encapsulation efficiency, morphology and stability indicative evaluation at different temperatures over time). Photophysical (ZnPc aggregation) and photochemical studies (photodegradation of 1.3-diphenylisobenzofuran, DPBF, of ZnPc, and calculation of singlet oxygen quantum yield, Δ) were carried out and an evaluation the in vitro release kinetics study was also be done. The formulations presented physicochemical characteristics suitable to our expectations, with particle size in the nanometer scale (119 nm ± 3.86, 95 nm ± 0.82 and 83 nm ± 0.54 to NCA, NCB and NCC, respectively), index polydispersity lower than 0.24. Regarding the zeta potential, the nanocapsules showed positive and moderately high value for NCA and NCC (+34 mV ± 3.65 and +22 mV ± 0.46, respectively) and a negative one for NCB (-13 mV ± 0.80) and all formulations showed high encapsulation efficiency (greater than 99%). The ZnPc/NCs showed spherical shape and were stable during the storage time (90 days) at temperatures of 25 °C and 37 °C. Regarding the photophysical and photochemical studies, the free ZnPc (unencapsulated) did not suffer aggregation in the concentrations and solvents studied, and the ZnPc/NCs efficiency for the production of 1O2 was been confirmed by the high values found for Δ (0.61 ± 0.13, 0 36 ± 0.04, 0.41 ± 0.02 for NCA, NCB and NCC, respectively). In the time scale usually used in PDT (150 s) the photosensitizer shows photostability and only a small photodegradation was been observed when a high dose of light was applied. The release kinetics study of ZnPc loaded nanocapsules showed controlled release and no burst effect. The release profile follows the Higuchi's kinetic model for nanocapsules indicating that the process is controlled by diffusion, whereas the release depends on the system composition and increases in the order: NCC <NCB <NCA. Based on the results obtained in this work, the conventional and lipid core nanocapsules containing ZnPc are promising delivery systems for use in PDT. |
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2019-09-13T19:58:56Z2019-09-13T19:58:56Z2015-02-26http://repositorio.ufsm.br/handle/1/18228The present work aims to investigate the influence of nanoencapsulation of zinc phthalocyanine photosensitizer (ZnPc) in polymeric nanoparticles for use in photodynamic therapy (PDT). Three formulations of ZnPc loaded nanocapsules (ZnPc/NCs), conventional and lipid core, constituted by polymers chitosan (NCA), poly-ε-caprolactone (NCB), and poly-ε-caprolactone coated with chitosan (NCC) were prepared by the interfacial deposition of preformed polymer method. The physicochemical characterization of ZnPc/NCs was performed (hydrodynamic radius, polydispersity index, zeta potential, pH, encapsulation efficiency, morphology and stability indicative evaluation at different temperatures over time). Photophysical (ZnPc aggregation) and photochemical studies (photodegradation of 1.3-diphenylisobenzofuran, DPBF, of ZnPc, and calculation of singlet oxygen quantum yield, Δ) were carried out and an evaluation the in vitro release kinetics study was also be done. The formulations presented physicochemical characteristics suitable to our expectations, with particle size in the nanometer scale (119 nm ± 3.86, 95 nm ± 0.82 and 83 nm ± 0.54 to NCA, NCB and NCC, respectively), index polydispersity lower than 0.24. Regarding the zeta potential, the nanocapsules showed positive and moderately high value for NCA and NCC (+34 mV ± 3.65 and +22 mV ± 0.46, respectively) and a negative one for NCB (-13 mV ± 0.80) and all formulations showed high encapsulation efficiency (greater than 99%). The ZnPc/NCs showed spherical shape and were stable during the storage time (90 days) at temperatures of 25 °C and 37 °C. Regarding the photophysical and photochemical studies, the free ZnPc (unencapsulated) did not suffer aggregation in the concentrations and solvents studied, and the ZnPc/NCs efficiency for the production of 1O2 was been confirmed by the high values found for Δ (0.61 ± 0.13, 0 36 ± 0.04, 0.41 ± 0.02 for NCA, NCB and NCC, respectively). In the time scale usually used in PDT (150 s) the photosensitizer shows photostability and only a small photodegradation was been observed when a high dose of light was applied. The release kinetics study of ZnPc loaded nanocapsules showed controlled release and no burst effect. The release profile follows the Higuchi's kinetic model for nanocapsules indicating that the process is controlled by diffusion, whereas the release depends on the system composition and increases in the order: NCC <NCB <NCA. Based on the results obtained in this work, the conventional and lipid core nanocapsules containing ZnPc are promising delivery systems for use in PDT.O presente trabalho tem por objetivo investigar a influência da nanoencapsulação do fotossensibilizador ftalocianina de zinco (ZnPc) em nanopartículas poliméricas para uso em terapia fotodinâmica (TFD). Três formulações de nanocápsulas contendo ZnPc (ZnPc/NCs), convencional e de núcleo lipídico, constituídas pelos polímeros quitosana (NCA), poli-ε-caprolactona (NCB) e poli-ε-caprolactona recoberto com quitosana (NCC) foram preparadas pelo método de deposição interfacial do polímero pré-formado. A caracterização físico-química foi realizada (raio hidrodinâmico, índice de polidispersão, potencial zeta, pH, eficiência de encapsulamento, avaliação da morfologia e indicativos de estabilidade em diferentes temperaturas ao longo do tempo) das ZnPc/NCs. Além disso, foram realizados estudos fotofísico (teste de agregação da ZnPc) e fotoquímicos (fotodegradação do 1,3-difenilisobenzofurano, DPBF, da ZnPc, e cálculo do rendimento quântico do oxigênio singlete, Δ) e avaliou-se o estudo da cinética de liberação in vitro. As formulações apresentaram características físico-químicas adequadas à nossa expectativa, com tamanho de partícula na escala nanométrica (119 nm ± 3,86, 95 nm ± 0,82 e 83 nm ± 0,54, para NCA, NCB e NCC, respectivamente) e índice de polidispersão inferiores 0,24. Em relação ao potencial zeta, as nanocápsulas apresentaram valores positivos e moderadamente elevados para NCA e NCC (+34 mV ± 3,65 e +22 mV ± 0,46, respectivamente) e negativo para a NCB (-13 mV ± 0,80), e todas as formulações apresentaram alta eficiência de encapsulamento (superior a 99 %). As ZnPc/NCs apresentaram forma esférica e mantiveram-se estáveis durante o tempo de armazenamento (90 dias) nas temperaturas de 25 ºC e 37 ºC. Em relação aos estudos fotofísico e fotoquímicos, a ZnPc livre (não encapsulada) não sofreu agregação nas concentrações e solventes estudados, e as ZnPc/NCs foram eficientes para produção de 1O2, o qual foi comprovado pelos altos valores de Δ encontrados (0,61 0,13, 0,36 0,04, 0,41 0,02 para NCA, NCB e NCC, respectivamente). Na escala de tempo usualmente utilizada em TFD (150 s), o fotossensibilizador apresentou-se fotoestável e foi observada apenas uma pequena fotodegradação quando uma dose elevada de luz foi aplicada. O estudo da cinética de liberação da ZnPc contida nas nanocápsulas apresentou uma liberação controlada e sem efeito burst. O perfil de liberação segue o modelo cinético de Higuchi para as nanocápsulas indicando que o processo é controlado por difusão, sendo que a liberação depende da composição do sistema e aumenta na ordem: NCC < NCB < NCA. Baseado nos resultados obtidos neste trabalho, as nanocápsulas convencional e de núcleo lipídico contendo ZnPc são sistemas de liberação promissores para emprego na TFD.Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPqporUniversidade Federal de Santa MariaCentro de Ciências Naturais e ExatasPrograma de Pós-Graduação em QuímicaUFSMBrasilQuímicaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessFtalocianina de zinco (ZnPc)Nanopartículas poliméricasQuitosanaPoli-ε-caprolactona (PCL)Terapia fotodinâmica (TFD)Zinc phthalocyanine (ZnPc)Polymeric nanoparticlesChitosanPoly-ε-caprolactone (PCL)Photodynamic therapy (PDT)CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICANanoencapsulação de ftalocianina de zinco para o uso em terapia fotodinâmicaZinc phthalocyanine nanocapsulation for use in photodynamic therapyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisVilletti, Marcos Antoniohttp://lattes.cnpq.br/8504489050993642Santos, Roberto Christ Viannahttp://lattes.cnpq.br/9176719594431835Ourique, Aline Ferreirahttp://lattes.cnpq.br/7478810804464054http://lattes.cnpq.br/2989128901697289Souza, Thiane Deprá de100600000000600606f8835-e936-4079-89b9-60e12681def4bd507363-ba38-4973-a8ff-5f2de435e57ba269af39-b3ff-4399-b1b5-aa61351e6aafe2d93dc4-ade2-49e7-a69e-988e5ab8f78dreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMCC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; 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dc.title.por.fl_str_mv |
Nanoencapsulação de ftalocianina de zinco para o uso em terapia fotodinâmica |
dc.title.alternative.eng.fl_str_mv |
Zinc phthalocyanine nanocapsulation for use in photodynamic therapy |
title |
Nanoencapsulação de ftalocianina de zinco para o uso em terapia fotodinâmica |
spellingShingle |
Nanoencapsulação de ftalocianina de zinco para o uso em terapia fotodinâmica Souza, Thiane Deprá de Ftalocianina de zinco (ZnPc) Nanopartículas poliméricas Quitosana Poli-ε-caprolactona (PCL) Terapia fotodinâmica (TFD) Zinc phthalocyanine (ZnPc) Polymeric nanoparticles Chitosan Poly-ε-caprolactone (PCL) Photodynamic therapy (PDT) CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA |
title_short |
Nanoencapsulação de ftalocianina de zinco para o uso em terapia fotodinâmica |
title_full |
Nanoencapsulação de ftalocianina de zinco para o uso em terapia fotodinâmica |
title_fullStr |
Nanoencapsulação de ftalocianina de zinco para o uso em terapia fotodinâmica |
title_full_unstemmed |
Nanoencapsulação de ftalocianina de zinco para o uso em terapia fotodinâmica |
title_sort |
Nanoencapsulação de ftalocianina de zinco para o uso em terapia fotodinâmica |
author |
Souza, Thiane Deprá de |
author_facet |
Souza, Thiane Deprá de |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Villetti, Marcos Antonio |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/8504489050993642 |
dc.contributor.referee1.fl_str_mv |
Santos, Roberto Christ Vianna |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/9176719594431835 |
dc.contributor.referee2.fl_str_mv |
Ourique, Aline Ferreira |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/7478810804464054 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/2989128901697289 |
dc.contributor.author.fl_str_mv |
Souza, Thiane Deprá de |
contributor_str_mv |
Villetti, Marcos Antonio Santos, Roberto Christ Vianna Ourique, Aline Ferreira |
dc.subject.por.fl_str_mv |
Ftalocianina de zinco (ZnPc) Nanopartículas poliméricas Quitosana Poli-ε-caprolactona (PCL) Terapia fotodinâmica (TFD) |
topic |
Ftalocianina de zinco (ZnPc) Nanopartículas poliméricas Quitosana Poli-ε-caprolactona (PCL) Terapia fotodinâmica (TFD) Zinc phthalocyanine (ZnPc) Polymeric nanoparticles Chitosan Poly-ε-caprolactone (PCL) Photodynamic therapy (PDT) CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA |
dc.subject.eng.fl_str_mv |
Zinc phthalocyanine (ZnPc) Polymeric nanoparticles Chitosan Poly-ε-caprolactone (PCL) Photodynamic therapy (PDT) |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA |
description |
The present work aims to investigate the influence of nanoencapsulation of zinc phthalocyanine photosensitizer (ZnPc) in polymeric nanoparticles for use in photodynamic therapy (PDT). Three formulations of ZnPc loaded nanocapsules (ZnPc/NCs), conventional and lipid core, constituted by polymers chitosan (NCA), poly-ε-caprolactone (NCB), and poly-ε-caprolactone coated with chitosan (NCC) were prepared by the interfacial deposition of preformed polymer method. The physicochemical characterization of ZnPc/NCs was performed (hydrodynamic radius, polydispersity index, zeta potential, pH, encapsulation efficiency, morphology and stability indicative evaluation at different temperatures over time). Photophysical (ZnPc aggregation) and photochemical studies (photodegradation of 1.3-diphenylisobenzofuran, DPBF, of ZnPc, and calculation of singlet oxygen quantum yield, Δ) were carried out and an evaluation the in vitro release kinetics study was also be done. The formulations presented physicochemical characteristics suitable to our expectations, with particle size in the nanometer scale (119 nm ± 3.86, 95 nm ± 0.82 and 83 nm ± 0.54 to NCA, NCB and NCC, respectively), index polydispersity lower than 0.24. Regarding the zeta potential, the nanocapsules showed positive and moderately high value for NCA and NCC (+34 mV ± 3.65 and +22 mV ± 0.46, respectively) and a negative one for NCB (-13 mV ± 0.80) and all formulations showed high encapsulation efficiency (greater than 99%). The ZnPc/NCs showed spherical shape and were stable during the storage time (90 days) at temperatures of 25 °C and 37 °C. Regarding the photophysical and photochemical studies, the free ZnPc (unencapsulated) did not suffer aggregation in the concentrations and solvents studied, and the ZnPc/NCs efficiency for the production of 1O2 was been confirmed by the high values found for Δ (0.61 ± 0.13, 0 36 ± 0.04, 0.41 ± 0.02 for NCA, NCB and NCC, respectively). In the time scale usually used in PDT (150 s) the photosensitizer shows photostability and only a small photodegradation was been observed when a high dose of light was applied. The release kinetics study of ZnPc loaded nanocapsules showed controlled release and no burst effect. The release profile follows the Higuchi's kinetic model for nanocapsules indicating that the process is controlled by diffusion, whereas the release depends on the system composition and increases in the order: NCC <NCB <NCA. Based on the results obtained in this work, the conventional and lipid core nanocapsules containing ZnPc are promising delivery systems for use in PDT. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015-02-26 |
dc.date.accessioned.fl_str_mv |
2019-09-13T19:58:56Z |
dc.date.available.fl_str_mv |
2019-09-13T19:58:56Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/18228 |
url |
http://repositorio.ufsm.br/handle/1/18228 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.cnpq.fl_str_mv |
100600000000 |
dc.relation.confidence.fl_str_mv |
600 |
dc.relation.authority.fl_str_mv |
606f8835-e936-4079-89b9-60e12681def4 bd507363-ba38-4973-a8ff-5f2de435e57b a269af39-b3ff-4399-b1b5-aa61351e6aaf e2d93dc4-ade2-49e7-a69e-988e5ab8f78d |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Naturais e Exatas |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Química |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Química |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Naturais e Exatas |
dc.source.none.fl_str_mv |
reponame:Manancial - Repositório Digital da UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Manancial - Repositório Digital da UFSM |
collection |
Manancial - Repositório Digital da UFSM |
bitstream.url.fl_str_mv |
http://repositorio.ufsm.br/bitstream/1/18228/2/license_rdf http://repositorio.ufsm.br/bitstream/1/18228/3/license.txt http://repositorio.ufsm.br/bitstream/1/18228/1/DIS_PPGQUIMICA_2015_SOUZA_THIANE.pdf http://repositorio.ufsm.br/bitstream/1/18228/4/DIS_PPGQUIMICA_2015_SOUZA_THIANE.pdf.txt http://repositorio.ufsm.br/bitstream/1/18228/5/DIS_PPGQUIMICA_2015_SOUZA_THIANE.pdf.jpg |
bitstream.checksum.fl_str_mv |
4460e5956bc1d1639be9ae6146a50347 2f0571ecee68693bd5cd3f17c1e075df 5a7a24471dfa5bb18d9d48bd30252cd8 4db3e4030fde63dc2147df48464cbe85 0517a4bbcf5f9c36a0850e1c0e7c982f |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 MD5 |
repository.name.fl_str_mv |
Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
|
_version_ |
1794524290852323328 |