Cianobactérias heterocitadas e euglenas vermelhas em lavoura de arroz irrigado por inundação em planossolo háplico, Santa Maria, RS
Ano de defesa: | 2010 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , , , |
Tipo de documento: | Tese |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Santa Maria
|
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Ciência do Solo
|
Departamento: |
Agronomia
|
País: |
BR
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.ufsm.br/handle/1/3323 |
Resumo: | Oxyreduction reactions resulting from the soil flood promote lighest availability of nutrients from fertilizer application, soil solution. Although the management of drainage, necessary to improve plant establishment contributes to the loss of fertility in these agroecosystems, events such as leaching, runoff and leakage of water from the blocks with the rains keep this ecosystem enriched and pro- development of phytoplankton. Euglenoids and cyanobacteria are favored by these conditions to grow excessively (blooms) and causing environmental impacts that are applicable to the water bodies receiving drainage waters. Moreover, many of cyanobacteria that develop in these environments also contribute to the maintenance of soil fertility. Within this context, with this work, to characterize populations of heterocytous cyanobacterias that contribute to soil fertility and euglenas red blooms forming in agroecosystem of flooded rice. To achieve these objectives, samples of water and soil in three experimental blocks of rice, and conducted two separate studies: culture, isolation and morfomolecular characterization of heterocytous cyanobacteria and cultivation, morphological characterization, monitoring on site and location of blooms cysts of resistance in the soil, forming euglenas pigmented red blooms. Morphological analysis allowed the characterization of four genera of heterocytous cyanobacteria that contribute to soil fertility: Nostoc, Anabaena, Cilindrospermum and Calotrix. Sequencing of 16S RNA gene was only positive for a strain of the genus Nostoc (Nostoc linckia) which, by phylogenetic analysis showed to be a lineage close to Nostoc sp. TH1S01 e Nostoc piscinale. The results obtained in experiments that were aimed at location of the cysts of resistance suggested that such cysts are found at a depth 0-5 cm of soil, however, focuses more on the surface. For euglenoids there was no evidence of the location of the cysts of resistance even at the surface. Monitoring the appearance of blooms of red euglenas shows that the absence of turbulence in water depths, influenced by wind velocity, and water temperature, influenced by atmospheric temperature and solar radiation are key factors for development. It follows from this work, the dynamics of both populations of cyanobacteria as euglenoids in crop irrigated rice is controlled by many biotic and abiotic making knowledge of their diversity and behavior, in practice very difficult having to therefore, the need to employ different methodologies and continuous monitoring and prolonged. It follows further that the use of in vitro culture, while contributing to the research morfomoleculares, promotes populations changes and further complicates the charactrization of the species. |
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2017-04-052017-04-052010-08-30VASCONCELLOS, Noeli Júlia Schussler de. Heterocitic cyanobacteria and red euglenophyceae in flooded rice agroecosystem. 2010. 101 f. Tese (Doutorado em Agronomia) - Universidade Federal de Santa Maria, Santa Maria, 2010.http://repositorio.ufsm.br/handle/1/3323Oxyreduction reactions resulting from the soil flood promote lighest availability of nutrients from fertilizer application, soil solution. Although the management of drainage, necessary to improve plant establishment contributes to the loss of fertility in these agroecosystems, events such as leaching, runoff and leakage of water from the blocks with the rains keep this ecosystem enriched and pro- development of phytoplankton. Euglenoids and cyanobacteria are favored by these conditions to grow excessively (blooms) and causing environmental impacts that are applicable to the water bodies receiving drainage waters. Moreover, many of cyanobacteria that develop in these environments also contribute to the maintenance of soil fertility. Within this context, with this work, to characterize populations of heterocytous cyanobacterias that contribute to soil fertility and euglenas red blooms forming in agroecosystem of flooded rice. To achieve these objectives, samples of water and soil in three experimental blocks of rice, and conducted two separate studies: culture, isolation and morfomolecular characterization of heterocytous cyanobacteria and cultivation, morphological characterization, monitoring on site and location of blooms cysts of resistance in the soil, forming euglenas pigmented red blooms. Morphological analysis allowed the characterization of four genera of heterocytous cyanobacteria that contribute to soil fertility: Nostoc, Anabaena, Cilindrospermum and Calotrix. Sequencing of 16S RNA gene was only positive for a strain of the genus Nostoc (Nostoc linckia) which, by phylogenetic analysis showed to be a lineage close to Nostoc sp. TH1S01 e Nostoc piscinale. The results obtained in experiments that were aimed at location of the cysts of resistance suggested that such cysts are found at a depth 0-5 cm of soil, however, focuses more on the surface. For euglenoids there was no evidence of the location of the cysts of resistance even at the surface. Monitoring the appearance of blooms of red euglenas shows that the absence of turbulence in water depths, influenced by wind velocity, and water temperature, influenced by atmospheric temperature and solar radiation are key factors for development. It follows from this work, the dynamics of both populations of cyanobacteria as euglenoids in crop irrigated rice is controlled by many biotic and abiotic making knowledge of their diversity and behavior, in practice very difficult having to therefore, the need to employ different methodologies and continuous monitoring and prolonged. It follows further that the use of in vitro culture, while contributing to the research morfomoleculares, promotes populations changes and further complicates the charactrization of the species.As reações de oxiredução decorrentes da inundação em lavoura de arroz irrigado promovem uma maior disponibilidade de nutrientes, oriundos da aplicação de fertilizantes, na solução do solo. Embora o manejo de drenagem inicial, necessário para o melhor estabelecimento das plântulas, contribua para a perda de fertilidade nesses agroecossistemas, eventos como a lixiviação, o escorrimento superficial e o extravasamento de água das quadras com as chuvas mantêm esse tipo de ecossistema enriquecido e favorável ao desenvolvimento do fitoplâncton. O campo de arroz irrigado é um ambiente aquático temporário sujeito a grandes variações quanto a isolação, temperatura, pH, concentração do oxigênio dissolvido e estado nutricional devido a perturbações freqüentes através de práticas como o uso de agrotóxicos (WHATANABE e ROGER, 1985). Cianobactérias e euglenofíceas são favorecidas por essas condições crescendo excessivamente (florações) e causando impactos ambientais que são extensivos aos corpos d`água receptores das águas de drenagem. Por outro lado, muitas das cianobactérias que se desenvolvem nesses ambientes também contribuem para a manutenção da fertilidade do solo. Nesse contexto, objetivou-se com esse trabalho, caracterizar populações de cianobactérias heterocitadas que contribuem para a fertilidade do solo e euglenas vermelhas formadoras de florações em agroecossistema de arroz irrigado por inundação. Para atingir esses objetivos, foram coletadas amostras de água e solo em três quadras experimentais de arroz irrigado e conduzido dois estudos distintos: cultivo, isolamento e caracterização morfomolecular de cianobactérias heterocitadas e cultivo, caracterização morfológica, monitoramento in loco das florações e localização dos cistos de resistência no solo, de euglenas pigmentadas formadoras de florações vermelhas. A análise morfológica permitiu a caracterização de quatro gêneros de cianobactérias heterocitadas que contribuem para a fertilidade do solo: Nostoc, Anabaena, Cilindrospermum e Calotrix. O sequenciamento da região 16S do rDNA foi positivo apenas para uma linhagem do gênero Nostoc (Nostoc linckia) que, pela análise filogenética, está no mesmo grupo de Nostoc TH1S01 e Nostoc piscinale. Os resultados obtidos nos experimentos que visaram a localização dos cistos de resistência evidenciaram que estes se encontram numa profundidade de 0 a 5 cm do solo, porém, concentrando-se mais na superfíce. Para as euglenofíceas não houve comprovação da localização dos cistos de resistência nem mesmo na superfície. O monitoramento do surgimento das florações de euglenas vermelhas mostra que a ausência de turbulência na lâmina d`água, influenciada pela velocidade dos ventos, e a temperatura da água, influenciada pela temperatura atmosférica e radiação solar, são fatores-chave para o desenvolvimento. Conclui-se que a dinâmica das populações tanto de cianobactérias como de euglenofíceas em lavoura de arroz irrigado por inundação é comandada por muitos fatores bióticos e abióticos os quais tornam o conhecimento da sua diversidade e comportamento, na prática, muito difícil havendo, para tal, a necessidade de se empregar metodologias variadas e monitoramento continuo e prolongado. Conclui-se, ainda, que o emprego de cultivo in vitro permite a investigação morfomofológica, promove alterações das populações e dificultam ainda mais a identificação das espécies.application/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em Ciência do SoloUFSMBRAgronomiaSolo encharcadoFixação de nitrogênioOryza sativaFlooded soilBiological nitrogen-fixingOryza sativaCNPQ::CIENCIAS AGRARIAS::AGRONOMIA::CIENCIA DO SOLOCianobactérias heterocitadas e euglenas vermelhas em lavoura de arroz irrigado por inundação em planossolo háplico, Santa Maria, RSHeterocitic cyanobacteria and red euglenophyceae in flooded rice agroecosysteminfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisKaminski, Joãohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783777Y9Oliveira, Maria Angelicahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4723571J7Antoniolli, Zaida Inêshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787113T1Silva, Sandra Maria Alves dahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787398U8Golle, Diego Pascoalhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4730400H4http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4799917P3Vasconcellos, Noeli Júlia Schussler de500100100005400500300300500300300d031d511-917c-4dea-afa7-8eea4d8997ec2d15c487-e977-4850-a1f7-b4c6d1a603c20fdde02d-c880-4f54-91d7-eea98df23ea590ef9c57-343d-4322-b0d6-ea7bb2e94214fc50d38e-2d0e-4cd3-8f4b-05b08e51bf309532f06b-366a-46cd-9752-9cd74a9a9225info:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALVASCONCELLOS, NOELI JULIA SCHUSSLER.pdfapplication/pdf8276993http://repositorio.ufsm.br/bitstream/1/3323/1/VASCONCELLOS%2c%20NOELI%20JULIA%20SCHUSSLER.pdf0b591909a0e6c1a0efa4325f47d09459MD51TEXTVASCONCELLOS, NOELI JULIA SCHUSSLER.pdf.txtVASCONCELLOS, NOELI JULIA SCHUSSLER.pdf.txtExtracted texttext/plain190626http://repositorio.ufsm.br/bitstream/1/3323/2/VASCONCELLOS%2c%20NOELI%20JULIA%20SCHUSSLER.pdf.txt6d92c169fec631d080a9c2b4d3d02920MD52THUMBNAILVASCONCELLOS, NOELI JULIA SCHUSSLER.pdf.jpgVASCONCELLOS, NOELI JULIA SCHUSSLER.pdf.jpgIM Thumbnailimage/jpeg5138http://repositorio.ufsm.br/bitstream/1/3323/3/VASCONCELLOS%2c%20NOELI%20JULIA%20SCHUSSLER.pdf.jpg1442b53da2d82cc75ece2881ee01f48bMD531/33232017-07-25 10:54:24.205oai:repositorio.ufsm.br:1/3323Repositório Institucionalhttp://repositorio.ufsm.br/PUBhttp://repositorio.ufsm.br/oai/requestopendoar:39132017-07-25T13:54:24Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Cianobactérias heterocitadas e euglenas vermelhas em lavoura de arroz irrigado por inundação em planossolo háplico, Santa Maria, RS |
dc.title.alternative.eng.fl_str_mv |
Heterocitic cyanobacteria and red euglenophyceae in flooded rice agroecosystem |
title |
Cianobactérias heterocitadas e euglenas vermelhas em lavoura de arroz irrigado por inundação em planossolo háplico, Santa Maria, RS |
spellingShingle |
Cianobactérias heterocitadas e euglenas vermelhas em lavoura de arroz irrigado por inundação em planossolo háplico, Santa Maria, RS Vasconcellos, Noeli Júlia Schussler de Solo encharcado Fixação de nitrogênio Oryza sativa Flooded soil Biological nitrogen-fixing Oryza sativa CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::CIENCIA DO SOLO |
title_short |
Cianobactérias heterocitadas e euglenas vermelhas em lavoura de arroz irrigado por inundação em planossolo háplico, Santa Maria, RS |
title_full |
Cianobactérias heterocitadas e euglenas vermelhas em lavoura de arroz irrigado por inundação em planossolo háplico, Santa Maria, RS |
title_fullStr |
Cianobactérias heterocitadas e euglenas vermelhas em lavoura de arroz irrigado por inundação em planossolo háplico, Santa Maria, RS |
title_full_unstemmed |
Cianobactérias heterocitadas e euglenas vermelhas em lavoura de arroz irrigado por inundação em planossolo háplico, Santa Maria, RS |
title_sort |
Cianobactérias heterocitadas e euglenas vermelhas em lavoura de arroz irrigado por inundação em planossolo háplico, Santa Maria, RS |
author |
Vasconcellos, Noeli Júlia Schussler de |
author_facet |
Vasconcellos, Noeli Júlia Schussler de |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Kaminski, João |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783777Y9 |
dc.contributor.referee1.fl_str_mv |
Oliveira, Maria Angelica |
dc.contributor.referee1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4723571J7 |
dc.contributor.referee2.fl_str_mv |
Antoniolli, Zaida Inês |
dc.contributor.referee2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787113T1 |
dc.contributor.referee3.fl_str_mv |
Silva, Sandra Maria Alves da |
dc.contributor.referee3Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787398U8 |
dc.contributor.referee4.fl_str_mv |
Golle, Diego Pascoal |
dc.contributor.referee4Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4730400H4 |
dc.contributor.authorLattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4799917P3 |
dc.contributor.author.fl_str_mv |
Vasconcellos, Noeli Júlia Schussler de |
contributor_str_mv |
Kaminski, João Oliveira, Maria Angelica Antoniolli, Zaida Inês Silva, Sandra Maria Alves da Golle, Diego Pascoal |
dc.subject.por.fl_str_mv |
Solo encharcado Fixação de nitrogênio Oryza sativa |
topic |
Solo encharcado Fixação de nitrogênio Oryza sativa Flooded soil Biological nitrogen-fixing Oryza sativa CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::CIENCIA DO SOLO |
dc.subject.eng.fl_str_mv |
Flooded soil Biological nitrogen-fixing Oryza sativa |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::CIENCIA DO SOLO |
description |
Oxyreduction reactions resulting from the soil flood promote lighest availability of nutrients from fertilizer application, soil solution. Although the management of drainage, necessary to improve plant establishment contributes to the loss of fertility in these agroecosystems, events such as leaching, runoff and leakage of water from the blocks with the rains keep this ecosystem enriched and pro- development of phytoplankton. Euglenoids and cyanobacteria are favored by these conditions to grow excessively (blooms) and causing environmental impacts that are applicable to the water bodies receiving drainage waters. Moreover, many of cyanobacteria that develop in these environments also contribute to the maintenance of soil fertility. Within this context, with this work, to characterize populations of heterocytous cyanobacterias that contribute to soil fertility and euglenas red blooms forming in agroecosystem of flooded rice. To achieve these objectives, samples of water and soil in three experimental blocks of rice, and conducted two separate studies: culture, isolation and morfomolecular characterization of heterocytous cyanobacteria and cultivation, morphological characterization, monitoring on site and location of blooms cysts of resistance in the soil, forming euglenas pigmented red blooms. Morphological analysis allowed the characterization of four genera of heterocytous cyanobacteria that contribute to soil fertility: Nostoc, Anabaena, Cilindrospermum and Calotrix. Sequencing of 16S RNA gene was only positive for a strain of the genus Nostoc (Nostoc linckia) which, by phylogenetic analysis showed to be a lineage close to Nostoc sp. TH1S01 e Nostoc piscinale. The results obtained in experiments that were aimed at location of the cysts of resistance suggested that such cysts are found at a depth 0-5 cm of soil, however, focuses more on the surface. For euglenoids there was no evidence of the location of the cysts of resistance even at the surface. Monitoring the appearance of blooms of red euglenas shows that the absence of turbulence in water depths, influenced by wind velocity, and water temperature, influenced by atmospheric temperature and solar radiation are key factors for development. It follows from this work, the dynamics of both populations of cyanobacteria as euglenoids in crop irrigated rice is controlled by many biotic and abiotic making knowledge of their diversity and behavior, in practice very difficult having to therefore, the need to employ different methodologies and continuous monitoring and prolonged. It follows further that the use of in vitro culture, while contributing to the research morfomoleculares, promotes populations changes and further complicates the charactrization of the species. |
publishDate |
2010 |
dc.date.issued.fl_str_mv |
2010-08-30 |
dc.date.accessioned.fl_str_mv |
2017-04-05 |
dc.date.available.fl_str_mv |
2017-04-05 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
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doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
VASCONCELLOS, Noeli Júlia Schussler de. Heterocitic cyanobacteria and red euglenophyceae in flooded rice agroecosystem. 2010. 101 f. Tese (Doutorado em Agronomia) - Universidade Federal de Santa Maria, Santa Maria, 2010. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/3323 |
identifier_str_mv |
VASCONCELLOS, Noeli Júlia Schussler de. Heterocitic cyanobacteria and red euglenophyceae in flooded rice agroecosystem. 2010. 101 f. Tese (Doutorado em Agronomia) - Universidade Federal de Santa Maria, Santa Maria, 2010. |
url |
http://repositorio.ufsm.br/handle/1/3323 |
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