Análise de estimulantes em suplementos alimentares e produtos naturais a base de plantas comercializados para fins de emagrecimento no Brasil
Ano de defesa: | 2015 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Santa Maria
|
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Ciências Farmacêuticas
|
Departamento: |
Farmacologia
|
País: |
BR
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.ufsm.br/handle/1/6042 |
Resumo: | The strong advertising appeal, the search for a good physique, and the improvement in physical performance, has risen the popularity of food supplements and other therapies with slimming supplements based on plant extracts. Since the law exempts food supplements from registration, they are easy targets for importation, commercialization e consumption by the population, which worsens malpractice cases of adulterations. Thus, the objectives of the present work were: research the presence of stimulants in food supplements for both slimming and weight gain; study the addition of synthetic caffeine in natural products based on plants used for weight loss; develop chromatographic methods using HPLC with photodiode detection to detect caffeine, salicin, ephedrine, hordenine, tyramine, octopamine and synephrine (including the L and D enantiomers). The stimulants were separated by reverse phase chromatography using octadecylsilane columns (C18). For L and D synephrine, chromatography with chiral phase of β-cyclodextrin was used. Detection limits varied from 0.02 mg L-1 to 0.35 mg L-1, while quantification limits ranged from 0.07 mg L-1 to 1.16 mg L-1 for the separation of stimulants in food supplements. For analyses of caffeine in herbal products, the LOD and LOQ were 0.048 mg L-1 and 0.16 mg L-1, respectively. For the anlysis of the enantiomers of p-synephrine, the L enantiomer presented a detection limit of 0.11 mg L-1 and quantification limit of 0.36 mg L-1, while the D enantiomer exhibited a detection limit of 0.41 mg L-1 and a quantification limit of 1.36 mg L-1. Each method developed was then applied to the sample, which were, dietary supplement samples (n = 47), herbal products (n = 100), and fruits of Citrus aurantium. Caffeine was the most frequent of the studied stimulants, often exceeding the labeled content and the maximum dose per day recommended (420 mg). The food supplements presented caffeine, synephrine and ephedrine as main stimulants in 53% of the studied samples. |
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2016-10-312016-10-312015-02-27ZEMOLIN, Gabriela Mezzomo. Analysis of stimulants in dietary supplements and plant-based natural products marketed for weight loss in Brazil. 2015. 115 f. Dissertação (Mestrado em Farmacologia) - Universidade Federal de Santa Maria, Santa Maria, 2015.http://repositorio.ufsm.br/handle/1/6042The strong advertising appeal, the search for a good physique, and the improvement in physical performance, has risen the popularity of food supplements and other therapies with slimming supplements based on plant extracts. Since the law exempts food supplements from registration, they are easy targets for importation, commercialization e consumption by the population, which worsens malpractice cases of adulterations. Thus, the objectives of the present work were: research the presence of stimulants in food supplements for both slimming and weight gain; study the addition of synthetic caffeine in natural products based on plants used for weight loss; develop chromatographic methods using HPLC with photodiode detection to detect caffeine, salicin, ephedrine, hordenine, tyramine, octopamine and synephrine (including the L and D enantiomers). The stimulants were separated by reverse phase chromatography using octadecylsilane columns (C18). For L and D synephrine, chromatography with chiral phase of β-cyclodextrin was used. Detection limits varied from 0.02 mg L-1 to 0.35 mg L-1, while quantification limits ranged from 0.07 mg L-1 to 1.16 mg L-1 for the separation of stimulants in food supplements. For analyses of caffeine in herbal products, the LOD and LOQ were 0.048 mg L-1 and 0.16 mg L-1, respectively. For the anlysis of the enantiomers of p-synephrine, the L enantiomer presented a detection limit of 0.11 mg L-1 and quantification limit of 0.36 mg L-1, while the D enantiomer exhibited a detection limit of 0.41 mg L-1 and a quantification limit of 1.36 mg L-1. Each method developed was then applied to the sample, which were, dietary supplement samples (n = 47), herbal products (n = 100), and fruits of Citrus aurantium. Caffeine was the most frequent of the studied stimulants, often exceeding the labeled content and the maximum dose per day recommended (420 mg). The food supplements presented caffeine, synephrine and ephedrine as main stimulants in 53% of the studied samples.O forte apelo do mercado publicitário, aliado à busca por um corpo padrão e à melhora no desempenho físico faz com que a popularidade dos suplementos alimentares e a associação destes com outras terapias envolvendo compostos emagrecedores a base de plantas seja cada vez maior. A lei que isenta de registro os suplementos alimentares, os torna alvo de fácil importação, comercialização e consumo pela população em geral, agravando ainda a prática de adulteração sobre os mesmos. Logo, este trabalho teve como objetivos: pesquisar a presença de estimulantes em suplementos alimentares para fins de emagrecimento, estudar a incidência de cafeína sintética adicionada em produtos naturais à base de plantas utilizadas para perda de peso; desenvolver métodos cromatográficos emplregando HPLC com detecção com arranjo de fotodiodos (DAD) para a determinação de cafeína, salicina, efedrina, hordenina, tiramina, octopamina e sinefrina (incluindo seus enantiômeros L e D). Os estimulantes foram separados por cromatografia de fase reversa em colunas de octadecilsilano (C18) e os enantiômeros por cromatografia de par iônico com fase quiral de β ciclodextrina. Os limites de detecção (LD) variaram de 0,02 mg L-1 a 0,35 mg L-1, já os limites de quantificação (LQ) ficaram na faixa de 0,07 mg L-1 e 1,16 mg L-1 na separação dos estimulantes em suplementos alimentares. Para as análises de cafeína em produtos naturais a base de plantas, os LD e LQ foram 0,048 mg L-1 e 0,16 mg L-1 respectivamente. Para os enantiômeros da p-sinefrina, o enantiômero L apresentou LD de 0,11 mg L-1 e LQ de 0,36 mg L-1, já o enantiômero D exibiu um LD de 0,41 mg L-1 e LQ de 1,36 mg L-1. Cada método desenvolvido foi posteriormente aplicado em amostras de suplementos alimentares (n=47), produtos naturais à base de plantas (n=100) e fruto de Citrus aurantium. Dos compostos estudados, a cafeína foi a que se apresentou mais frequente, muitas vezes excedendo os teores rotulados nos produtos, ou então a dose máxima recomendada por dia (420 mg). Já os suplementos alimentares apresentaram cafeína, sinefrina e efedrina como principais estimulantes em 53% das amostras estudadas.application/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em Ciências FarmacêuticasUFSMBRFarmacologiaHPLCTermogênicosSuplementos alimentaresSinefrinaCafeínaCitrus aurantiumThermogenicFood supplementsSynephrineCaffeineCNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIAAnálise de estimulantes em suplementos alimentares e produtos naturais a base de plantas comercializados para fins de emagrecimento no BrasilAnalysis of stimulants in dietary supplements and plant-based natural products marketed for weight loss in Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisCarvalho, Leandro Machado dehttp://lattes.cnpq.br/6652387343920028Peres, Gustavo Tayarhttp://lattes.cnpq.br/3865245215376704Schaffazick, Scheila Rezendehttp://lattes.cnpq.br/3671495623581433http://lattes.cnpq.br/1317682036831236Zemolin, Gabriela Mezzomo2010000000004005003005005009da44f2c-6556-4846-9d66-386f290b802f6b2883da-7258-4e37-81eb-4eae9b89dadb0f0b54d1-f32c-4fac-b68b-6cb5f1f3dae40a663d9a-ea5e-4bbc-bc43-805607d10dacinfo:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALZEMOLIN, GABRIELA MEZZOMO.pdfapplication/pdf1326554http://repositorio.ufsm.br/bitstream/1/6042/1/ZEMOLIN%2c%20GABRIELA%20MEZZOMO.pdf3f9a9e11d17917c28996931198e578dcMD51TEXTZEMOLIN, GABRIELA MEZZOMO.pdf.txtZEMOLIN, GABRIELA MEZZOMO.pdf.txtExtracted texttext/plain186669http://repositorio.ufsm.br/bitstream/1/6042/2/ZEMOLIN%2c%20GABRIELA%20MEZZOMO.pdf.txtdf7a7743205aeecf5a1e03b79f18d260MD52THUMBNAILZEMOLIN, GABRIELA MEZZOMO.pdf.jpgZEMOLIN, GABRIELA MEZZOMO.pdf.jpgIM Thumbnailimage/jpeg5140http://repositorio.ufsm.br/bitstream/1/6042/3/ZEMOLIN%2c%20GABRIELA%20MEZZOMO.pdf.jpg09cf6279176b1c6fd513518701af97caMD531/60422022-05-02 15:54:16.295oai:repositorio.ufsm.br:1/6042Repositório Institucionalhttp://repositorio.ufsm.br/PUBhttp://repositorio.ufsm.br/oai/requestopendoar:39132022-05-02T18:54:16Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Análise de estimulantes em suplementos alimentares e produtos naturais a base de plantas comercializados para fins de emagrecimento no Brasil |
dc.title.alternative.eng.fl_str_mv |
Analysis of stimulants in dietary supplements and plant-based natural products marketed for weight loss in Brazil |
title |
Análise de estimulantes em suplementos alimentares e produtos naturais a base de plantas comercializados para fins de emagrecimento no Brasil |
spellingShingle |
Análise de estimulantes em suplementos alimentares e produtos naturais a base de plantas comercializados para fins de emagrecimento no Brasil Zemolin, Gabriela Mezzomo HPLC Termogênicos Suplementos alimentares Sinefrina Cafeína Citrus aurantium Thermogenic Food supplements Synephrine Caffeine CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA |
title_short |
Análise de estimulantes em suplementos alimentares e produtos naturais a base de plantas comercializados para fins de emagrecimento no Brasil |
title_full |
Análise de estimulantes em suplementos alimentares e produtos naturais a base de plantas comercializados para fins de emagrecimento no Brasil |
title_fullStr |
Análise de estimulantes em suplementos alimentares e produtos naturais a base de plantas comercializados para fins de emagrecimento no Brasil |
title_full_unstemmed |
Análise de estimulantes em suplementos alimentares e produtos naturais a base de plantas comercializados para fins de emagrecimento no Brasil |
title_sort |
Análise de estimulantes em suplementos alimentares e produtos naturais a base de plantas comercializados para fins de emagrecimento no Brasil |
author |
Zemolin, Gabriela Mezzomo |
author_facet |
Zemolin, Gabriela Mezzomo |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Carvalho, Leandro Machado de |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/6652387343920028 |
dc.contributor.referee1.fl_str_mv |
Peres, Gustavo Tayar |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/3865245215376704 |
dc.contributor.referee2.fl_str_mv |
Schaffazick, Scheila Rezende |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/3671495623581433 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/1317682036831236 |
dc.contributor.author.fl_str_mv |
Zemolin, Gabriela Mezzomo |
contributor_str_mv |
Carvalho, Leandro Machado de Peres, Gustavo Tayar Schaffazick, Scheila Rezende |
dc.subject.por.fl_str_mv |
HPLC Termogênicos Suplementos alimentares Sinefrina Cafeína |
topic |
HPLC Termogênicos Suplementos alimentares Sinefrina Cafeína Citrus aurantium Thermogenic Food supplements Synephrine Caffeine CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA |
dc.subject.eng.fl_str_mv |
Citrus aurantium Thermogenic Food supplements Synephrine Caffeine |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA |
description |
The strong advertising appeal, the search for a good physique, and the improvement in physical performance, has risen the popularity of food supplements and other therapies with slimming supplements based on plant extracts. Since the law exempts food supplements from registration, they are easy targets for importation, commercialization e consumption by the population, which worsens malpractice cases of adulterations. Thus, the objectives of the present work were: research the presence of stimulants in food supplements for both slimming and weight gain; study the addition of synthetic caffeine in natural products based on plants used for weight loss; develop chromatographic methods using HPLC with photodiode detection to detect caffeine, salicin, ephedrine, hordenine, tyramine, octopamine and synephrine (including the L and D enantiomers). The stimulants were separated by reverse phase chromatography using octadecylsilane columns (C18). For L and D synephrine, chromatography with chiral phase of β-cyclodextrin was used. Detection limits varied from 0.02 mg L-1 to 0.35 mg L-1, while quantification limits ranged from 0.07 mg L-1 to 1.16 mg L-1 for the separation of stimulants in food supplements. For analyses of caffeine in herbal products, the LOD and LOQ were 0.048 mg L-1 and 0.16 mg L-1, respectively. For the anlysis of the enantiomers of p-synephrine, the L enantiomer presented a detection limit of 0.11 mg L-1 and quantification limit of 0.36 mg L-1, while the D enantiomer exhibited a detection limit of 0.41 mg L-1 and a quantification limit of 1.36 mg L-1. Each method developed was then applied to the sample, which were, dietary supplement samples (n = 47), herbal products (n = 100), and fruits of Citrus aurantium. Caffeine was the most frequent of the studied stimulants, often exceeding the labeled content and the maximum dose per day recommended (420 mg). The food supplements presented caffeine, synephrine and ephedrine as main stimulants in 53% of the studied samples. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015-02-27 |
dc.date.accessioned.fl_str_mv |
2016-10-31 |
dc.date.available.fl_str_mv |
2016-10-31 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
ZEMOLIN, Gabriela Mezzomo. Analysis of stimulants in dietary supplements and plant-based natural products marketed for weight loss in Brazil. 2015. 115 f. Dissertação (Mestrado em Farmacologia) - Universidade Federal de Santa Maria, Santa Maria, 2015. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/6042 |
identifier_str_mv |
ZEMOLIN, Gabriela Mezzomo. Analysis of stimulants in dietary supplements and plant-based natural products marketed for weight loss in Brazil. 2015. 115 f. Dissertação (Mestrado em Farmacologia) - Universidade Federal de Santa Maria, Santa Maria, 2015. |
url |
http://repositorio.ufsm.br/handle/1/6042 |
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por |
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201000000000 |
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Universidade Federal de Santa Maria |
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Programa de Pós-Graduação em Ciências Farmacêuticas |
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UFSM |
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BR |
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Farmacologia |
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Universidade Federal de Santa Maria |
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