Estudo do perfil oxidativo e atividade da enzima acetilcolinesterase em pacientes com leucemia linfoblástica aguda (lla)
Ano de defesa: | 2008 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Santa Maria
|
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica
|
Departamento: |
Bioquímica
|
País: |
BR
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.ufsm.br/handle/1/11082 |
Resumo: | Acute lymphoblastic leukemia (ALL) is the most common cancer found in the pediatric population and is characterized by uncontrolled proliferation and maturation arrest of lymphoid progenitor cells (lymphoblasts) in the bone marrow. In ALL occurs an increase in the production of reactive species and studies show the involvement of oxidative stress in ALL pathogenesis. Moreover acetylcholinesterase (AChE) present in blood and plasma and also in lymphocytes is well established as having non-cholinergic functions including development of cancer and its contribution to the regulation of immune function. Considering such statements, it was investigated the oxidative profile and ACHE activity in ALL patients. Eighty two ALL patients and 50 healthy subjects participated in this study. Patients were divided into 4 groups: newly diagnosed (ND) remission induction (RI), remission maintenance (RM) and out-of-treatment (OT). For evaluation of the oxidative profile, we determined the catalase (CAT) and superoxide dismutase (SOD) activities in blood, thiols non protein (NPSH) in plasma and erythrocytes, as well as, vitamin E, TBARS and protein carbonyl content from serum samples. Damage markers in lipids and proteins, represented by MDA levels and protein carbonylation content respectively, showed increased levels in patients when compared with the control group. In relation to enzymatic defense, represented by the enzymes SOD and CAT, it was observed a decrease in the activities in ALL patients in groups RD, RI and RM, coming back to the normal in OT group. Non enzymatic defense (NPSH in plasma and vitamin E) analyzed also revealed decrease in ALL patients in relation to the controls. No significant difference in NPSH levels was observed between RD group and control group. In relation to AChE activity, in both total blood and lymphocytes, was observed an increase in the RD group in relation to the other groups. On the other hand, in RI and RM the enzyme activity was diminished in relation to control group. No significant difference was observed in AChE activity between FT group and the control group. The results suggest that in ALL an excessive accumulation of reactive species contributes to an antioxidant depletion and dysfunction. The results had also disclosed that the AChE activity is modified in ALL, what can suggest the involvement of this enzyme in the modulation of immune function and in cancer development. The ALL results in a systemic disorder that induces changes, which can be detected by mesuring the peripheral markers of oxidative stress and AChE activity in whole blood and lymphocytes. |
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2017-05-022017-05-022008-01-28BATTISTI, Vanessa. Study of oxidative profile and acetylcholinesterase activity in patients with acute lymphoblastic leukemia. 2008. 128 f. Dissertação (Mestrado em Bioquímica) - Universidade Federal de Santa Maria, Santa Maria, 2008.http://repositorio.ufsm.br/handle/1/11082Acute lymphoblastic leukemia (ALL) is the most common cancer found in the pediatric population and is characterized by uncontrolled proliferation and maturation arrest of lymphoid progenitor cells (lymphoblasts) in the bone marrow. In ALL occurs an increase in the production of reactive species and studies show the involvement of oxidative stress in ALL pathogenesis. Moreover acetylcholinesterase (AChE) present in blood and plasma and also in lymphocytes is well established as having non-cholinergic functions including development of cancer and its contribution to the regulation of immune function. Considering such statements, it was investigated the oxidative profile and ACHE activity in ALL patients. Eighty two ALL patients and 50 healthy subjects participated in this study. Patients were divided into 4 groups: newly diagnosed (ND) remission induction (RI), remission maintenance (RM) and out-of-treatment (OT). For evaluation of the oxidative profile, we determined the catalase (CAT) and superoxide dismutase (SOD) activities in blood, thiols non protein (NPSH) in plasma and erythrocytes, as well as, vitamin E, TBARS and protein carbonyl content from serum samples. Damage markers in lipids and proteins, represented by MDA levels and protein carbonylation content respectively, showed increased levels in patients when compared with the control group. In relation to enzymatic defense, represented by the enzymes SOD and CAT, it was observed a decrease in the activities in ALL patients in groups RD, RI and RM, coming back to the normal in OT group. Non enzymatic defense (NPSH in plasma and vitamin E) analyzed also revealed decrease in ALL patients in relation to the controls. No significant difference in NPSH levels was observed between RD group and control group. In relation to AChE activity, in both total blood and lymphocytes, was observed an increase in the RD group in relation to the other groups. On the other hand, in RI and RM the enzyme activity was diminished in relation to control group. No significant difference was observed in AChE activity between FT group and the control group. The results suggest that in ALL an excessive accumulation of reactive species contributes to an antioxidant depletion and dysfunction. The results had also disclosed that the AChE activity is modified in ALL, what can suggest the involvement of this enzyme in the modulation of immune function and in cancer development. The ALL results in a systemic disorder that induces changes, which can be detected by mesuring the peripheral markers of oxidative stress and AChE activity in whole blood and lymphocytes.A leucemia linfoblástica aguda (LLA) é o câncer mais comum na infância e é caracterizado por uma proliferação descontrolada de células linfóides imaturas (linfoblastos) na medula óssea. Na LLA há uma produção elevada de espécies reativas e estudos relatam que o estresse oxidativo está envolvido na patogenia desta doença. Além disso, a enzima acetilcolinesterase (AChE) presente no sangue e no plasma, inclusive nos linfócitos, desempenha funções não colinérgicas e parece estar envolvida na regulação da função imune e no desenvolvimento do câncer. Levando em consideração tais afirmativas, este trabalho investigou o perfil oxidativo e a atividade da enzima AChE em pacientes com LLA. Participaram do estudo 82 pacientes com LLA e 50 indivíduos controles. Os pacientes foram divididos em 4 grupos: recém diagnosticado (RD), indução da remissão (IR), manutenção da remissão (MR) e fora de tratamento (FT). Para avaliação do perfil oxidativo, determinou-se a atividade da catalase (CAT) e da superóxido dismutase (SOD) em sangue total, os níveis de tióis não protéicos (NPSH) em plasma e eritrócitos, bem como, a concentração de vitamina E, substâncias reativas ao ácido tiobarbitúrico (TBARS) e o conteúdo de proteína carbonil em amostras de soro da população estudada. Os marcadores de danos oxidativos em lipídeos e proteínas, representados pelos níveis de MDA e conteúdo de proteína carbonil mostraram-se elevados nos pacientes quando comparados ao grupo controle. Em relação aos antioxidantes enzimáticos, representados pelas enzimas SOD e CAT, observou-se uma diminuição nas atividades das mesmas nos pacientes com LLA nos grupos RD, IR E MR voltando ao normal no grupo FT. Os antioxidantes não enzimáticos analisados (NPSH em plasma e vitamina E) também se mostraram diminuídos nos pacientes em relação aos controles. Nenhuma diferença nos níveis de NPSH em eritrócitos foi encontrada entre o grupo RD e o grupo controle. Em relação à atividade da AChE, observou-se um aumento nos pacientes do grupo RD em relação aos demais grupos tanto em sangue total quanto em linfócitos. Já, nos grupos IR e MR a atividade da enzima encontrou-se diminuída em relação ao grupo controle. Não houve diferença significativa na atividade da AChE entre o grupo FT e o grupo controle. Os resultados sugerem que na LLA, ocorre acumulação excessiva de espécies reativas levando a depleção ou disfunção das defesas antioxidantes tanto enzimáticas quanto não enzimáticas. Os resultados também revelaram que a atividade da AChE está modificada na LLA, o que pode sugerir o envolvimento desta enzima na modulação da função imune e no desenvolvimento do câncer. Conclui-se então que na LLA ocorrem mudanças sistêmicas, as quais podem ser detectadas pela medida de marcadores periféricos do estresse oxidativo e pela atividade da AChE em linfócitos e sangue total.Conselho Nacional de Desenvolvimento Científico e Tecnológicoapplication/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em Ciências Biológicas: Bioquímica ToxicológicaUFSMBRBioquímicaLeucemia linfoblástica agudaEstresse oxidativoAcetilcolinesteraseLinfócitoAcute lymphoblastic leukemiaOxidative stressLymphocyteAcetylcholinesteraseCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICAEstudo do perfil oxidativo e atividade da enzima acetilcolinesterase em pacientes com leucemia linfoblástica aguda (lla)Study of oxidative profile and acetylcholinesterase activity in patients with acute lymphoblastic leukemiainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisMorsch, Vera Maria Melchiorshttp://lattes.cnpq.br/1519648219507868Schetinger, Maria Rosa Chitolinahttp://lattes.cnpq.br/4401319386725357Graça, Dominguita Luhershttp://lattes.cnpq.br/7761878749916936Loro, Vania Luciahttp://lattes.cnpq.br/6392817606416780http://lattes.cnpq.br/7800962078410048Battisti, Vanessa20080000000240050050050050050005cf99a1-4e49-4daa-bb3b-26eee86569c1fcada423-c98d-431b-a1f6-e4ad8cf3465ee31536a8-f5a8-4f54-b813-f9fc8b2e26865b1f94f9-23f7-49df-a620-c35a152c9039301a3550-6b95-45aa-be7f-19ffad13abc4info:eu-repo/semantics/openAccessreponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALVANESSA BATTISTI.pdfapplication/pdf1643439http://repositorio.ufsm.br/bitstream/1/11082/1/VANESSA%20BATTISTI.pdf699d882bcde6d99544de9eb4d49c35daMD51TEXTVANESSA BATTISTI.pdf.txtVANESSA BATTISTI.pdf.txtExtracted texttext/plain181723http://repositorio.ufsm.br/bitstream/1/11082/2/VANESSA%20BATTISTI.pdf.txtc2b99405a40f02044e1a1f0c4c564f7eMD52THUMBNAILVANESSA BATTISTI.pdf.jpgVANESSA BATTISTI.pdf.jpgIM Thumbnailimage/jpeg5620http://repositorio.ufsm.br/bitstream/1/11082/3/VANESSA%20BATTISTI.pdf.jpg7fa834a74371dc402f2f9f4021a36eeaMD531/110822023-04-17 10:43:08.065oai:repositorio.ufsm.br:1/11082Repositório Institucionalhttp://repositorio.ufsm.br/PUBhttp://repositorio.ufsm.br/oai/requestopendoar:39132023-04-17T13:43:08Manancial - Repositório Digital da UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Estudo do perfil oxidativo e atividade da enzima acetilcolinesterase em pacientes com leucemia linfoblástica aguda (lla) |
dc.title.alternative.eng.fl_str_mv |
Study of oxidative profile and acetylcholinesterase activity in patients with acute lymphoblastic leukemia |
title |
Estudo do perfil oxidativo e atividade da enzima acetilcolinesterase em pacientes com leucemia linfoblástica aguda (lla) |
spellingShingle |
Estudo do perfil oxidativo e atividade da enzima acetilcolinesterase em pacientes com leucemia linfoblástica aguda (lla) Battisti, Vanessa Leucemia linfoblástica aguda Estresse oxidativo Acetilcolinesterase Linfócito Acute lymphoblastic leukemia Oxidative stress Lymphocyte Acetylcholinesterase CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
title_short |
Estudo do perfil oxidativo e atividade da enzima acetilcolinesterase em pacientes com leucemia linfoblástica aguda (lla) |
title_full |
Estudo do perfil oxidativo e atividade da enzima acetilcolinesterase em pacientes com leucemia linfoblástica aguda (lla) |
title_fullStr |
Estudo do perfil oxidativo e atividade da enzima acetilcolinesterase em pacientes com leucemia linfoblástica aguda (lla) |
title_full_unstemmed |
Estudo do perfil oxidativo e atividade da enzima acetilcolinesterase em pacientes com leucemia linfoblástica aguda (lla) |
title_sort |
Estudo do perfil oxidativo e atividade da enzima acetilcolinesterase em pacientes com leucemia linfoblástica aguda (lla) |
author |
Battisti, Vanessa |
author_facet |
Battisti, Vanessa |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Morsch, Vera Maria Melchiors |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/1519648219507868 |
dc.contributor.advisor-co1.fl_str_mv |
Schetinger, Maria Rosa Chitolina |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/4401319386725357 |
dc.contributor.referee1.fl_str_mv |
Graça, Dominguita Luhers |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/7761878749916936 |
dc.contributor.referee2.fl_str_mv |
Loro, Vania Lucia |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/6392817606416780 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/7800962078410048 |
dc.contributor.author.fl_str_mv |
Battisti, Vanessa |
contributor_str_mv |
Morsch, Vera Maria Melchiors Schetinger, Maria Rosa Chitolina Graça, Dominguita Luhers Loro, Vania Lucia |
dc.subject.por.fl_str_mv |
Leucemia linfoblástica aguda Estresse oxidativo Acetilcolinesterase Linfócito |
topic |
Leucemia linfoblástica aguda Estresse oxidativo Acetilcolinesterase Linfócito Acute lymphoblastic leukemia Oxidative stress Lymphocyte Acetylcholinesterase CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
dc.subject.eng.fl_str_mv |
Acute lymphoblastic leukemia Oxidative stress Lymphocyte Acetylcholinesterase |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
description |
Acute lymphoblastic leukemia (ALL) is the most common cancer found in the pediatric population and is characterized by uncontrolled proliferation and maturation arrest of lymphoid progenitor cells (lymphoblasts) in the bone marrow. In ALL occurs an increase in the production of reactive species and studies show the involvement of oxidative stress in ALL pathogenesis. Moreover acetylcholinesterase (AChE) present in blood and plasma and also in lymphocytes is well established as having non-cholinergic functions including development of cancer and its contribution to the regulation of immune function. Considering such statements, it was investigated the oxidative profile and ACHE activity in ALL patients. Eighty two ALL patients and 50 healthy subjects participated in this study. Patients were divided into 4 groups: newly diagnosed (ND) remission induction (RI), remission maintenance (RM) and out-of-treatment (OT). For evaluation of the oxidative profile, we determined the catalase (CAT) and superoxide dismutase (SOD) activities in blood, thiols non protein (NPSH) in plasma and erythrocytes, as well as, vitamin E, TBARS and protein carbonyl content from serum samples. Damage markers in lipids and proteins, represented by MDA levels and protein carbonylation content respectively, showed increased levels in patients when compared with the control group. In relation to enzymatic defense, represented by the enzymes SOD and CAT, it was observed a decrease in the activities in ALL patients in groups RD, RI and RM, coming back to the normal in OT group. Non enzymatic defense (NPSH in plasma and vitamin E) analyzed also revealed decrease in ALL patients in relation to the controls. No significant difference in NPSH levels was observed between RD group and control group. In relation to AChE activity, in both total blood and lymphocytes, was observed an increase in the RD group in relation to the other groups. On the other hand, in RI and RM the enzyme activity was diminished in relation to control group. No significant difference was observed in AChE activity between FT group and the control group. The results suggest that in ALL an excessive accumulation of reactive species contributes to an antioxidant depletion and dysfunction. The results had also disclosed that the AChE activity is modified in ALL, what can suggest the involvement of this enzyme in the modulation of immune function and in cancer development. The ALL results in a systemic disorder that induces changes, which can be detected by mesuring the peripheral markers of oxidative stress and AChE activity in whole blood and lymphocytes. |
publishDate |
2008 |
dc.date.issued.fl_str_mv |
2008-01-28 |
dc.date.accessioned.fl_str_mv |
2017-05-02 |
dc.date.available.fl_str_mv |
2017-05-02 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
BATTISTI, Vanessa. Study of oxidative profile and acetylcholinesterase activity in patients with acute lymphoblastic leukemia. 2008. 128 f. Dissertação (Mestrado em Bioquímica) - Universidade Federal de Santa Maria, Santa Maria, 2008. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/11082 |
identifier_str_mv |
BATTISTI, Vanessa. Study of oxidative profile and acetylcholinesterase activity in patients with acute lymphoblastic leukemia. 2008. 128 f. Dissertação (Mestrado em Bioquímica) - Universidade Federal de Santa Maria, Santa Maria, 2008. |
url |
http://repositorio.ufsm.br/handle/1/11082 |
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por |
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200800000002 |
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