Isolamento e diferenciação das células-tronco da polpa dentária canina em células progenitoras neurais
Ano de defesa: | 2018 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Santa Maria
Centro de Ciências Rurais |
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Medicina Veterinária
|
Departamento: |
Medicina Veterinária
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.ufsm.br/handle/1/14208 |
Resumo: | The development of research that promotes the understanding of the mechanisms of neurogenesis is important for the treatment of many degenerative neurological diseases in humans and animals. However, obtaining neural stem cells for isolation and in vitro culture requires the collection of tissues vital to the patient. The restriction of access of these tissues and limitations in experimental practice has encouraged researchers to seek alternatives using the differentiation potential presented in stem cells of the adult individual. The aim of the study was to verify the differentiation capacity of canine dental pulp stem cells in neural progenitor cells when stimulated in vitro, and quantify the attainment and viability of the undifferentiated cells, during three cell culture passages. Dental pulp cells were isolated from two dogs, with ten months age around, that died due to automobile trauma. Cells were expanded for three days in cell culture and cell number and cell viability evaluations were performed. Subsequently, neural differentiation was performed in induction medium culture. The post-induction immunophenotypic profile was evaluated using flow cytometry and fluorescent immunocytochemistry tests. Spherical cell groupings, consistent with neurospheres, were observed seven days after. It was possible to verify high expression of anti-nestin and anti-GFAP neural markers after 14 days, suggesting that the undifferentiated dental pulp canine stem cells are able to present neural differentiation, through appropriate stimuli. Dental pulp canine has been shown to be highly capable of delivering viable undifferentiated stem cells. |
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2018-09-05T16:36:08Z2018-09-05T16:36:08Z2018-02-26http://repositorio.ufsm.br/handle/1/14208The development of research that promotes the understanding of the mechanisms of neurogenesis is important for the treatment of many degenerative neurological diseases in humans and animals. However, obtaining neural stem cells for isolation and in vitro culture requires the collection of tissues vital to the patient. The restriction of access of these tissues and limitations in experimental practice has encouraged researchers to seek alternatives using the differentiation potential presented in stem cells of the adult individual. The aim of the study was to verify the differentiation capacity of canine dental pulp stem cells in neural progenitor cells when stimulated in vitro, and quantify the attainment and viability of the undifferentiated cells, during three cell culture passages. Dental pulp cells were isolated from two dogs, with ten months age around, that died due to automobile trauma. Cells were expanded for three days in cell culture and cell number and cell viability evaluations were performed. Subsequently, neural differentiation was performed in induction medium culture. The post-induction immunophenotypic profile was evaluated using flow cytometry and fluorescent immunocytochemistry tests. Spherical cell groupings, consistent with neurospheres, were observed seven days after. It was possible to verify high expression of anti-nestin and anti-GFAP neural markers after 14 days, suggesting that the undifferentiated dental pulp canine stem cells are able to present neural differentiation, through appropriate stimuli. Dental pulp canine has been shown to be highly capable of delivering viable undifferentiated stem cells.O desenvolvimento de pesquisas que promovam o entendimento dos mecanismos da neurogênese é de fundamental importância para o tratamento de muitas doenças neurológicas degenerativas em humanos e animais. Contudo, a obtenção de célulastronco neurais para isolamento e cultivo in vitro, necessita coleta de tecidos vitais para o paciente. A restrição ao acesso destes tecidos, bem como limitações na prática experimental, tem incentivado pesquisadores a buscar alternativas utilizando o potencial de diferenciação apresentado por células-tronco encontradas no indivíduo adulto. Neste contexto, o objetivo do presente estudo foi verificar a capacidade de diferenciação das células-tronco da polpa dentária canina em células progenitoras neurais, quando estimuladas in vitro, assim como quantificar a obtenção e viabilidade das células indiferenciadas, ao longo de três passagens em cultura celular. Foram isoladas células pulpares de dois cães, com aproximadamente dez meses de idade, que apresentaram óbito em decorrência de traumatismo automotivo. As células foram expandidas durante três passagem em cultura celular, onde foram realizadas avaliações de viabilidade e obtenção celular. Posteriormente, foram expostas a cultivo em meio indutor a diferenciação neural. O perfil imunofenotípico pós-indução, foi avaliado utilizando testes de citometria de fluxo e imunocitoquímica fluorescente. Após sete dias de cultura indutora neural, foram observadas formações de agrupamentos celulares esféricos, condizentes com neuroesferas. Após 14 dias, foi possível verificar elevada expressão de marcadores neurais anti-nestina e anti-GFAP, sugerindo que as células-tronco indiferenciadas da polpa dentária canina são capazes de apresentar diferenciação neural, mediante estímulos adequados. A polpa dentária canina demonstrou elevada capacidade em fornecer células-tronco indiferenciadas altamente viáveis.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESporUniversidade Federal de Santa MariaCentro de Ciências RuraisPrograma de Pós-Graduação em Medicina VeterináriaUFSMBrasilMedicina VeterináriaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessNeuroesferaPolpa dentáriaTerapia celularNeurospherDental pulpCell therapyCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIAIsolamento e diferenciação das células-tronco da polpa dentária canina em células progenitoras neuraisIsolation and differentiation of canine dental pulp stem cells in neural progenitor cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisMüller, Daniel Curvello de Mendonçahttp://lattes.cnpq.br/9457849435452537Krause, Luciana Maria Fontanarihttp://lattes.cnpq.br/9844890896121847Fighera, Rafael Almeidahttp://lattes.cnpq.br/6223365736139655http://lattes.cnpq.br/2468513083889485Rosa, Matheus Pippi da50050000000760084e9758f-b260-4ee0-9782-876b731a7f9a6ba3cbc6-d52e-467e-8687-69cdddb50a47a310dec0-114a-4a8c-836d-d08756d182991023603b-d159-4bb2-926d-915f37395fd9reponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALDIS_PPGMV_2018_ROSA_MATHEUS.pdfDIS_PPGMV_2018_ROSA_MATHEUS.pdfDissertação de Mestradoapplication/pdf938526http://repositorio.ufsm.br/bitstream/1/14208/1/DIS_PPGMV_2018_ROSA_MATHEUS.pdf34bf3cffb388128feddf7245c2099f4bMD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; 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dc.title.por.fl_str_mv |
Isolamento e diferenciação das células-tronco da polpa dentária canina em células progenitoras neurais |
dc.title.alternative.eng.fl_str_mv |
Isolation and differentiation of canine dental pulp stem cells in neural progenitor cells |
title |
Isolamento e diferenciação das células-tronco da polpa dentária canina em células progenitoras neurais |
spellingShingle |
Isolamento e diferenciação das células-tronco da polpa dentária canina em células progenitoras neurais Rosa, Matheus Pippi da Neuroesfera Polpa dentária Terapia celular Neurospher Dental pulp Cell therapy CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Isolamento e diferenciação das células-tronco da polpa dentária canina em células progenitoras neurais |
title_full |
Isolamento e diferenciação das células-tronco da polpa dentária canina em células progenitoras neurais |
title_fullStr |
Isolamento e diferenciação das células-tronco da polpa dentária canina em células progenitoras neurais |
title_full_unstemmed |
Isolamento e diferenciação das células-tronco da polpa dentária canina em células progenitoras neurais |
title_sort |
Isolamento e diferenciação das células-tronco da polpa dentária canina em células progenitoras neurais |
author |
Rosa, Matheus Pippi da |
author_facet |
Rosa, Matheus Pippi da |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Müller, Daniel Curvello de Mendonça |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/9457849435452537 |
dc.contributor.referee1.fl_str_mv |
Krause, Luciana Maria Fontanari |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/9844890896121847 |
dc.contributor.referee2.fl_str_mv |
Fighera, Rafael Almeida |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/6223365736139655 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/2468513083889485 |
dc.contributor.author.fl_str_mv |
Rosa, Matheus Pippi da |
contributor_str_mv |
Müller, Daniel Curvello de Mendonça Krause, Luciana Maria Fontanari Fighera, Rafael Almeida |
dc.subject.por.fl_str_mv |
Neuroesfera Polpa dentária Terapia celular |
topic |
Neuroesfera Polpa dentária Terapia celular Neurospher Dental pulp Cell therapy CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
dc.subject.eng.fl_str_mv |
Neurospher Dental pulp Cell therapy |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
The development of research that promotes the understanding of the mechanisms of neurogenesis is important for the treatment of many degenerative neurological diseases in humans and animals. However, obtaining neural stem cells for isolation and in vitro culture requires the collection of tissues vital to the patient. The restriction of access of these tissues and limitations in experimental practice has encouraged researchers to seek alternatives using the differentiation potential presented in stem cells of the adult individual. The aim of the study was to verify the differentiation capacity of canine dental pulp stem cells in neural progenitor cells when stimulated in vitro, and quantify the attainment and viability of the undifferentiated cells, during three cell culture passages. Dental pulp cells were isolated from two dogs, with ten months age around, that died due to automobile trauma. Cells were expanded for three days in cell culture and cell number and cell viability evaluations were performed. Subsequently, neural differentiation was performed in induction medium culture. The post-induction immunophenotypic profile was evaluated using flow cytometry and fluorescent immunocytochemistry tests. Spherical cell groupings, consistent with neurospheres, were observed seven days after. It was possible to verify high expression of anti-nestin and anti-GFAP neural markers after 14 days, suggesting that the undifferentiated dental pulp canine stem cells are able to present neural differentiation, through appropriate stimuli. Dental pulp canine has been shown to be highly capable of delivering viable undifferentiated stem cells. |
publishDate |
2018 |
dc.date.accessioned.fl_str_mv |
2018-09-05T16:36:08Z |
dc.date.available.fl_str_mv |
2018-09-05T16:36:08Z |
dc.date.issued.fl_str_mv |
2018-02-26 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
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http://repositorio.ufsm.br/handle/1/14208 |
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http://repositorio.ufsm.br/handle/1/14208 |
dc.language.iso.fl_str_mv |
por |
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por |
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500500000007 |
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600 |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
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openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Rurais |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Medicina Veterinária |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Medicina Veterinária |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Rurais |
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