O elemento transponível mariner e o acúmulo de transposição somática no desenvolvimento de Drosophila
Ano de defesa: | 2017 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Santa Maria
Centro de Ciências Naturais e Exatas |
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Biodiversidade Animal
|
Departamento: |
Bioquímica
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.ufsm.br/handle/1/17882 |
Resumo: | Transposable elements (TEs) are sequences of DNA with the ability to move inside host genomes. Some TEs are able to transpose into somatic tissues, process called Somatic Transposition (ST) that can be involved with aging, neurodegenerative diseases and cancer. The mariner element is a TE able to move in both somatic cells and germline. A nonautonomous mariner was discovered as a cause of the white-peach mutation in Drosophila eyes. The insertion of this element in the promoter region of the white gene leads to flies with peach colored eyes. In the presence of autonomous copies, the mariner in the white gene can transpose, reversing the mutation and leading to flies with mosaic phenotype (white-peach colored eyes with red spots). This model makes possible to measure the rate of ST by the number of red spots in mosaic flies, because every spot represents an excision event and as a consequence, a ST event. In this study, we used mild heat stress to induce mariner transposition through different stages of D. simulans white-peach test development in order to verify if ST events accumulate in Drosophila. The mariner ST was not constant during the development: the larval stage showed the higher ST rates; pupal stage exhibited less than larval; and transposition was not detected during the embryogenesis. The development stage and the cellular process involved in this period can affect mariner transposition: more intense cell division phases may facilitate it. ST accumulation does not occur linearly, but it may vary by the stages with highest or lowest transposition. The mariner activation under thermal stress was confirmed by qPCR that indicated twice as high rates of ST in the larval stage at 28ºC. Besides, these analyses also showed an accumulation of ST throughout the development. |
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2019-08-09T19:21:15Z2019-08-09T19:21:15Z2017-03-20http://repositorio.ufsm.br/handle/1/17882Transposable elements (TEs) are sequences of DNA with the ability to move inside host genomes. Some TEs are able to transpose into somatic tissues, process called Somatic Transposition (ST) that can be involved with aging, neurodegenerative diseases and cancer. The mariner element is a TE able to move in both somatic cells and germline. A nonautonomous mariner was discovered as a cause of the white-peach mutation in Drosophila eyes. The insertion of this element in the promoter region of the white gene leads to flies with peach colored eyes. In the presence of autonomous copies, the mariner in the white gene can transpose, reversing the mutation and leading to flies with mosaic phenotype (white-peach colored eyes with red spots). This model makes possible to measure the rate of ST by the number of red spots in mosaic flies, because every spot represents an excision event and as a consequence, a ST event. In this study, we used mild heat stress to induce mariner transposition through different stages of D. simulans white-peach test development in order to verify if ST events accumulate in Drosophila. The mariner ST was not constant during the development: the larval stage showed the higher ST rates; pupal stage exhibited less than larval; and transposition was not detected during the embryogenesis. The development stage and the cellular process involved in this period can affect mariner transposition: more intense cell division phases may facilitate it. ST accumulation does not occur linearly, but it may vary by the stages with highest or lowest transposition. The mariner activation under thermal stress was confirmed by qPCR that indicated twice as high rates of ST in the larval stage at 28ºC. Besides, these analyses also showed an accumulation of ST throughout the development.Elementos transponíveis (TEs) são sequências de DNA móveis nos genomas hospedeiros. Alguns TEs são capazes de se transpor em células somáticas, processo conhecido por Transposição Somática (TS), que pode estar envolvido no envelhecimento celular, doenças neurodegenerativas e câncer. O elemento mariner é um TE capaz de se transpor em células somáticas e germinativas. Uma cópia não-autônoma desse elemento na região promotora do gene white gera moscas com olhos cor de pêssego, chamadas white-peach. Na presença de cópias autônomas, o mariner no gene white é capaz de se transpor, revertendo parcialmente a mutação e originando moscas com fenótipo mosaico: olhos white-peach com manchas vermelhas. Esse modelo torna possível medir a taxa de TS através do número de manchas das moscas mosaico, pois cada mancha reflete um evento de excisão e como consequência, um evento de TS. Neste estudo utilizamos o estresse por temperatura para induzir a transposição de mariner durante diferentes estágios do desenvolvimento de D. simulans white-peach teste, buscando verificar se eventos de TS são acumulados em Drosophila. A TS de mariner sob estresse térmico não foi constante durante todo o desenvolvimento: a fase larval apresentou as maiores taxas de TS; a fase de pupa exibiu taxas menores; e na fase embrionária a transposição não foi detectada. A fase do desenvolvimento e os processos celulares envolvidos nesse período podem influenciar a transposição de mariner, que parece ser facilitada por estágios de intensa divisão celular, como a fase larval. O acúmulo de TS não ocorre de maneira linear, podendo variar de acordo com os estágios de maior e menor transposição. A ativação de mariner sob estresse foi confirmada por qPCR, que indicou uma taxa de TS duas vezes maior nos tratamentos a 28°C para a fase larval. Essa análise também mostrou um acúmulo de TS ao longo do desenvolvimento.Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPqporUniversidade Federal de Santa MariaCentro de Ciências Naturais e ExatasPrograma de Pós-Graduação em Biodiversidade AnimalUFSMBrasilBioquímicaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessElementos transponíveisTransposição somáticaMarinerDrosophilaTransposable elementsSomatic transpositionCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICAO elemento transponível mariner e o acúmulo de transposição somática no desenvolvimento de DrosophilaThe mariner transposable element and accumulation of somatic transposition in Drosophila developmentinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisLoreto, Elgion Lucio da Silvahttp://lattes.cnpq.br/6493669115018157Herédia, Fabiana de Oliveirahttp://lattes.cnpq.br/3998323160840420Araújo, Daniel Mendes Pereira Ardisson dehttp://lattes.cnpq.br/5900778605189135http://lattes.cnpq.br/8396715425041735Pereira, Camila de Moura2008000000026001ba859a2-58b7-4e36-9d19-b101c4d2f6693508a001-7496-4cf1-a9d7-bcb6c120ba5b659c2519-67a0-4902-b842-3b2f6d0860a5970a0c6b-6739-4b09-a563-335ac39788cereponame:Manancial - Repositório Digital da UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMCC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; 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dc.title.por.fl_str_mv |
O elemento transponível mariner e o acúmulo de transposição somática no desenvolvimento de Drosophila |
dc.title.alternative.eng.fl_str_mv |
The mariner transposable element and accumulation of somatic transposition in Drosophila development |
title |
O elemento transponível mariner e o acúmulo de transposição somática no desenvolvimento de Drosophila |
spellingShingle |
O elemento transponível mariner e o acúmulo de transposição somática no desenvolvimento de Drosophila Pereira, Camila de Moura Elementos transponíveis Transposição somática Mariner Drosophila Transposable elements Somatic transposition CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
title_short |
O elemento transponível mariner e o acúmulo de transposição somática no desenvolvimento de Drosophila |
title_full |
O elemento transponível mariner e o acúmulo de transposição somática no desenvolvimento de Drosophila |
title_fullStr |
O elemento transponível mariner e o acúmulo de transposição somática no desenvolvimento de Drosophila |
title_full_unstemmed |
O elemento transponível mariner e o acúmulo de transposição somática no desenvolvimento de Drosophila |
title_sort |
O elemento transponível mariner e o acúmulo de transposição somática no desenvolvimento de Drosophila |
author |
Pereira, Camila de Moura |
author_facet |
Pereira, Camila de Moura |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Loreto, Elgion Lucio da Silva |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/6493669115018157 |
dc.contributor.referee1.fl_str_mv |
Herédia, Fabiana de Oliveira |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/3998323160840420 |
dc.contributor.referee2.fl_str_mv |
Araújo, Daniel Mendes Pereira Ardisson de |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/5900778605189135 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/8396715425041735 |
dc.contributor.author.fl_str_mv |
Pereira, Camila de Moura |
contributor_str_mv |
Loreto, Elgion Lucio da Silva Herédia, Fabiana de Oliveira Araújo, Daniel Mendes Pereira Ardisson de |
dc.subject.por.fl_str_mv |
Elementos transponíveis Transposição somática Mariner Drosophila |
topic |
Elementos transponíveis Transposição somática Mariner Drosophila Transposable elements Somatic transposition CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
dc.subject.eng.fl_str_mv |
Transposable elements Somatic transposition |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
description |
Transposable elements (TEs) are sequences of DNA with the ability to move inside host genomes. Some TEs are able to transpose into somatic tissues, process called Somatic Transposition (ST) that can be involved with aging, neurodegenerative diseases and cancer. The mariner element is a TE able to move in both somatic cells and germline. A nonautonomous mariner was discovered as a cause of the white-peach mutation in Drosophila eyes. The insertion of this element in the promoter region of the white gene leads to flies with peach colored eyes. In the presence of autonomous copies, the mariner in the white gene can transpose, reversing the mutation and leading to flies with mosaic phenotype (white-peach colored eyes with red spots). This model makes possible to measure the rate of ST by the number of red spots in mosaic flies, because every spot represents an excision event and as a consequence, a ST event. In this study, we used mild heat stress to induce mariner transposition through different stages of D. simulans white-peach test development in order to verify if ST events accumulate in Drosophila. The mariner ST was not constant during the development: the larval stage showed the higher ST rates; pupal stage exhibited less than larval; and transposition was not detected during the embryogenesis. The development stage and the cellular process involved in this period can affect mariner transposition: more intense cell division phases may facilitate it. ST accumulation does not occur linearly, but it may vary by the stages with highest or lowest transposition. The mariner activation under thermal stress was confirmed by qPCR that indicated twice as high rates of ST in the larval stage at 28ºC. Besides, these analyses also showed an accumulation of ST throughout the development. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017-03-20 |
dc.date.accessioned.fl_str_mv |
2019-08-09T19:21:15Z |
dc.date.available.fl_str_mv |
2019-08-09T19:21:15Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
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http://repositorio.ufsm.br/handle/1/17882 |
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http://repositorio.ufsm.br/handle/1/17882 |
dc.language.iso.fl_str_mv |
por |
language |
por |
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200800000002 |
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600 |
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dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Naturais e Exatas |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Biodiversidade Animal |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Bioquímica |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Naturais e Exatas |
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