Consumo de mel fornece proteção contra alterações motoras e moleculares induzidas por hipóxia/reperfusão em Drosophila melanogaster
Ano de defesa: | 2017 |
---|---|
Autor(a) principal: | |
Orientador(a): | , |
Banca de defesa: | , , |
Tipo de documento: | Tese |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Santa Maria
Centro de Ciências Naturais e Exatas |
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica
|
Departamento: |
Ciências Biológicas
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.ufsm.br/handle/1/12449 |
Resumo: | Honey is a complex product produced by honeybees from nectar, being widely used for its sweetening properties, as well as for its benefits to human health. Honey presents anti-inflammatory, hypoglycemic and antioxidant properties. Hypoxia followed by reperfusion is a condition triggered in several pathologies and can culminate with oxidative tissue damage. The fruit-fly, Drosophila melanogaster, has hypoxia tolerant characteristics and homology with vertebrate from pathways involved in the cascade of cellular responses for hypoxia/reperfusion. Considering these aspects, the main objective of this work was to evaluate the possible protective effect of honey produced in the Brazilian Pampa Biome on changes induced by hypoxia/reperfusion in Drosophila melanogaster, with emphasis on oxidative stress. Wild flies were treated with a diet containing 10% honey for 3 days and subsequently submitted to hypoxia conditions (3hs). After reperfusion of oxygen, parameters of survival, behavior, oxidative stress as well as mRNA expression of genes related with metabolic, inflammation and death pathways were evaluated. The results demonstrated that the honey tested is composed of compounds with antioxidant activity that were recognized both in vitro and in vivo. Hypoxia/reperfusion induced an increase in the regulation of mRNA expression of Sima (HIF-1), NFκB, Nrf2, HOX, AKT-1, INR, dILP2, dILP5 and HSP27. Indeed, flies submitted to hypoxia/reperfusion has a expression decreased of EWG and Opa-1. Honey was able to reestablish the expression of Opa-1, a gene related to mitochondrial fusion. Honey treatment also reduced the changes induced by hypoxia/reperfusion in parameters of survival, locomotor deficits, wing position, reactive oxygen species (ROS) production, GST, caspases and mitochondrial complex I activities. The results obtained here show, for the first time, the beneficial effects of honey against changes induced by hypoxia/reperfusion in Drosophila melanogaster. This study evidenced that that the honey from the Brazilian Pampa Biome has a high quality and a significant antioxidant activity in vivo, which can be related with its beneficial effects against hypoxia/reperfusion. In addition, we consider the honey as well as Drosophila melanogaster promising targets for studying pathologies associated with oxidative stress and hypoxia/reperfusion. |
id |
UFSM_049ab777c7ee145101424d2dfe7df2ab |
---|---|
oai_identifier_str |
oai:repositorio.ufsm.br:1/12449 |
network_acronym_str |
UFSM |
network_name_str |
Biblioteca Digital de Teses e Dissertações do UFSM |
repository_id_str |
|
spelling |
2018-02-16T11:39:11Z2018-02-16T11:39:11Z2017-02-14http://repositorio.ufsm.br/handle/1/12449Honey is a complex product produced by honeybees from nectar, being widely used for its sweetening properties, as well as for its benefits to human health. Honey presents anti-inflammatory, hypoglycemic and antioxidant properties. Hypoxia followed by reperfusion is a condition triggered in several pathologies and can culminate with oxidative tissue damage. The fruit-fly, Drosophila melanogaster, has hypoxia tolerant characteristics and homology with vertebrate from pathways involved in the cascade of cellular responses for hypoxia/reperfusion. Considering these aspects, the main objective of this work was to evaluate the possible protective effect of honey produced in the Brazilian Pampa Biome on changes induced by hypoxia/reperfusion in Drosophila melanogaster, with emphasis on oxidative stress. Wild flies were treated with a diet containing 10% honey for 3 days and subsequently submitted to hypoxia conditions (3hs). After reperfusion of oxygen, parameters of survival, behavior, oxidative stress as well as mRNA expression of genes related with metabolic, inflammation and death pathways were evaluated. The results demonstrated that the honey tested is composed of compounds with antioxidant activity that were recognized both in vitro and in vivo. Hypoxia/reperfusion induced an increase in the regulation of mRNA expression of Sima (HIF-1), NFκB, Nrf2, HOX, AKT-1, INR, dILP2, dILP5 and HSP27. Indeed, flies submitted to hypoxia/reperfusion has a expression decreased of EWG and Opa-1. Honey was able to reestablish the expression of Opa-1, a gene related to mitochondrial fusion. Honey treatment also reduced the changes induced by hypoxia/reperfusion in parameters of survival, locomotor deficits, wing position, reactive oxygen species (ROS) production, GST, caspases and mitochondrial complex I activities. The results obtained here show, for the first time, the beneficial effects of honey against changes induced by hypoxia/reperfusion in Drosophila melanogaster. This study evidenced that that the honey from the Brazilian Pampa Biome has a high quality and a significant antioxidant activity in vivo, which can be related with its beneficial effects against hypoxia/reperfusion. In addition, we consider the honey as well as Drosophila melanogaster promising targets for studying pathologies associated with oxidative stress and hypoxia/reperfusion.O mel é um produto complexo produzido por abelhas melíferas a partir do néctar e bastante utilizado pelas suas propriedades edulcorantes, bem como pelos seus benefícios para a saúde humana. O mel apresenta atividades anti-inflamatória, hipoglicêmica e antioxidante. A hipóxia seguida de reperfusão é uma condição desencadeada em diversas patologias e pode culminar com danos teciduais de cunho oxidativo. A mosca-da-fruta, Drosophila melanogaster, possui características tolerantes a hipóxia e homologia com animais vertebrados de vias envolvidas na cascata de respostas celulares à hipoxia/reperfusão. Considerando tais aspectos, o objetivo principal desse trabalho foi avaliar os efeitos do mel produzido no Bioma Pampa Brasileiro sobre as alterações teciduais/moleculares induzidas por hipóxia/reperfusão em Drosophila melanogaster, com ênfase em estresse oxidativo. Moscas selvagens foram tratadas com 10% de mel por 3 dias e posteriormente submetidas à hipóxia (3hs). Após reperfusão de oxigênio, foram avaliados parâmetros de sobrevivência e comportamento; parâmetros de estresse oxidativo, bem como, a expressão de mRNA de genes envolvidos em vias metabólicas, inflamatórias e morte celular. Os resultados demonstraram que o mel testado é constituído de compostos com atividade antioxidante, que foi reconhecida tanto in vitro como in vivo. A hipóxia/reperfusão induziu um aumento na expressão do mRNA de Sima (HIF-1), LDH, NFκB, Nrf2, HOX, AKT-1, INR, dILP2, dILP5 e HSP27. As moscas submetidas a hipoxia/reperfusão também apresentaram expressão diminuída do mRNA do EWG e Opa-1. O mel foi efetivo em restabelecer a expressão alterada de Opa-1, gene relacionado com fusão mitocondrial. Além disso, o tratamento com mel reduziu as alterações induzidas por hipóxia/reperfusão sobre os parâmetros de sobrevivência, déficits locomotores, alteração da posição das asas, produção de espécies reativas de oxigênio (EROs), atividades da GST, caspases e complexo I da cadeia respiratória mitocondrial. Os resultados obtidos aqui mostram, pela primeira vez, os efeitos benéficos de mel contra alterações induzidas por hipóxia/reperfusão em Drosophila melanogaster. Este estudo evidenciou que o mel do Bioma Pampa Brasileiro tem além de um alto nível de qualidade, uma atividade antioxidante significativa in vivo, a qual pode estar relacionada com seus efeitos benéficos contra hipoxia/reperfusão. Com base nos resultados, considera-se ainda tanto o mel como a Drosophila melanogaster alvos promissores para estudos voltados para doenças associadas com condições de estresse e hipoxia/reperfusão.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESporUniversidade Federal de Santa MariaCentro de Ciências Naturais e ExatasPrograma de Pós-Graduação em Ciências Biológicas: Bioquímica ToxicológicaUFSMBrasilCiências BiológicasAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessMelAntioxidanteHipoxia/reperfusãoDrosophila melanogasterEstresse oxidativoHoneyAntioxidantHypoxia/reperfusionOxidative stressCNPQ::CIENCIAS BIOLOGICASConsumo de mel fornece proteção contra alterações motoras e moleculares induzidas por hipóxia/reperfusão em Drosophila melanogasterHoney consumption provides protection against motor and molecular changes induced by hypoxia / reperfusion in Drosophila melanogasterinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisFranco, Jeferson Luis52e40014-4a74-4171-aa9f-beaa648fd9bb600Fachinetto, Roseleihttp://lattes.cnpq.br/1680065573338339http://lattes.cnpq.br/7203076675431306Fachinetto, Roseleihttp://lattes.cnpq.br/7203076675431306Dalla Corte, Cristiane Lenzhttp://lattes.cnpq.br/5296284169605317Puntel, Robson Luizhttp://lattes.cnpq.br/1134532326779900http://lattes.cnpq.br/2555769571752143Cruz, Litiele Cezar da20000000000660016ea12f1-12c6-4435-a98c-cdeb5f7d1c597c74148e-291c-43ef-b5b6-8d69e2b8ca29d4a64262-0e7e-45c5-910e-0b0aee907c10ed6a73a6-ab4c-4b6e-ac2a-ec6b5c7c040be5162be2-b3cb-46b9-9e5a-bea0d2c8bc48reponame:Biblioteca Digital de Teses e Dissertações do UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALTES_PPGBT_2017_CRUZ_LITIELE.pdfTES_PPGBT_2017_CRUZ_LITIELE.pdfTese de Doutoradoapplication/pdf8698797http://repositorio.ufsm.br/bitstream/1/12449/1/TES_PPGBT_2017_CRUZ_LITIELE.pdf83ad48dddbe8a0b104806d9e2f71169bMD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8804http://repositorio.ufsm.br/bitstream/1/12449/2/license_rdfc1efe8e24d7281448e873be30ea326ffMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81956http://repositorio.ufsm.br/bitstream/1/12449/3/license.txt2f0571ecee68693bd5cd3f17c1e075dfMD53TEXTTES_PPGBT_2017_CRUZ_LITIELE.pdf.txtTES_PPGBT_2017_CRUZ_LITIELE.pdf.txtExtracted texttext/plain115667http://repositorio.ufsm.br/bitstream/1/12449/4/TES_PPGBT_2017_CRUZ_LITIELE.pdf.txt7673d58521bcbceff78669996e08a6acMD54THUMBNAILTES_PPGBT_2017_CRUZ_LITIELE.pdf.jpgTES_PPGBT_2017_CRUZ_LITIELE.pdf.jpgIM Thumbnailimage/jpeg4441http://repositorio.ufsm.br/bitstream/1/12449/5/TES_PPGBT_2017_CRUZ_LITIELE.pdf.jpge61537c0557f96d183ba0ae5b112d99dMD551/124492018-02-16 09:39:11.589oai:repositorio.ufsm.br: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 Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2018-02-16T11:39:11Biblioteca Digital de Teses e Dissertações do UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Consumo de mel fornece proteção contra alterações motoras e moleculares induzidas por hipóxia/reperfusão em Drosophila melanogaster |
dc.title.alternative.eng.fl_str_mv |
Honey consumption provides protection against motor and molecular changes induced by hypoxia / reperfusion in Drosophila melanogaster |
title |
Consumo de mel fornece proteção contra alterações motoras e moleculares induzidas por hipóxia/reperfusão em Drosophila melanogaster |
spellingShingle |
Consumo de mel fornece proteção contra alterações motoras e moleculares induzidas por hipóxia/reperfusão em Drosophila melanogaster Cruz, Litiele Cezar da Mel Antioxidante Hipoxia/reperfusão Drosophila melanogaster Estresse oxidativo Honey Antioxidant Hypoxia/reperfusion Oxidative stress CNPQ::CIENCIAS BIOLOGICAS |
title_short |
Consumo de mel fornece proteção contra alterações motoras e moleculares induzidas por hipóxia/reperfusão em Drosophila melanogaster |
title_full |
Consumo de mel fornece proteção contra alterações motoras e moleculares induzidas por hipóxia/reperfusão em Drosophila melanogaster |
title_fullStr |
Consumo de mel fornece proteção contra alterações motoras e moleculares induzidas por hipóxia/reperfusão em Drosophila melanogaster |
title_full_unstemmed |
Consumo de mel fornece proteção contra alterações motoras e moleculares induzidas por hipóxia/reperfusão em Drosophila melanogaster |
title_sort |
Consumo de mel fornece proteção contra alterações motoras e moleculares induzidas por hipóxia/reperfusão em Drosophila melanogaster |
author |
Cruz, Litiele Cezar da |
author_facet |
Cruz, Litiele Cezar da |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Franco, Jeferson Luis |
dc.contributor.advisor2.fl_str_mv |
Fachinetto, Roselei |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/1680065573338339 |
dc.contributor.advisor2Lattes.fl_str_mv |
http://lattes.cnpq.br/7203076675431306 |
dc.contributor.referee1.fl_str_mv |
Fachinetto, Roselei |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/7203076675431306 |
dc.contributor.referee2.fl_str_mv |
Dalla Corte, Cristiane Lenz |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/5296284169605317 |
dc.contributor.referee3.fl_str_mv |
Puntel, Robson Luiz |
dc.contributor.referee3Lattes.fl_str_mv |
http://lattes.cnpq.br/1134532326779900 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/2555769571752143 |
dc.contributor.author.fl_str_mv |
Cruz, Litiele Cezar da |
contributor_str_mv |
Franco, Jeferson Luis Fachinetto, Roselei Fachinetto, Roselei Dalla Corte, Cristiane Lenz Puntel, Robson Luiz |
dc.subject.por.fl_str_mv |
Mel Antioxidante Hipoxia/reperfusão Drosophila melanogaster Estresse oxidativo |
topic |
Mel Antioxidante Hipoxia/reperfusão Drosophila melanogaster Estresse oxidativo Honey Antioxidant Hypoxia/reperfusion Oxidative stress CNPQ::CIENCIAS BIOLOGICAS |
dc.subject.eng.fl_str_mv |
Honey Antioxidant Hypoxia/reperfusion Oxidative stress |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS |
description |
Honey is a complex product produced by honeybees from nectar, being widely used for its sweetening properties, as well as for its benefits to human health. Honey presents anti-inflammatory, hypoglycemic and antioxidant properties. Hypoxia followed by reperfusion is a condition triggered in several pathologies and can culminate with oxidative tissue damage. The fruit-fly, Drosophila melanogaster, has hypoxia tolerant characteristics and homology with vertebrate from pathways involved in the cascade of cellular responses for hypoxia/reperfusion. Considering these aspects, the main objective of this work was to evaluate the possible protective effect of honey produced in the Brazilian Pampa Biome on changes induced by hypoxia/reperfusion in Drosophila melanogaster, with emphasis on oxidative stress. Wild flies were treated with a diet containing 10% honey for 3 days and subsequently submitted to hypoxia conditions (3hs). After reperfusion of oxygen, parameters of survival, behavior, oxidative stress as well as mRNA expression of genes related with metabolic, inflammation and death pathways were evaluated. The results demonstrated that the honey tested is composed of compounds with antioxidant activity that were recognized both in vitro and in vivo. Hypoxia/reperfusion induced an increase in the regulation of mRNA expression of Sima (HIF-1), NFκB, Nrf2, HOX, AKT-1, INR, dILP2, dILP5 and HSP27. Indeed, flies submitted to hypoxia/reperfusion has a expression decreased of EWG and Opa-1. Honey was able to reestablish the expression of Opa-1, a gene related to mitochondrial fusion. Honey treatment also reduced the changes induced by hypoxia/reperfusion in parameters of survival, locomotor deficits, wing position, reactive oxygen species (ROS) production, GST, caspases and mitochondrial complex I activities. The results obtained here show, for the first time, the beneficial effects of honey against changes induced by hypoxia/reperfusion in Drosophila melanogaster. This study evidenced that that the honey from the Brazilian Pampa Biome has a high quality and a significant antioxidant activity in vivo, which can be related with its beneficial effects against hypoxia/reperfusion. In addition, we consider the honey as well as Drosophila melanogaster promising targets for studying pathologies associated with oxidative stress and hypoxia/reperfusion. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017-02-14 |
dc.date.accessioned.fl_str_mv |
2018-02-16T11:39:11Z |
dc.date.available.fl_str_mv |
2018-02-16T11:39:11Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/12449 |
url |
http://repositorio.ufsm.br/handle/1/12449 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.cnpq.fl_str_mv |
200000000006 |
dc.relation.confidence.fl_str_mv |
600 |
dc.relation.authority.fl_str_mv |
16ea12f1-12c6-4435-a98c-cdeb5f7d1c59 7c74148e-291c-43ef-b5b6-8d69e2b8ca29 d4a64262-0e7e-45c5-910e-0b0aee907c10 ed6a73a6-ab4c-4b6e-ac2a-ec6b5c7c040b e5162be2-b3cb-46b9-9e5a-bea0d2c8bc48 |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Naturais e Exatas |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Ciências Biológicas |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Naturais e Exatas |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações do UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Biblioteca Digital de Teses e Dissertações do UFSM |
collection |
Biblioteca Digital de Teses e Dissertações do UFSM |
bitstream.url.fl_str_mv |
http://repositorio.ufsm.br/bitstream/1/12449/1/TES_PPGBT_2017_CRUZ_LITIELE.pdf http://repositorio.ufsm.br/bitstream/1/12449/2/license_rdf http://repositorio.ufsm.br/bitstream/1/12449/3/license.txt http://repositorio.ufsm.br/bitstream/1/12449/4/TES_PPGBT_2017_CRUZ_LITIELE.pdf.txt http://repositorio.ufsm.br/bitstream/1/12449/5/TES_PPGBT_2017_CRUZ_LITIELE.pdf.jpg |
bitstream.checksum.fl_str_mv |
83ad48dddbe8a0b104806d9e2f71169b c1efe8e24d7281448e873be30ea326ff 2f0571ecee68693bd5cd3f17c1e075df 7673d58521bcbceff78669996e08a6ac e61537c0557f96d183ba0ae5b112d99d |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 MD5 |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações do UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
_version_ |
1793240107405279232 |