Avaliação da atividade antioxidante de diferentes classes de compostos contra a oxidação de lipoproteínas de baixa densidade
Ano de defesa: | 2011 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , , , |
Tipo de documento: | Tese |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Santa Maria
|
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica
|
Departamento: |
Bioquímica
|
País: |
BR
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.ufsm.br/handle/1/4433 |
Resumo: | Abnormalities of lipid metabolism often lead to pathologic lipid accumulation in the vessel wall, oxidative and chronic inflammatory sequelae and the formation of atherosclerotic lesions, ultimately leading to clinical events. Low density lipoprotein (LDL) oxidative modification in the vascular wall seems to be a key factor in atherosclerosis development. Following the oxidation hypothesis of atherosclerosis the role of antioxidants has been investigated in a large number of epidemiological, clinical and experimental studies. Therefore, we investigated the antioxidant activity of three compounds of different classes in preventing the low density lipoprotein oxidation. In the guaraná study, we showed that subjects who habitually ingested guaraná (GI) demonstrated lower conjugated dienes production than did subjects who never ingested guaraná (NG; reduction of 27%, p < 0,0014), independent of other variables. However, in the GI group the conjugated dienes production was positively associated with glucose levels. The GI group also showed a total cholesterol level significantly lower than NG group. Also, guaraná demonstrated a high antioxidant activity in vitro, mainly at concentrations of 1 and 5 μg/mL, demonstrated by suppression of CDs and TBARS productions, tryptophan destruction and high TRAP activity. Guaraná, similar to other foods rich in caffeine and catechins such as green tea, has some effect on LDL oxidation that could partially explain the protective effects of this food in cardiometabolic diseases. In the thiosemicarbazone study, salicylaldehyde-4- phenylthiosemicarbazone (SPTS) may have antioxidant activity against Cu2+- and 2,2′- Azobis(2-methylpropionamidine) dihydrochloride (AAPH)-induced LDL and serum oxidation. Additionally, SPTS was effective at preventing tryptophan destruction. SPTS also showed significant total radical-trapping antioxidant activity and could prevent thiobarbituric acid reactive substances (TBARS) formation induced by sodium nitroprusside in different rat tissues and by Cu2+ in human LDL and serum. These results indicate that the antioxidant effect of SPTS is caused by a combination of transition metal chelation and free-radicalscavenging activity. In the organotellurium study, the 2-phenyl-2-tellurophenyl vinylphosphonate (DPTVP) may have antioxidant activity against Cu2+- and 2,2′-azobis(2- methylpropionamidine) dihydrochloride (AAPH)-induced LDL and serum oxidation. Additionally, DPTVP was effective at preventing tryptophan oxidation. DPTVP also showed significant total radical-trapping antioxidant activity and could prevent thiobarbituric acid reactive substance (TBARS) formation induced by Cu2+ in human LDL and serum. Additionally, DPTVP exhibited no toxicity in rat aorta slices. The results presented here indicate that the antioxidant effect of DPTVP is caused by a combination of free-radicalscavenging activity and possible blockade of the copper binding sites of LDL. Considering these preliminary results, we can conclude that the three compounds presented a potential antioxidant activity and could prevent the oxidative modifications of LDL. These data encourage us to evaluate these compounds in in vivo studies and investigate additional properties in preventing the atherogenic process. |
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2017-04-262017-04-262011-10-04PORTELLA, Rafael de Lima. Evaluation of antioxidant activity of different classes of compounds against low density lipoprotein oxidation. 2011. 147 f. Tese (Doutorado em Bioquímica) - Universidade Federal de Santa Maria, Santa Maria, 2011.http://repositorio.ufsm.br/handle/1/4433Abnormalities of lipid metabolism often lead to pathologic lipid accumulation in the vessel wall, oxidative and chronic inflammatory sequelae and the formation of atherosclerotic lesions, ultimately leading to clinical events. Low density lipoprotein (LDL) oxidative modification in the vascular wall seems to be a key factor in atherosclerosis development. Following the oxidation hypothesis of atherosclerosis the role of antioxidants has been investigated in a large number of epidemiological, clinical and experimental studies. Therefore, we investigated the antioxidant activity of three compounds of different classes in preventing the low density lipoprotein oxidation. In the guaraná study, we showed that subjects who habitually ingested guaraná (GI) demonstrated lower conjugated dienes production than did subjects who never ingested guaraná (NG; reduction of 27%, p < 0,0014), independent of other variables. However, in the GI group the conjugated dienes production was positively associated with glucose levels. The GI group also showed a total cholesterol level significantly lower than NG group. Also, guaraná demonstrated a high antioxidant activity in vitro, mainly at concentrations of 1 and 5 μg/mL, demonstrated by suppression of CDs and TBARS productions, tryptophan destruction and high TRAP activity. Guaraná, similar to other foods rich in caffeine and catechins such as green tea, has some effect on LDL oxidation that could partially explain the protective effects of this food in cardiometabolic diseases. In the thiosemicarbazone study, salicylaldehyde-4- phenylthiosemicarbazone (SPTS) may have antioxidant activity against Cu2+- and 2,2′- Azobis(2-methylpropionamidine) dihydrochloride (AAPH)-induced LDL and serum oxidation. Additionally, SPTS was effective at preventing tryptophan destruction. SPTS also showed significant total radical-trapping antioxidant activity and could prevent thiobarbituric acid reactive substances (TBARS) formation induced by sodium nitroprusside in different rat tissues and by Cu2+ in human LDL and serum. These results indicate that the antioxidant effect of SPTS is caused by a combination of transition metal chelation and free-radicalscavenging activity. In the organotellurium study, the 2-phenyl-2-tellurophenyl vinylphosphonate (DPTVP) may have antioxidant activity against Cu2+- and 2,2′-azobis(2- methylpropionamidine) dihydrochloride (AAPH)-induced LDL and serum oxidation. Additionally, DPTVP was effective at preventing tryptophan oxidation. DPTVP also showed significant total radical-trapping antioxidant activity and could prevent thiobarbituric acid reactive substance (TBARS) formation induced by Cu2+ in human LDL and serum. Additionally, DPTVP exhibited no toxicity in rat aorta slices. The results presented here indicate that the antioxidant effect of DPTVP is caused by a combination of free-radicalscavenging activity and possible blockade of the copper binding sites of LDL. Considering these preliminary results, we can conclude that the three compounds presented a potential antioxidant activity and could prevent the oxidative modifications of LDL. These data encourage us to evaluate these compounds in in vivo studies and investigate additional properties in preventing the atherogenic process.Anormalidades do metabolismo lipídico muitas vezes levam ao acúmulo patológico de lipídios na parede arterial, sequelas oxidativas e inflamatórias crônicas e à formação de lesões ateroscleróticas, levando a eventos clínicos. A modificação oxidativa de lipoproteínas de baixa densidade (LDL) na parede arterial parece ser um fator importante no desenvolvimento da aterosclerose. Seguindo a hipótese oxidativa da aterosclerose, o papel dos antioxidantes tem sido investigado em grande número de estudos epidemiológicos, clínicos e experimentais. Sendo assim, neste estudo nós investigamos a atividade antioxidante de três compostos de diferentes classes na prevenção da oxidação da LDL. No estudo com guaraná, nós mostramos que indivíduos que consumiam guaraná habitualmente (GI) apresentaram menor produção de dienos conjugados que os indivíduos que nunca consumiam guaraná (NG; redução de 27%, p < 0,0014), independentemente de outras variáveis. No entanto, no grupo GI a produção de dienos conjugados foi positivamente associada com os níveis de glicose. O grupo GI também apresentou nível de colesterol total significativamente menor comparado ao grupo NG. Além disso, o guaraná apresentou uma grande atividade antioxidante in vitro, principalmente nas concentrações de 1 e 5 μg/mL, demonstrado pela supressão da produção de dienos conjugados e substâncias reativas ao ácido tiobarbitúrico, prevenção da destruição do triptofano e alta atividade scavenger de radicais (TRAP). O guaraná, similar a outros alimentos ricos em cafeína e catequinas como o chá verde, tem alguns efeitos na oxidação da LDL que podem explicar parcialmente os efeitos protetores deste alimento nas doenças cardiometabólicas. No estudo com a tiosemicarbazona, a salicilaldeído-4-feniltiosemicarbazona (SPTS) apresentou atividade antioxidante contra a oxidação de LDL e soro induzidas por Cu2+ e 2,2 -azobis(2- metilpropionamidina) dihidrocloreto (AAPH). Além disso, a SPTS foi efetiva em prevenir a destruição do triptofano. O composto também apresentou significativa atividade scavenger de radicais e pode prevenir a formação de substâncias reativas ao ácido tiobarbitúrico induzidas por nitroprussiato de sódio em diferentes tecidos de ratos e por Cu2+ em LDL e soro humano. Estes resultados indicam que o efeito antioxidante da SPTS é causado pela combinação da atividade quelante do composto e a atividade scavenger de radicais livres. No estudo com o composto orgânico de telúrio, o 2-fenil-2-telurofenil vinilfosfonato (DPTVP) apresentou atividade antioxidante contra a oxidação de LDL e soro humano induzida por AAPH e Cu2+. Além disso, o composto preveniu a oxidação do triptofano e a formação de substâncias reativas ao ácido tiobarbitúrico e mostrou um significativo efeito scavenger de radicais. O DPTVP (20 μM) não apresentou toxicidade quando exposto à fatias de aorta de ratos. Estes resultados indicam que o efeito do DPTVP é resultado de uma combinação da atividade scavenger de radicais do composto e da possibilidade dele bloquear os sítios de ligação de cobre da LDL. Considerando todos os resultados apresentados aqui, podemos concluir que os três compostos apresentam um grande potencial antioxidante e podem prevenir as modificações oxidativas da LDL. Esses dados nos encorajam para avaliar esses compostos em estudos in vivo e investigar novas propriedades que possam prevenir o processo aterogênico.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em Ciências Biológicas: Bioquímica ToxicológicaUFSMBRBioquímicaLipoproteínas de baixa densidadeAteroscleroseAntioxidanteEstresse oxidativoPaullinia cupanaTiosemicarbazonaTelúrio orgânicoLow density lipoproteinAtherosclerosisAntioxidantOxidative stressPaullinia cupanaThiosemicarbazoneOrganotelluriumCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICAAvaliação da atividade antioxidante de diferentes classes de compostos contra a oxidação de lipoproteínas de baixa densidadeEvaluation of antioxidant activity of different classes of compounds against low density lipoprotein oxidationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisSoares, Félix Alexandre Antuneshttp://lattes.cnpq.br/8752453650114092Wagner, Carolinehttp://lattes.cnpq.br/4004565241849091Moresco, Rafael Noalhttp://lattes.cnpq.br/2269922709577261Brandão, Ricardohttp://lattes.cnpq.br/9154529811247631Fachinetto, Roseleihttp://lattes.cnpq.br/7203076675431306http://lattes.cnpq.br/5788123780581913Portella, Rafael de Lima2008000000024005003003003003003003aef53b7-b173-4c56-a944-bed2de5e5ddabe728a3f-1040-4aec-a31e-14e25eddc4f5580e04d6-2496-45ed-86a2-aa0657b90282265a0834-90e2-4afa-aac5-e50926346010e2b87c2a-7e82-4c98-abb2-1a6869cfa0f647851031-d698-41a7-a810-90b77c6b42e8info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações do UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALPORTELLA, RAFAEL DE LIMA.pdfapplication/pdf1421164http://repositorio.ufsm.br/bitstream/1/4433/1/PORTELLA%2c%20RAFAEL%20DE%20LIMA.pdf719f9ce9e30c2d6e825014d13f1fad9eMD51TEXTPORTELLA, RAFAEL DE LIMA.pdf.txtPORTELLA, RAFAEL DE LIMA.pdf.txtExtracted texttext/plain225777http://repositorio.ufsm.br/bitstream/1/4433/2/PORTELLA%2c%20RAFAEL%20DE%20LIMA.pdf.txt238828dce51ef6659261bcab5037d77cMD52THUMBNAILPORTELLA, RAFAEL DE LIMA.pdf.jpgPORTELLA, RAFAEL DE LIMA.pdf.jpgIM Thumbnailimage/jpeg5438http://repositorio.ufsm.br/bitstream/1/4433/3/PORTELLA%2c%20RAFAEL%20DE%20LIMA.pdf.jpg05b29d000e39ce4a29c0a625127fec14MD531/44332023-06-26 09:45:52.443oai:repositorio.ufsm.br:1/4433Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2023-06-26T12:45:52Biblioteca Digital de Teses e Dissertações do UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Avaliação da atividade antioxidante de diferentes classes de compostos contra a oxidação de lipoproteínas de baixa densidade |
dc.title.alternative.eng.fl_str_mv |
Evaluation of antioxidant activity of different classes of compounds against low density lipoprotein oxidation |
title |
Avaliação da atividade antioxidante de diferentes classes de compostos contra a oxidação de lipoproteínas de baixa densidade |
spellingShingle |
Avaliação da atividade antioxidante de diferentes classes de compostos contra a oxidação de lipoproteínas de baixa densidade Portella, Rafael de Lima Lipoproteínas de baixa densidade Aterosclerose Antioxidante Estresse oxidativo Paullinia cupana Tiosemicarbazona Telúrio orgânico Low density lipoprotein Atherosclerosis Antioxidant Oxidative stress Paullinia cupana Thiosemicarbazone Organotellurium CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
title_short |
Avaliação da atividade antioxidante de diferentes classes de compostos contra a oxidação de lipoproteínas de baixa densidade |
title_full |
Avaliação da atividade antioxidante de diferentes classes de compostos contra a oxidação de lipoproteínas de baixa densidade |
title_fullStr |
Avaliação da atividade antioxidante de diferentes classes de compostos contra a oxidação de lipoproteínas de baixa densidade |
title_full_unstemmed |
Avaliação da atividade antioxidante de diferentes classes de compostos contra a oxidação de lipoproteínas de baixa densidade |
title_sort |
Avaliação da atividade antioxidante de diferentes classes de compostos contra a oxidação de lipoproteínas de baixa densidade |
author |
Portella, Rafael de Lima |
author_facet |
Portella, Rafael de Lima |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Soares, Félix Alexandre Antunes |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/8752453650114092 |
dc.contributor.referee1.fl_str_mv |
Wagner, Caroline |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/4004565241849091 |
dc.contributor.referee2.fl_str_mv |
Moresco, Rafael Noal |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/2269922709577261 |
dc.contributor.referee3.fl_str_mv |
Brandão, Ricardo |
dc.contributor.referee3Lattes.fl_str_mv |
http://lattes.cnpq.br/9154529811247631 |
dc.contributor.referee4.fl_str_mv |
Fachinetto, Roselei |
dc.contributor.referee4Lattes.fl_str_mv |
http://lattes.cnpq.br/7203076675431306 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/5788123780581913 |
dc.contributor.author.fl_str_mv |
Portella, Rafael de Lima |
contributor_str_mv |
Soares, Félix Alexandre Antunes Wagner, Caroline Moresco, Rafael Noal Brandão, Ricardo Fachinetto, Roselei |
dc.subject.por.fl_str_mv |
Lipoproteínas de baixa densidade Aterosclerose Antioxidante Estresse oxidativo Paullinia cupana Tiosemicarbazona Telúrio orgânico |
topic |
Lipoproteínas de baixa densidade Aterosclerose Antioxidante Estresse oxidativo Paullinia cupana Tiosemicarbazona Telúrio orgânico Low density lipoprotein Atherosclerosis Antioxidant Oxidative stress Paullinia cupana Thiosemicarbazone Organotellurium CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
dc.subject.eng.fl_str_mv |
Low density lipoprotein Atherosclerosis Antioxidant Oxidative stress Paullinia cupana Thiosemicarbazone Organotellurium |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
description |
Abnormalities of lipid metabolism often lead to pathologic lipid accumulation in the vessel wall, oxidative and chronic inflammatory sequelae and the formation of atherosclerotic lesions, ultimately leading to clinical events. Low density lipoprotein (LDL) oxidative modification in the vascular wall seems to be a key factor in atherosclerosis development. Following the oxidation hypothesis of atherosclerosis the role of antioxidants has been investigated in a large number of epidemiological, clinical and experimental studies. Therefore, we investigated the antioxidant activity of three compounds of different classes in preventing the low density lipoprotein oxidation. In the guaraná study, we showed that subjects who habitually ingested guaraná (GI) demonstrated lower conjugated dienes production than did subjects who never ingested guaraná (NG; reduction of 27%, p < 0,0014), independent of other variables. However, in the GI group the conjugated dienes production was positively associated with glucose levels. The GI group also showed a total cholesterol level significantly lower than NG group. Also, guaraná demonstrated a high antioxidant activity in vitro, mainly at concentrations of 1 and 5 μg/mL, demonstrated by suppression of CDs and TBARS productions, tryptophan destruction and high TRAP activity. Guaraná, similar to other foods rich in caffeine and catechins such as green tea, has some effect on LDL oxidation that could partially explain the protective effects of this food in cardiometabolic diseases. In the thiosemicarbazone study, salicylaldehyde-4- phenylthiosemicarbazone (SPTS) may have antioxidant activity against Cu2+- and 2,2′- Azobis(2-methylpropionamidine) dihydrochloride (AAPH)-induced LDL and serum oxidation. Additionally, SPTS was effective at preventing tryptophan destruction. SPTS also showed significant total radical-trapping antioxidant activity and could prevent thiobarbituric acid reactive substances (TBARS) formation induced by sodium nitroprusside in different rat tissues and by Cu2+ in human LDL and serum. These results indicate that the antioxidant effect of SPTS is caused by a combination of transition metal chelation and free-radicalscavenging activity. In the organotellurium study, the 2-phenyl-2-tellurophenyl vinylphosphonate (DPTVP) may have antioxidant activity against Cu2+- and 2,2′-azobis(2- methylpropionamidine) dihydrochloride (AAPH)-induced LDL and serum oxidation. Additionally, DPTVP was effective at preventing tryptophan oxidation. DPTVP also showed significant total radical-trapping antioxidant activity and could prevent thiobarbituric acid reactive substance (TBARS) formation induced by Cu2+ in human LDL and serum. Additionally, DPTVP exhibited no toxicity in rat aorta slices. The results presented here indicate that the antioxidant effect of DPTVP is caused by a combination of free-radicalscavenging activity and possible blockade of the copper binding sites of LDL. Considering these preliminary results, we can conclude that the three compounds presented a potential antioxidant activity and could prevent the oxidative modifications of LDL. These data encourage us to evaluate these compounds in in vivo studies and investigate additional properties in preventing the atherogenic process. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011-10-04 |
dc.date.accessioned.fl_str_mv |
2017-04-26 |
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2017-04-26 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/doctoralThesis |
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doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
PORTELLA, Rafael de Lima. Evaluation of antioxidant activity of different classes of compounds against low density lipoprotein oxidation. 2011. 147 f. Tese (Doutorado em Bioquímica) - Universidade Federal de Santa Maria, Santa Maria, 2011. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/4433 |
identifier_str_mv |
PORTELLA, Rafael de Lima. Evaluation of antioxidant activity of different classes of compounds against low density lipoprotein oxidation. 2011. 147 f. Tese (Doutorado em Bioquímica) - Universidade Federal de Santa Maria, Santa Maria, 2011. |
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http://repositorio.ufsm.br/handle/1/4433 |
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