Filogeografia de Drosophila maculifrons e Drosophila griseolineata (Diptera, Drosophilidae) na região Sul do Brasil
Ano de defesa: | 2012 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Santa Maria
|
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Biodiversidade Animal
|
Departamento: |
Ciências Biológicas
|
País: |
BR
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.ufsm.br/handle/1/5318 |
Resumo: | The Quaternary period was marked by considerable changes on climate and vegetation. In the Southern Hemisphere, glaciations were milder than in the Northern Hemisphere, however, there were temperature reductions, and the climate became drier. Thus, climate-related modifications occurred in the Atlantic forest vegetation, which contracted its distribution being substituted towards other types, such as Cerrado and Caatinga . The original Atlantic forest vegetation became restricted to moist locations with milder temperatures, called refuges, which sheltered most part of the biodiversity at this time. It is known that such paleoclimatic changes affected the population dynamics of many species, especially in the Northern Hemisphere. However, it is still not clear how much these impacts influenced the species of the Neotropical region. The two species of this study, Drosophila maculifrons and Drosophila griseolineata, belong to the guaramunu group of Drosophila, and are considered sister-species that diverged about four million years ago. However, disregarding their close phylogenetic relationships, they present some distinct ecological patterns, the first species being more generalist, and the second more restricted to forest environments. Due to this ecological heterogeneity, these two species are potential indicators of the genetic consequences caused by the climatic fluctuations of the Quaternary, especially in face of a comparative perspective. The aims of this study were to evaluate the intraspecific diversity of different D. maculifrons and D. griseolineata populations, analyze the structure of individuals and populations in these two species of the guaramunu group and identify the ecological and evolutionary forces that modeled their distribution in Southern/Southeastern Brazil. In order to do so, 114 individuals were collected along the South, Southeast and Center-west regions of Brazil and Medellin, Colombia. Modeling analysis was performed, together with phylogenetic and phylogeographic analyses, with the last based in sequences of COI (Cytochrome Oxidase Subunit I) and COII (Cytochrome Oxidase Subunit II) genes. In general, the results that inferred the distribution of the most suitable habitat for each species indicate that the two species occur in sympatry at several points, although D. maculifrons is more widely distributed than D. griseolineata, at least in the brazilian territory. According to our phylogeographic analysis, D. maculifrons presents low levels of diversity and structure for mtDNA, which could be explained by a recent populational expansion event, dated for about 20 to 30 thousand years ago. On the other hand, D. griseolineata shows moderate levels of diversity and population structure, and its populations seem to have remained stables along time, showing a pattern of isolation by distance. So, it is interesting to evaluate the ecological and/or evolutionary factors which are responsible for all this difference, and this work represents a first step towards this understanding. |
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2015-01-282015-01-282012-02-24RE, Francine Cenzi de. Phylogeography of Drosophila maculifrons and Drosophila griseolineata (Diptera, Drosophilidae) in the brazilian Southern region. 2012. 72 f. Dissertação (Mestrado em Ciencias Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2012.http://repositorio.ufsm.br/handle/1/5318The Quaternary period was marked by considerable changes on climate and vegetation. In the Southern Hemisphere, glaciations were milder than in the Northern Hemisphere, however, there were temperature reductions, and the climate became drier. Thus, climate-related modifications occurred in the Atlantic forest vegetation, which contracted its distribution being substituted towards other types, such as Cerrado and Caatinga . The original Atlantic forest vegetation became restricted to moist locations with milder temperatures, called refuges, which sheltered most part of the biodiversity at this time. It is known that such paleoclimatic changes affected the population dynamics of many species, especially in the Northern Hemisphere. However, it is still not clear how much these impacts influenced the species of the Neotropical region. The two species of this study, Drosophila maculifrons and Drosophila griseolineata, belong to the guaramunu group of Drosophila, and are considered sister-species that diverged about four million years ago. However, disregarding their close phylogenetic relationships, they present some distinct ecological patterns, the first species being more generalist, and the second more restricted to forest environments. Due to this ecological heterogeneity, these two species are potential indicators of the genetic consequences caused by the climatic fluctuations of the Quaternary, especially in face of a comparative perspective. The aims of this study were to evaluate the intraspecific diversity of different D. maculifrons and D. griseolineata populations, analyze the structure of individuals and populations in these two species of the guaramunu group and identify the ecological and evolutionary forces that modeled their distribution in Southern/Southeastern Brazil. In order to do so, 114 individuals were collected along the South, Southeast and Center-west regions of Brazil and Medellin, Colombia. Modeling analysis was performed, together with phylogenetic and phylogeographic analyses, with the last based in sequences of COI (Cytochrome Oxidase Subunit I) and COII (Cytochrome Oxidase Subunit II) genes. In general, the results that inferred the distribution of the most suitable habitat for each species indicate that the two species occur in sympatry at several points, although D. maculifrons is more widely distributed than D. griseolineata, at least in the brazilian territory. According to our phylogeographic analysis, D. maculifrons presents low levels of diversity and structure for mtDNA, which could be explained by a recent populational expansion event, dated for about 20 to 30 thousand years ago. On the other hand, D. griseolineata shows moderate levels of diversity and population structure, and its populations seem to have remained stables along time, showing a pattern of isolation by distance. So, it is interesting to evaluate the ecological and/or evolutionary factors which are responsible for all this difference, and this work represents a first step towards this understanding.O período do Quaternário foi marcado por alterações consideráveis no clima e vegetação. No Hemisfério Sul, as glaciações foram mais amenas, entretanto, houve a redução da temperatura e o clima tornou-se mais seco. Dessa forma, ocorreram contrações na distribuição da Mata Atlântica e sua substituição por outros tipos, condizentes ao clima, como o Cerrado e a Caatinga. Assim, a vegetação de Mata Atlântica ficou restrita a locais úmidos e com temperaturas mais amenas, conhecidos como refúgios, os quais abrigaram grande parte da biodiversidade neste período. Sabe-se que essas alterações paleoclimáticas influenciaram a dinâmica populacional de muitas espécies, especialmente no Hemisfério Norte, porém ainda não está claro o quanto esses impactos influenciaram as espécies de distribuição Neotropical. As duas espécies em questão, Drosophila maculifrons e D. griseolineata, pertencem ao grupo guaramunu de Drosophila e são consideradas espécies irmãs, que divergiram aproximadamente há 4 milhões de anos. Porém, apesar do grau de parentesco, elas apresentam alguns padrões ecológicos distintos, sendo a primeira mais generalista e a segunda mais restrita a ambientes florestais. Devido a essa heterogeneidade ecológica, essas duas espécies são potenciais indicadoras das conseqüências genéticas ocasionadas pelas flutuações climáticas do Quaternário, principalmente em face de uma perspectiva comparativa. Os objetivos deste trabalho foram avaliar a diversidade intra-específica de diferentes populações de D. maculifrons e D. griseolineata, analisar a estruturação de indivíduos e populações nestas duas espécies do grupo guaramunu e inferir as forças ecológicas e evolutivas que modelaram sua distribuição ao longo do sul e sudeste brasileiros. Para isso, nossa amostragem conta com 114 indivíduos distribuídos ao longo das regiões Sul, Sudeste, Centro-Oeste do Brasil e Medellin na Colômbia. Foram realizadas análises de modelagem, bem como análises filogenéticas e filogeográficas, sendo que essas duas últimas basearam-se nas sequências dos genes COI (Citocromo Oxidase Subunidade I) e COII (Citocromo Oxidase Subunidade II). De uma forma geral, os resultados que inferiram a distribuição dos habitats mais adequados para cada espécie indicam que as duas espécies apresentam diversos pontos de simpatria, embora D. maculifrons seja mais amplamente distribuída em território brasileiro. De acordo com as análises filogeográficas, D. maculifrons apresenta baixos níveis de diversidade e estruturação em nível de DNA mitocondrial, o que pode ser explicado por um evento de expansão populacional recente, datado para aproximadamente 20 a 30 mil anos. Por outro lado, D. griseolineata apresenta níveis moderados de diversidade e estruturação populacional e suas populações parecem ter se mantido estáveis ao longo do tempo, apresentando um padrão de isolamento por distância. É, pois, interessante avaliar os fatores ecológicos e/ou evolutivos responsáveis por toda essa diferença, e esta dissertação representa um primeiro passo rumo a esse entendimento.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em Biodiversidade AnimalUFSMBRCiências BiológicasFilogeografiaQuaternárioGene COIGene COIIPhylogeographyQuaternaryCNPQ::CIENCIAS BIOLOGICASFilogeografia de Drosophila maculifrons e Drosophila griseolineata (Diptera, Drosophilidae) na região Sul do BrasilPhylogeography of Drosophila maculifrons and Drosophila griseolineata (Diptera, Drosophilidae) in the brazilian Southern regioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisRobe, Lizandra Jaquelinehttp://lattes.cnpq.br/0384455492228279Loreto, Elgion Lucio da Silvahttp://lattes.cnpq.br/6493669115018157Gottschalk, Marco Silvahttp://lattes.cnpq.br/3632078197153438Gaiesky, Vera Lúcia da Silva Valentehttp://lattes.cnpq.br/6961824868832863http://lattes.cnpq.br/2684838394567935De Re, Francine Cenzi20000000000640030030030030030078dfac24-0ca4-421d-920e-c5093d53e993bf9e1200-aca5-480e-a5b0-5a32c47cfe7218ce8e8e-f74e-410f-bec6-b190ea756072cc31aae8-3415-4ffb-b1af-bace9ff3861f815261e1-92a6-4b11-9ab9-69a0ea2528d6info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações do UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALDE RE, FRANCINE CENZI.pdfapplication/pdf14508096http://repositorio.ufsm.br/bitstream/1/5318/1/DE%20RE%2c%20FRANCINE%20CENZI.pdfd98fe08fc51b15c0f2288cd8e84e274aMD51TEXTDE RE, FRANCINE CENZI.pdf.txtDE RE, FRANCINE CENZI.pdf.txtExtracted texttext/plain146810http://repositorio.ufsm.br/bitstream/1/5318/2/DE%20RE%2c%20FRANCINE%20CENZI.pdf.txt324711710b2eec652669a22678ecacddMD52THUMBNAILDE RE, FRANCINE CENZI.pdf.jpgDE RE, FRANCINE CENZI.pdf.jpgIM Thumbnailimage/jpeg4911http://repositorio.ufsm.br/bitstream/1/5318/3/DE%20RE%2c%20FRANCINE%20CENZI.pdf.jpg293d8765f0e6105aaa499e09fb8b456aMD531/53182023-05-23 14:27:51.665oai:repositorio.ufsm.br:1/5318Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2023-05-23T17:27:51Biblioteca Digital de Teses e Dissertações do UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Filogeografia de Drosophila maculifrons e Drosophila griseolineata (Diptera, Drosophilidae) na região Sul do Brasil |
dc.title.alternative.eng.fl_str_mv |
Phylogeography of Drosophila maculifrons and Drosophila griseolineata (Diptera, Drosophilidae) in the brazilian Southern region |
title |
Filogeografia de Drosophila maculifrons e Drosophila griseolineata (Diptera, Drosophilidae) na região Sul do Brasil |
spellingShingle |
Filogeografia de Drosophila maculifrons e Drosophila griseolineata (Diptera, Drosophilidae) na região Sul do Brasil De Re, Francine Cenzi Filogeografia Quaternário Gene COI Gene COII Phylogeography Quaternary CNPQ::CIENCIAS BIOLOGICAS |
title_short |
Filogeografia de Drosophila maculifrons e Drosophila griseolineata (Diptera, Drosophilidae) na região Sul do Brasil |
title_full |
Filogeografia de Drosophila maculifrons e Drosophila griseolineata (Diptera, Drosophilidae) na região Sul do Brasil |
title_fullStr |
Filogeografia de Drosophila maculifrons e Drosophila griseolineata (Diptera, Drosophilidae) na região Sul do Brasil |
title_full_unstemmed |
Filogeografia de Drosophila maculifrons e Drosophila griseolineata (Diptera, Drosophilidae) na região Sul do Brasil |
title_sort |
Filogeografia de Drosophila maculifrons e Drosophila griseolineata (Diptera, Drosophilidae) na região Sul do Brasil |
author |
De Re, Francine Cenzi |
author_facet |
De Re, Francine Cenzi |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Robe, Lizandra Jaqueline |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/0384455492228279 |
dc.contributor.advisor-co1.fl_str_mv |
Loreto, Elgion Lucio da Silva |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/6493669115018157 |
dc.contributor.referee1.fl_str_mv |
Gottschalk, Marco Silva |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/3632078197153438 |
dc.contributor.referee2.fl_str_mv |
Gaiesky, Vera Lúcia da Silva Valente |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/6961824868832863 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/2684838394567935 |
dc.contributor.author.fl_str_mv |
De Re, Francine Cenzi |
contributor_str_mv |
Robe, Lizandra Jaqueline Loreto, Elgion Lucio da Silva Gottschalk, Marco Silva Gaiesky, Vera Lúcia da Silva Valente |
dc.subject.por.fl_str_mv |
Filogeografia Quaternário Gene COI Gene COII |
topic |
Filogeografia Quaternário Gene COI Gene COII Phylogeography Quaternary CNPQ::CIENCIAS BIOLOGICAS |
dc.subject.eng.fl_str_mv |
Phylogeography Quaternary |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS |
description |
The Quaternary period was marked by considerable changes on climate and vegetation. In the Southern Hemisphere, glaciations were milder than in the Northern Hemisphere, however, there were temperature reductions, and the climate became drier. Thus, climate-related modifications occurred in the Atlantic forest vegetation, which contracted its distribution being substituted towards other types, such as Cerrado and Caatinga . The original Atlantic forest vegetation became restricted to moist locations with milder temperatures, called refuges, which sheltered most part of the biodiversity at this time. It is known that such paleoclimatic changes affected the population dynamics of many species, especially in the Northern Hemisphere. However, it is still not clear how much these impacts influenced the species of the Neotropical region. The two species of this study, Drosophila maculifrons and Drosophila griseolineata, belong to the guaramunu group of Drosophila, and are considered sister-species that diverged about four million years ago. However, disregarding their close phylogenetic relationships, they present some distinct ecological patterns, the first species being more generalist, and the second more restricted to forest environments. Due to this ecological heterogeneity, these two species are potential indicators of the genetic consequences caused by the climatic fluctuations of the Quaternary, especially in face of a comparative perspective. The aims of this study were to evaluate the intraspecific diversity of different D. maculifrons and D. griseolineata populations, analyze the structure of individuals and populations in these two species of the guaramunu group and identify the ecological and evolutionary forces that modeled their distribution in Southern/Southeastern Brazil. In order to do so, 114 individuals were collected along the South, Southeast and Center-west regions of Brazil and Medellin, Colombia. Modeling analysis was performed, together with phylogenetic and phylogeographic analyses, with the last based in sequences of COI (Cytochrome Oxidase Subunit I) and COII (Cytochrome Oxidase Subunit II) genes. In general, the results that inferred the distribution of the most suitable habitat for each species indicate that the two species occur in sympatry at several points, although D. maculifrons is more widely distributed than D. griseolineata, at least in the brazilian territory. According to our phylogeographic analysis, D. maculifrons presents low levels of diversity and structure for mtDNA, which could be explained by a recent populational expansion event, dated for about 20 to 30 thousand years ago. On the other hand, D. griseolineata shows moderate levels of diversity and population structure, and its populations seem to have remained stables along time, showing a pattern of isolation by distance. So, it is interesting to evaluate the ecological and/or evolutionary factors which are responsible for all this difference, and this work represents a first step towards this understanding. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-02-24 |
dc.date.accessioned.fl_str_mv |
2015-01-28 |
dc.date.available.fl_str_mv |
2015-01-28 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
RE, Francine Cenzi de. Phylogeography of Drosophila maculifrons and Drosophila griseolineata (Diptera, Drosophilidae) in the brazilian Southern region. 2012. 72 f. Dissertação (Mestrado em Ciencias Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2012. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/5318 |
identifier_str_mv |
RE, Francine Cenzi de. Phylogeography of Drosophila maculifrons and Drosophila griseolineata (Diptera, Drosophilidae) in the brazilian Southern region. 2012. 72 f. Dissertação (Mestrado em Ciencias Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2012. |
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http://repositorio.ufsm.br/handle/1/5318 |
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Universidade Federal de Santa Maria |
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UFSM |
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Ciências Biológicas |
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Universidade Federal de Santa Maria |
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