Citocinas pró-inflamatórias em ratos experimentalmente infectados por Trypanosoma evansi
Ano de defesa: | 2011 |
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Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Santa Maria
|
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Medicina Veterinária
|
Departamento: |
Medicina Veterinária
|
País: |
BR
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.ufsm.br/handle/1/10087 |
Resumo: | The aim of this study was to measure the levels of interferon-gamma (IFN-γ), tumor necrosis factor-alpha (TNF-α), interleukin 1 (IL-1) and interleukin 6 (IL-6) in serum of rats experimentally infected with Trypanosoma evansi and to correlate with the hematological parameters. Seventy-six rats (Wistar) were divided into two groups. Group C (control) composed of twenty-eight non-inoculated rats distributed in four subgroups with seven animals each (C3, C5, C10 and C20), which received 0.2 mL saline by intraperitoneally. The group T (infected) formed of forty-eight rats was inoculated intraperitoneally with cryopreserved blood containing 1x106 trypomastigotes per animal. These, eight animals died between 5th -7th days post-infection. The remaining animals were divided into four subgroups with ten animals (T3, T5, T10 and T20) according to parasitemia degree. The blood samples were collected by cardiac puncture at the day 3 (C3, T3), 5 (C5, T5), 10 (C10, T10) and 20 (C20, T20) post infection (pi) to perform the complete blood count and determination of IFN-γ, TNF-α, IL-1 and IL-6 levels using an ELISA quantitative sandwich. Immediately after collection the animals were euthanized. The levels of all measured cytokines increased significantly (P < 0.01) in infected animals compared to the controls. T. evansi infection in rats caused an increase in serum IFN-γ, TNF-α, IL-1 and IL-6 and this increase was observed during the whole experimental infection. In addition, the increase in the cytokine levels was concomitant and directly correlated with parasitemia and anemia development at the parasitemia peak. These results suggest a synergism between these cytokines contributing to the development of anemia and the regulation of the immune response against the parasite. |
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2017-06-062017-06-062011-02-28PAIM, Francine Chimelo. Pro- inflammatory cytokines in rats experimentally infected with Trypanosoma evansi. 2011. 50 f. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2011.http://repositorio.ufsm.br/handle/1/10087The aim of this study was to measure the levels of interferon-gamma (IFN-γ), tumor necrosis factor-alpha (TNF-α), interleukin 1 (IL-1) and interleukin 6 (IL-6) in serum of rats experimentally infected with Trypanosoma evansi and to correlate with the hematological parameters. Seventy-six rats (Wistar) were divided into two groups. Group C (control) composed of twenty-eight non-inoculated rats distributed in four subgroups with seven animals each (C3, C5, C10 and C20), which received 0.2 mL saline by intraperitoneally. The group T (infected) formed of forty-eight rats was inoculated intraperitoneally with cryopreserved blood containing 1x106 trypomastigotes per animal. These, eight animals died between 5th -7th days post-infection. The remaining animals were divided into four subgroups with ten animals (T3, T5, T10 and T20) according to parasitemia degree. The blood samples were collected by cardiac puncture at the day 3 (C3, T3), 5 (C5, T5), 10 (C10, T10) and 20 (C20, T20) post infection (pi) to perform the complete blood count and determination of IFN-γ, TNF-α, IL-1 and IL-6 levels using an ELISA quantitative sandwich. Immediately after collection the animals were euthanized. The levels of all measured cytokines increased significantly (P < 0.01) in infected animals compared to the controls. T. evansi infection in rats caused an increase in serum IFN-γ, TNF-α, IL-1 and IL-6 and this increase was observed during the whole experimental infection. In addition, the increase in the cytokine levels was concomitant and directly correlated with parasitemia and anemia development at the parasitemia peak. These results suggest a synergism between these cytokines contributing to the development of anemia and the regulation of the immune response against the parasite.O objetivo deste estudo foi avaliar os níveis séricos das citocinas pró-inflamatórias interferon-gama (INF-γ), fator de necrose tumoral-alfa (TNF-α), interleucina 1 (IL-1) e interleucina 6 (IL-6) em ratos experimentalmente infectados por Trypanosoma evansi e estabelecer uma correlação com os parâmetros hematológicos. Setenta e seis ratos (Wistar) machos foram divididos em dois grupos experimentais. O Grupo C (controle) foi composto por vinte e oito ratos não inoculados distribuídos em quatro subgrupos com sete animais cada (C3, C5, C10 e C20), que receberam 0,2 mL de solução fisiológica pela via intraperitoneal. O grupo T (infectados) formado por quarenta e oito ratos inoculados intraperitonealmente com sangue criopreservado, contendo 1x106 tripomastigotas de T. evansi por animal. Destes, oito morreram entre o 5º e 7º dia pós-infecção. Os animais restantes foram divididos em quatro subgrupos de dez animais cada (T3, T5, T10 e T20) de acordo com o grau de parasitemia. As amostras de sangue foram coletadas por punção cardíaca, nos dias 3 (C3, T3), 5 (C5, T5), 10 (C10, T10) e 20 (C20,T20) pós-infecção (pi) para a realização do hemograma e determinação dos níveis séricos de INF-γ, TNF-α, IL-1 e IL-6 pela técnica de ELISA tipo sanduíche. Imediatamente após as coletas os animais eram submetidos à eutanásia. Os níveis de citocinas pró-inflamatórias aumentaram significativamente (P<0,01) nos animais infectados em relação ao grupo controle. A infecção por T. evansi em ratos provocou um aumento nos níveis séricos de INF-γ, TNF-α, IL-1, IL-6 e esse aumento foi observado durante toda a infecção experimental. Além disso, o aumento nos níveis de citocinas foi diretamente correlacionado com a parasitemia e o desenvolvimento da anemia. Estes resultados sugerem um sinergismo entre essas citocinas contribuindo para o desenvolvimento da anemia e regulação da resposta imune contra o parasito.Conselho Nacional de Desenvolvimento Científico e Tecnológicoapplication/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em Medicina VeterináriaUFSMBRMedicina VeterináriaINF-γTNF-αIL-1RatosIL-6TrypanosomosisRatsCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIACitocinas pró-inflamatórias em ratos experimentalmente infectados por Trypanosoma evansiPro-inflammatory cytokines in rats experimentally infected with Trypanosoma evansiinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisLopes, Sonia Terezinha dos Anjoshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4794864D7Krause, Alexandrehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4721148D0González, Félix Hilario Diazhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4792725A6http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4221879Y7Paim, Francine Chimelo5005000000074005005005003000e22bc1c-35eb-49dd-915f-0d5c7bca25b7a6828101-f4f4-4b5a-9e18-7f1d6475537348b1731f-9fa9-4553-ab4a-18302b9fc514b81c8fb3-a9e6-4ed7-933c-7d5ab0a4a586info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações do UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALPAIM, FRANCINE CHIMELO.pdfapplication/pdf968237http://repositorio.ufsm.br/bitstream/1/10087/1/PAIM%2c%20FRANCINE%20CHIMELO.pdf80f83652d2199815e3d5aa8d9239dd99MD51TEXTPAIM, FRANCINE CHIMELO.pdf.txtPAIM, FRANCINE CHIMELO.pdf.txtExtracted texttext/plain81868http://repositorio.ufsm.br/bitstream/1/10087/2/PAIM%2c%20FRANCINE%20CHIMELO.pdf.txt85926249a32a0cf668f0d9344090a3baMD52THUMBNAILPAIM, FRANCINE CHIMELO.pdf.jpgPAIM, FRANCINE CHIMELO.pdf.jpgIM Thumbnailimage/jpeg4819http://repositorio.ufsm.br/bitstream/1/10087/3/PAIM%2c%20FRANCINE%20CHIMELO.pdf.jpg52bc3bf28063ce2e09c747d1ab3c6e9eMD531/100872017-07-30 06:52:14.267oai:repositorio.ufsm.br:1/10087Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2017-07-30T09:52:14Biblioteca Digital de Teses e Dissertações do UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Citocinas pró-inflamatórias em ratos experimentalmente infectados por Trypanosoma evansi |
dc.title.alternative.eng.fl_str_mv |
Pro-inflammatory cytokines in rats experimentally infected with Trypanosoma evansi |
title |
Citocinas pró-inflamatórias em ratos experimentalmente infectados por Trypanosoma evansi |
spellingShingle |
Citocinas pró-inflamatórias em ratos experimentalmente infectados por Trypanosoma evansi Paim, Francine Chimelo INF-γ TNF-α IL-1 Ratos IL-6 Trypanosomosis Rats CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Citocinas pró-inflamatórias em ratos experimentalmente infectados por Trypanosoma evansi |
title_full |
Citocinas pró-inflamatórias em ratos experimentalmente infectados por Trypanosoma evansi |
title_fullStr |
Citocinas pró-inflamatórias em ratos experimentalmente infectados por Trypanosoma evansi |
title_full_unstemmed |
Citocinas pró-inflamatórias em ratos experimentalmente infectados por Trypanosoma evansi |
title_sort |
Citocinas pró-inflamatórias em ratos experimentalmente infectados por Trypanosoma evansi |
author |
Paim, Francine Chimelo |
author_facet |
Paim, Francine Chimelo |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Lopes, Sonia Terezinha dos Anjos |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4794864D7 |
dc.contributor.referee1.fl_str_mv |
Krause, Alexandre |
dc.contributor.referee1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4721148D0 |
dc.contributor.referee2.fl_str_mv |
González, Félix Hilario Diaz |
dc.contributor.referee2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4792725A6 |
dc.contributor.authorLattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4221879Y7 |
dc.contributor.author.fl_str_mv |
Paim, Francine Chimelo |
contributor_str_mv |
Lopes, Sonia Terezinha dos Anjos Krause, Alexandre González, Félix Hilario Diaz |
dc.subject.por.fl_str_mv |
INF-γ TNF-α IL-1 Ratos IL-6 |
topic |
INF-γ TNF-α IL-1 Ratos IL-6 Trypanosomosis Rats CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
dc.subject.eng.fl_str_mv |
Trypanosomosis Rats |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
The aim of this study was to measure the levels of interferon-gamma (IFN-γ), tumor necrosis factor-alpha (TNF-α), interleukin 1 (IL-1) and interleukin 6 (IL-6) in serum of rats experimentally infected with Trypanosoma evansi and to correlate with the hematological parameters. Seventy-six rats (Wistar) were divided into two groups. Group C (control) composed of twenty-eight non-inoculated rats distributed in four subgroups with seven animals each (C3, C5, C10 and C20), which received 0.2 mL saline by intraperitoneally. The group T (infected) formed of forty-eight rats was inoculated intraperitoneally with cryopreserved blood containing 1x106 trypomastigotes per animal. These, eight animals died between 5th -7th days post-infection. The remaining animals were divided into four subgroups with ten animals (T3, T5, T10 and T20) according to parasitemia degree. The blood samples were collected by cardiac puncture at the day 3 (C3, T3), 5 (C5, T5), 10 (C10, T10) and 20 (C20, T20) post infection (pi) to perform the complete blood count and determination of IFN-γ, TNF-α, IL-1 and IL-6 levels using an ELISA quantitative sandwich. Immediately after collection the animals were euthanized. The levels of all measured cytokines increased significantly (P < 0.01) in infected animals compared to the controls. T. evansi infection in rats caused an increase in serum IFN-γ, TNF-α, IL-1 and IL-6 and this increase was observed during the whole experimental infection. In addition, the increase in the cytokine levels was concomitant and directly correlated with parasitemia and anemia development at the parasitemia peak. These results suggest a synergism between these cytokines contributing to the development of anemia and the regulation of the immune response against the parasite. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011-02-28 |
dc.date.accessioned.fl_str_mv |
2017-06-06 |
dc.date.available.fl_str_mv |
2017-06-06 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
PAIM, Francine Chimelo. Pro- inflammatory cytokines in rats experimentally infected with Trypanosoma evansi. 2011. 50 f. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2011. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/10087 |
identifier_str_mv |
PAIM, Francine Chimelo. Pro- inflammatory cytokines in rats experimentally infected with Trypanosoma evansi. 2011. 50 f. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2011. |
url |
http://repositorio.ufsm.br/handle/1/10087 |
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