Influência benéfica de diferentes tempos de exercício físico sobre a drogadição induzida por morfina em ratos: aspectos comportamentais e moleculares
Ano de defesa: | 2019 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Santa Maria
Centro de Ciências da Saúde |
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Farmacologia
|
Departamento: |
Farmacologia
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.ufsm.br/handle/1/20935 |
Resumo: | Morphine is an opioid of high clinical employment, whose analgesic properties are accompanied by euphoria and well being, thus favoring drug addiction, which constitutes a serious public health problem. Drug addiction by opioids has driven the search for new treatments, but so far, those used clinically are only palliative. Physical exercise has shown beneficial in addition. But the lack of data in the literature stimulated the development of this study, whose objective was to evaluate possible molecular and behavioral adaptations resulting from different times of physical exercise in animals exposed to morphine. Wistar rats were subdivided into sedentary, short, medium and long term exercise and submitted to swimming protocol for 0, 14, 28 and 42 days, respectively. In the last week of exercise, the animals were submitted to a paradigm of morphine-conditioned place preference (CPP) (4 mg/Kg), followed by molecular analyzes involving the dopaminergic system and glucocorticoid receptor in the nucleus accumbens and hippocampus (CEUA 9373231116- UFSM). Our results show that addition of minor preference for drug in exercised groups showed less of the transporter dopamine (DAT) immunoreactivity , dopaminergic receptors type 1 and 2 (D1R and D2R) and glucocorticoid (GR) compared to sedentary group. Our study demonstrates that physical exercise, regardless of uptime, was able to modify behavioral parameters of addition per morphine, possibly through molecular adaptations in targets related to the reward system. From this study it is possible to propose physical exercise as a therapeutic approach to be strategically included in the treatment of opioid drug addiction. |
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Biblioteca Digital de Teses e Dissertações do UFSM |
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2021-05-20T11:51:14Z2021-05-20T11:51:14Z2019-02-15http://repositorio.ufsm.br/handle/1/20935Morphine is an opioid of high clinical employment, whose analgesic properties are accompanied by euphoria and well being, thus favoring drug addiction, which constitutes a serious public health problem. Drug addiction by opioids has driven the search for new treatments, but so far, those used clinically are only palliative. Physical exercise has shown beneficial in addition. But the lack of data in the literature stimulated the development of this study, whose objective was to evaluate possible molecular and behavioral adaptations resulting from different times of physical exercise in animals exposed to morphine. Wistar rats were subdivided into sedentary, short, medium and long term exercise and submitted to swimming protocol for 0, 14, 28 and 42 days, respectively. In the last week of exercise, the animals were submitted to a paradigm of morphine-conditioned place preference (CPP) (4 mg/Kg), followed by molecular analyzes involving the dopaminergic system and glucocorticoid receptor in the nucleus accumbens and hippocampus (CEUA 9373231116- UFSM). Our results show that addition of minor preference for drug in exercised groups showed less of the transporter dopamine (DAT) immunoreactivity , dopaminergic receptors type 1 and 2 (D1R and D2R) and glucocorticoid (GR) compared to sedentary group. Our study demonstrates that physical exercise, regardless of uptime, was able to modify behavioral parameters of addition per morphine, possibly through molecular adaptations in targets related to the reward system. From this study it is possible to propose physical exercise as a therapeutic approach to be strategically included in the treatment of opioid drug addiction.A morfina é um opioide de elevado emprego clínico, cujas propriedades analgésicas são acompanhadas de euforia e bem estar, favorecendo assim a drogadição, o que configura um sério problema de saúde pública. A drogadição por opioides tem impulsionado a busca por novos tratamentos, porém, até agora, os utilizados clinicamente são apenas paliativos. O exercício físico tem mostrado benefícios na adição. Porém a carência de dados na literatura estimulou o desenvolvimento deste estudo, cujo objetivo foi avaliar possíveis adaptações moleculares e comportamentais decorrentes de diferentes tempos de exercício físico em animais expostos à morfina. Ratos Wistar foram subdivididos em sedentários, exercício de curto, médio e longo prazo e submetidos ao protocolo de natação por 0, 14, 28 e 42 dias, respectivamente. Na última semana de exercício, os animais foram submetidos ao paradigma de preferência de lugar condicionado (PLC) por morfina (4 mg/Kg), seguida de análises moleculares envolvendo o sistema dopaminérgico e receptor de glicocorticóide no nucleus accumbens e hipocampo (CEUA 9373231116-UFSM). Nossos resultados apontam, que além da menor preferência pela droga, grupos exercitados mostraram menor imunorreatividade do transportador de dopamina (DAT), dos receptores dopaminérgicos tipo 1 e 2 (D1R e D2R) e glicocorticóide (GR), em relação ao grupo sedentário. Nosso estudo demonstra que o exercício físico, independentemente do tempo de atividade, modificou parâmetros comportamentais de adição por morfina, possivelmente através de adaptações moleculares em alvos relacionados ao sistema de recompensa. A partir deste estudo é possível propor o exercício físico como uma abordagem terapêutica a ser estrategicamente incluída nos tratamentos de drogadição por opioides.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESporUniversidade Federal de Santa MariaCentro de Ciências da SaúdePrograma de Pós-Graduação em FarmacologiaUFSMBrasilFarmacologiaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessAbuso de drogasAdiçãoExercício físicoOpioideDrug abuseAdditionPhysical exerciseOpioidCNPQ::CIENCIAS DA SAUDE::FARMACIAInfluência benéfica de diferentes tempos de exercício físico sobre a drogadição induzida por morfina em ratos: aspectos comportamentais e molecularesBeneficial influence of different physical exercise time on morphine induced drug addiction in rats: behavioral and molecular aspectsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisBurger, Marilise Escobarhttp://lattes.cnpq.br/9128090974948413Barcelos, Raquel CristineXXXXXXXXXXXXXXNogueira, Cristina WayneXXXXXXXXXXXXXXXRambo, Leonardo MagnoXXXXXXXXXXXXXXhttp://lattes.cnpq.br/6401035225306207Rosa, Higor Zuquetto400300000005600cea6b067-f9a7-4654-8ec5-65f1c465c1a82b4b1be1-8f5f-4d4d-b428-b5b2bd3f427b708d775a-1a3b-4c5c-9805-57283c3c6b481e373fcc-89bd-4103-adba-676f6dcec7872344c2bb-4316-443a-b897-3a2b9ac59725reponame:Biblioteca Digital de Teses e Dissertações do UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALDIS_PPGFARMACOLOGIA_2019_ROSA_HIGOR.pdfDIS_PPGFARMACOLOGIA_2019_ROSA_HIGOR.pdfDissertação de Mestradoapplication/pdf3883874http://repositorio.ufsm.br/bitstream/1/20935/1/DIS_PPGFARMACOLOGIA_2019_ROSA_HIGOR.pdfa12b2567e95de9bf7bf75e6c38be0f98MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; 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dc.title.por.fl_str_mv |
Influência benéfica de diferentes tempos de exercício físico sobre a drogadição induzida por morfina em ratos: aspectos comportamentais e moleculares |
dc.title.alternative.eng.fl_str_mv |
Beneficial influence of different physical exercise time on morphine induced drug addiction in rats: behavioral and molecular aspects |
title |
Influência benéfica de diferentes tempos de exercício físico sobre a drogadição induzida por morfina em ratos: aspectos comportamentais e moleculares |
spellingShingle |
Influência benéfica de diferentes tempos de exercício físico sobre a drogadição induzida por morfina em ratos: aspectos comportamentais e moleculares Rosa, Higor Zuquetto Abuso de drogas Adição Exercício físico Opioide Drug abuse Addition Physical exercise Opioid CNPQ::CIENCIAS DA SAUDE::FARMACIA |
title_short |
Influência benéfica de diferentes tempos de exercício físico sobre a drogadição induzida por morfina em ratos: aspectos comportamentais e moleculares |
title_full |
Influência benéfica de diferentes tempos de exercício físico sobre a drogadição induzida por morfina em ratos: aspectos comportamentais e moleculares |
title_fullStr |
Influência benéfica de diferentes tempos de exercício físico sobre a drogadição induzida por morfina em ratos: aspectos comportamentais e moleculares |
title_full_unstemmed |
Influência benéfica de diferentes tempos de exercício físico sobre a drogadição induzida por morfina em ratos: aspectos comportamentais e moleculares |
title_sort |
Influência benéfica de diferentes tempos de exercício físico sobre a drogadição induzida por morfina em ratos: aspectos comportamentais e moleculares |
author |
Rosa, Higor Zuquetto |
author_facet |
Rosa, Higor Zuquetto |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Burger, Marilise Escobar |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/9128090974948413 |
dc.contributor.advisor-co1.fl_str_mv |
Barcelos, Raquel Cristine |
dc.contributor.advisor-co1Lattes.fl_str_mv |
XXXXXXXXXXXXXX |
dc.contributor.referee1.fl_str_mv |
Nogueira, Cristina Wayne |
dc.contributor.referee1Lattes.fl_str_mv |
XXXXXXXXXXXXXXX |
dc.contributor.referee2.fl_str_mv |
Rambo, Leonardo Magno |
dc.contributor.referee2Lattes.fl_str_mv |
XXXXXXXXXXXXXX |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/6401035225306207 |
dc.contributor.author.fl_str_mv |
Rosa, Higor Zuquetto |
contributor_str_mv |
Burger, Marilise Escobar Barcelos, Raquel Cristine Nogueira, Cristina Wayne Rambo, Leonardo Magno |
dc.subject.por.fl_str_mv |
Abuso de drogas Adição Exercício físico Opioide |
topic |
Abuso de drogas Adição Exercício físico Opioide Drug abuse Addition Physical exercise Opioid CNPQ::CIENCIAS DA SAUDE::FARMACIA |
dc.subject.eng.fl_str_mv |
Drug abuse Addition Physical exercise Opioid |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS DA SAUDE::FARMACIA |
description |
Morphine is an opioid of high clinical employment, whose analgesic properties are accompanied by euphoria and well being, thus favoring drug addiction, which constitutes a serious public health problem. Drug addiction by opioids has driven the search for new treatments, but so far, those used clinically are only palliative. Physical exercise has shown beneficial in addition. But the lack of data in the literature stimulated the development of this study, whose objective was to evaluate possible molecular and behavioral adaptations resulting from different times of physical exercise in animals exposed to morphine. Wistar rats were subdivided into sedentary, short, medium and long term exercise and submitted to swimming protocol for 0, 14, 28 and 42 days, respectively. In the last week of exercise, the animals were submitted to a paradigm of morphine-conditioned place preference (CPP) (4 mg/Kg), followed by molecular analyzes involving the dopaminergic system and glucocorticoid receptor in the nucleus accumbens and hippocampus (CEUA 9373231116- UFSM). Our results show that addition of minor preference for drug in exercised groups showed less of the transporter dopamine (DAT) immunoreactivity , dopaminergic receptors type 1 and 2 (D1R and D2R) and glucocorticoid (GR) compared to sedentary group. Our study demonstrates that physical exercise, regardless of uptime, was able to modify behavioral parameters of addition per morphine, possibly through molecular adaptations in targets related to the reward system. From this study it is possible to propose physical exercise as a therapeutic approach to be strategically included in the treatment of opioid drug addiction. |
publishDate |
2019 |
dc.date.issued.fl_str_mv |
2019-02-15 |
dc.date.accessioned.fl_str_mv |
2021-05-20T11:51:14Z |
dc.date.available.fl_str_mv |
2021-05-20T11:51:14Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
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http://repositorio.ufsm.br/handle/1/20935 |
url |
http://repositorio.ufsm.br/handle/1/20935 |
dc.language.iso.fl_str_mv |
por |
language |
por |
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400300000005 |
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600 |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências da Saúde |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Farmacologia |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Farmacologia |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências da Saúde |
dc.source.none.fl_str_mv |
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