Determinação de flúor em sementes de plantas leguminosas e oleaginosas após combustão iniciada por micro-ondas em frascos descartáveis
Ano de defesa: | 2023 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Santa Maria
Centro de Ciências Naturais e Exatas |
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Química
|
Departamento: |
Química
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.ufsm.br/handle/1/29496 |
Resumo: | In this work, the use of microwave-induced combustion in disposable vessels (MIC-DV) for fluoride determination in leguminous seeds and oilseeds (almond, peanut, hazelnut, Brazil nut, pecan nut, pea, pinto bean, chickpea, and lentil) was evaluated. The determination of fluoride in food is of great relevance since this element plays important roles in the organism. However, if ingested in excess, it can cause some adversities, like dental and bone fluorosis. Since in the literature, there are no works about the determination of fluoride after MIC-DV in food, the following parameters of this sample preparation method in leguminous seeds and oilseeds were evaluated: sample mass; type and concentration of the absorbing solution; volume of the ignition solution; purging time with oxygen; and evaluation of successive combustion cycles. The fluoride determination was performed by ion chromatography (IC) and fluoride by ion selective electrode (ISE). The microwave-induced combustion (MIC) was used to obtain the reference values. Values above the limit of quantification (LOQ) were obtained only for Brazil nut and pinto bean samples, and it was only possible to perform fluoride quantification by IC. In order to improve the LOQ of the method and be able to carry out the determination by ISE, successive combustions of the pinto bean sample were carried out and the results were in agreement with those previously obtained. The proposed method could be optimized with the following conditions: 30 mg of sample for those that form pellets and 15 mg for pastes; 5 mL total ionic strength adjustment buffer as absorbing solution; 40 μL of 10 mol L-1 NH4NO3 as combustion initiator; 10 s of purging time with oxygen (4 L min-1); possibility of carrying out up to 5 successive combustion cycles. Using 5 successive combustion cycles, it was possible to determine fluoride in Brazil nut and pinto bean samples. The accuracy of the MIC-DV/ISE method was evaluated by comparing the results obtained by MIC/IC and the results were in agreement (between 94 and 106%). Therefore, it was possible to determine fluorine concentration in solutions obtained after the MIC-DV of samples of leguminous seeds and oilseeds, and only pinto bean and Brazil nut samples presented fluorine concentration above the LOQ for samples that form pellets and for pastes, corresponding to 8.3 and 16 μg g-1, respectively. |
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2023-06-19T14:38:36Z2023-06-19T14:38:36Z2023-03-10http://repositorio.ufsm.br/handle/1/29496In this work, the use of microwave-induced combustion in disposable vessels (MIC-DV) for fluoride determination in leguminous seeds and oilseeds (almond, peanut, hazelnut, Brazil nut, pecan nut, pea, pinto bean, chickpea, and lentil) was evaluated. The determination of fluoride in food is of great relevance since this element plays important roles in the organism. However, if ingested in excess, it can cause some adversities, like dental and bone fluorosis. Since in the literature, there are no works about the determination of fluoride after MIC-DV in food, the following parameters of this sample preparation method in leguminous seeds and oilseeds were evaluated: sample mass; type and concentration of the absorbing solution; volume of the ignition solution; purging time with oxygen; and evaluation of successive combustion cycles. The fluoride determination was performed by ion chromatography (IC) and fluoride by ion selective electrode (ISE). The microwave-induced combustion (MIC) was used to obtain the reference values. Values above the limit of quantification (LOQ) were obtained only for Brazil nut and pinto bean samples, and it was only possible to perform fluoride quantification by IC. In order to improve the LOQ of the method and be able to carry out the determination by ISE, successive combustions of the pinto bean sample were carried out and the results were in agreement with those previously obtained. The proposed method could be optimized with the following conditions: 30 mg of sample for those that form pellets and 15 mg for pastes; 5 mL total ionic strength adjustment buffer as absorbing solution; 40 μL of 10 mol L-1 NH4NO3 as combustion initiator; 10 s of purging time with oxygen (4 L min-1); possibility of carrying out up to 5 successive combustion cycles. Using 5 successive combustion cycles, it was possible to determine fluoride in Brazil nut and pinto bean samples. The accuracy of the MIC-DV/ISE method was evaluated by comparing the results obtained by MIC/IC and the results were in agreement (between 94 and 106%). Therefore, it was possible to determine fluorine concentration in solutions obtained after the MIC-DV of samples of leguminous seeds and oilseeds, and only pinto bean and Brazil nut samples presented fluorine concentration above the LOQ for samples that form pellets and for pastes, corresponding to 8.3 and 16 μg g-1, respectively.Neste estudo foi avaliado o uso da combustão iniciada por micro-ondas em frascos descartáveis (MIC-DV) para a posterior determinação da concentração de flúor em sementes de plantas leguminosas e oleaginosas (amêndoa, amendoim, avelã, castanha do Pará, noz pecan, ervilha, feijão carioca, grão de bico e lentilha). A quantificação de F em alimentos é de grande relevância visto que este elemento possui funções importantes para o organismo. No entanto, se ingerido em excesso, pode causar algumas adversidades como fluorose dental e óssea. Como na literatura não há relatos sobre a determinação de flúor após MIC-DV em alimentos, os seguintes parâmetros deste método de preparo de amostra em leguminosas e oleaginosas foram avaliados: massa de amostra; tipo e concentração de solução absorvedora; volume de iniciador de combustão; tempo de purga com oxigênio; e avaliação de ciclos sucessivos de combustão. A determinação de fluoreto foi feita por cromatografia de íons (IC) e por eletrodo íon seletivo para fluoreto (ISE). O método de combustão iniciada por micro-ondas (MIC) foi utilizado para a obtenção dos valores de referência. Foram obtidos valores acima do limite de quantificação (LOQ) apenas para as amostras de castanha do Pará e feijão carioca, sendo que só foi possível realizar a quantificação de fluoreto por IC. A fim de melhorar o LOQ do método e conseguir realizar a determinação também por ISE, foram feitas combustões sucessivas da amostra de feijão carioca e os resultados foram concordantes com apenas um ciclo de combustão. O método proposto foi otimizado com as seguintes condições: 30 mg de amostra para aquelas que formam comprimidos e 15 mg para as pastas; 5 mL de tampão de ajuste da força iônica total como solução absorvedora; 40 μL de NH4NO3 10 mol L-1 como iniciador de combustão; 10 s de purga com oxigênio (4 L min-1); possibilidade de realização de até 5 ciclos sucessivos de combustão. Utilizando 5 ciclos sucessivos de combustão foi possível determinar fluoreto por ISE nas amostras de castanha do Pará e feijão carioca. A exatidão do método MIC-DV/ISE foi avaliada a partir da comparação dos resultados obtidos por MIC/IC e os resultados apresentaram concordância entre 94 e 106%. Portanto, foi possível fazer a determinação da concentração de flúor em amostras de sementes de plantas leguminosas e oleaginosas, sendo que apenas as amostras de feijão carioca e castanha do Pará apresentaram concentração de flúor acima do LOQ para amostras que formam comprimidos e para as pastas, os quais correspondem a 8,3 e 16 μg g-1, respectivamente.Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPqporUniversidade Federal de Santa MariaCentro de Ciências Naturais e ExatasPrograma de Pós-Graduação em QuímicaUFSMBrasilQuímicaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessFlúorMIC-DVLeguminosasOleaginosasPreparo de amostraISEFluorideLeguminous seedsOilseedsSample preparationCNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICADeterminação de flúor em sementes de plantas leguminosas e oleaginosas após combustão iniciada por micro-ondas em frascos descartáveisFlouride determination in leguminous seeds and oilseeds after microwave-induced combustion in disposable vesselsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisDuarte, Fábio Andreihttp://lattes.cnpq.br/3503633944419329Flores, Érico Marlon de MoraesNovo, Diogo La Rosahttp://lattes.cnpq.br/6988997272441611Crestani, Paola Carpenedo100600000000600600600600600218323ac-81fc-4a7a-93bc-4536cd06202f8a567936-0ddf-4d6c-8965-af3176aaf946bce48fa2-be48-48b8-8c70-998ba4302656340b43cd-aea7-4520-886f-4cb36dfffdc3reponame:Biblioteca Digital de Teses e Dissertações do UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMCC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8805http://repositorio.ufsm.br/bitstream/1/29496/2/license_rdf4460e5956bc1d1639be9ae6146a50347MD52ORIGINALDIS_PPGQUIMICA_2023_CRESTANI_PAOLA.pdfDIS_PPGQUIMICA_2023_CRESTANI_PAOLA.pdfDissertaçãoapplication/pdf797056http://repositorio.ufsm.br/bitstream/1/29496/1/DIS_PPGQUIMICA_2023_CRESTANI_PAOLA.pdfb24c5c0a0da40e8c86d3af14d5aa128bMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81956http://repositorio.ufsm.br/bitstream/1/29496/3/license.txt2f0571ecee68693bd5cd3f17c1e075dfMD531/294962023-06-19 11:38:36.865oai:repositorio.ufsm.br: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 Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2023-06-19T14:38:36Biblioteca Digital de Teses e Dissertações do UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Determinação de flúor em sementes de plantas leguminosas e oleaginosas após combustão iniciada por micro-ondas em frascos descartáveis |
dc.title.alternative.eng.fl_str_mv |
Flouride determination in leguminous seeds and oilseeds after microwave-induced combustion in disposable vessels |
title |
Determinação de flúor em sementes de plantas leguminosas e oleaginosas após combustão iniciada por micro-ondas em frascos descartáveis |
spellingShingle |
Determinação de flúor em sementes de plantas leguminosas e oleaginosas após combustão iniciada por micro-ondas em frascos descartáveis Crestani, Paola Carpenedo Flúor MIC-DV Leguminosas Oleaginosas Preparo de amostra ISE Fluoride Leguminous seeds Oilseeds Sample preparation CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA |
title_short |
Determinação de flúor em sementes de plantas leguminosas e oleaginosas após combustão iniciada por micro-ondas em frascos descartáveis |
title_full |
Determinação de flúor em sementes de plantas leguminosas e oleaginosas após combustão iniciada por micro-ondas em frascos descartáveis |
title_fullStr |
Determinação de flúor em sementes de plantas leguminosas e oleaginosas após combustão iniciada por micro-ondas em frascos descartáveis |
title_full_unstemmed |
Determinação de flúor em sementes de plantas leguminosas e oleaginosas após combustão iniciada por micro-ondas em frascos descartáveis |
title_sort |
Determinação de flúor em sementes de plantas leguminosas e oleaginosas após combustão iniciada por micro-ondas em frascos descartáveis |
author |
Crestani, Paola Carpenedo |
author_facet |
Crestani, Paola Carpenedo |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Duarte, Fábio Andrei |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/3503633944419329 |
dc.contributor.referee1.fl_str_mv |
Flores, Érico Marlon de Moraes |
dc.contributor.referee2.fl_str_mv |
Novo, Diogo La Rosa |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/6988997272441611 |
dc.contributor.author.fl_str_mv |
Crestani, Paola Carpenedo |
contributor_str_mv |
Duarte, Fábio Andrei Flores, Érico Marlon de Moraes Novo, Diogo La Rosa |
dc.subject.por.fl_str_mv |
Flúor MIC-DV Leguminosas Oleaginosas Preparo de amostra ISE |
topic |
Flúor MIC-DV Leguminosas Oleaginosas Preparo de amostra ISE Fluoride Leguminous seeds Oilseeds Sample preparation CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA |
dc.subject.eng.fl_str_mv |
Fluoride Leguminous seeds Oilseeds Sample preparation |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA |
description |
In this work, the use of microwave-induced combustion in disposable vessels (MIC-DV) for fluoride determination in leguminous seeds and oilseeds (almond, peanut, hazelnut, Brazil nut, pecan nut, pea, pinto bean, chickpea, and lentil) was evaluated. The determination of fluoride in food is of great relevance since this element plays important roles in the organism. However, if ingested in excess, it can cause some adversities, like dental and bone fluorosis. Since in the literature, there are no works about the determination of fluoride after MIC-DV in food, the following parameters of this sample preparation method in leguminous seeds and oilseeds were evaluated: sample mass; type and concentration of the absorbing solution; volume of the ignition solution; purging time with oxygen; and evaluation of successive combustion cycles. The fluoride determination was performed by ion chromatography (IC) and fluoride by ion selective electrode (ISE). The microwave-induced combustion (MIC) was used to obtain the reference values. Values above the limit of quantification (LOQ) were obtained only for Brazil nut and pinto bean samples, and it was only possible to perform fluoride quantification by IC. In order to improve the LOQ of the method and be able to carry out the determination by ISE, successive combustions of the pinto bean sample were carried out and the results were in agreement with those previously obtained. The proposed method could be optimized with the following conditions: 30 mg of sample for those that form pellets and 15 mg for pastes; 5 mL total ionic strength adjustment buffer as absorbing solution; 40 μL of 10 mol L-1 NH4NO3 as combustion initiator; 10 s of purging time with oxygen (4 L min-1); possibility of carrying out up to 5 successive combustion cycles. Using 5 successive combustion cycles, it was possible to determine fluoride in Brazil nut and pinto bean samples. The accuracy of the MIC-DV/ISE method was evaluated by comparing the results obtained by MIC/IC and the results were in agreement (between 94 and 106%). Therefore, it was possible to determine fluorine concentration in solutions obtained after the MIC-DV of samples of leguminous seeds and oilseeds, and only pinto bean and Brazil nut samples presented fluorine concentration above the LOQ for samples that form pellets and for pastes, corresponding to 8.3 and 16 μg g-1, respectively. |
publishDate |
2023 |
dc.date.accessioned.fl_str_mv |
2023-06-19T14:38:36Z |
dc.date.available.fl_str_mv |
2023-06-19T14:38:36Z |
dc.date.issued.fl_str_mv |
2023-03-10 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/29496 |
url |
http://repositorio.ufsm.br/handle/1/29496 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.cnpq.fl_str_mv |
100600000000 |
dc.relation.confidence.fl_str_mv |
600 600 600 600 600 |
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dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Naturais e Exatas |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Química |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Química |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Naturais e Exatas |
dc.source.none.fl_str_mv |
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