Efeito in vitro de Paullinia cupana no metabolismo oxidativo de espermatozóides humanos expostos ao cloreto de metilmercúrio
Ano de defesa: | 2012 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Santa Maria
|
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica
|
Departamento: |
Bioquímica
|
País: |
BR
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.ufsm.br/handle/1/11188 |
Resumo: | Investigations suggests that methylmercury (MeHg) toxicitiy is related to oxidative stress promotion caused by lipid peroxidation of cell membranes as well as genotoxicity. The methylmercury (MeHg) exposition causes production of adverse effects as deterioration of semen quality, testicular degeneration, and male reproductive failure. Since human sperm is a vulnerable cell investigations analyzing the antioxidant protection of plant extracts on MeHg exposition is relevant. Among Brazilian food supplements we found guaraná (Paullinia cupana, Mart.) which is rich in caffeine, other metaxanthynes and catechins. Previous studies showed that guaraná present antioxidant, antimutagenic, anticarcinogenic properties that could exert protective effect of sperm cells exposed to MeHg. Therefore, an in vitro protective role of guaraná (Paullinia cupana, Mart.) viability and oxidative stress modulation of human sperm cells exposed to methylmercury intoxication was here in investigated. The human sperm cell collected from donators were counted, aliquoted and submitted to four treatments negative control (C), MeHg exposition (MeHg, 60 μM); (3) Guaraná supplementation (Gua, 10 mg/mL) and MeHg exposition plus guaraná suplementation (MeHg-Gua). During the treatment exposition the samples were maintained at 37oC at 95% CO2 incubation. The viability of spermatozoa was analyzed by MTT reduction assay. Lipoperoxidation was estimated by determination of the thiobarbituric acid derivative of MDA (TBA-MDA) using a sensitive highperformance liquid chromatographic (HPLC), reactive oxygen species (ROS) was verified by testing with fluorimetric 2′ 7′-dichlorofluorescein diacetate (DCFH-DA) assay. Protein carbonylation and thiol were evaluated spectrophotometrically. The guaraná treatment increase the sperm viability exposed to MeHg as well as decreased the lipoperoxidation, ROS and protein carbonylation levels. Thus, we conclude that the guaraná showed in vitro protective effect of human sperm against MeHg exposition. |
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2012-11-122012-11-122012-02-14FANTINEL, Mara Rejane. Effect of Paullinia cupana in vitro oxidative metabolism human sperm exposed to chloride methylmercury. 2012. 89 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2012.http://repositorio.ufsm.br/handle/1/11188Investigations suggests that methylmercury (MeHg) toxicitiy is related to oxidative stress promotion caused by lipid peroxidation of cell membranes as well as genotoxicity. The methylmercury (MeHg) exposition causes production of adverse effects as deterioration of semen quality, testicular degeneration, and male reproductive failure. Since human sperm is a vulnerable cell investigations analyzing the antioxidant protection of plant extracts on MeHg exposition is relevant. Among Brazilian food supplements we found guaraná (Paullinia cupana, Mart.) which is rich in caffeine, other metaxanthynes and catechins. Previous studies showed that guaraná present antioxidant, antimutagenic, anticarcinogenic properties that could exert protective effect of sperm cells exposed to MeHg. Therefore, an in vitro protective role of guaraná (Paullinia cupana, Mart.) viability and oxidative stress modulation of human sperm cells exposed to methylmercury intoxication was here in investigated. The human sperm cell collected from donators were counted, aliquoted and submitted to four treatments negative control (C), MeHg exposition (MeHg, 60 μM); (3) Guaraná supplementation (Gua, 10 mg/mL) and MeHg exposition plus guaraná suplementation (MeHg-Gua). During the treatment exposition the samples were maintained at 37oC at 95% CO2 incubation. The viability of spermatozoa was analyzed by MTT reduction assay. Lipoperoxidation was estimated by determination of the thiobarbituric acid derivative of MDA (TBA-MDA) using a sensitive highperformance liquid chromatographic (HPLC), reactive oxygen species (ROS) was verified by testing with fluorimetric 2′ 7′-dichlorofluorescein diacetate (DCFH-DA) assay. Protein carbonylation and thiol were evaluated spectrophotometrically. The guaraná treatment increase the sperm viability exposed to MeHg as well as decreased the lipoperoxidation, ROS and protein carbonylation levels. Thus, we conclude that the guaraná showed in vitro protective effect of human sperm against MeHg exposition.Investigations suggests that methylmercury (MeHg) toxicitiy is related to oxidative stress promotion caused by lipid peroxidation of cell membranes as well as genotoxicity. The methylmercury (MeHg) exposition causes production of adverse effects as deterioration of semen quality, testicular degeneration, and male reproductive failure. Since human sperm is a vulnerable cell investigations analyzing the antioxidant protection of plant extracts on MeHg exposition is relevant. Among Brazilian food supplements we found guaraná (Paullinia cupana, Mart.) which is rich in caffeine, other metaxanthynes and catechins. Previous studies showed that guaraná present antioxidant, antimutagenic, anticarcinogenic properties that could exert protective effect of sperm cells exposed to MeHg. Therefore, an in vitro protective role of guaraná (Paullinia cupana, Mart.) viability and oxidative stress modulation of human sperm cells exposed to methylmercury intoxication was here in investigated. The human sperm cell collected from donators were counted, aliquoted and submitted to four treatments negative control (C), MeHg exposition (MeHg, 60 μM); (3) Guaraná supplementation (Gua, 10 mg/mL) and MeHg exposition plus guaraná suplementation (MeHg-Gua). During the treatment exposition the samples were maintained at 37oC at 95% CO2 incubation. The viability of spermatozoa was analyzed by MTT reduction assay. Lipoperoxidation was estimated by determination of the thiobarbituric acid derivative of MDA (TBA-MDA) using a sensitive highperformance liquid chromatographic (HPLC), reactive oxygen species (ROS) was verified by testing with fluorimetric 2′ 7′-dichlorofluorescein diacetate (DCFH-DA) assay. Protein carbonylation and thiol were evaluated spectrophotometrically. The guaraná treatment increase the sperm viability exposed to MeHg as well as decreased the lipoperoxidation, ROS and protein carbonylation levels. Thus, we conclude that the guaraná showed in vitro protective effect of human sperm against MeHg exposition.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em Ciências Biológicas: Bioquímica ToxicológicaUFSMBRBioquímicaMercúrioToxicidadeGuaranáEspermatozóidesInfertilidadeMercuryToxicityGuaranaSpermInfertilityCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICAEfeito in vitro de Paullinia cupana no metabolismo oxidativo de espermatozóides humanos expostos ao cloreto de metilmercúrioEffect of Paullinia cupana in vitro oxidative metabolism human sperm exposed to chloride methylmercuryinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisCruz, Ivana Beatrice Mânica dahttp://lattes.cnpq.br/3426369324110716Rocha, Maria Izabel de Ugalde Marques dahttp://lattes.cnpq.br/8282487927775392Leal, Daniela Bitencourt Rosahttp://lattes.cnpq.br/3639683273462361http://lattes.cnpq.br/5570364071621144Fantinel, Mara Rejane2008000000024005003005005000b5fcfe0-b017-4163-80e2-17c110fbcf018ef8624a-df5b-476f-b13c-56c02fa9c1e25c6b237b-091a-4ca9-a9b2-b65deb9dda8ec557ce44-0763-4865-8372-1163d96ba82binfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações do UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALFANTINEL, MARA REJANE.pdfapplication/pdf552676http://repositorio.ufsm.br/bitstream/1/11188/1/FANTINEL%2c%20MARA%20REJANE.pdfcf864a4beca5183aa47e462966a7d541MD51TEXTFANTINEL, MARA REJANE.pdf.txtFANTINEL, MARA REJANE.pdf.txtExtracted texttext/plain158165http://repositorio.ufsm.br/bitstream/1/11188/2/FANTINEL%2c%20MARA%20REJANE.pdf.txte419c8d2a832765e8f79728bcd883ea5MD52THUMBNAILFANTINEL, MARA REJANE.pdf.jpgFANTINEL, MARA REJANE.pdf.jpgIM Thumbnailimage/jpeg5525http://repositorio.ufsm.br/bitstream/1/11188/3/FANTINEL%2c%20MARA%20REJANE.pdf.jpg0ed1f218633fe6349cb643378b5b57a7MD531/111882022-06-30 12:32:18.113oai:repositorio.ufsm.br:1/11188Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-06-30T15:32:18Biblioteca Digital de Teses e Dissertações do UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Efeito in vitro de Paullinia cupana no metabolismo oxidativo de espermatozóides humanos expostos ao cloreto de metilmercúrio |
dc.title.alternative.eng.fl_str_mv |
Effect of Paullinia cupana in vitro oxidative metabolism human sperm exposed to chloride methylmercury |
title |
Efeito in vitro de Paullinia cupana no metabolismo oxidativo de espermatozóides humanos expostos ao cloreto de metilmercúrio |
spellingShingle |
Efeito in vitro de Paullinia cupana no metabolismo oxidativo de espermatozóides humanos expostos ao cloreto de metilmercúrio Fantinel, Mara Rejane Mercúrio Toxicidade Guaraná Espermatozóides Infertilidade Mercury Toxicity Guarana Sperm Infertility CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
title_short |
Efeito in vitro de Paullinia cupana no metabolismo oxidativo de espermatozóides humanos expostos ao cloreto de metilmercúrio |
title_full |
Efeito in vitro de Paullinia cupana no metabolismo oxidativo de espermatozóides humanos expostos ao cloreto de metilmercúrio |
title_fullStr |
Efeito in vitro de Paullinia cupana no metabolismo oxidativo de espermatozóides humanos expostos ao cloreto de metilmercúrio |
title_full_unstemmed |
Efeito in vitro de Paullinia cupana no metabolismo oxidativo de espermatozóides humanos expostos ao cloreto de metilmercúrio |
title_sort |
Efeito in vitro de Paullinia cupana no metabolismo oxidativo de espermatozóides humanos expostos ao cloreto de metilmercúrio |
author |
Fantinel, Mara Rejane |
author_facet |
Fantinel, Mara Rejane |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Cruz, Ivana Beatrice Mânica da |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/3426369324110716 |
dc.contributor.referee1.fl_str_mv |
Rocha, Maria Izabel de Ugalde Marques da |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/8282487927775392 |
dc.contributor.referee2.fl_str_mv |
Leal, Daniela Bitencourt Rosa |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/3639683273462361 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/5570364071621144 |
dc.contributor.author.fl_str_mv |
Fantinel, Mara Rejane |
contributor_str_mv |
Cruz, Ivana Beatrice Mânica da Rocha, Maria Izabel de Ugalde Marques da Leal, Daniela Bitencourt Rosa |
dc.subject.por.fl_str_mv |
Mercúrio Toxicidade Guaraná Espermatozóides Infertilidade |
topic |
Mercúrio Toxicidade Guaraná Espermatozóides Infertilidade Mercury Toxicity Guarana Sperm Infertility CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
dc.subject.eng.fl_str_mv |
Mercury Toxicity Guarana Sperm Infertility |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
description |
Investigations suggests that methylmercury (MeHg) toxicitiy is related to oxidative stress promotion caused by lipid peroxidation of cell membranes as well as genotoxicity. The methylmercury (MeHg) exposition causes production of adverse effects as deterioration of semen quality, testicular degeneration, and male reproductive failure. Since human sperm is a vulnerable cell investigations analyzing the antioxidant protection of plant extracts on MeHg exposition is relevant. Among Brazilian food supplements we found guaraná (Paullinia cupana, Mart.) which is rich in caffeine, other metaxanthynes and catechins. Previous studies showed that guaraná present antioxidant, antimutagenic, anticarcinogenic properties that could exert protective effect of sperm cells exposed to MeHg. Therefore, an in vitro protective role of guaraná (Paullinia cupana, Mart.) viability and oxidative stress modulation of human sperm cells exposed to methylmercury intoxication was here in investigated. The human sperm cell collected from donators were counted, aliquoted and submitted to four treatments negative control (C), MeHg exposition (MeHg, 60 μM); (3) Guaraná supplementation (Gua, 10 mg/mL) and MeHg exposition plus guaraná suplementation (MeHg-Gua). During the treatment exposition the samples were maintained at 37oC at 95% CO2 incubation. The viability of spermatozoa was analyzed by MTT reduction assay. Lipoperoxidation was estimated by determination of the thiobarbituric acid derivative of MDA (TBA-MDA) using a sensitive highperformance liquid chromatographic (HPLC), reactive oxygen species (ROS) was verified by testing with fluorimetric 2′ 7′-dichlorofluorescein diacetate (DCFH-DA) assay. Protein carbonylation and thiol were evaluated spectrophotometrically. The guaraná treatment increase the sperm viability exposed to MeHg as well as decreased the lipoperoxidation, ROS and protein carbonylation levels. Thus, we conclude that the guaraná showed in vitro protective effect of human sperm against MeHg exposition. |
publishDate |
2012 |
dc.date.accessioned.fl_str_mv |
2012-11-12 |
dc.date.available.fl_str_mv |
2012-11-12 |
dc.date.issued.fl_str_mv |
2012-02-14 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
FANTINEL, Mara Rejane. Effect of Paullinia cupana in vitro oxidative metabolism human sperm exposed to chloride methylmercury. 2012. 89 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2012. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/11188 |
identifier_str_mv |
FANTINEL, Mara Rejane. Effect of Paullinia cupana in vitro oxidative metabolism human sperm exposed to chloride methylmercury. 2012. 89 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2012. |
url |
http://repositorio.ufsm.br/handle/1/11188 |
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por |
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por |
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Universidade Federal de Santa Maria |
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UFSM |
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