Desenvolvimento de método para determinação de resíduos de agrotóxicos em azeite de oliva empregando GC-MS/MS
Ano de defesa: | 2022 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Santa Maria
Centro de Ciências Naturais e Exatas |
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Química
|
Departamento: |
Química
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.ufsm.br/handle/1/25975 |
Resumo: | In Brazil, olive oil is defined as a product obtained from the fruits of the olive tree Oleaeuropaea L. and it is already configured in the international scenario as the second largest importer of olive oil. Olive oil has been used in cooking for thousands of years. Rio Grande do Sul is the state with the highest production of olives. With the intention of improving the cultivation and the quality of the final product, it is customary to use pesticides. In Brazil, the current legislation is only for olives with an MRL of 9 pesticides. Thus, the uses of pesticides that are not allowed end up being used. Therefore, to guarantee the quality of olive oils, developing an analytical method that allows the determination of pesticides in olive oil is essential. Thus, this research developed and validated an analytical method for the determination of 79 different pesticides in olive oil, using gas chromatography coupled to tandem mass spectrometry (GC-MS/MS). The method was validated for 79 compounds, and the limit of quantification (LOQ) ranged from 25 to 100 μg kg-1 and the limit of detection (LOD) from 7.5 to 30 μg kg-1 within the acceptance range used. which was 70 to 120% with RSD <20%. The method was applied to 18 samples of olive oil. Of these, 7 olive oil samples showed pesticide residues of different classes and characteristics at concentrations ranging from 8.91 to 689.41 μg kg-1. Considering the results obtained, the proposed method proved to be efficient in the determination of 79 pesticides in olive oil. |
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2022-08-25T13:23:30Z2022-08-25T13:23:30Z2022-03-16http://repositorio.ufsm.br/handle/1/25975In Brazil, olive oil is defined as a product obtained from the fruits of the olive tree Oleaeuropaea L. and it is already configured in the international scenario as the second largest importer of olive oil. Olive oil has been used in cooking for thousands of years. Rio Grande do Sul is the state with the highest production of olives. With the intention of improving the cultivation and the quality of the final product, it is customary to use pesticides. In Brazil, the current legislation is only for olives with an MRL of 9 pesticides. Thus, the uses of pesticides that are not allowed end up being used. Therefore, to guarantee the quality of olive oils, developing an analytical method that allows the determination of pesticides in olive oil is essential. Thus, this research developed and validated an analytical method for the determination of 79 different pesticides in olive oil, using gas chromatography coupled to tandem mass spectrometry (GC-MS/MS). The method was validated for 79 compounds, and the limit of quantification (LOQ) ranged from 25 to 100 μg kg-1 and the limit of detection (LOD) from 7.5 to 30 μg kg-1 within the acceptance range used. which was 70 to 120% with RSD <20%. The method was applied to 18 samples of olive oil. Of these, 7 olive oil samples showed pesticide residues of different classes and characteristics at concentrations ranging from 8.91 to 689.41 μg kg-1. Considering the results obtained, the proposed method proved to be efficient in the determination of 79 pesticides in olive oil.No Brasil, o azeite de oliva é definido como um produto obtido dos frutos da oliveira Oleaeuropaea L. e já se configura no cenário internacional como o segundo maior importador do azeite. O azeite de oliva tem sido utilizado na culinária a milhares de anos. O Rio Grande do Sul é o estado com maior produção de azeitonas. Com a intenção de melhorar o cultivo e a qualidade do produto final, costuma-se empregar agrotóxicos. No Brasil, a legislação vigente é apenas para azeitonas com LMR de 9 agrotóxicos. Assim, os usos de agrotóxicos não permitidos acabam sendo utilizados. Portanto, para garantir a qualidade dos azeites de oliva, desenvolver um método analítico que permita determinar agrotóxicos em azeite de oliva é fundamental. Dessa forma, essa pesquisa desenvolveu e validou um método analítico para determinação de 79 diferentes agrotóxicos em azeite de oliva, empregando cromatografia gasosa acoplada à espectrometria de massas em série (GC-MS/MS). O método foi validado para 79 compostos, sendo que o limite de quantificação (LOQ) variou de 25 a 100 μg kg-1 e o limite de detecção (LOD) de 7,5 a 30 μg kg-1 dentro da faixa de aceitação utilizada que foi de 70 a 120% com RSD <20%. Aplicou-se o método para 18 amostras de azeite de oliva. Dessas, 7 amostras de azeite de oliva apresentaram resíduos de agrotóxicos de diferentes classes e características em concentrações que variam de 8,91 a 689,41 μg kg-1. Considerando os resultados obtidos, o método proposto mostrou-se ser eficiente na determinação de 79 agrotóxicos em azeite de oliva.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESporUniversidade Federal de Santa MariaCentro de Ciências Naturais e ExatasPrograma de Pós-Graduação em QuímicaUFSMBrasilQuímicaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessAzeitonaAzeite de olivaAgrotóxicosQuEChERSGC-MS/MSOliveOlive oilPesticidesCNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICADesenvolvimento de método para determinação de resíduos de agrotóxicos em azeite de oliva empregando GC-MS/MSDevelopment of a method for determining pesticide residues in olive oil using GC-MS/MSinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisPrestes, Osmar Damianhttp://lattes.cnpq.br/9632234955509028Rhoden, Samile MartelPeixoto, Sandra Cadorehttp://lattes.cnpq.br/3356691243341806Kuntz, Juliana Diniz de Barros100600000000600600600600600f79cc0c9-c923-4244-9c1f-c80d74674410d1e41a2e-e959-4451-847c-e7da66365e54bf43eebf-a077-4548-9082-64c5b6993dc5a756e725-e092-4a00-a474-12d73191eb38reponame:Biblioteca Digital de Teses e Dissertações do UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALDIS_PPGQUIMICA_2022_KUNTZ_JULIANA.pdfDIS_PPGQUIMICA_2022_KUNTZ_JULIANA.pdfDissertaçãoapplication/pdf3892659http://repositorio.ufsm.br/bitstream/1/25975/1/DIS_PPGQUIMICA_2022_KUNTZ_JULIANA.pdf88453a0b22b9c3d76861f3616b3b81ceMD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8805http://repositorio.ufsm.br/bitstream/1/25975/2/license_rdf4460e5956bc1d1639be9ae6146a50347MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81956http://repositorio.ufsm.br/bitstream/1/25975/3/license.txt2f0571ecee68693bd5cd3f17c1e075dfMD531/259752022-08-25 10:23:31.023oai:repositorio.ufsm.br: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 Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-08-25T13:23:31Biblioteca Digital de Teses e Dissertações do UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Desenvolvimento de método para determinação de resíduos de agrotóxicos em azeite de oliva empregando GC-MS/MS |
dc.title.alternative.eng.fl_str_mv |
Development of a method for determining pesticide residues in olive oil using GC-MS/MS |
title |
Desenvolvimento de método para determinação de resíduos de agrotóxicos em azeite de oliva empregando GC-MS/MS |
spellingShingle |
Desenvolvimento de método para determinação de resíduos de agrotóxicos em azeite de oliva empregando GC-MS/MS Kuntz, Juliana Diniz de Barros Azeitona Azeite de oliva Agrotóxicos QuEChERS GC-MS/MS Olive Olive oil Pesticides CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA |
title_short |
Desenvolvimento de método para determinação de resíduos de agrotóxicos em azeite de oliva empregando GC-MS/MS |
title_full |
Desenvolvimento de método para determinação de resíduos de agrotóxicos em azeite de oliva empregando GC-MS/MS |
title_fullStr |
Desenvolvimento de método para determinação de resíduos de agrotóxicos em azeite de oliva empregando GC-MS/MS |
title_full_unstemmed |
Desenvolvimento de método para determinação de resíduos de agrotóxicos em azeite de oliva empregando GC-MS/MS |
title_sort |
Desenvolvimento de método para determinação de resíduos de agrotóxicos em azeite de oliva empregando GC-MS/MS |
author |
Kuntz, Juliana Diniz de Barros |
author_facet |
Kuntz, Juliana Diniz de Barros |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Prestes, Osmar Damian |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/9632234955509028 |
dc.contributor.referee1.fl_str_mv |
Rhoden, Samile Martel |
dc.contributor.referee2.fl_str_mv |
Peixoto, Sandra Cadore |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/3356691243341806 |
dc.contributor.author.fl_str_mv |
Kuntz, Juliana Diniz de Barros |
contributor_str_mv |
Prestes, Osmar Damian Rhoden, Samile Martel Peixoto, Sandra Cadore |
dc.subject.por.fl_str_mv |
Azeitona Azeite de oliva Agrotóxicos QuEChERS GC-MS/MS |
topic |
Azeitona Azeite de oliva Agrotóxicos QuEChERS GC-MS/MS Olive Olive oil Pesticides CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA |
dc.subject.eng.fl_str_mv |
Olive Olive oil Pesticides |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA |
description |
In Brazil, olive oil is defined as a product obtained from the fruits of the olive tree Oleaeuropaea L. and it is already configured in the international scenario as the second largest importer of olive oil. Olive oil has been used in cooking for thousands of years. Rio Grande do Sul is the state with the highest production of olives. With the intention of improving the cultivation and the quality of the final product, it is customary to use pesticides. In Brazil, the current legislation is only for olives with an MRL of 9 pesticides. Thus, the uses of pesticides that are not allowed end up being used. Therefore, to guarantee the quality of olive oils, developing an analytical method that allows the determination of pesticides in olive oil is essential. Thus, this research developed and validated an analytical method for the determination of 79 different pesticides in olive oil, using gas chromatography coupled to tandem mass spectrometry (GC-MS/MS). The method was validated for 79 compounds, and the limit of quantification (LOQ) ranged from 25 to 100 μg kg-1 and the limit of detection (LOD) from 7.5 to 30 μg kg-1 within the acceptance range used. which was 70 to 120% with RSD <20%. The method was applied to 18 samples of olive oil. Of these, 7 olive oil samples showed pesticide residues of different classes and characteristics at concentrations ranging from 8.91 to 689.41 μg kg-1. Considering the results obtained, the proposed method proved to be efficient in the determination of 79 pesticides in olive oil. |
publishDate |
2022 |
dc.date.accessioned.fl_str_mv |
2022-08-25T13:23:30Z |
dc.date.available.fl_str_mv |
2022-08-25T13:23:30Z |
dc.date.issued.fl_str_mv |
2022-03-16 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/25975 |
url |
http://repositorio.ufsm.br/handle/1/25975 |
dc.language.iso.fl_str_mv |
por |
language |
por |
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100600000000 |
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600 600 600 600 600 |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
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openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Naturais e Exatas |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Química |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Química |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Naturais e Exatas |
dc.source.none.fl_str_mv |
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