Efeito do ácido clorogênico, cafeina e café em parâmetros bioquimicos e comportamentais em ratos diabéticos
Ano de defesa: | 2012 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Santa Maria
|
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica
|
Departamento: |
Bioquímica
|
País: |
BR
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.ufsm.br/handle/1/11207 |
Resumo: | Studies have shown that the hyperglycemia in diabetes leads to complications in cognitive, physiological and structural central nervous system (CNS) mainly linked with increased production of reactive oxygen species (ROS), which contribute to the development of chronic complications of the diabetic state. Chlorogenic acid is a phenolic compound found in coffee that presents antioxidant, neuroprotective and hypoglycemic function. In addition, caffeine is also found in high concentrations in coffee and is known for its antioxidant, neuroprotective effects and psycho-stimulant. The objective of this study was to evaluate the effect of chlorogenic acid (CGA), caffeine (CA) and coffee (CF) in acetylcholinesterase (AChE), Na+,K+-ATPase and Aminolevulinate acid dehydratase (δ-ALA-D) activities, as well as lipid peroxidation levels (TBARS) from cerebral cortex, memory and anxiety on strepzotocin (STZ)-induced diabetic rats. The animals were divided into eight groups (n=6 13): Control; Control/CGA 5 mg/kg; Control/CA 15 mg/kg; Control/CF 0.5 g/kg; Diabetic; Diabetic/CGA 5 mg/kg; Diabetic/CA 15 mg/kg e Diabetic/CF 0.5 g/kg. Two-nine days after treatment with compounds, the animals were submitted to behavioral tests (Inhibitory avoidance and elevated plus maze) and then sacrificed and the cerebral cortex was collected. Our results demonstrated that AChE activity and TBARS levels were increased in cerebral cortex, while δ-ALA-D and Na+, K+-ATPase activities were decreased in the diabetic rats when compared to the control group. Furthermore, the diabetic rats showed deficit in memory and an increase in the anxiety. There was prevention in the increase of AChE activity when diabetic rats were treated with CGA and CA when compared with untreated rats. CGA, CA and Coffee intake restored partially cerebral δ-ALA-D and Na+, K+-ATPase when compared to untreated diabetic group. Moreover, CGA prevented diabetes-induced TBARS production and improve the memory and decrease the anxiety. Our results showed that CGA, CA and coffee can modulate cerebral oxidative stress and δ-ALA-D and Na+, K+-ATPase activity in a model of diabetes type I. Consequently, we can suppose that these compounds mainly the CGA, which has the better effect, could modulate this enzymes in CNS that are altered in diabetes. |
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2013-07-052013-07-052012-08-31STEFANELLO, Naiara. Effect of chlorogenic acid, caffeine and coffee in behavior and biochemical parameters of diabetic rats. 2012. 91 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2012.http://repositorio.ufsm.br/handle/1/11207Studies have shown that the hyperglycemia in diabetes leads to complications in cognitive, physiological and structural central nervous system (CNS) mainly linked with increased production of reactive oxygen species (ROS), which contribute to the development of chronic complications of the diabetic state. Chlorogenic acid is a phenolic compound found in coffee that presents antioxidant, neuroprotective and hypoglycemic function. In addition, caffeine is also found in high concentrations in coffee and is known for its antioxidant, neuroprotective effects and psycho-stimulant. The objective of this study was to evaluate the effect of chlorogenic acid (CGA), caffeine (CA) and coffee (CF) in acetylcholinesterase (AChE), Na+,K+-ATPase and Aminolevulinate acid dehydratase (δ-ALA-D) activities, as well as lipid peroxidation levels (TBARS) from cerebral cortex, memory and anxiety on strepzotocin (STZ)-induced diabetic rats. The animals were divided into eight groups (n=6 13): Control; Control/CGA 5 mg/kg; Control/CA 15 mg/kg; Control/CF 0.5 g/kg; Diabetic; Diabetic/CGA 5 mg/kg; Diabetic/CA 15 mg/kg e Diabetic/CF 0.5 g/kg. Two-nine days after treatment with compounds, the animals were submitted to behavioral tests (Inhibitory avoidance and elevated plus maze) and then sacrificed and the cerebral cortex was collected. Our results demonstrated that AChE activity and TBARS levels were increased in cerebral cortex, while δ-ALA-D and Na+, K+-ATPase activities were decreased in the diabetic rats when compared to the control group. Furthermore, the diabetic rats showed deficit in memory and an increase in the anxiety. There was prevention in the increase of AChE activity when diabetic rats were treated with CGA and CA when compared with untreated rats. CGA, CA and Coffee intake restored partially cerebral δ-ALA-D and Na+, K+-ATPase when compared to untreated diabetic group. Moreover, CGA prevented diabetes-induced TBARS production and improve the memory and decrease the anxiety. Our results showed that CGA, CA and coffee can modulate cerebral oxidative stress and δ-ALA-D and Na+, K+-ATPase activity in a model of diabetes type I. Consequently, we can suppose that these compounds mainly the CGA, which has the better effect, could modulate this enzymes in CNS that are altered in diabetes.Estudos demonstraram que a hiperglicemia no diabetes leva a complicações cognitivas, fisiológicas e estruturais no sistema nervoso central (SNC), causadas principalmente pelo aumento da produção de espécies reativas de oxigênio (EROS), que contribuem para o desenvolvimento de complicações crônicas do estado diabético. O ácido clorogênico é um composto fenólico encontrado no café que apresenta atividade antioxidante, neuroprotetora e hipoglicemiante. Além disso, a cafeína também é encontrada em altas concentrações no café e se destaca por sua ação antioxidante, neuroprotetora e psico-estimulante. Assim, o objetivo deste estudo foi avaliar o efeito do ácido clorogênico (CGA), cafeína (CA) e café (CF) na atividade das enzimas acetilcolinesterase (AChE), Na+, K+-ATPase e δ- aminolevulinato desidratase (δ-ALA-D), assim como seus efeitos no níveis de peroxidação lipídica (TBARS) no córtex cerebral, bem como na memória e ansiedade no diabetes induzidos por estreptozotocina (STZ) 60 mg/kg em ratos. Os animais foram divididos em oito grupos: Controle; controle/CGA 5 mg/kg; Controle/CA 15 mg/kg; Controle/CF 0,5 g/kg; Diabéticos; diabético/CGA 5 mg/kg; diabético/CA 15 mg/kg e diabético/CF 0,5 g/kg. Após 29 dias de tratamento com os compostos, os animais foram submetidos a testes comportamentais (esquiva inibitória e labirinto em cruz elevada) e, em seguida, submetidos a eutanásia e o córtex cerebral retirado. Os nossos resultados demonstraram que a atividade da AChE e os níveis de TBARS foram aumentados em córtex cerebral, enquanto que a atividade da δ-ALA-D e Na+, K+-ATPase foram diminuídas em ratos diabéticos quando comparado com os ratos controles. Além disso, os ratos diabéticos mostraram déficit na memória e um aumento na ansiedade. Houve uma prevenção no aumento da atividade da AChE quando os ratos diabéticos foram tratados com CGA e CA quando comparados com os ratos não tratados. A ingestão de CGA, CA e café restauraram parcialmente a atividade da δ-ALA-D e Na+, K+-ATPase em ratos diabéticos quando comparado com o grupo diabético sem tratamento. Além disso, os níveis de TBARS foram encontrados diminuídos nos ratos diabéticos tratados com CGA. O tratamento com CGA em ratos diabéticos também demonstrou uma melhora na memória e uma diminuição na ansiedade. Os resultados mostraram que esses compostos principalmente o CGA, composto que apresentou melhores efeitos, podem modular estas enzimas no SNC, que são alterados no diabetes.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em Ciências Biológicas: Bioquímica ToxicológicaUFSMBRBioquímicaCafeínaÁcido clorogênicoCaféDiabetes mellitusMemóriaCaffeineChlorogenic acidCoffeeMemoryCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICAEfeito do ácido clorogênico, cafeina e café em parâmetros bioquimicos e comportamentais em ratos diabéticosEffect of chlorogenic acid, caffeine and coffee in behavior and biochemical parameters of diabetic ratsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisSchetinger, Maria Rosa Chitolinahttp://lattes.cnpq.br/4401319386725357Loro, Vania Luciahttp://lattes.cnpq.br/6392817606416780Brandão, Ricardohttp://lattes.cnpq.br/9154529811247631http://lattes.cnpq.br/9810793596296712Stefanello, Naiara200800000002400500300500500f2c5f39c-089e-4825-bfba-cc66b9098981eea640cc-5a2f-4958-b238-3bd773855a032e9b2b2b-1d72-4976-bf48-be44ffcb8372e6617091-34a2-401c-87dc-5d8877667a00info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações do UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALSTEFANELLO, NAIARA.pdfapplication/pdf1093617http://repositorio.ufsm.br/bitstream/1/11207/1/STEFANELLO%2c%20NAIARA.pdfba8cadae8204a905c5497c06a94276c8MD51TEXTSTEFANELLO, NAIARA.pdf.txtSTEFANELLO, NAIARA.pdf.txtExtracted texttext/plain147218http://repositorio.ufsm.br/bitstream/1/11207/2/STEFANELLO%2c%20NAIARA.pdf.txte3b2262025ef513fa2b2a5272a972622MD52THUMBNAILSTEFANELLO, NAIARA.pdf.jpgSTEFANELLO, NAIARA.pdf.jpgIM Thumbnailimage/jpeg5511http://repositorio.ufsm.br/bitstream/1/11207/3/STEFANELLO%2c%20NAIARA.pdf.jpg50ec4056294ea3166c05f1353e33ac01MD531/112072022-06-27 11:59:06.571oai:repositorio.ufsm.br:1/11207Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-06-27T14:59:06Biblioteca Digital de Teses e Dissertações do UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Efeito do ácido clorogênico, cafeina e café em parâmetros bioquimicos e comportamentais em ratos diabéticos |
dc.title.alternative.eng.fl_str_mv |
Effect of chlorogenic acid, caffeine and coffee in behavior and biochemical parameters of diabetic rats |
title |
Efeito do ácido clorogênico, cafeina e café em parâmetros bioquimicos e comportamentais em ratos diabéticos |
spellingShingle |
Efeito do ácido clorogênico, cafeina e café em parâmetros bioquimicos e comportamentais em ratos diabéticos Stefanello, Naiara Cafeína Ácido clorogênico Café Diabetes mellitus Memória Caffeine Chlorogenic acid Coffee Memory CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
title_short |
Efeito do ácido clorogênico, cafeina e café em parâmetros bioquimicos e comportamentais em ratos diabéticos |
title_full |
Efeito do ácido clorogênico, cafeina e café em parâmetros bioquimicos e comportamentais em ratos diabéticos |
title_fullStr |
Efeito do ácido clorogênico, cafeina e café em parâmetros bioquimicos e comportamentais em ratos diabéticos |
title_full_unstemmed |
Efeito do ácido clorogênico, cafeina e café em parâmetros bioquimicos e comportamentais em ratos diabéticos |
title_sort |
Efeito do ácido clorogênico, cafeina e café em parâmetros bioquimicos e comportamentais em ratos diabéticos |
author |
Stefanello, Naiara |
author_facet |
Stefanello, Naiara |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Schetinger, Maria Rosa Chitolina |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/4401319386725357 |
dc.contributor.referee1.fl_str_mv |
Loro, Vania Lucia |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/6392817606416780 |
dc.contributor.referee2.fl_str_mv |
Brandão, Ricardo |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/9154529811247631 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/9810793596296712 |
dc.contributor.author.fl_str_mv |
Stefanello, Naiara |
contributor_str_mv |
Schetinger, Maria Rosa Chitolina Loro, Vania Lucia Brandão, Ricardo |
dc.subject.por.fl_str_mv |
Cafeína Ácido clorogênico Café Diabetes mellitus Memória |
topic |
Cafeína Ácido clorogênico Café Diabetes mellitus Memória Caffeine Chlorogenic acid Coffee Memory CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
dc.subject.eng.fl_str_mv |
Caffeine Chlorogenic acid Coffee Memory |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
description |
Studies have shown that the hyperglycemia in diabetes leads to complications in cognitive, physiological and structural central nervous system (CNS) mainly linked with increased production of reactive oxygen species (ROS), which contribute to the development of chronic complications of the diabetic state. Chlorogenic acid is a phenolic compound found in coffee that presents antioxidant, neuroprotective and hypoglycemic function. In addition, caffeine is also found in high concentrations in coffee and is known for its antioxidant, neuroprotective effects and psycho-stimulant. The objective of this study was to evaluate the effect of chlorogenic acid (CGA), caffeine (CA) and coffee (CF) in acetylcholinesterase (AChE), Na+,K+-ATPase and Aminolevulinate acid dehydratase (δ-ALA-D) activities, as well as lipid peroxidation levels (TBARS) from cerebral cortex, memory and anxiety on strepzotocin (STZ)-induced diabetic rats. The animals were divided into eight groups (n=6 13): Control; Control/CGA 5 mg/kg; Control/CA 15 mg/kg; Control/CF 0.5 g/kg; Diabetic; Diabetic/CGA 5 mg/kg; Diabetic/CA 15 mg/kg e Diabetic/CF 0.5 g/kg. Two-nine days after treatment with compounds, the animals were submitted to behavioral tests (Inhibitory avoidance and elevated plus maze) and then sacrificed and the cerebral cortex was collected. Our results demonstrated that AChE activity and TBARS levels were increased in cerebral cortex, while δ-ALA-D and Na+, K+-ATPase activities were decreased in the diabetic rats when compared to the control group. Furthermore, the diabetic rats showed deficit in memory and an increase in the anxiety. There was prevention in the increase of AChE activity when diabetic rats were treated with CGA and CA when compared with untreated rats. CGA, CA and Coffee intake restored partially cerebral δ-ALA-D and Na+, K+-ATPase when compared to untreated diabetic group. Moreover, CGA prevented diabetes-induced TBARS production and improve the memory and decrease the anxiety. Our results showed that CGA, CA and coffee can modulate cerebral oxidative stress and δ-ALA-D and Na+, K+-ATPase activity in a model of diabetes type I. Consequently, we can suppose that these compounds mainly the CGA, which has the better effect, could modulate this enzymes in CNS that are altered in diabetes. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-08-31 |
dc.date.accessioned.fl_str_mv |
2013-07-05 |
dc.date.available.fl_str_mv |
2013-07-05 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
STEFANELLO, Naiara. Effect of chlorogenic acid, caffeine and coffee in behavior and biochemical parameters of diabetic rats. 2012. 91 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2012. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/11207 |
identifier_str_mv |
STEFANELLO, Naiara. Effect of chlorogenic acid, caffeine and coffee in behavior and biochemical parameters of diabetic rats. 2012. 91 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2012. |
url |
http://repositorio.ufsm.br/handle/1/11207 |
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UFSM |
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