Impacto da osteocalcina na esteroidogenese ovariana
Ano de defesa: | 2021 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Santa Maria
Centro de Ciências Rurais |
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Medicina Veterinária
|
Departamento: |
Medicina Veterinária
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.ufsm.br/handle/1/22736 |
Resumo: | Osteocalcin, known as bone gamma-carboxy glutamic acid-containing protein (BGLAP), is a small (49-amino-acid) noncollagenous protein hormone secreted by osteoblasts usually reported as a marker of bone formation. More recently, other roles for osteocalcin in insulin secretion and sensibility have been suggested, although its impact on the ovarian steroid secretion remains obscure. Therefore, the present study aims to investigate the presence of osteocalcin receptor (GPCR6A) in the granulosa and theca cells of the bovine ovary and the possible effect of osteocalcin on steroidogenic enzymes. Our results indicate, for the first time, the presence of the osteocalcin receptor GPRC6A in theca cells and testis and its absence or very low expression in the granulosa cells. After treatment with non-carboxylated osteocalcin (100ng/ml), a significant effect size on the key steroidogenic enzymes (CYP17A1, CYP11A1) and protein (STAR) was observed in the first hour (but not 24-hour) only in the presence of co- treatment with luteinizing hormone (LH). Lower doses of non-carboxylated osteocalcin (1ng/ml) were not effective to influence these enzymes. Although limited, our results demonstrate that there appears to be some action of osteocalcin in the first hour after treatment, increasing mRNA expression in key steroidogenic enzymes such as STAR, CYP11A1, and CYP17A1 synergistically with the use of LH (100 ng/ml). |
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2021-11-05T17:34:24Z2021-11-05T17:34:24Z2021-08-25http://repositorio.ufsm.br/handle/1/22736Osteocalcin, known as bone gamma-carboxy glutamic acid-containing protein (BGLAP), is a small (49-amino-acid) noncollagenous protein hormone secreted by osteoblasts usually reported as a marker of bone formation. More recently, other roles for osteocalcin in insulin secretion and sensibility have been suggested, although its impact on the ovarian steroid secretion remains obscure. Therefore, the present study aims to investigate the presence of osteocalcin receptor (GPCR6A) in the granulosa and theca cells of the bovine ovary and the possible effect of osteocalcin on steroidogenic enzymes. Our results indicate, for the first time, the presence of the osteocalcin receptor GPRC6A in theca cells and testis and its absence or very low expression in the granulosa cells. After treatment with non-carboxylated osteocalcin (100ng/ml), a significant effect size on the key steroidogenic enzymes (CYP17A1, CYP11A1) and protein (STAR) was observed in the first hour (but not 24-hour) only in the presence of co- treatment with luteinizing hormone (LH). Lower doses of non-carboxylated osteocalcin (1ng/ml) were not effective to influence these enzymes. Although limited, our results demonstrate that there appears to be some action of osteocalcin in the first hour after treatment, increasing mRNA expression in key steroidogenic enzymes such as STAR, CYP11A1, and CYP17A1 synergistically with the use of LH (100 ng/ml).A osteocalcina é conhecida como uma proteína óssea contendo ácido gama-carboxi glutâmico (BGLAP), é um pequeno (49 aminoácidos) hormônio proteico não-colágeno secretado por osteoblastos, geralmente relatado como um marcador de formação óssea. Mais recentemente, outros papéis da osteocalcina na secreção e sensibilidade à insulina foram sugeridos, embora seu impacto na secreção de esteróides ovarianos permaneça obscuro. Portanto, o presente estudo tem como objetivo investigar a presença do receptor de osteocalcina (GPCR6A) nas células da granulosa e da teca do ovário bovino e o possível efeito da osteocalcina sobre enzimas esteroidogênicas. Nossos resultados indicam, pela primeira vez, a presença do receptor osteocalcina GPRC6A nas células e testes da teca e sua ausência ou muito baixa expressão nas células da granulosa. Após o tratamento com osteocalcina não carboxilada (100ng / ml), um tamanho de efeito significativo nas principais enzimas esteroidogênicas (CYP17A1, CYP11A1) e proteína (STAR) foi observado na primeira hora (mas não 24 horas) apenas na presença de co- tratamento com hormônio luteinizante (LH). Doses mais baixas de osteocalcina não carboxilada (1 ng / ml) não foram eficazes para influenciar essas enzimas. Embora limitados, nossos resultados demonstram que parece haver alguma ação da osteocalcina na primeira hora após o tratamento, aumento a expressão de mRNA em enzimas esteroidogenicas chaves como a STAR, CYP11A1 e CYP17A1 de forma sinérgica com o uso de LH (100 ng/ml).Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESConselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPqFundação de Amparo à Pesquisa do Estado do Rio Grande do Sul - FAPERGSFundação de Amparo à Pesquisa do Estado de São Paulo - FAPESPFinanciadora de Estudos e Projetos - FinepporUniversidade Federal de Santa MariaCentro de Ciências RuraisPrograma de Pós-Graduação em Medicina VeterináriaUFSMBrasilMedicina VeterináriaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessTecaOsteocalcinaOvários bovinosEsteroidogêneseCYP17ThecaOsteocalcinBovine ovariesSteroidogenesisCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIAImpacto da osteocalcina na esteroidogenese ovarianaThe impact of osteocalcin on ovarian steroidogenesisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisComim, Fabio Vasconcelloshttp://lattes.cnpq.br/5119233991388822Antoniazzi, Alfredo Quiteshttp://lattes.cnpq.br/7258679373419746Portela Junior, Valério Valdetar MarquesPremaor, Melissa Orlandinhttp://lattes.cnpq.br/3642830819826512Spricigo, Matheus Colpo500500000007600600600600600600f3aa2aac-05c3-4630-9797-c1e5f25b311b14770a32-8ca7-49de-aed6-e18b4c2b645a42ed6e2f-195c-40bb-a4db-30c207cafdeb8052e29a-f822-42cb-97d8-92f177b97a02e54845f9-9f23-46cb-b394-215d71d7a4b2reponame:Biblioteca Digital de Teses e Dissertações do UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMCC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; 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dc.title.por.fl_str_mv |
Impacto da osteocalcina na esteroidogenese ovariana |
dc.title.alternative.eng.fl_str_mv |
The impact of osteocalcin on ovarian steroidogenesis |
title |
Impacto da osteocalcina na esteroidogenese ovariana |
spellingShingle |
Impacto da osteocalcina na esteroidogenese ovariana Spricigo, Matheus Colpo Teca Osteocalcina Ovários bovinos Esteroidogênese CYP17 Theca Osteocalcin Bovine ovaries Steroidogenesis CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Impacto da osteocalcina na esteroidogenese ovariana |
title_full |
Impacto da osteocalcina na esteroidogenese ovariana |
title_fullStr |
Impacto da osteocalcina na esteroidogenese ovariana |
title_full_unstemmed |
Impacto da osteocalcina na esteroidogenese ovariana |
title_sort |
Impacto da osteocalcina na esteroidogenese ovariana |
author |
Spricigo, Matheus Colpo |
author_facet |
Spricigo, Matheus Colpo |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Comim, Fabio Vasconcellos |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/5119233991388822 |
dc.contributor.advisor-co1.fl_str_mv |
Antoniazzi, Alfredo Quites |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/7258679373419746 |
dc.contributor.referee1.fl_str_mv |
Portela Junior, Valério Valdetar Marques |
dc.contributor.referee2.fl_str_mv |
Premaor, Melissa Orlandin |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/3642830819826512 |
dc.contributor.author.fl_str_mv |
Spricigo, Matheus Colpo |
contributor_str_mv |
Comim, Fabio Vasconcellos Antoniazzi, Alfredo Quites Portela Junior, Valério Valdetar Marques Premaor, Melissa Orlandin |
dc.subject.por.fl_str_mv |
Teca Osteocalcina Ovários bovinos Esteroidogênese CYP17 |
topic |
Teca Osteocalcina Ovários bovinos Esteroidogênese CYP17 Theca Osteocalcin Bovine ovaries Steroidogenesis CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
dc.subject.eng.fl_str_mv |
Theca Osteocalcin Bovine ovaries Steroidogenesis |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
Osteocalcin, known as bone gamma-carboxy glutamic acid-containing protein (BGLAP), is a small (49-amino-acid) noncollagenous protein hormone secreted by osteoblasts usually reported as a marker of bone formation. More recently, other roles for osteocalcin in insulin secretion and sensibility have been suggested, although its impact on the ovarian steroid secretion remains obscure. Therefore, the present study aims to investigate the presence of osteocalcin receptor (GPCR6A) in the granulosa and theca cells of the bovine ovary and the possible effect of osteocalcin on steroidogenic enzymes. Our results indicate, for the first time, the presence of the osteocalcin receptor GPRC6A in theca cells and testis and its absence or very low expression in the granulosa cells. After treatment with non-carboxylated osteocalcin (100ng/ml), a significant effect size on the key steroidogenic enzymes (CYP17A1, CYP11A1) and protein (STAR) was observed in the first hour (but not 24-hour) only in the presence of co- treatment with luteinizing hormone (LH). Lower doses of non-carboxylated osteocalcin (1ng/ml) were not effective to influence these enzymes. Although limited, our results demonstrate that there appears to be some action of osteocalcin in the first hour after treatment, increasing mRNA expression in key steroidogenic enzymes such as STAR, CYP11A1, and CYP17A1 synergistically with the use of LH (100 ng/ml). |
publishDate |
2021 |
dc.date.accessioned.fl_str_mv |
2021-11-05T17:34:24Z |
dc.date.available.fl_str_mv |
2021-11-05T17:34:24Z |
dc.date.issued.fl_str_mv |
2021-08-25 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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http://repositorio.ufsm.br/handle/1/22736 |
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por |
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500500000007 |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
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openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Rurais |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Medicina Veterinária |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Medicina Veterinária |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências Rurais |
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