Efeito do desbalanço genético e farmacológico do metabolismo oxidativo em indicadores de fertilidade masculina
Ano de defesa: | 2016 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , , , |
Tipo de documento: | Tese |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Santa Maria
Centro de Ciências da Saúde |
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Farmacologia
|
Departamento: |
Farmacologia
|
País: |
Brasil
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.ufsm.br/handle/1/18127 |
Resumo: | Previous studies indicate that changes in oxidative metabolism may play a role in reproductive success. Manganese-dependent superoxide dismutase enzyme (SOD2), a key in the oxidative balance, presents a genetic variation Val16Ala-SOD2 that leads to a change in its efficiency causing an imbalance between the superoxide (S) and hydrogen peroxide (HP) levels of Cells. Studies have suggested that both homozygous genotypes (AA-VV) cause an S-HP imbalance associated with the risk of various chronic diseases, and interfere with female reproductive success. Thus, the objective of this work was to investigate the effect of the genetic imbalance caused by the polymorphism Val16Ala-SOD2 and pharmacological induced by the treatment with paraquat in oxidative metabolism and in male fertility indicators. For the in vitro analysis of the impact of the S-HP imbalance caused by the pharmacological action of paraquat, a method of quantification of the levels of cfDNA in the seminal plasma was conducted by fluorimetry using the DNA Picogreen. The second protocol investigated the association between the Val16Ala- SOD2 polymorphism with changes in the male fertility parameters determined by the WHO, by evaluating the association of this polymorphism with high levels of cfDNA and markers of oxidative stress in seminal plasma. Semen samples were obtained from healthy volunteers or from surplus samples used to perform sperm in laboratory clinical analyzes, the samples were processed and analyzed in the Biogenomic Laboratory, through spectrophotometric and molecular tests. The results showed a significant association between elevated levels of cfDNA and altered fertility parameters in semen, such as: low viability, changes in motility and sperm morphology. In vitro complementary analysis confirmed that high levels of S causes oxidative stress and decrease sperm viability. These results corroborated the hypothesis that cfDNA levels could be markers of general sperm quality and oxidative stress. |
id |
UFSM_b10cea2aa3ad77ebfba1939a61e6d97a |
---|---|
oai_identifier_str |
oai:repositorio.ufsm.br:1/18127 |
network_acronym_str |
UFSM |
network_name_str |
Biblioteca Digital de Teses e Dissertações do UFSM |
repository_id_str |
|
spelling |
2019-09-03T19:15:06Z2019-09-03T19:15:06Z2016-12-07http://repositorio.ufsm.br/handle/1/18127Previous studies indicate that changes in oxidative metabolism may play a role in reproductive success. Manganese-dependent superoxide dismutase enzyme (SOD2), a key in the oxidative balance, presents a genetic variation Val16Ala-SOD2 that leads to a change in its efficiency causing an imbalance between the superoxide (S) and hydrogen peroxide (HP) levels of Cells. Studies have suggested that both homozygous genotypes (AA-VV) cause an S-HP imbalance associated with the risk of various chronic diseases, and interfere with female reproductive success. Thus, the objective of this work was to investigate the effect of the genetic imbalance caused by the polymorphism Val16Ala-SOD2 and pharmacological induced by the treatment with paraquat in oxidative metabolism and in male fertility indicators. For the in vitro analysis of the impact of the S-HP imbalance caused by the pharmacological action of paraquat, a method of quantification of the levels of cfDNA in the seminal plasma was conducted by fluorimetry using the DNA Picogreen. The second protocol investigated the association between the Val16Ala- SOD2 polymorphism with changes in the male fertility parameters determined by the WHO, by evaluating the association of this polymorphism with high levels of cfDNA and markers of oxidative stress in seminal plasma. Semen samples were obtained from healthy volunteers or from surplus samples used to perform sperm in laboratory clinical analyzes, the samples were processed and analyzed in the Biogenomic Laboratory, through spectrophotometric and molecular tests. The results showed a significant association between elevated levels of cfDNA and altered fertility parameters in semen, such as: low viability, changes in motility and sperm morphology. In vitro complementary analysis confirmed that high levels of S causes oxidative stress and decrease sperm viability. These results corroborated the hypothesis that cfDNA levels could be markers of general sperm quality and oxidative stress.Estudos prévios indicam que alterações no metabolismo oxidativo podem atuar no sucesso reprodutivo. A enzima superóxido dismutase dependente de manganês (SOD2), chave no equilíbrio oxidativo, apresenta uma variação genética Val16Ala-SOD2 que leva a uma mudança na sua eficiência causando um desequilíbrio entre os níveis de superóxido (S) e peróxido de hidrogênio (HP) das células. Estudos sugeriram que os ambos genótipos homozigoticos (AA-VV) causam um desbalanço S – HP associado ao risco de diversas doenças crônicas, além de interferir no sucesso reprodutivo feminino. Assim, o objetivo desse trabalho foi investigar o efeito do desbalanço genético causado pelo polimorfismo Val16Ala-SOD2 e farmacológico induzido pelo tratamento com paraquat no metabolismo oxidativo e em indicadores de fertilidade masculina. Para a análise in vitro do impacto do desbalanço S- HP causado por ação farmacológica do paraquat, foi conduzida a validação de um método de quantificação dos níveis de cfDNA no plasma seminal através de fluorimetria utilizando o corante DNA Picogreen. O segundo protocolo investigou a associação entre o polimorfismo Val16Ala-SOD2 com alterações nos parâmetros de fertilidade masculina determinados pela OMS, através da avaliação da associação deste polimorfismo com níveis elevados de cfDNA e de marcadores de estresse oxidativo no plasma seminal. Amostras de sêmen foram obtidas de voluntários saudáveis ou de amostras excedentes utilizadas para a realização de espermograma em laboratório de análises clinicas, as amostras foram processadas e analisadas no Laboratório Biogenômica, através de testes espectrofotométricos e moleculares. O conjunto dos resultados demonstrou associação significativa entre níveis elevados de cfDNA e parâmetros alterados de fertilidade no sêmen, como : baixa viabilidade, alterações na motilidade e morfologia espermática. Análise in vitro complementar confirmou que elevados níveis de S causam estresse oxidativo e diminuem a viabilidade espermática. O conjunto destes resultados corroborou a hipótese de que os níveis de cfDNA poderiam ser marcadores da qualidade espermática geral e estresse oxidativo.porUniversidade Federal de Santa MariaCentro de Ciências da SaúdePrograma de Pós-Graduação em FarmacologiaUFSMBrasilFarmacologiaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessInfertilidadePolimorfismo Val16Ala SOD2Qualidade espermáticaEstresse oxidativoInfertilityVal16Ala-SOD2 polymorphismSperm qualityOxidative stressCNPQ::CIENCIAS DA SAUDE::FARMACIAEfeito do desbalanço genético e farmacológico do metabolismo oxidativo em indicadores de fertilidade masculinaEffect of genetic and pharmacological disbursement of oxidative metabolism in male fertility indicatorinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisCruz, Ivana Beatrice Mânica dahttp://lattes.cnpq.br/3426369324110716Fachinetto, Roseleihttp://lattes.cnpq.br/7203076675431306Bresciani, Guilherme Bergamaschihttp://lattes.cnpq.br/1247251997170368Comim, Fabio Vasconcelloshttp://lattes.cnpq.br/5119233991388822Sagrillo, Michele Roratohttp://lattes.cnpq.br/2566285176244747http://lattes.cnpq.br/7742630278309727Costa, Felipe4003000000056000b5fcfe0-b017-4163-80e2-17c110fbcf01e2208229-12a5-4c65-b374-cbc9b69d36e7d4a64262-0e7e-45c5-910e-0b0aee907c1003f335df-7f03-44d1-8866-576fb27afbe0982f3d46-745a-4f4c-83bd-1bbe76793e5be37af8b8-3d08-474c-b996-ee17aaf6b3a8reponame:Biblioteca Digital de Teses e Dissertações do UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMCC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8805http://repositorio.ufsm.br/bitstream/1/18127/2/license_rdf4460e5956bc1d1639be9ae6146a50347MD52ORIGINALTES_PPGFARMACOLOGIA_2016_COSTA_FELIPE.pdfTES_PPGFARMACOLOGIA_2016_COSTA_FELIPE.pdfTese de Doutoradoapplication/pdf885486http://repositorio.ufsm.br/bitstream/1/18127/1/TES_PPGFARMACOLOGIA_2016_COSTA_FELIPE.pdf5cf6e9d446a38e1dde45e676ab1413cfMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81956http://repositorio.ufsm.br/bitstream/1/18127/3/license.txt2f0571ecee68693bd5cd3f17c1e075dfMD53TEXTTES_PPGFARMACOLOGIA_2016_COSTA_FELIPE.pdf.txtTES_PPGFARMACOLOGIA_2016_COSTA_FELIPE.pdf.txtExtracted texttext/plain149240http://repositorio.ufsm.br/bitstream/1/18127/4/TES_PPGFARMACOLOGIA_2016_COSTA_FELIPE.pdf.txt276cfeb79cd834bf38cabb215be866b6MD54THUMBNAILTES_PPGFARMACOLOGIA_2016_COSTA_FELIPE.pdf.jpgTES_PPGFARMACOLOGIA_2016_COSTA_FELIPE.pdf.jpgIM Thumbnailimage/jpeg4693http://repositorio.ufsm.br/bitstream/1/18127/5/TES_PPGFARMACOLOGIA_2016_COSTA_FELIPE.pdf.jpg6d9abd480684b64198933fb050b8af03MD551/181272019-09-04 03:02:37.11oai:repositorio.ufsm.br:1/18127TElDRU7Dh0EgREUgRElTVFJJQlVJw4fDg08gTsODTy1FWENMVVNJVkEKCkNvbSBhIGFwcmVzZW50YcOnw6NvIGRlc3RhIGxpY2Vuw6dhLCB2b2PDqiAobyBhdXRvciAoZXMpIG91IG8gdGl0dWxhciBkb3MgZGlyZWl0b3MgZGUgYXV0b3IpIGNvbmNlZGUgw6AgVW5pdmVyc2lkYWRlCkZlZGVyYWwgZGUgU2FudGEgTWFyaWEgKFVGU00pIG8gZGlyZWl0byBuw6NvLWV4Y2x1c2l2byBkZSByZXByb2R1emlyLCAgdHJhZHV6aXIgKGNvbmZvcm1lIGRlZmluaWRvIGFiYWl4byksIGUvb3UKZGlzdHJpYnVpciBhIHN1YSB0ZXNlIG91IGRpc3NlcnRhw6fDo28gKGluY2x1aW5kbyBvIHJlc3VtbykgcG9yIHRvZG8gbyBtdW5kbyBubyBmb3JtYXRvIGltcHJlc3NvIGUgZWxldHLDtG5pY28gZQplbSBxdWFscXVlciBtZWlvLCBpbmNsdWluZG8gb3MgZm9ybWF0b3Mgw6F1ZGlvIG91IHbDrWRlby4KClZvY8OqIGNvbmNvcmRhIHF1ZSBhIFVGU00gcG9kZSwgc2VtIGFsdGVyYXIgbyBjb250ZcO6ZG8sIHRyYW5zcG9yIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbwpwYXJhIHF1YWxxdWVyIG1laW8gb3UgZm9ybWF0byBwYXJhIGZpbnMgZGUgcHJlc2VydmHDp8Ojby4KClZvY8OqIHRhbWLDqW0gY29uY29yZGEgcXVlIGEgVUZTTSBwb2RlIG1hbnRlciBtYWlzIGRlIHVtYSBjw7NwaWEgYSBzdWEgdGVzZSBvdQpkaXNzZXJ0YcOnw6NvIHBhcmEgZmlucyBkZSBzZWd1cmFuw6dhLCBiYWNrLXVwIGUgcHJlc2VydmHDp8Ojby4KClZvY8OqIGRlY2xhcmEgcXVlIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyDDqSBvcmlnaW5hbCBlIHF1ZSB2b2PDqiB0ZW0gbyBwb2RlciBkZSBjb25jZWRlciBvcyBkaXJlaXRvcyBjb250aWRvcwpuZXN0YSBsaWNlbsOnYS4gVm9jw6ogdGFtYsOpbSBkZWNsYXJhIHF1ZSBvIGRlcMOzc2l0byBkYSBzdWEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvIG7Do28sIHF1ZSBzZWphIGRlIHNldQpjb25oZWNpbWVudG8sIGluZnJpbmdlIGRpcmVpdG9zIGF1dG9yYWlzIGRlIG5pbmd1w6ltLgoKQ2FzbyBhIHN1YSB0ZXNlIG91IGRpc3NlcnRhw6fDo28gY29udGVuaGEgbWF0ZXJpYWwgcXVlIHZvY8OqIG7Do28gcG9zc3VpIGEgdGl0dWxhcmlkYWRlIGRvcyBkaXJlaXRvcyBhdXRvcmFpcywgdm9jw6oKZGVjbGFyYSBxdWUgb2J0ZXZlIGEgcGVybWlzc8OjbyBpcnJlc3RyaXRhIGRvIGRldGVudG9yIGRvcyBkaXJlaXRvcyBhdXRvcmFpcyBwYXJhIGNvbmNlZGVyIMOgIFVGU00Kb3MgZGlyZWl0b3MgYXByZXNlbnRhZG9zIG5lc3RhIGxpY2Vuw6dhLCBlIHF1ZSBlc3NlIG1hdGVyaWFsIGRlIHByb3ByaWVkYWRlIGRlIHRlcmNlaXJvcyBlc3TDoSBjbGFyYW1lbnRlCmlkZW50aWZpY2FkbyBlIHJlY29uaGVjaWRvIG5vIHRleHRvIG91IG5vIGNvbnRlw7pkbyBkYSB0ZXNlIG91IGRpc3NlcnRhw6fDo28gb3JhIGRlcG9zaXRhZGEuCgpDQVNPIEEgVEVTRSBPVSBESVNTRVJUQcOHw4NPIE9SQSBERVBPU0lUQURBIFRFTkhBIFNJRE8gUkVTVUxUQURPIERFIFVNIFBBVFJPQ8ONTklPIE9VCkFQT0lPIERFIFVNQSBBR8OKTkNJQSBERSBGT01FTlRPIE9VIE9VVFJPIE9SR0FOSVNNTyBRVUUgTsODTyBTRUpBIEEgVUZTTQosIFZPQ8OKIERFQ0xBUkEgUVVFIFJFU1BFSVRPVSBUT0RPUyBFIFFVQUlTUVVFUiBESVJFSVRPUyBERSBSRVZJU8ODTyBDT01PClRBTULDiU0gQVMgREVNQUlTIE9CUklHQcOHw5VFUyBFWElHSURBUyBQT1IgQ09OVFJBVE8gT1UgQUNPUkRPLgoKQSBVRlNNIHNlIGNvbXByb21ldGUgYSBpZGVudGlmaWNhciBjbGFyYW1lbnRlIG8gc2V1IG5vbWUgKHMpIG91IG8ocykgbm9tZShzKSBkbyhzKQpkZXRlbnRvcihlcykgZG9zIGRpcmVpdG9zIGF1dG9yYWlzIGRhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbywgZSBuw6NvIGZhcsOhIHF1YWxxdWVyIGFsdGVyYcOnw6NvLCBhbMOpbSBkYXF1ZWxhcwpjb25jZWRpZGFzIHBvciBlc3RhIGxpY2Vuw6dhLgoKBiblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2019-09-04T06:02:37Biblioteca Digital de Teses e Dissertações do UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Efeito do desbalanço genético e farmacológico do metabolismo oxidativo em indicadores de fertilidade masculina |
dc.title.alternative.eng.fl_str_mv |
Effect of genetic and pharmacological disbursement of oxidative metabolism in male fertility indicator |
title |
Efeito do desbalanço genético e farmacológico do metabolismo oxidativo em indicadores de fertilidade masculina |
spellingShingle |
Efeito do desbalanço genético e farmacológico do metabolismo oxidativo em indicadores de fertilidade masculina Costa, Felipe Infertilidade Polimorfismo Val16Ala SOD2 Qualidade espermática Estresse oxidativo Infertility Val16Ala-SOD2 polymorphism Sperm quality Oxidative stress CNPQ::CIENCIAS DA SAUDE::FARMACIA |
title_short |
Efeito do desbalanço genético e farmacológico do metabolismo oxidativo em indicadores de fertilidade masculina |
title_full |
Efeito do desbalanço genético e farmacológico do metabolismo oxidativo em indicadores de fertilidade masculina |
title_fullStr |
Efeito do desbalanço genético e farmacológico do metabolismo oxidativo em indicadores de fertilidade masculina |
title_full_unstemmed |
Efeito do desbalanço genético e farmacológico do metabolismo oxidativo em indicadores de fertilidade masculina |
title_sort |
Efeito do desbalanço genético e farmacológico do metabolismo oxidativo em indicadores de fertilidade masculina |
author |
Costa, Felipe |
author_facet |
Costa, Felipe |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Cruz, Ivana Beatrice Mânica da |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/3426369324110716 |
dc.contributor.referee1.fl_str_mv |
Fachinetto, Roselei |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/7203076675431306 |
dc.contributor.referee2.fl_str_mv |
Bresciani, Guilherme Bergamaschi |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/1247251997170368 |
dc.contributor.referee3.fl_str_mv |
Comim, Fabio Vasconcellos |
dc.contributor.referee3Lattes.fl_str_mv |
http://lattes.cnpq.br/5119233991388822 |
dc.contributor.referee4.fl_str_mv |
Sagrillo, Michele Rorato |
dc.contributor.referee4Lattes.fl_str_mv |
http://lattes.cnpq.br/2566285176244747 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/7742630278309727 |
dc.contributor.author.fl_str_mv |
Costa, Felipe |
contributor_str_mv |
Cruz, Ivana Beatrice Mânica da Fachinetto, Roselei Bresciani, Guilherme Bergamaschi Comim, Fabio Vasconcellos Sagrillo, Michele Rorato |
dc.subject.por.fl_str_mv |
Infertilidade Polimorfismo Val16Ala SOD2 Qualidade espermática Estresse oxidativo |
topic |
Infertilidade Polimorfismo Val16Ala SOD2 Qualidade espermática Estresse oxidativo Infertility Val16Ala-SOD2 polymorphism Sperm quality Oxidative stress CNPQ::CIENCIAS DA SAUDE::FARMACIA |
dc.subject.eng.fl_str_mv |
Infertility Val16Ala-SOD2 polymorphism Sperm quality Oxidative stress |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS DA SAUDE::FARMACIA |
description |
Previous studies indicate that changes in oxidative metabolism may play a role in reproductive success. Manganese-dependent superoxide dismutase enzyme (SOD2), a key in the oxidative balance, presents a genetic variation Val16Ala-SOD2 that leads to a change in its efficiency causing an imbalance between the superoxide (S) and hydrogen peroxide (HP) levels of Cells. Studies have suggested that both homozygous genotypes (AA-VV) cause an S-HP imbalance associated with the risk of various chronic diseases, and interfere with female reproductive success. Thus, the objective of this work was to investigate the effect of the genetic imbalance caused by the polymorphism Val16Ala-SOD2 and pharmacological induced by the treatment with paraquat in oxidative metabolism and in male fertility indicators. For the in vitro analysis of the impact of the S-HP imbalance caused by the pharmacological action of paraquat, a method of quantification of the levels of cfDNA in the seminal plasma was conducted by fluorimetry using the DNA Picogreen. The second protocol investigated the association between the Val16Ala- SOD2 polymorphism with changes in the male fertility parameters determined by the WHO, by evaluating the association of this polymorphism with high levels of cfDNA and markers of oxidative stress in seminal plasma. Semen samples were obtained from healthy volunteers or from surplus samples used to perform sperm in laboratory clinical analyzes, the samples were processed and analyzed in the Biogenomic Laboratory, through spectrophotometric and molecular tests. The results showed a significant association between elevated levels of cfDNA and altered fertility parameters in semen, such as: low viability, changes in motility and sperm morphology. In vitro complementary analysis confirmed that high levels of S causes oxidative stress and decrease sperm viability. These results corroborated the hypothesis that cfDNA levels could be markers of general sperm quality and oxidative stress. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016-12-07 |
dc.date.accessioned.fl_str_mv |
2019-09-03T19:15:06Z |
dc.date.available.fl_str_mv |
2019-09-03T19:15:06Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/18127 |
url |
http://repositorio.ufsm.br/handle/1/18127 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.cnpq.fl_str_mv |
400300000005 |
dc.relation.confidence.fl_str_mv |
600 |
dc.relation.authority.fl_str_mv |
0b5fcfe0-b017-4163-80e2-17c110fbcf01 e2208229-12a5-4c65-b374-cbc9b69d36e7 d4a64262-0e7e-45c5-910e-0b0aee907c10 03f335df-7f03-44d1-8866-576fb27afbe0 982f3d46-745a-4f4c-83bd-1bbe76793e5b e37af8b8-3d08-474c-b996-ee17aaf6b3a8 |
dc.rights.driver.fl_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências da Saúde |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Farmacologia |
dc.publisher.initials.fl_str_mv |
UFSM |
dc.publisher.country.fl_str_mv |
Brasil |
dc.publisher.department.fl_str_mv |
Farmacologia |
publisher.none.fl_str_mv |
Universidade Federal de Santa Maria Centro de Ciências da Saúde |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações do UFSM instname:Universidade Federal de Santa Maria (UFSM) instacron:UFSM |
instname_str |
Universidade Federal de Santa Maria (UFSM) |
instacron_str |
UFSM |
institution |
UFSM |
reponame_str |
Biblioteca Digital de Teses e Dissertações do UFSM |
collection |
Biblioteca Digital de Teses e Dissertações do UFSM |
bitstream.url.fl_str_mv |
http://repositorio.ufsm.br/bitstream/1/18127/2/license_rdf http://repositorio.ufsm.br/bitstream/1/18127/1/TES_PPGFARMACOLOGIA_2016_COSTA_FELIPE.pdf http://repositorio.ufsm.br/bitstream/1/18127/3/license.txt http://repositorio.ufsm.br/bitstream/1/18127/4/TES_PPGFARMACOLOGIA_2016_COSTA_FELIPE.pdf.txt http://repositorio.ufsm.br/bitstream/1/18127/5/TES_PPGFARMACOLOGIA_2016_COSTA_FELIPE.pdf.jpg |
bitstream.checksum.fl_str_mv |
4460e5956bc1d1639be9ae6146a50347 5cf6e9d446a38e1dde45e676ab1413cf 2f0571ecee68693bd5cd3f17c1e075df 276cfeb79cd834bf38cabb215be866b6 6d9abd480684b64198933fb050b8af03 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 MD5 |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações do UFSM - Universidade Federal de Santa Maria (UFSM) |
repository.mail.fl_str_mv |
atendimento.sib@ufsm.br||tedebc@gmail.com |
_version_ |
1793240126383456256 |