Extração assistida por ultrassom de β-glucanos de Lentinula edodes utilizando solvente eutético e água
Ano de defesa: | 2022 |
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Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Santa Maria
Centro de Tecnologia |
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Engenharia Química
|
Departamento: |
Engenharia Química
|
País: |
Brasil
|
Palavras-chave em Português: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.ufsm.br/handle/1/24348 |
Resumo: | Shiitake is a mushroom rich in β-glucan type polysaccharides, long-chain molecules that have antioxidant properties that are beneficial to human health, in addition to having isolated physical properties of interest to the food industry. In this way, the main objective of this study was to obtain shiitake β-glucans through ultrasound-assisted extraction. Preliminary tests were carried out to determine the temperature and the extraction time. Subsequently, a central composite rotational design (CCRD) varying the pulsation cycles and amplitude was performed where the yield and antioxidant activity of the extracts were obtained. Extractions were carried out using two solvents, water and a deep eutectic solvent (choline chloride: glycerol). The best yields using eutectic solvent were 25.11 and 23.74% in 88.36% amplitude and 0.93 pulse cycle and 100% amplitude and 0.75 pulse cycle, respectively, while for water it was of 23.47 and 22.75% with 100% amplitude and 0.75 pulse cycle and 88.36% amplitude and 0.93 pulse cycle, respectively. Analyzes of total sugars, proteins, and Fourier transform infrared spectroscopy (FT-IR) were performed on the ethanol precipitated extracts, obtaining higher concentrations of sugars under the same conditions as the best yields, while proteins were better extracted at intermediate amplitudes using water and higher amplitudes with eutectic solvent. In addition, bands were found in the FT-IR similar to other studies that analyzed β-glucans. Thus, this study evaluated a eutectic solvent not used so far in the ultrasonic extraction of antioxidant compounds, demonstrating an alternative to obtain compounds with properties of interest to the food or pharmaceutical industry from shiitake outdated for human consumption. |
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2022-05-13T13:30:19Z2022-05-13T13:30:19Z2022-03-30http://repositorio.ufsm.br/handle/1/24348Shiitake is a mushroom rich in β-glucan type polysaccharides, long-chain molecules that have antioxidant properties that are beneficial to human health, in addition to having isolated physical properties of interest to the food industry. In this way, the main objective of this study was to obtain shiitake β-glucans through ultrasound-assisted extraction. Preliminary tests were carried out to determine the temperature and the extraction time. Subsequently, a central composite rotational design (CCRD) varying the pulsation cycles and amplitude was performed where the yield and antioxidant activity of the extracts were obtained. Extractions were carried out using two solvents, water and a deep eutectic solvent (choline chloride: glycerol). The best yields using eutectic solvent were 25.11 and 23.74% in 88.36% amplitude and 0.93 pulse cycle and 100% amplitude and 0.75 pulse cycle, respectively, while for water it was of 23.47 and 22.75% with 100% amplitude and 0.75 pulse cycle and 88.36% amplitude and 0.93 pulse cycle, respectively. Analyzes of total sugars, proteins, and Fourier transform infrared spectroscopy (FT-IR) were performed on the ethanol precipitated extracts, obtaining higher concentrations of sugars under the same conditions as the best yields, while proteins were better extracted at intermediate amplitudes using water and higher amplitudes with eutectic solvent. In addition, bands were found in the FT-IR similar to other studies that analyzed β-glucans. Thus, this study evaluated a eutectic solvent not used so far in the ultrasonic extraction of antioxidant compounds, demonstrating an alternative to obtain compounds with properties of interest to the food or pharmaceutical industry from shiitake outdated for human consumption.O shiitake é um cogumelo rico em polissacarídeos do tipo β-glucanos, moléculas de cadeia longa, que apresentam propriedades antioxidantes e benéficas para a saúde humana, além de apresentarem propriedades físico-químicas de interesse para a indústria alimentícia. Dessa forma, o principal objetivo deste estudo foi a obtenção de β-glucanos do shiitake através de extração assistida por ultrassom. Ensaios preliminares foram realizados para determinar a temperatura e o tempo de extração. Posteriormente, um delineamento composto central rotacional (DCCR) variando os ciclos de pulsação e a amplitude foi realizado onde o rendimento e a atividade antioxidante dos extratos foram avaliados. As extrações foram realizadas utilizando dois solventes, a água e um solvente eutético (cloreto de colina: glicerol). Os melhores rendimentos utilizando solvente eutético foram de 25,11 e 23,74% sob 88,36% de amplitude e 0,93 ciclo de pulsação e 100% de amplitude e 0,75 ciclo de pulsação, respectivamente. Enquanto que para água o rendimento foi de 23,47 e 22,75% com 100% de amplitude e 0,75 ciclo de pulsação e 88,36% de amplitude e 0,93 ciclo de pulsação, respectivamente. Análises de açúcares totais, proteínas e espectroscopia de infravermelho por transformada de Fourier (FT-IR) foram realizadas nos extratos precipitados em etanol, obtendo maiores concentrações de açúcares nas mesmas condições que os melhores rendimentos, enquanto que as proteínas foram melhor extraídas em amplitudes intermediárias usando água e maiores amplitudes com solvente eutético. Além disso, foram encontradas bandas no FT-IR semelhantes a outros estudos que analisaram β-glucanos. Assim, este estudo avaliou um solvente eutético até o momento não utilizado na extração por ultrassom de compostos antioxidantes, demonstrando uma alternativa para obtenção de compostos com propriedades de interesse para a indústria de alimentos ou farmacêutica a partir de shiitake fora do prazo para consumo humano.porUniversidade Federal de Santa MariaCentro de TecnologiaPrograma de Pós-Graduação em Engenharia QuímicaUFSMBrasilEngenharia QuímicaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessShiitakeUltrassomSolvente eutéticoPolissacarídeosCNPQ::ENGENHARIAS::ENGENHARIA QUIMICAExtração assistida por ultrassom de β-glucanos de Lentinula edodes utilizando solvente eutético e águaUltrasound-assisted extraction of β-glucans from Lentinula edodes using deep eutectic solvent and waterinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisKuhn, Raquel Cristinehttp://lattes.cnpq.br/1479407050883691Abaide, Ederson RossiZiotti, Ana Paula Manerahttp://lattes.cnpq.br/5130714625938208Vezaro, Francisco Dalcin3006000000066006006006006003528ba34-a916-462d-8700-48f0999fc4623038b645-5d32-4415-9d69-735ed28d0187d4801dd4-72a8-4bf5-9c83-d7ac2d170357a6634b2e-6bda-477d-9711-17e62a69c408reponame:Biblioteca Digital de Teses e Dissertações do UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMCC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8805http://repositorio.ufsm.br/bitstream/1/24348/2/license_rdf4460e5956bc1d1639be9ae6146a50347MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-816http://repositorio.ufsm.br/bitstream/1/24348/3/license.txtf8fcb28efb1c8cf0dc096bec902bf4c4MD53ORIGINALDIS_PPGEQ_2022_VEZARO_FRANCISCO.pdfDIS_PPGEQ_2022_VEZARO_FRANCISCO.pdfDissertaçãoapplication/pdf2184448http://repositorio.ufsm.br/bitstream/1/24348/1/DIS_PPGEQ_2022_VEZARO_FRANCISCO.pdf472731e66bd65c66bd0da6b5e97efc93MD511/243482022-05-13 10:30:19.947oai:repositorio.ufsm.br:1/24348Q3JlYXRpdmUgQ29tbW9ucw==Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-05-13T13:30:19Biblioteca Digital de Teses e Dissertações do UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Extração assistida por ultrassom de β-glucanos de Lentinula edodes utilizando solvente eutético e água |
dc.title.alternative.eng.fl_str_mv |
Ultrasound-assisted extraction of β-glucans from Lentinula edodes using deep eutectic solvent and water |
title |
Extração assistida por ultrassom de β-glucanos de Lentinula edodes utilizando solvente eutético e água |
spellingShingle |
Extração assistida por ultrassom de β-glucanos de Lentinula edodes utilizando solvente eutético e água Vezaro, Francisco Dalcin Shiitake Ultrassom Solvente eutético Polissacarídeos CNPQ::ENGENHARIAS::ENGENHARIA QUIMICA |
title_short |
Extração assistida por ultrassom de β-glucanos de Lentinula edodes utilizando solvente eutético e água |
title_full |
Extração assistida por ultrassom de β-glucanos de Lentinula edodes utilizando solvente eutético e água |
title_fullStr |
Extração assistida por ultrassom de β-glucanos de Lentinula edodes utilizando solvente eutético e água |
title_full_unstemmed |
Extração assistida por ultrassom de β-glucanos de Lentinula edodes utilizando solvente eutético e água |
title_sort |
Extração assistida por ultrassom de β-glucanos de Lentinula edodes utilizando solvente eutético e água |
author |
Vezaro, Francisco Dalcin |
author_facet |
Vezaro, Francisco Dalcin |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Kuhn, Raquel Cristine |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/1479407050883691 |
dc.contributor.referee1.fl_str_mv |
Abaide, Ederson Rossi |
dc.contributor.referee2.fl_str_mv |
Ziotti, Ana Paula Manera |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/5130714625938208 |
dc.contributor.author.fl_str_mv |
Vezaro, Francisco Dalcin |
contributor_str_mv |
Kuhn, Raquel Cristine Abaide, Ederson Rossi Ziotti, Ana Paula Manera |
dc.subject.por.fl_str_mv |
Shiitake Ultrassom Solvente eutético Polissacarídeos |
topic |
Shiitake Ultrassom Solvente eutético Polissacarídeos CNPQ::ENGENHARIAS::ENGENHARIA QUIMICA |
dc.subject.cnpq.fl_str_mv |
CNPQ::ENGENHARIAS::ENGENHARIA QUIMICA |
description |
Shiitake is a mushroom rich in β-glucan type polysaccharides, long-chain molecules that have antioxidant properties that are beneficial to human health, in addition to having isolated physical properties of interest to the food industry. In this way, the main objective of this study was to obtain shiitake β-glucans through ultrasound-assisted extraction. Preliminary tests were carried out to determine the temperature and the extraction time. Subsequently, a central composite rotational design (CCRD) varying the pulsation cycles and amplitude was performed where the yield and antioxidant activity of the extracts were obtained. Extractions were carried out using two solvents, water and a deep eutectic solvent (choline chloride: glycerol). The best yields using eutectic solvent were 25.11 and 23.74% in 88.36% amplitude and 0.93 pulse cycle and 100% amplitude and 0.75 pulse cycle, respectively, while for water it was of 23.47 and 22.75% with 100% amplitude and 0.75 pulse cycle and 88.36% amplitude and 0.93 pulse cycle, respectively. Analyzes of total sugars, proteins, and Fourier transform infrared spectroscopy (FT-IR) were performed on the ethanol precipitated extracts, obtaining higher concentrations of sugars under the same conditions as the best yields, while proteins were better extracted at intermediate amplitudes using water and higher amplitudes with eutectic solvent. In addition, bands were found in the FT-IR similar to other studies that analyzed β-glucans. Thus, this study evaluated a eutectic solvent not used so far in the ultrasonic extraction of antioxidant compounds, demonstrating an alternative to obtain compounds with properties of interest to the food or pharmaceutical industry from shiitake outdated for human consumption. |
publishDate |
2022 |
dc.date.accessioned.fl_str_mv |
2022-05-13T13:30:19Z |
dc.date.available.fl_str_mv |
2022-05-13T13:30:19Z |
dc.date.issued.fl_str_mv |
2022-03-30 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
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http://repositorio.ufsm.br/handle/1/24348 |
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http://repositorio.ufsm.br/handle/1/24348 |
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por |
language |
por |
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300600000006 |
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600 600 600 600 600 |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess |
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Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ |
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openAccess |
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Universidade Federal de Santa Maria Centro de Tecnologia |
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Programa de Pós-Graduação em Engenharia Química |
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UFSM |
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Brasil |
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Engenharia Química |
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Universidade Federal de Santa Maria Centro de Tecnologia |
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