Atividade in vitro dos óleos essenciais de condimentos frente a Candida glabrata sensíveis e resistenes ao fluconazol

Detalhes bibliográficos
Ano de defesa: 2012
Autor(a) principal: Soares, Isaura Helena lattes
Orientador(a): Alves, Sydney Hartz lattes
Banca de defesa: Moreira, Cleci Menezes lattes, Fries, Leadir Lucy Martins lattes
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal de Santa Maria
Programa de Pós-Graduação: Programa de Pós-Graduação em Ciências Farmacêuticas
Departamento: Farmacologia
País: BR
Palavras-chave em Português:
Candida glabrata
Óleos essenciais
Atividade antifúngica
Palavras-chave em Inglês:
Essential oil
Antifungal activity
Área do conhecimento CNPq:
CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA
Link de acesso: http://repositorio.ufsm.br/handle/1/5978
Resumo: In the present study, in vitro antifungal activity of essential oils obtained from Origanum vulgare (oregano), Cinnamomum zeylanicum (cinnamon), Lippia graveolens (Mexican oregano), Thymus vulgaris (thyme), Salvia officinalis (sage), Rosmarinus officinalis (rosemary), Ocimum basilicum (basil) e Zingiber officinale (ginger) was assessed against Candida glabrata isolates. A group of 36 fluconazole-susceptible C. glabrata isolates were used and another group, derived from the first, obtained after in vitro induction of the fluconazole-resistance, totalizing 72 isolates tested. The methodology used was broth microdilution, cancentrations of 50 μg/mL, 100 μg/mL, 200 μg/mL, 400 μg/mL, 800 μg/mL, 1600 μg/mL and 3200 μg/mL were used, through which Minimal Inhibitory Concentration (MIC) and Minimal Fungicidal Concentration (MFC) were determined. Thyme, sage, rosemary, basil and ginger essential oils showed no antifungal activity on the tested concentrations. Antimicrobial activity lower than or equal to 3200 μg/mL was observed for oregano, Mexican oregano and cinnamon essential oils, being oregano essential oil the most potent. Oregano essential oil has shown the best antifungal activity against fluconazole-susceptible C. glabrata group. Likely, Mexican oregano essential oil has shown the best antifungal activity against fluconazole-susceptible C. glabrata group. And cinnamon essential oil has shown the best antifungal activity against fluconazole-resistant isolates of C. glabrata.
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spelling 2015-02-122015-02-122012-08-17SOARES, Isaura Helena. In vitro ACTIVITY OF ESSENTIAL OILS EXTRACTED FROM CONDIMENTS AGAINST FLUCONAZOLE RESISTANT AND SENSIBLE Candida glabrata. 2012. 57 f. Dissertação (Mestrado em Farmacologia) - Universidade Federal de Santa Maria, Santa Maria, 2012.http://repositorio.ufsm.br/handle/1/5978In the present study, in vitro antifungal activity of essential oils obtained from Origanum vulgare (oregano), Cinnamomum zeylanicum (cinnamon), Lippia graveolens (Mexican oregano), Thymus vulgaris (thyme), Salvia officinalis (sage), Rosmarinus officinalis (rosemary), Ocimum basilicum (basil) e Zingiber officinale (ginger) was assessed against Candida glabrata isolates. A group of 36 fluconazole-susceptible C. glabrata isolates were used and another group, derived from the first, obtained after in vitro induction of the fluconazole-resistance, totalizing 72 isolates tested. The methodology used was broth microdilution, cancentrations of 50 μg/mL, 100 μg/mL, 200 μg/mL, 400 μg/mL, 800 μg/mL, 1600 μg/mL and 3200 μg/mL were used, through which Minimal Inhibitory Concentration (MIC) and Minimal Fungicidal Concentration (MFC) were determined. Thyme, sage, rosemary, basil and ginger essential oils showed no antifungal activity on the tested concentrations. Antimicrobial activity lower than or equal to 3200 μg/mL was observed for oregano, Mexican oregano and cinnamon essential oils, being oregano essential oil the most potent. Oregano essential oil has shown the best antifungal activity against fluconazole-susceptible C. glabrata group. Likely, Mexican oregano essential oil has shown the best antifungal activity against fluconazole-susceptible C. glabrata group. And cinnamon essential oil has shown the best antifungal activity against fluconazole-resistant isolates of C. glabrata.No presente estudo, avaliou-se a atividade antimicrobiana in vitro dos óleos essenciais obtidos de Origanum vulgare (orégano), Cinnamomum zeylanicum (canela), Lippia graveolens (orégano mexicano), Thymus vulgaris (tomilho), Salvia officinalis (sálvia), Rosmarinus officinalis (alecrim), Ocimum basilicum (manjericão) e Zingiber officinale (gengibre) frente aos isolados de Candida glabrata. Foi utilizado um grupo de 36 isolados de C. glabrata sensíveis ao fluconazol e outro grupo, derivado do primeiro, obtido após indução a resistência ao fluconazol in vitro, totalizando 72 isolados testados. Empregou-se a metodologia de microdiluição em caldo, utilizando-se concentrações de 50 μg/mL, 100 μg/mL, 200 μg/mL, 400 μg/mL, 800 μg/mL, 1600 μg/mL e 3200 μg/mL, através do qual se determinou Concentração Inibitória Mínima (CIM) e Concentração Fungicida Mínima (CFM). Os óleos essenciais de tomilho, sálvia, alecrim, manjericão e gengibre não evidenciaram atividade antifúngica nas concentrações testadas. Atividade antimicrobiana inferior ou igual à máxima testada (3200 μg/mL) foi observada para os óleos essenciais de orégano, orégano mexicano e canela. O óleo essencial de orégano apresentou a atividade antimicrobiana mais potente. Quando analisada a diferença entre os dois grupos de micro-organismos, C. glabrata sensível ao fluconazol mostrou-se mais suscetível ao óleo essencial de orégano e orégano mexicano, e C. glabrata resistente ao fluconazol mostrou-se mais suscetível ao óleo essencial de canela.application/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em Ciências FarmacêuticasUFSMBRFarmacologiaCandida glabrataÓleos essenciaisAtividade antifúngicaEssential oilAntifungal activityCNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIAAtividade in vitro dos óleos essenciais de condimentos frente a Candida glabrata sensíveis e resistenes ao fluconazolIn vitro activity of essential oils extracted from condiments against fluconazole resistant and sensible Candida glabratainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisAlves, Sydney Hartzhttp://lattes.cnpq.br/0330782478769631Moreira, Cleci Menezeshttp://lattes.cnpq.br/5805841991374556Fries, Leadir Lucy Martinshttp://lattes.cnpq.br/5099404254254292http://lattes.cnpq.br/7103468760581101Soares, Isaura Helena201000000000400500300500500bfe6016c-9c1e-42ca-ab1d-db5453ed090c163c207f-2353-4f6f-b850-646cd6af7cbc42b89fc7-1153-4da6-b5b3-8f2f688fad00259ee132-f44e-4af7-a95c-683d87867435info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações do UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALSOARES, ISAURA HELENA.pdfapplication/pdf372598http://repositorio.ufsm.br/bitstream/1/5978/1/SOARES%2c%20ISAURA%20HELENA.pdfd1f9ee04055f32bc2a15a874f279375dMD51TEXTSOARES, ISAURA HELENA.pdf.txtSOARES, ISAURA HELENA.pdf.txtExtracted texttext/plain116856http://repositorio.ufsm.br/bitstream/1/5978/2/SOARES%2c%20ISAURA%20HELENA.pdf.txt095024c3cd09e3a53f8235d8fa92e6f7MD52THUMBNAILSOARES, ISAURA HELENA.pdf.jpgSOARES, ISAURA HELENA.pdf.jpgIM Thumbnailimage/jpeg5035http://repositorio.ufsm.br/bitstream/1/5978/3/SOARES%2c%20ISAURA%20HELENA.pdf.jpg52aa54dbb39851ba033e9ba98e16b01aMD531/59782021-09-24 15:11:53.124oai:repositorio.ufsm.br:1/5978Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2021-09-24T18:11:53Biblioteca Digital de Teses e Dissertações do UFSM - Universidade Federal de Santa Maria (UFSM)false
dc.title.por.fl_str_mv Atividade in vitro dos óleos essenciais de condimentos frente a Candida glabrata sensíveis e resistenes ao fluconazol
dc.title.alternative.eng.fl_str_mv In vitro activity of essential oils extracted from condiments against fluconazole resistant and sensible Candida glabrata
title Atividade in vitro dos óleos essenciais de condimentos frente a Candida glabrata sensíveis e resistenes ao fluconazol
spellingShingle Atividade in vitro dos óleos essenciais de condimentos frente a Candida glabrata sensíveis e resistenes ao fluconazol
Soares, Isaura Helena
Candida glabrata
Óleos essenciais
Atividade antifúngica
Essential oil
Antifungal activity
CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA
title_short Atividade in vitro dos óleos essenciais de condimentos frente a Candida glabrata sensíveis e resistenes ao fluconazol
title_full Atividade in vitro dos óleos essenciais de condimentos frente a Candida glabrata sensíveis e resistenes ao fluconazol
title_fullStr Atividade in vitro dos óleos essenciais de condimentos frente a Candida glabrata sensíveis e resistenes ao fluconazol
title_full_unstemmed Atividade in vitro dos óleos essenciais de condimentos frente a Candida glabrata sensíveis e resistenes ao fluconazol
title_sort Atividade in vitro dos óleos essenciais de condimentos frente a Candida glabrata sensíveis e resistenes ao fluconazol
author Soares, Isaura Helena
author_facet Soares, Isaura Helena
author_role author
dc.contributor.advisor1.fl_str_mv Alves, Sydney Hartz
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/0330782478769631
dc.contributor.referee1.fl_str_mv Moreira, Cleci Menezes
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/5805841991374556
dc.contributor.referee2.fl_str_mv Fries, Leadir Lucy Martins
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/5099404254254292
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/7103468760581101
dc.contributor.author.fl_str_mv Soares, Isaura Helena
contributor_str_mv Alves, Sydney Hartz
Moreira, Cleci Menezes
Fries, Leadir Lucy Martins
dc.subject.por.fl_str_mv Candida glabrata
Óleos essenciais
Atividade antifúngica
topic Candida glabrata
Óleos essenciais
Atividade antifúngica
Essential oil
Antifungal activity
CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA
dc.subject.eng.fl_str_mv Essential oil
Antifungal activity
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA
description In the present study, in vitro antifungal activity of essential oils obtained from Origanum vulgare (oregano), Cinnamomum zeylanicum (cinnamon), Lippia graveolens (Mexican oregano), Thymus vulgaris (thyme), Salvia officinalis (sage), Rosmarinus officinalis (rosemary), Ocimum basilicum (basil) e Zingiber officinale (ginger) was assessed against Candida glabrata isolates. A group of 36 fluconazole-susceptible C. glabrata isolates were used and another group, derived from the first, obtained after in vitro induction of the fluconazole-resistance, totalizing 72 isolates tested. The methodology used was broth microdilution, cancentrations of 50 μg/mL, 100 μg/mL, 200 μg/mL, 400 μg/mL, 800 μg/mL, 1600 μg/mL and 3200 μg/mL were used, through which Minimal Inhibitory Concentration (MIC) and Minimal Fungicidal Concentration (MFC) were determined. Thyme, sage, rosemary, basil and ginger essential oils showed no antifungal activity on the tested concentrations. Antimicrobial activity lower than or equal to 3200 μg/mL was observed for oregano, Mexican oregano and cinnamon essential oils, being oregano essential oil the most potent. Oregano essential oil has shown the best antifungal activity against fluconazole-susceptible C. glabrata group. Likely, Mexican oregano essential oil has shown the best antifungal activity against fluconazole-susceptible C. glabrata group. And cinnamon essential oil has shown the best antifungal activity against fluconazole-resistant isolates of C. glabrata.
publishDate 2012
dc.date.issued.fl_str_mv 2012-08-17
dc.date.accessioned.fl_str_mv 2015-02-12
dc.date.available.fl_str_mv 2015-02-12
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dc.identifier.citation.fl_str_mv SOARES, Isaura Helena. In vitro ACTIVITY OF ESSENTIAL OILS EXTRACTED FROM CONDIMENTS AGAINST FLUCONAZOLE RESISTANT AND SENSIBLE Candida glabrata. 2012. 57 f. Dissertação (Mestrado em Farmacologia) - Universidade Federal de Santa Maria, Santa Maria, 2012.
dc.identifier.uri.fl_str_mv http://repositorio.ufsm.br/handle/1/5978
identifier_str_mv SOARES, Isaura Helena. In vitro ACTIVITY OF ESSENTIAL OILS EXTRACTED FROM CONDIMENTS AGAINST FLUCONAZOLE RESISTANT AND SENSIBLE Candida glabrata. 2012. 57 f. Dissertação (Mestrado em Farmacologia) - Universidade Federal de Santa Maria, Santa Maria, 2012.
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dc.publisher.country.fl_str_mv BR
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