Hemograma e mielograma de cães infectados experimentalmente com Rangelia vitalii
Ano de defesa: | 2013 |
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Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Santa Maria
|
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Medicina Veterinária
|
Departamento: |
Medicina Veterinária
|
País: |
BR
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.ufsm.br/handle/1/10160 |
Resumo: | Rangelia vitalii is a member of the protozoan phylum Apicomplexa, class Sporozoasida, order Piroplasmorida, that causes a disease called rangeliosis in dogs, popularly known as " bleeding plague" or "nambyuvú". The transmission of this parasite occurs through tick and affects dogs in rural and periurban areas especially in hotter times of the year, which favors an increase in ticks population. To date this disease has been described only in Brazil. Rangeliosis is characterized by anemia, jaundice, fever, splenomegaly, generalized lymph node enlargement, hemorrhage along the gastrointestinal tract, and persistent bleeding through the tips of the pinnae, external surface of the ears, nose, and oral cavity. The aim of this study was to evaluate hematologic changes and consequent bone marrow response during the acute phase in dogs experimentally infected with Rangelia vitalii. 12 dogs were, divided into two groups: group A consisted of five healthy animals, and group B consisted of seven dogs experimentally infected with R. vitalii. After inoculation, the animals were monitored for parasitemia by blood smears from the tips of the ear. In group B the parasite was found within erythrocytes, neutrophils and monocytes five days post-inoculation (PI). Blood samples with EDTA for complete blood count (CBC) were performed on days 0, 10, 20 and 30 PI and on days 10 to 20 PI the reticulocyte count. After blood collection, the dogs were anesthetized bone marrow aspiration for the myelogram. This study demonstrated a reduction (P <0.01) in erythrocyte count, hematocrit and hemoglobin in the infected group compared to control. On day 10 PI the infected animals showed a normocytic normochromic anemia, however, on day 20 PI was observed macrocytic hypochromic anemia and increased (P <0.05) in the red cell distribution width (RDW) in the infected group compared to control. A decreased (P <0.05) in total leukocyte count as a result of a decrease in neutrophil and eosinophil count was observed in the infected animals (P <0.05). In the infected group there was also an increase in lymphocyte and monocyte count in comparasion to control (P <0.05). Increased (P <0.01) in the reticulocyte count was observed in the infected group compared to the control group. The myelogram showed a decrease (P <0.05) in myeloid:erythroid ratio as a result of increased erythroid precursors (rubriblast, prorubricyte, rubricyte and metarubricyte) and a decrease in the myeloid lineage (myelocyte, band and segmented). Other cells such as lymphocytes, plasma cells and macrophages were more frequent in bone marrow on day 20 PI in the infected group compared to the control group. Our results, we conclude that rangeliosis can lead to a regenerative, immunomediated, hemolytic anemia, together with leukopenia due to neutropenia and eosinopenia, as well as lymphocytosis and monocytosis. These findings were observed in the acute phase experimental of infection and are similar to those found in cases of natural infection at different stages of the disease. |
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2014-01-222014-01-222013-02-27FRANÇA, Raqueli Teresinha. Hemogram and myelogram dogs infected with experimentally Rangelia vitalii. 2013. 36 f. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2013.http://repositorio.ufsm.br/handle/1/10160Rangelia vitalii is a member of the protozoan phylum Apicomplexa, class Sporozoasida, order Piroplasmorida, that causes a disease called rangeliosis in dogs, popularly known as " bleeding plague" or "nambyuvú". The transmission of this parasite occurs through tick and affects dogs in rural and periurban areas especially in hotter times of the year, which favors an increase in ticks population. To date this disease has been described only in Brazil. Rangeliosis is characterized by anemia, jaundice, fever, splenomegaly, generalized lymph node enlargement, hemorrhage along the gastrointestinal tract, and persistent bleeding through the tips of the pinnae, external surface of the ears, nose, and oral cavity. The aim of this study was to evaluate hematologic changes and consequent bone marrow response during the acute phase in dogs experimentally infected with Rangelia vitalii. 12 dogs were, divided into two groups: group A consisted of five healthy animals, and group B consisted of seven dogs experimentally infected with R. vitalii. After inoculation, the animals were monitored for parasitemia by blood smears from the tips of the ear. In group B the parasite was found within erythrocytes, neutrophils and monocytes five days post-inoculation (PI). Blood samples with EDTA for complete blood count (CBC) were performed on days 0, 10, 20 and 30 PI and on days 10 to 20 PI the reticulocyte count. After blood collection, the dogs were anesthetized bone marrow aspiration for the myelogram. This study demonstrated a reduction (P <0.01) in erythrocyte count, hematocrit and hemoglobin in the infected group compared to control. On day 10 PI the infected animals showed a normocytic normochromic anemia, however, on day 20 PI was observed macrocytic hypochromic anemia and increased (P <0.05) in the red cell distribution width (RDW) in the infected group compared to control. A decreased (P <0.05) in total leukocyte count as a result of a decrease in neutrophil and eosinophil count was observed in the infected animals (P <0.05). In the infected group there was also an increase in lymphocyte and monocyte count in comparasion to control (P <0.05). Increased (P <0.01) in the reticulocyte count was observed in the infected group compared to the control group. The myelogram showed a decrease (P <0.05) in myeloid:erythroid ratio as a result of increased erythroid precursors (rubriblast, prorubricyte, rubricyte and metarubricyte) and a decrease in the myeloid lineage (myelocyte, band and segmented). Other cells such as lymphocytes, plasma cells and macrophages were more frequent in bone marrow on day 20 PI in the infected group compared to the control group. Our results, we conclude that rangeliosis can lead to a regenerative, immunomediated, hemolytic anemia, together with leukopenia due to neutropenia and eosinopenia, as well as lymphocytosis and monocytosis. These findings were observed in the acute phase experimental of infection and are similar to those found in cases of natural infection at different stages of the disease.Rangelia vitalii é um protozoário do filo Apicomplexa, classe Sporozoasida, ordem Piroplasmorida, que provoca, em caninos, uma doença denominada de rangeliose conhecida popularmente como "peste do sangue" ou "nambyuvú". A transmissão deste parasito ocorre através do carrapato e afeta cães das zonas rurais e periurbanas principalmente nas épocas mais quentes do ano, que favorecem o aumento populacional de carrapatos. Até o momento essa doença foi descrita somente no Brasil. A rangeliose pode causar anemia, icterícia, febre, esplenomegalia, linfadenomegalia, hemorragias no trato gastrintestinal e sangramento persistente pelas bordas e face externa das orelhas, narinas e cavidade oral. O objetivo deste estudo foi avaliar as alterações hematológicas e a resposta da medula óssea em cães infectados experimentalmente com Rangelia vitalii durante a fase aguda da infecção. Foram utilizados 12 cães, separados em dois grupos: grupo A composto por cinco animais saudáveis, e grupo B constituído por sete cães infectados experimentalmente com R. vitalii. Após inoculação, os animais foram monitorados para avaliação da parasitemia por meio de esfregaços sanguíneos de ponta de orelha. No grupo B o parasito foi encontrado no interior de eritrócitos, neutrófilos e monócitos cinco dias pós-inoculação (PI). As coletas de sangue com EDTA para a realização do hemograma foram feitas nos dias 0, 10, 20 e 30 PI. Nos dias 10 e 20 PI também foram realizadas as contagens de reticulócitos. Posteriormente à coleta de sangue, os cães foram anestesiados para coleta dos aspirados de medula óssea para realização do mielograma. Este estudo demostrou redução (P<0,01) no número eritrócitos, hematócrito e hemoglobina no grupo infectado em relação ao controle. No dia 10 PI os animais infectados apresentaram anemia normocítica normocrômica, entretanto, no dia 20 PI foi observada anemia macrocítica hipocrômica e aumento (P<0,05) na amplitude de distribuição eritroide no grupo infectado. Houve redução (P<0,05) nos leucócitos totais no grupo infectado, em relação ao grupo controle, em consequência da redução de neutrófilos e eosinófilos (P<0,05). Também ocorreu aumento (P<0,05) na contagem de linfócitos e monócitos nos cães infectados em relação ao grupo controle. Aumento (P<0,01) na contagem de reticulócitos foi observado no grupo infectado em relação ao grupo controle. No mielograma foi observado uma diminuição (P<0,05) na relação mieloide:eritroide em consequência do aumento dos precursores eritroides (rubriblasto, prorubrícito, rubrícito e metarrubrícito) e diminuição na linhagem mieloide (mielócito, bastonetes e segmentados). Outras células como linfócitos, plasmócitos e macrófagos foram mais frequentes no mielograma no dia 20 PI no grupo infectado em relação ao grupo controle. A partir dos resultados, pode-se concluir que a rangeliose causa anemia hemolítica imunomediada extravascular regenerativa, que coincide com leucopenia por neutropenia e eosinopenia, além, de linfocitose e monocitose. Estes achados que ocorrem na fase aguda da infecção experimental, são semelhantes aos encontrados em casos de infecção natural, nas diferentes fases da doença.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em Medicina VeterináriaUFSMBRMedicina VeterináriaRangelioseAnemiaMielogramaRangeliosisMyelogramCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIAHemograma e mielograma de cães infectados experimentalmente com Rangelia vitaliiHemogram and myelogram dogs infected with experimentally Rangelia vitaliiinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisLopes, Sonia Terezinha dos Anjoshttp://lattes.cnpq.br/8059723754130756Krause, Alexandrehttp://lattes.cnpq.br/7760558908777387Valle, Stella de Fariahttp://lattes.cnpq.br/6159008045542551http://lattes.cnpq.br/1873207812219742França, Raqueli Teresinha5005000000074005003005003000e22bc1c-35eb-49dd-915f-0d5c7bca25b77cab677c-cb90-497f-b3eb-705b8e9594c748b1731f-9fa9-4553-ab4a-18302b9fc514f8043b5d-1ab2-45af-9469-12efe18b0bb5info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações do UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALFRANCA, RAQUELI TERESINHA.pdfapplication/pdf3118287http://repositorio.ufsm.br/bitstream/1/10160/1/FRANCA%2c%20RAQUELI%20TERESINHA.pdf814c1869380828b22013fd9d9309bb48MD51TEXTFRANCA, RAQUELI TERESINHA.pdf.txtFRANCA, RAQUELI TERESINHA.pdf.txtExtracted texttext/plain47844http://repositorio.ufsm.br/bitstream/1/10160/2/FRANCA%2c%20RAQUELI%20TERESINHA.pdf.txt1f4736fd8b6fe8032fb6b8f9592984e8MD52THUMBNAILFRANCA, RAQUELI TERESINHA.pdf.jpgFRANCA, RAQUELI TERESINHA.pdf.jpgIM Thumbnailimage/jpeg4986http://repositorio.ufsm.br/bitstream/1/10160/3/FRANCA%2c%20RAQUELI%20TERESINHA.pdf.jpgd3b42693e581a17cf6750aa50a163d14MD531/101602023-05-25 10:14:41.125oai:repositorio.ufsm.br:1/10160Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2023-05-25T13:14:41Biblioteca Digital de Teses e Dissertações do UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Hemograma e mielograma de cães infectados experimentalmente com Rangelia vitalii |
dc.title.alternative.eng.fl_str_mv |
Hemogram and myelogram dogs infected with experimentally Rangelia vitalii |
title |
Hemograma e mielograma de cães infectados experimentalmente com Rangelia vitalii |
spellingShingle |
Hemograma e mielograma de cães infectados experimentalmente com Rangelia vitalii França, Raqueli Teresinha Rangeliose Anemia Mielograma Rangeliosis Myelogram CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Hemograma e mielograma de cães infectados experimentalmente com Rangelia vitalii |
title_full |
Hemograma e mielograma de cães infectados experimentalmente com Rangelia vitalii |
title_fullStr |
Hemograma e mielograma de cães infectados experimentalmente com Rangelia vitalii |
title_full_unstemmed |
Hemograma e mielograma de cães infectados experimentalmente com Rangelia vitalii |
title_sort |
Hemograma e mielograma de cães infectados experimentalmente com Rangelia vitalii |
author |
França, Raqueli Teresinha |
author_facet |
França, Raqueli Teresinha |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Lopes, Sonia Terezinha dos Anjos |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/8059723754130756 |
dc.contributor.referee1.fl_str_mv |
Krause, Alexandre |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/7760558908777387 |
dc.contributor.referee2.fl_str_mv |
Valle, Stella de Faria |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/6159008045542551 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/1873207812219742 |
dc.contributor.author.fl_str_mv |
França, Raqueli Teresinha |
contributor_str_mv |
Lopes, Sonia Terezinha dos Anjos Krause, Alexandre Valle, Stella de Faria |
dc.subject.por.fl_str_mv |
Rangeliose Anemia Mielograma |
topic |
Rangeliose Anemia Mielograma Rangeliosis Myelogram CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
dc.subject.eng.fl_str_mv |
Rangeliosis Myelogram |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
Rangelia vitalii is a member of the protozoan phylum Apicomplexa, class Sporozoasida, order Piroplasmorida, that causes a disease called rangeliosis in dogs, popularly known as " bleeding plague" or "nambyuvú". The transmission of this parasite occurs through tick and affects dogs in rural and periurban areas especially in hotter times of the year, which favors an increase in ticks population. To date this disease has been described only in Brazil. Rangeliosis is characterized by anemia, jaundice, fever, splenomegaly, generalized lymph node enlargement, hemorrhage along the gastrointestinal tract, and persistent bleeding through the tips of the pinnae, external surface of the ears, nose, and oral cavity. The aim of this study was to evaluate hematologic changes and consequent bone marrow response during the acute phase in dogs experimentally infected with Rangelia vitalii. 12 dogs were, divided into two groups: group A consisted of five healthy animals, and group B consisted of seven dogs experimentally infected with R. vitalii. After inoculation, the animals were monitored for parasitemia by blood smears from the tips of the ear. In group B the parasite was found within erythrocytes, neutrophils and monocytes five days post-inoculation (PI). Blood samples with EDTA for complete blood count (CBC) were performed on days 0, 10, 20 and 30 PI and on days 10 to 20 PI the reticulocyte count. After blood collection, the dogs were anesthetized bone marrow aspiration for the myelogram. This study demonstrated a reduction (P <0.01) in erythrocyte count, hematocrit and hemoglobin in the infected group compared to control. On day 10 PI the infected animals showed a normocytic normochromic anemia, however, on day 20 PI was observed macrocytic hypochromic anemia and increased (P <0.05) in the red cell distribution width (RDW) in the infected group compared to control. A decreased (P <0.05) in total leukocyte count as a result of a decrease in neutrophil and eosinophil count was observed in the infected animals (P <0.05). In the infected group there was also an increase in lymphocyte and monocyte count in comparasion to control (P <0.05). Increased (P <0.01) in the reticulocyte count was observed in the infected group compared to the control group. The myelogram showed a decrease (P <0.05) in myeloid:erythroid ratio as a result of increased erythroid precursors (rubriblast, prorubricyte, rubricyte and metarubricyte) and a decrease in the myeloid lineage (myelocyte, band and segmented). Other cells such as lymphocytes, plasma cells and macrophages were more frequent in bone marrow on day 20 PI in the infected group compared to the control group. Our results, we conclude that rangeliosis can lead to a regenerative, immunomediated, hemolytic anemia, together with leukopenia due to neutropenia and eosinopenia, as well as lymphocytosis and monocytosis. These findings were observed in the acute phase experimental of infection and are similar to those found in cases of natural infection at different stages of the disease. |
publishDate |
2013 |
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2013-02-27 |
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2014-01-22 |
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2014-01-22 |
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info:eu-repo/semantics/masterThesis |
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FRANÇA, Raqueli Teresinha. Hemogram and myelogram dogs infected with experimentally Rangelia vitalii. 2013. 36 f. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2013. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/10160 |
identifier_str_mv |
FRANÇA, Raqueli Teresinha. Hemogram and myelogram dogs infected with experimentally Rangelia vitalii. 2013. 36 f. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2013. |
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http://repositorio.ufsm.br/handle/1/10160 |
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Universidade Federal de Santa Maria |
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UFSM |
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Universidade Federal de Santa Maria |
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