Parâmetros toxicológicos em piavas (Leporinus obtusidens) e jundiás (Rhamdia quelen) após exposição a uma formulação comercial de glyphosate
Ano de defesa: | 2008 |
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Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , , , |
Tipo de documento: | Tese |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Santa Maria
|
Programa de Pós-Graduação: |
Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica
|
Departamento: |
Bioquímica
|
País: |
BR
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://repositorio.ufsm.br/handle/1/4393 |
Resumo: | The commercial formulations of glyphosate herbicide have been widely used in agriculture and pisciculture for controlling plant weeds. Fish can be affected when drainage water reaches water courses, causing a disturbance in the aquatic ecosystem. Therefore, the aim of this study was to investigate whether exposure to a commercial formulation of glyphosate affects toxicological parameters in piavas (Leporinus obtusidens) and jundiás (Rhamdia quelen). Piavas were exposed to: 0 (control), 3, 6, 10 or 20 mg/L of glyphosate (480 g/L) and jundiás to 0 (control), 0.2 or 0.4 mg/L, both for 96 h. Enzymatic (AChE), hematological (hematocrit, hemoglobin and erythrocyte and leucocyte count) and metabolic (glucose, glycogen, lactate, protein and ammonia) parameters were analyzed in different tissues of these species. In addition, oxidative stress parameters, such as activity of the antioxidant enzyme catalase, protein carbonilation and TBARS levels were analyzed. The results showed that brain acetylcholinesterase (AChE) activity decreased significantly in both species exposed, but there was no significant change in the muscle tissue. There was a decrease in hematological parameters in blood of piavas. After exposure to this herbicide, both species demonstrated metabolic disorders. In piavas, there was a reduction of glycogen and an increase of glucose in hepatic tissue, and a reduction of muscle glycogen and glucose. Lactate and protein showed a decrease in hepatic tissue after exposure to all concentrations of the herbicide, but in muscle tissue there was an increase in these metabolites. The levels of ammonia increased in both tissues after exposure, varying with the glyphosate concentration. In jundiás, there was an increase of hepatic glycogen, but a reduction of muscle glycogen at both concentrations tested. Glucose showed a decrease in liver and increase in muscle. After exposure to this herbicide, the levels of lactate were increased in both tissues. The levels of protein were increased in hepatic tissue and decreased in muscle tissue, while the levels of ammonia showed an increase at both herbicide concentrations in both tissues tested. Catalase activity showed an increase in jundiás, but not in piavas. TBARS levels showed an elevation in muscle tissue in jundiás while in piavas there was an increase in hepatic tissue. An increase in protein carbonyl formation in liver of jundiás was observed. In addition, there was an increase in mucus parameters in piavas. These results indicate that the parameters measured may be good indicators of herbicide contamination in piavas and jundiás of the southern region of Brazil. |
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2017-04-242017-04-242008-02-15GLUSCZAK, Lissandra. Toxicological parameters in piava (Leporinus obtusidens) and jundiá (Rhamdia quelen) after exposure to commercial formulation of glyphosate. 2008. 94 f. Tese (Doutorado em Bioquímica) - Universidade Federal de Santa Maria, Santa Maria, 2008.http://repositorio.ufsm.br/handle/1/4393The commercial formulations of glyphosate herbicide have been widely used in agriculture and pisciculture for controlling plant weeds. Fish can be affected when drainage water reaches water courses, causing a disturbance in the aquatic ecosystem. Therefore, the aim of this study was to investigate whether exposure to a commercial formulation of glyphosate affects toxicological parameters in piavas (Leporinus obtusidens) and jundiás (Rhamdia quelen). Piavas were exposed to: 0 (control), 3, 6, 10 or 20 mg/L of glyphosate (480 g/L) and jundiás to 0 (control), 0.2 or 0.4 mg/L, both for 96 h. Enzymatic (AChE), hematological (hematocrit, hemoglobin and erythrocyte and leucocyte count) and metabolic (glucose, glycogen, lactate, protein and ammonia) parameters were analyzed in different tissues of these species. In addition, oxidative stress parameters, such as activity of the antioxidant enzyme catalase, protein carbonilation and TBARS levels were analyzed. The results showed that brain acetylcholinesterase (AChE) activity decreased significantly in both species exposed, but there was no significant change in the muscle tissue. There was a decrease in hematological parameters in blood of piavas. After exposure to this herbicide, both species demonstrated metabolic disorders. In piavas, there was a reduction of glycogen and an increase of glucose in hepatic tissue, and a reduction of muscle glycogen and glucose. Lactate and protein showed a decrease in hepatic tissue after exposure to all concentrations of the herbicide, but in muscle tissue there was an increase in these metabolites. The levels of ammonia increased in both tissues after exposure, varying with the glyphosate concentration. In jundiás, there was an increase of hepatic glycogen, but a reduction of muscle glycogen at both concentrations tested. Glucose showed a decrease in liver and increase in muscle. After exposure to this herbicide, the levels of lactate were increased in both tissues. The levels of protein were increased in hepatic tissue and decreased in muscle tissue, while the levels of ammonia showed an increase at both herbicide concentrations in both tissues tested. Catalase activity showed an increase in jundiás, but not in piavas. TBARS levels showed an elevation in muscle tissue in jundiás while in piavas there was an increase in hepatic tissue. An increase in protein carbonyl formation in liver of jundiás was observed. In addition, there was an increase in mucus parameters in piavas. These results indicate that the parameters measured may be good indicators of herbicide contamination in piavas and jundiás of the southern region of Brazil.As formulações comerciais do herbicida glyphosate têm sido amplamente utilizadas na agricultura e na piscicultura para controle de plantas daninhas. Os peixes podem ser afetados quando as águas de drenagem atingem os cursos d água, acarretando um desequilíbrio no ecossistema aquático. Sendo assim, o objetivo deste estudo foi avaliar o efeito de uma formulação comercial de glyphosate sobre parâmetros toxicológicos em piavas (Leporinus obtusidens) e jundiás (Rhamdia quelen). Piavas foram expostas à: 0 (controle), 3, 6, 10 ou 20 mg/L de glyphosate (480 g/L) e os jundiás à 0 (controle), 0,2 ou 0,4 mg/L , ambos por 96 h. Os parâmetros analisados foram enzimáticos (AChE), hematológicos (hematócrito, hemoglobina, contagem de eritrócitos e de leucócitos) e metabólicos (glicose, glicogênio, lactato, proteína e amônia) em diferentes tecidos destas espécies. Ademais, analisaram-se parâmetros de estresse oxidativo, como a atividade da enzima antioxidante catalase, carbonilação de proteínas e níveis de TBARS. Os resultados mostraram que a atividade da acetilcolinesterase (AChE) cerebral diminuiu significativamente em ambas as espécies expostas, porém no tecido muscular não se observaram alterações significativas. Houve um decréscimo nos parâmetros hematológicos no sangue de piavas expostas. Após exposição ao glyphosate, as duas espécies demonstraram desordens metabólicas. Em piavas, ocorreu uma redução nos níveis de glicogênio e um aumento nos níveis de glicose no tecido hepático, porém uma significante redução do glicogênio e glicose muscular. As concentrações de lactato e proteína apresentaram uma diminuição no tecido hepático após exposição a todas as concentrações deste herbicida, porém no tecido muscular houve um aumento destes metabólitos. Os níveis de amônia aumentaram em ambos tecidos após exposição às diferentes concentrações do glyphosate. Em jundiás, a glicose esteve reduzida no fígado e aumentada no músculo. Houve um aumento do glicogênio hepático, mas uma redução do glicogênio muscular em ambas as concentrações testadas. Após exposição a este herbicida, os níveis de lactato estavam aumentados em ambos tecidos. Os níveis de proteína estavam aumentados no tecido hepático e diminuídos no tecido muscular, enquanto os níveis de amônia estavam aumentados em ambas as concentrações e tecidos testados. A atividade da enzima antioxidante catalase apresentou um aumento em jundiás expostos, porém em piavas não houve alterações. Os níveis de TBARS em jundiás mostraram uma elevação no tecido muscular enquanto em piavas houve um aumento no tecido hepático. Observou-se um aumento na formação de proteína carbonila em fígado de jundiás. Além disso, houve aumento nos parâmetros do muco (glicose e proteína) em piavas expostas. Estes resultados indicam que os parâmetros medidos podem ser bons indicadores da contaminação deste herbicida em piavas e jundiás da região Sul.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de Santa MariaPrograma de Pós-Graduação em Ciências Biológicas: Bioquímica ToxicológicaUFSMBRBioquímicaGlyphosatePiava (Leporinus obtusidens)Jundiá (Rhamdia quelen)EnzimologiaHematologiaMetabolismoEstresse oxidativoMucoGlyphosatePiava (Leporinus obtusidens)Jundiá (Rhamdia quelen)EnzymologyHematologicalMetabolismAntioxidantMucusCNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICAParâmetros toxicológicos em piavas (Leporinus obtusidens) e jundiás (Rhamdia quelen) após exposição a uma formulação comercial de glyphosateToxicological parameters in piava (Leporinus obtusidens) and jundiá (Rhamdia quelen) after exposure to commercial formulation of glyphosateinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisLoro, Vania Luciahttp://lattes.cnpq.br/6392817606416780Ferreira, Julianohttp://lattes.cnpq.br/2694197910478313Barcellos, Leonardo José Gilhttp://lattes.cnpq.br/4504482634911546Avila, Luis Antonio dehttp://lattes.cnpq.br/5245663326931142Schetinger, Maria Rosa Chitolinahttp://lattes.cnpq.br/0681050170546708Glusczak, Lissandra200800000002400300300300500500500b0918185-c6b7-49df-b8d6-d245d73535b8d2a68218-a822-4a8a-b6a0-4634d76dd90f1282ebc7-dffe-4b16-9e7c-cd9ddd79f214de1fbfb1-090e-4afb-99c2-1e8307678fbc38cfc408-a8aa-4b27-a204-f38d9d86364498300743-ff68-46b6-b7c0-7145a94b913dinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações do UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMORIGINALLISSANDRA GLUSCZAK.pdfapplication/pdf2529011http://repositorio.ufsm.br/bitstream/1/4393/1/LISSANDRA%20GLUSCZAK.pdf71a89c30b1a13e76cf038a591d0bdcb3MD51TEXTLISSANDRA GLUSCZAK.pdf.txtLISSANDRA GLUSCZAK.pdf.txtExtracted texttext/plain125170http://repositorio.ufsm.br/bitstream/1/4393/2/LISSANDRA%20GLUSCZAK.pdf.txt6a7dbdc82ab4c8297ea0492ffe4c8411MD52THUMBNAILLISSANDRA GLUSCZAK.pdf.jpgLISSANDRA GLUSCZAK.pdf.jpgIM Thumbnailimage/jpeg6363http://repositorio.ufsm.br/bitstream/1/4393/3/LISSANDRA%20GLUSCZAK.pdf.jpga4fb8136468fcce9460f41ca150af93fMD531/43932022-09-09 09:05:58.965oai:repositorio.ufsm.br:1/4393Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2022-09-09T12:05:58Biblioteca Digital de Teses e Dissertações do UFSM - Universidade Federal de Santa Maria (UFSM)false |
dc.title.por.fl_str_mv |
Parâmetros toxicológicos em piavas (Leporinus obtusidens) e jundiás (Rhamdia quelen) após exposição a uma formulação comercial de glyphosate |
dc.title.alternative.eng.fl_str_mv |
Toxicological parameters in piava (Leporinus obtusidens) and jundiá (Rhamdia quelen) after exposure to commercial formulation of glyphosate |
title |
Parâmetros toxicológicos em piavas (Leporinus obtusidens) e jundiás (Rhamdia quelen) após exposição a uma formulação comercial de glyphosate |
spellingShingle |
Parâmetros toxicológicos em piavas (Leporinus obtusidens) e jundiás (Rhamdia quelen) após exposição a uma formulação comercial de glyphosate Glusczak, Lissandra Glyphosate Piava (Leporinus obtusidens) Jundiá (Rhamdia quelen) Enzimologia Hematologia Metabolismo Estresse oxidativo Muco Glyphosate Piava (Leporinus obtusidens) Jundiá (Rhamdia quelen) Enzymology Hematological Metabolism Antioxidant Mucus CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
title_short |
Parâmetros toxicológicos em piavas (Leporinus obtusidens) e jundiás (Rhamdia quelen) após exposição a uma formulação comercial de glyphosate |
title_full |
Parâmetros toxicológicos em piavas (Leporinus obtusidens) e jundiás (Rhamdia quelen) após exposição a uma formulação comercial de glyphosate |
title_fullStr |
Parâmetros toxicológicos em piavas (Leporinus obtusidens) e jundiás (Rhamdia quelen) após exposição a uma formulação comercial de glyphosate |
title_full_unstemmed |
Parâmetros toxicológicos em piavas (Leporinus obtusidens) e jundiás (Rhamdia quelen) após exposição a uma formulação comercial de glyphosate |
title_sort |
Parâmetros toxicológicos em piavas (Leporinus obtusidens) e jundiás (Rhamdia quelen) após exposição a uma formulação comercial de glyphosate |
author |
Glusczak, Lissandra |
author_facet |
Glusczak, Lissandra |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Loro, Vania Lucia |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/6392817606416780 |
dc.contributor.referee1.fl_str_mv |
Ferreira, Juliano |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/2694197910478313 |
dc.contributor.referee2.fl_str_mv |
Barcellos, Leonardo José Gil |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/4504482634911546 |
dc.contributor.referee3.fl_str_mv |
Avila, Luis Antonio de |
dc.contributor.referee3Lattes.fl_str_mv |
http://lattes.cnpq.br/5245663326931142 |
dc.contributor.referee4.fl_str_mv |
Schetinger, Maria Rosa Chitolina |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/0681050170546708 |
dc.contributor.author.fl_str_mv |
Glusczak, Lissandra |
contributor_str_mv |
Loro, Vania Lucia Ferreira, Juliano Barcellos, Leonardo José Gil Avila, Luis Antonio de Schetinger, Maria Rosa Chitolina |
dc.subject.por.fl_str_mv |
Glyphosate Piava (Leporinus obtusidens) Jundiá (Rhamdia quelen) Enzimologia Hematologia Metabolismo Estresse oxidativo Muco |
topic |
Glyphosate Piava (Leporinus obtusidens) Jundiá (Rhamdia quelen) Enzimologia Hematologia Metabolismo Estresse oxidativo Muco Glyphosate Piava (Leporinus obtusidens) Jundiá (Rhamdia quelen) Enzymology Hematological Metabolism Antioxidant Mucus CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
dc.subject.eng.fl_str_mv |
Glyphosate Piava (Leporinus obtusidens) Jundiá (Rhamdia quelen) Enzymology Hematological Metabolism Antioxidant Mucus |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA |
description |
The commercial formulations of glyphosate herbicide have been widely used in agriculture and pisciculture for controlling plant weeds. Fish can be affected when drainage water reaches water courses, causing a disturbance in the aquatic ecosystem. Therefore, the aim of this study was to investigate whether exposure to a commercial formulation of glyphosate affects toxicological parameters in piavas (Leporinus obtusidens) and jundiás (Rhamdia quelen). Piavas were exposed to: 0 (control), 3, 6, 10 or 20 mg/L of glyphosate (480 g/L) and jundiás to 0 (control), 0.2 or 0.4 mg/L, both for 96 h. Enzymatic (AChE), hematological (hematocrit, hemoglobin and erythrocyte and leucocyte count) and metabolic (glucose, glycogen, lactate, protein and ammonia) parameters were analyzed in different tissues of these species. In addition, oxidative stress parameters, such as activity of the antioxidant enzyme catalase, protein carbonilation and TBARS levels were analyzed. The results showed that brain acetylcholinesterase (AChE) activity decreased significantly in both species exposed, but there was no significant change in the muscle tissue. There was a decrease in hematological parameters in blood of piavas. After exposure to this herbicide, both species demonstrated metabolic disorders. In piavas, there was a reduction of glycogen and an increase of glucose in hepatic tissue, and a reduction of muscle glycogen and glucose. Lactate and protein showed a decrease in hepatic tissue after exposure to all concentrations of the herbicide, but in muscle tissue there was an increase in these metabolites. The levels of ammonia increased in both tissues after exposure, varying with the glyphosate concentration. In jundiás, there was an increase of hepatic glycogen, but a reduction of muscle glycogen at both concentrations tested. Glucose showed a decrease in liver and increase in muscle. After exposure to this herbicide, the levels of lactate were increased in both tissues. The levels of protein were increased in hepatic tissue and decreased in muscle tissue, while the levels of ammonia showed an increase at both herbicide concentrations in both tissues tested. Catalase activity showed an increase in jundiás, but not in piavas. TBARS levels showed an elevation in muscle tissue in jundiás while in piavas there was an increase in hepatic tissue. An increase in protein carbonyl formation in liver of jundiás was observed. In addition, there was an increase in mucus parameters in piavas. These results indicate that the parameters measured may be good indicators of herbicide contamination in piavas and jundiás of the southern region of Brazil. |
publishDate |
2008 |
dc.date.issued.fl_str_mv |
2008-02-15 |
dc.date.accessioned.fl_str_mv |
2017-04-24 |
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2017-04-24 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/doctoralThesis |
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doctoralThesis |
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publishedVersion |
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GLUSCZAK, Lissandra. Toxicological parameters in piava (Leporinus obtusidens) and jundiá (Rhamdia quelen) after exposure to commercial formulation of glyphosate. 2008. 94 f. Tese (Doutorado em Bioquímica) - Universidade Federal de Santa Maria, Santa Maria, 2008. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufsm.br/handle/1/4393 |
identifier_str_mv |
GLUSCZAK, Lissandra. Toxicological parameters in piava (Leporinus obtusidens) and jundiá (Rhamdia quelen) after exposure to commercial formulation of glyphosate. 2008. 94 f. Tese (Doutorado em Bioquímica) - Universidade Federal de Santa Maria, Santa Maria, 2008. |
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http://repositorio.ufsm.br/handle/1/4393 |
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por |
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200800000002 |
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Universidade Federal de Santa Maria |
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Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica |
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UFSM |
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Bioquímica |
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Universidade Federal de Santa Maria |
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