Efeito do cobre e do selenito de sódio no estresse oxidativo, proteínas séricas e carga parasitária de cordeiros infectados experimentalmente por Haemonchus contortus

• Ano de defesa: 2011
• Autor(a) principal: Fausto, Guilherme Costa
• Orientador(a): Leal, Marta Lizandra do Rêgo
• Banca de defesa: Berne, Maria Elisabeth Aires , Fialho, Sergio da Silva
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Santa Maria
• Programa de Pós-Graduação: Programa de Pós-Graduação em Medicina Veterinária
• Departamento: Medicina Veterinária
• País: BR
• Palavras-chave em Português: Codeiros; Estresse oxidativo; Proteínas séricas; H.contortus
• Palavras-chave em Inglês: Lambs; Oxidative stress; Serum proteins; H.contortus
• Área do conhecimento CNPq: CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA
• Link de acesso: http://repositorio.ufsm.br/handle/1/10096

This study aimed to evaluate the oxidative status and electrophoresis of sheep experimentally infected with Haemonchus contortus and supplemented with selenium and copper. We used 28 Corriedale and Texel crossbred lambs were divided into four groups with seven animals each: G1 consisted of animals infected with larvae of G2 were infected with larvae in lambs supplemented with 0.2 mg / kg body weight (LW) of sodium selenite intramuscular (IM), G3 were infected with larvae and supplemented with 3.5 mg / kg BW of copper by subcutaneous (SC); G4 consisted of animals infected with larvae and supplemented with 0.2 mg / kg BW IM sodium selenite and 3.5 mg / kg LW SC copper. All groups were infected orally with 500 L3 larvae of Haemonchus contortus per animal every other day for a period of twenty days from the day zero. The blood samples used for biochemical analysis were collected on days zero (T0), 20 (T1), 40 (T2), 60 (T3) and 80 (T4) by jugular venipuncture using vacutainer tubes®. Weights and determination of eggs per gram of feces of animals occurred in these experimental times. The gradual increase in catalase and TBARS values in all groups showed oxidative stress and inflammation due to parasitic infection. There was an increased activity of GSH-Px in G2 at T0, T1 and T4 as compared to G1 and T4 when compared with G3. Selenium supplementation promoted a greater antioxidant activity in parasitized animals. The use of copper caused an increase in weight gain of experimental animals. The use of sodium selenite associated with copper was more effective in increasing levels of total protein, albumin and gamma globulin in T4, which were also obtained the lowest FEC and worm burden.