Determinação de resíduos de agrotóxicos em fisalis (Physalis peruaviana L.) empregando QuEChERS modificado e UHPLC-MS/MS

Detalhes bibliográficos
Ano de defesa: 2016
Autor(a) principal: Munoz, Natalia Cadavid lattes
Orientador(a): Zanella, Renato lattes
Banca de defesa: Gonçalves, Fabio Ferreira, Prestes, Osmar Damian
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal de Santa Maria
Centro de Ciências Naturais e Exatas
Programa de Pós-Graduação: Programa de Pós-Graduação em Química
Departamento: Química
País: Brasil
Palavras-chave em Português:
Agrotóxicos
Fisalis
Alimentos
QuEChERS
UHPLC-MS/MS
Palavras-chave em Inglês:
Pesticides
Goldenberry
Food
Área do conhecimento CNPq:
CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA
Link de acesso: http://repositorio.ufsm.br/handle/1/22364
Resumo: In this study a fast and effective multiresidue method for the determination of 42 pesticides in goldenberry was developed and validated. A modified QuEChERS method was established for sample preparation followed by ultra-high-performance liquid chromatographic–tandem mass spectrometry (UHPLC–MS/MS) determination with electrospray ionization in a triple quadrupole system. Validation results were satisfactory, since the proposed method presented recoveries between 70 and 114% with relative standard deviations (RSD) <20% for blank samples spiked from 5 to 25 μg kg-1. The method limits of detection and of quantification were 1.5 and 5 μg kg-1, respectively. Matrix effect ranged from -32 to 218% and was compensated using matrix matched calibration. Method linearity was established from 2.5 to 100 μg kg-1 with r2 ≥0.99. The proposed method combines the advantages of a simple and fast sample preparation step by a modified QuEChERS method with the high selectivity and sensitivity of the UHPLC-MS/MS system using selected reaction monitoring. Method was successfully applied to real samples, proving to be an efficient alternative for routine analysis. Residues of five pesticides were found in the samples evaluated.
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spelling 2021-10-08T12:10:04Z2021-10-08T12:10:04Z2016-02-29http://repositorio.ufsm.br/handle/1/22364In this study a fast and effective multiresidue method for the determination of 42 pesticides in goldenberry was developed and validated. A modified QuEChERS method was established for sample preparation followed by ultra-high-performance liquid chromatographic–tandem mass spectrometry (UHPLC–MS/MS) determination with electrospray ionization in a triple quadrupole system. Validation results were satisfactory, since the proposed method presented recoveries between 70 and 114% with relative standard deviations (RSD) <20% for blank samples spiked from 5 to 25 μg kg-1. The method limits of detection and of quantification were 1.5 and 5 μg kg-1, respectively. Matrix effect ranged from -32 to 218% and was compensated using matrix matched calibration. Method linearity was established from 2.5 to 100 μg kg-1 with r2 ≥0.99. The proposed method combines the advantages of a simple and fast sample preparation step by a modified QuEChERS method with the high selectivity and sensitivity of the UHPLC-MS/MS system using selected reaction monitoring. Method was successfully applied to real samples, proving to be an efficient alternative for routine analysis. Residues of five pesticides were found in the samples evaluated.O trabalho foi desenvolvido e validado um método multirresíduo rápido e eficaz para a determinação de 42 agrotóxicos em fisalis. O método QuEChERS modificado foi utilizado para o preparo de amostra seguido pela determinação por cromatografia líquida de ultra-alta eficiência acoplada à espectrometria de massas em série (UHPLC-MS/MS), com modo de ionização por eletronebulização empregando analisador triplo quadrupolo. Os resultados de validação foram satisfatórios, uma vez que o método apresentou recuperações entre 70 e 114%, com desvio padrão relativo (RSD) <20% para amostras branco fortificadas de 5 a 25 μg kg-1. Os limites de detecção e de quantificação do método foram de 1,5 e 5 μg kg-1, respectivamente. O efeito matriz variou de -32 a 218% e foi corrigido utilizando curva analítica na matriz. A linearidade do método foi estabelecida de 2,5 a 100 μg kg-1, com r2 ≥0.99. O método proposto combina as vantagens de uma etapa de preparo de amostra simples e rápida, aliado à elevada seletividade e sensibilidade do sistema UHPLC-MS/MS empregando monitoramento de reações selecionadas. O método foi aplicado com sucesso em amostras reais, provando ser uma alternativa eficiente para a análise de rotina. Cinco resíduos de pesticidas foram encontrados nas amostras avaliadas.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESporUniversidade Federal de Santa MariaCentro de Ciências Naturais e ExatasPrograma de Pós-Graduação em QuímicaUFSMBrasilQuímicaAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessAgrotóxicosFisalisAlimentosQuEChERSUHPLC-MS/MSPesticidesGoldenberryFoodCNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICADeterminação de resíduos de agrotóxicos em fisalis (Physalis peruaviana L.) empregando QuEChERS modificado e UHPLC-MS/MSDetermination of pesticide residues in goldenberry (Physalis peruaviana L.) BY modified QuEChERS method and UHPLC-MS/MSinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisZanella, Renatohttp://lattes.cnpq.br/2541865299438479Gonçalves, Fabio FerreiraPrestes, Osmar Damianhttp://lattes.cnpq.br/2678416215512334Munoz, Natalia Cadavid1006000000006006006006006000aa2baab-94e8-4d3d-bbb2-85495ba77db5e65464ea-4459-435b-94b2-7d358d84a8989e2a8b3c-033e-4402-a719-b5bd327df04b4eef7b33-113c-470f-8729-f99c250df028reponame:Biblioteca Digital de Teses e Dissertações do UFSMinstname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMLICENSElicense.txtlicense.txttext/plain; charset=utf-81956http://repositorio.ufsm.br/bitstream/1/22364/3/license.txt2f0571ecee68693bd5cd3f17c1e075dfMD53ORIGINALDIS_PPGQUIMICA_2016_MUNOZ_NATALIA.pdfDIS_PPGQUIMICA_2016_MUNOZ_NATALIA.pdfDissertaçãoapplication/pdf1637885http://repositorio.ufsm.br/bitstream/1/22364/1/DIS_PPGQUIMICA_2016_MUNOZ_NATALIA.pdf8a41698550fa74b567f45ceec9cf9fd6MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8805http://repositorio.ufsm.br/bitstream/1/22364/2/license_rdf4460e5956bc1d1639be9ae6146a50347MD52TEXTDIS_PPGQUIMICA_2016_MUNOZ_NATALIA.pdf.txtDIS_PPGQUIMICA_2016_MUNOZ_NATALIA.pdf.txtExtracted texttext/plain121442http://repositorio.ufsm.br/bitstream/1/22364/4/DIS_PPGQUIMICA_2016_MUNOZ_NATALIA.pdf.txt3d5343df3f5676a366af68de7eecc97dMD54THUMBNAILDIS_PPGQUIMICA_2016_MUNOZ_NATALIA.pdf.jpgDIS_PPGQUIMICA_2016_MUNOZ_NATALIA.pdf.jpgIM Thumbnailimage/jpeg4523http://repositorio.ufsm.br/bitstream/1/22364/5/DIS_PPGQUIMICA_2016_MUNOZ_NATALIA.pdf.jpgb0e9468b4f10f90cac4ae99fd7e21bfcMD551/223642021-10-09 03:03:08.38oai:repositorio.ufsm.br:1/22364TElDRU7Dh0EgREUgRElTVFJJQlVJw4fDg08gTsODTy1FWENMVVNJVkEKCkNvbSBhIGFwcmVzZW50YcOnw6NvIGRlc3RhIGxpY2Vuw6dhLCB2b2PDqiAobyBhdXRvciAoZXMpIG91IG8gdGl0dWxhciBkb3MgZGlyZWl0b3MgZGUgYXV0b3IpIGNvbmNlZGUgw6AgVW5pdmVyc2lkYWRlCkZlZGVyYWwgZGUgU2FudGEgTWFyaWEgKFVGU00pIG8gZGlyZWl0byBuw6NvLWV4Y2x1c2l2byBkZSByZXByb2R1emlyLCAgdHJhZHV6aXIgKGNvbmZvcm1lIGRlZmluaWRvIGFiYWl4byksIGUvb3UKZGlzdHJpYnVpciBhIHN1YSB0ZXNlIG91IGRpc3NlcnRhw6fDo28gKGluY2x1aW5kbyBvIHJlc3VtbykgcG9yIHRvZG8gbyBtdW5kbyBubyBmb3JtYXRvIGltcHJlc3NvIGUgZWxldHLDtG5pY28gZQplbSBxdWFscXVlciBtZWlvLCBpbmNsdWluZG8gb3MgZm9ybWF0b3Mgw6F1ZGlvIG91IHbDrWRlby4KClZvY8OqIGNvbmNvcmRhIHF1ZSBhIFVGU00gcG9kZSwgc2VtIGFsdGVyYXIgbyBjb250ZcO6ZG8sIHRyYW5zcG9yIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbwpwYXJhIHF1YWxxdWVyIG1laW8gb3UgZm9ybWF0byBwYXJhIGZpbnMgZGUgcHJlc2VydmHDp8Ojby4KClZvY8OqIHRhbWLDqW0gY29uY29yZGEgcXVlIGEgVUZTTSBwb2RlIG1hbnRlciBtYWlzIGRlIHVtYSBjw7NwaWEgYSBzdWEgdGVzZSBvdQpkaXNzZXJ0YcOnw6NvIHBhcmEgZmlucyBkZSBzZWd1cmFuw6dhLCBiYWNrLXVwIGUgcHJlc2VydmHDp8Ojby4KClZvY8OqIGRlY2xhcmEgcXVlIGEgc3VhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbyDDqSBvcmlnaW5hbCBlIHF1ZSB2b2PDqiB0ZW0gbyBwb2RlciBkZSBjb25jZWRlciBvcyBkaXJlaXRvcyBjb250aWRvcwpuZXN0YSBsaWNlbsOnYS4gVm9jw6ogdGFtYsOpbSBkZWNsYXJhIHF1ZSBvIGRlcMOzc2l0byBkYSBzdWEgdGVzZSBvdSBkaXNzZXJ0YcOnw6NvIG7Do28sIHF1ZSBzZWphIGRlIHNldQpjb25oZWNpbWVudG8sIGluZnJpbmdlIGRpcmVpdG9zIGF1dG9yYWlzIGRlIG5pbmd1w6ltLgoKQ2FzbyBhIHN1YSB0ZXNlIG91IGRpc3NlcnRhw6fDo28gY29udGVuaGEgbWF0ZXJpYWwgcXVlIHZvY8OqIG7Do28gcG9zc3VpIGEgdGl0dWxhcmlkYWRlIGRvcyBkaXJlaXRvcyBhdXRvcmFpcywgdm9jw6oKZGVjbGFyYSBxdWUgb2J0ZXZlIGEgcGVybWlzc8OjbyBpcnJlc3RyaXRhIGRvIGRldGVudG9yIGRvcyBkaXJlaXRvcyBhdXRvcmFpcyBwYXJhIGNvbmNlZGVyIMOgIFVGU00Kb3MgZGlyZWl0b3MgYXByZXNlbnRhZG9zIG5lc3RhIGxpY2Vuw6dhLCBlIHF1ZSBlc3NlIG1hdGVyaWFsIGRlIHByb3ByaWVkYWRlIGRlIHRlcmNlaXJvcyBlc3TDoSBjbGFyYW1lbnRlCmlkZW50aWZpY2FkbyBlIHJlY29uaGVjaWRvIG5vIHRleHRvIG91IG5vIGNvbnRlw7pkbyBkYSB0ZXNlIG91IGRpc3NlcnRhw6fDo28gb3JhIGRlcG9zaXRhZGEuCgpDQVNPIEEgVEVTRSBPVSBESVNTRVJUQcOHw4NPIE9SQSBERVBPU0lUQURBIFRFTkhBIFNJRE8gUkVTVUxUQURPIERFIFVNIFBBVFJPQ8ONTklPIE9VCkFQT0lPIERFIFVNQSBBR8OKTkNJQSBERSBGT01FTlRPIE9VIE9VVFJPIE9SR0FOSVNNTyBRVUUgTsODTyBTRUpBIEEgVUZTTQosIFZPQ8OKIERFQ0xBUkEgUVVFIFJFU1BFSVRPVSBUT0RPUyBFIFFVQUlTUVVFUiBESVJFSVRPUyBERSBSRVZJU8ODTyBDT01PClRBTULDiU0gQVMgREVNQUlTIE9CUklHQcOHw5VFUyBFWElHSURBUyBQT1IgQ09OVFJBVE8gT1UgQUNPUkRPLgoKQSBVRlNNIHNlIGNvbXByb21ldGUgYSBpZGVudGlmaWNhciBjbGFyYW1lbnRlIG8gc2V1IG5vbWUgKHMpIG91IG8ocykgbm9tZShzKSBkbyhzKQpkZXRlbnRvcihlcykgZG9zIGRpcmVpdG9zIGF1dG9yYWlzIGRhIHRlc2Ugb3UgZGlzc2VydGHDp8OjbywgZSBuw6NvIGZhcsOhIHF1YWxxdWVyIGFsdGVyYcOnw6NvLCBhbMOpbSBkYXF1ZWxhcwpjb25jZWRpZGFzIHBvciBlc3RhIGxpY2Vuw6dhLgoKBiblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufsm.br/ONGhttps://repositorio.ufsm.br/oai/requestatendimento.sib@ufsm.br||tedebc@gmail.comopendoar:2021-10-09T06:03:08Biblioteca Digital de Teses e Dissertações do UFSM - Universidade Federal de Santa Maria (UFSM)false
dc.title.por.fl_str_mv Determinação de resíduos de agrotóxicos em fisalis (Physalis peruaviana L.) empregando QuEChERS modificado e UHPLC-MS/MS
dc.title.alternative.eng.fl_str_mv Determination of pesticide residues in goldenberry (Physalis peruaviana L.) BY modified QuEChERS method and UHPLC-MS/MS
title Determinação de resíduos de agrotóxicos em fisalis (Physalis peruaviana L.) empregando QuEChERS modificado e UHPLC-MS/MS
spellingShingle Determinação de resíduos de agrotóxicos em fisalis (Physalis peruaviana L.) empregando QuEChERS modificado e UHPLC-MS/MS
Munoz, Natalia Cadavid
Agrotóxicos
Fisalis
Alimentos
QuEChERS
UHPLC-MS/MS
Pesticides
Goldenberry
Food
CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA
title_short Determinação de resíduos de agrotóxicos em fisalis (Physalis peruaviana L.) empregando QuEChERS modificado e UHPLC-MS/MS
title_full Determinação de resíduos de agrotóxicos em fisalis (Physalis peruaviana L.) empregando QuEChERS modificado e UHPLC-MS/MS
title_fullStr Determinação de resíduos de agrotóxicos em fisalis (Physalis peruaviana L.) empregando QuEChERS modificado e UHPLC-MS/MS
title_full_unstemmed Determinação de resíduos de agrotóxicos em fisalis (Physalis peruaviana L.) empregando QuEChERS modificado e UHPLC-MS/MS
title_sort Determinação de resíduos de agrotóxicos em fisalis (Physalis peruaviana L.) empregando QuEChERS modificado e UHPLC-MS/MS
author Munoz, Natalia Cadavid
author_facet Munoz, Natalia Cadavid
author_role author
dc.contributor.advisor1.fl_str_mv Zanella, Renato
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/2541865299438479
dc.contributor.referee1.fl_str_mv Gonçalves, Fabio Ferreira
dc.contributor.referee2.fl_str_mv Prestes, Osmar Damian
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/2678416215512334
dc.contributor.author.fl_str_mv Munoz, Natalia Cadavid
contributor_str_mv Zanella, Renato
Gonçalves, Fabio Ferreira
Prestes, Osmar Damian
dc.subject.por.fl_str_mv Agrotóxicos
Fisalis
Alimentos
QuEChERS
UHPLC-MS/MS
topic Agrotóxicos
Fisalis
Alimentos
QuEChERS
UHPLC-MS/MS
Pesticides
Goldenberry
Food
CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA
dc.subject.eng.fl_str_mv Pesticides
Goldenberry
Food
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA
description In this study a fast and effective multiresidue method for the determination of 42 pesticides in goldenberry was developed and validated. A modified QuEChERS method was established for sample preparation followed by ultra-high-performance liquid chromatographic–tandem mass spectrometry (UHPLC–MS/MS) determination with electrospray ionization in a triple quadrupole system. Validation results were satisfactory, since the proposed method presented recoveries between 70 and 114% with relative standard deviations (RSD) <20% for blank samples spiked from 5 to 25 μg kg-1. The method limits of detection and of quantification were 1.5 and 5 μg kg-1, respectively. Matrix effect ranged from -32 to 218% and was compensated using matrix matched calibration. Method linearity was established from 2.5 to 100 μg kg-1 with r2 ≥0.99. The proposed method combines the advantages of a simple and fast sample preparation step by a modified QuEChERS method with the high selectivity and sensitivity of the UHPLC-MS/MS system using selected reaction monitoring. Method was successfully applied to real samples, proving to be an efficient alternative for routine analysis. Residues of five pesticides were found in the samples evaluated.
publishDate 2016
dc.date.issued.fl_str_mv 2016-02-29
dc.date.accessioned.fl_str_mv 2021-10-08T12:10:04Z
dc.date.available.fl_str_mv 2021-10-08T12:10:04Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufsm.br/handle/1/22364
url http://repositorio.ufsm.br/handle/1/22364
dc.language.iso.fl_str_mv por
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dc.rights.driver.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
Centro de Ciências Naturais e Exatas
dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Química
dc.publisher.initials.fl_str_mv UFSM
dc.publisher.country.fl_str_mv Brasil
dc.publisher.department.fl_str_mv Química
publisher.none.fl_str_mv Universidade Federal de Santa Maria
Centro de Ciências Naturais e Exatas
dc.source.none.fl_str_mv reponame:Biblioteca Digital de Teses e Dissertações do UFSM
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collection Biblioteca Digital de Teses e Dissertações do UFSM
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