Desenvolvimento, fabricação e aplicação de dispositivos eletroquímicos usando impressão 3D

• Ano de defesa: 2020
• Autor(a) principal: Cardoso, Rafael Melo
• Orientador(a): Muñoz, Rodrigo Alejandro Abarza
• Banca de defesa: Silva, Iranaldo Santos da , Petruci, João Flávio da Silveira , Felix, Fabiana da Silva , Sgobbi, Lívia Flório
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: eng
• Instituição de defesa: Universidade Federal de Uberlândia
• Programa de Pós-Graduação: Programa de Pós-graduação em Química
• País: Brasil
• Palavras-chave em Português: 3d-printing; Additive manufacturing; Electrochemical sensors; Bioanalysis; Forensics; Impressão 3D; Sensores eletroquímicos; Forense; Bioanálise; Ácido polilático(PLA)
• Área do conhecimento CNPq: CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA::QUIMICA ANALITICA::ELETROANALITICA
• Link de acesso: https://repositorio.ufu.br/handle/123456789/30327; http://doi.org/10.14393/ufu.te.2020.723

Recent advances in the manufacturing of electroanalytical sensors, cells and devices using 3D-printing is the focus of this work. This thesis introduces this theme/topic with a wide and critical literature review, followed by several proposed applications at electroanalytical prototyping and sensing. The first of those is an electroanalytical cell, for hydrodynamic and stationary measurements, and 3D-printed sensors based on a conductive thermoplastic with carbonaceous materials (graphene or carbon black). The electrodes were applied in the forensic field by quantification, detection and sampling 2,4,6-trinitrotoluene, well known as TNT. The proposed device is a flexible samplersensor for suspect powders in crime scenes and presented proper analytical characteristics reaching a LOD of 0.4 mol L−1 in a linear interval of 1 – 870 molL−1 for TNT. This thesis also shows how a 3D pen can be used to fabricate electrochemical sensors, also proposed for TNT detection,presenting higher LOD, but interesting characteristics such as low volume in a drop of 100 L, low conductive plastic consumption and voltammetric results similar to a commercial SPE. All this in portable shape cylindrical or three in one electrode. In the field of bioanalytics, glucose was a target molecule for 3D-printed electrodes modified with glucose oxidase, using chronoamperometry, reaching LOD of 15 molL−1, inter-day and intra-day precision lower than 5 %, and adequate recovery values (90–105 %) for the analysis of blood plasma. A simultaneous method using amperometric detection of nitrite and uric acid within a linear range from 0.5–250 mol L−1 for both analytes, LODs of 0.02 and 0.03 mol L−1 for uric acid and nitrite, respectively, and high precision (RSD < 2.1 % were obtained). This thesis also shows the first application of 3D-printed sensors and biosensors for the analysis of real biological samples with analytical features comparable to conventional modified electrodes.All the 3D-printed devices presented a unit cost lower than U$0.50 and high precision of fabrication (RSD = 4%).