Avaliação de biomarcadores salivares de estresse oxidativo após meia maratona em corredores amadores

• Ano de defesa: 2013
• Autor(a) principal: Santos, Erickson Messias Bezerra dos
• Orientador(a): Botelho, Françoise Vasconcelos
• Banca de defesa: Silva, Nilson Penha , Pinto, Verônica Salerno
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Uberlândia
• Programa de Pós-Graduação: Programa de Pós-graduação em Genética e Bioquímica
• Departamento: Ciências Biológicas
• País: BR
• Palavras-chave em Português: Meia maratona; Saliva; Estresse oxidativo; Bioquímica
• Palavras-chave em Inglês: Half marathon; Oxidative stress
• Área do conhecimento CNPq: CNPQ::CIENCIAS BIOLOGICAS::GENETICA
• Link de acesso: https://repositorio.ufu.br/handle/123456789/15873

CHAPTER 2: Exercise is considered a promoter of reactive oxygen and nitrogen (RONS), the magnitude of this process is dependent on the type, duration and intensity of exercise. The emergence of analyzes and sample collection faster and easier, such as saliva collection, offers a promising future for the analyzes to be applied in the field to quantify the production of these species to the exercise. The aim of this study was to evaluate and analyze some markers of the redox balance, using the saliva of young and active before, immediately after and 24 hours after competing in a half marathon. The sample consisted of 11 individuals five men and six women (23.93 ± 4.9 years, 1.69 ± 0.07 cm, 62.80 ± 6.6 kg). The volunteers completed a proof of street racing with a route of 21.1 km. Our findings show that in the studied group there were no changes in the rates of oxidative damage to lipids before (0, 574 ± 0, 068 nmol TBARS / mg protein) after (0.407 ± 0.072 nmol TBARS / mg protein) and 24 hours after the test (TBARS 0.154 ± 0.060 nmol / mg protein) that were measured by the concentration of lipid peroxidation products (TBARS). On the other hand antioxidant response was increased after the test (305.5 ± 118.8 μmol Trolox eq / L) and 24 hours (369.4 ± 219.2 μmol Trolox eq / L), shown by total antioxidant capacity ( FRAP). The concentration of GSH was not different after the race (4.503 ± 0.387 nM / mg protein), but had decreased 24 hours after the test (3.356 ± 0.477 nM / mg protein). Catalase activity did not change significantly in any of the time points evaluated (5,686 ± 4,645 vs. 1,863 ± 6,581 before after vs. 3,449 ± 1,826 U / mg protein after 24 hours). These results suggest that for this group, even a long duration exercise as a half marathon, was not sufficient to cause oxidative damage to lipids since the answers antioxidants increased after the race.