Hidroxiapatita, policaprolactona e alendronato em defeitos ósseos experimentais no olecrano de coelhos

• Ano de defesa: 2013
• Autor(a) principal: Reyes Ochoa, Clara Cecilia
• Orientador(a): Borges, Andrea Pacheco Batista
• Banca de defesa: Bicalho, Sheila Maria de Castro Máximo , Reis, Emily Correna Carlo , Valente, Fabrício Luciani , Costa Neto, João Moreira da
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Viçosa
• Programa de Pós-Graduação: Mestrado em Medicina Veterinária
• Departamento: Biotecnologia, diagnóstico e controle de doenças; Epidemiologia e controle de qualidade de prod. de
• País: BR
• Palavras-chave em Português: Regeneração óssea; Biomaterial; Biocompatibilidade
• Palavras-chave em Inglês: Bone regeneration; Biomaterial; Biocompatibility
• Área do conhecimento CNPq: CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA
• Link de acesso: http://locus.ufv.br/handle/123456789/5155

Bone loss is a problem, both in medicine and in veterinary medicine, which has led to researchers in the search for biomaterials to promote bone regeneration. This study aimed to evaluate the biocompatibility, osseointegration and biodegradation of the composites of hydroxyapatite (HA), polycaprolactone (PCL) and alendronate (ALO) and the polymer polycaprolactone (PCL) in circular bone defects of 5 mm diameter made in the right olecranon of rabbits. The study included 36 New Zealand rabbits that were randomly divided into four groups of equal numbers. The composites were manufactured with different proportions, composite one was composed by 49.5% hydroxyapatite, 49.5% polycaprolactone, 1% alendronate ( PCL + HA + ALO), and composite two was composed by 50% hydroxyapatite, 50% polycaprolactone (PCL + HA) and polymer polycaprolactone (PCL group). Each group received a composition above and one group received no treatment and was used as control. The evaluation was made by clinical examination on days 2, 4 and 8 after surgery and Iameness, pain, infection and dehiscence parameters were evaluated. To assess edema the circumference of the member was measured on days 1, 2, 4 and 8 post-operatively. Radiographs were taken before, immediately after surgery and the day of euthanasia. The dates 8, 45 and 90 postoperative were evaluated, with three animals per group for each date. In the last three dates olecranon was collected for histologic and morphometric analysis. There was no difference in the Iameness, pain, infection and dehiscence parameters between groups. In clinical analysis, differences were observed between the variable edema of the Iimb circumference values on day 1 and 8 in all groups. The X- Rays shown similar radiopaticy 8 days after surgery at the in the PCL+HA+ALO e PCL+HA and a smaller one at the groups PCL e .45 after surgery the groups PCL+HA+ALO e PCL+HA Showed a high percent of similar radiopacity 90 days after surgery the radiopaticy showed was 100% for PCL+HA+ALO, PCL+HA e PCL groups and 83.3% for control group. Histologically, the control group showed edges of the defect easily identifiable beginning of trabecular bone formation, this organized bone, followed by a large number of trabecular bone, the group PCL, after eight days, trabecular bone formation in the vicinity of the defect, since the groups PCL + + ALO HA and PCL + HA formation was observed both in the center and the periphery of the defect. The biomaterials used has direct contact with the tissue proving biocompatibility and also it might occour a bone formation as biomaterials have been degraded, since it was within the bone defect.