Análises citogenéticas e de sequencias específicas de cromossomos B em Partamona cupira (Hymenoptera:Apidae)

• Ano de defesa: 2008
• Autor(a) principal: Marthe, Jefferson de Brito
• Orientador(a): Tavares, Mara Garcia
• Banca de defesa: Campos, Lúcio Antonio de Oliveira , Tosta, Vander Calmon
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Viçosa
• Programa de Pós-Graduação: Mestrado em Genética e Melhoramento
• Departamento: Genética animal; Genética molecular e de microrganismos; Genética quantitativa; Genética vegetal; Me
• País: BR
• Palavras-chave em Português: Hymenoptera; Variabilidade genética; Cromossomo B
• Palavras-chave em Inglês: Hymenoptera; Genetic variability; B chromosome
• Área do conhecimento CNPq: CNPQ::CIENCIAS BIOLOGICAS::GENETICA::GENETICA ANIMAL
• Link de acesso: http://locus.ufv.br/handle/123456789/4690

B chromosomes, called supernumerary or accessory too, has been founded in fungi, plants and animals. This chromosomes have a rate of no mendelian segregation and he can occurs, since one to many copies by individual. In the gender Partamona, of six citogenetic characterazed species nowadays, only P. helleri owned B chromosome. RAPD mark has been identified as associated to this chromosome in a colony from Viçosa City-MG. After this, it has been cloned, sequenciated and transformated in a SCAR mark. This same mark has been identified in P. cupira, P. criptica and P. rustica, what suggested this species have B chromosome, too. Moreover, this same mark has been identified in a colony of Salvador City-BA. whose individuals have a big heterochromatic B chromosome. How it's possible that there is a association between the presence of B chromosomes, this work had as aim detect the presence of B chromosome in P. cupira and verified a possible association between them and the presence of SCAR mark described above. Another object was the sequenciation of SCAR fragment from P. cupira, P. criptica, P. rustica and P. helleri (Salvador City-BA), with the aim of comparer the level of identity between the SCAR sequences. As resulted, the four colony at all from P. cupira analised, only two ownied the chromosomic number 2n=34, otherwise the other two colonies ownied same individuals that demonstrated have the presence of a great B chromosome (GUI 1 E GUI 11). Another observation was CMA3 mark in the short arm of this supernumerary chromosomes, what suggest that them may have owned ribossomal DNA sequeces. Moreover, it has been verified a clear association between the presence of SCAR mark and B chromosomes in the individuals from colonies GUI 1 E GUI 11. It's considered that sequences compared at all, have been only same gaps and almost none variable site, between them, what suggests that the level of identity between sequences is extremely high. It's suggests some kind of importance in relation to the adaptation of chromosome B, nowadays unknown, or the born of B, throw interspecific mating no detected yet. Future studies will analyze the different hypothesis about the origin of B chromosomes in the genera Partamona and the association between fragments from SCAR primers with the presence of Bs in P. criptica, P. rustica e P. helleri (Salvador City-BA).