Identificação dos genes eIF4E e eIF(iso)4E em Solanum habrochaites f. glabratum e introgressão do alelo de resistência ao PepYMV(Pepper yellow mosaic virus)em tomateiro

• Ano de defesa: 2012
• Autor(a) principal: Soares, Marcelo Oliveira
• Orientador(a): Silva, Derly José Henriques da
• Banca de defesa: Gomes, Carlos Nick , Souza, Moacil Alves de , Urquiza, Gloria Patricia Castillo
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Viçosa
• Programa de Pós-Graduação: Doutorado em Genética e Melhoramento
• Departamento: Genética animal; Genética molecular e de microrganismos; Genética quantitativa; Genética vegetal; Me
• País: BR
• Palavras-chave em Português: Potyvírus Pepper yellow mosaic virus; Produção de tomates; Retrocruzamento; Recursos genéticos
• Palavras-chave em Inglês: Potyvirus Pepper yellow mosaic virus; Production of tomatoes; Backcross; genetic resources
• Área do conhecimento CNPq: CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA::MELHORAMENTO VEGETAL
• Link de acesso: http://locus.ufv.br/handle/123456789/1355

The incidence of the potyvirus Pepper yellow mosaic virus is a major problem in tomato production in Brazil, requiring the planting of resistant cultivars in order to minimize losses related to this biotic stress. The use of resistance genes present in access stored in genebank is essential for obtaining resistant cultivars. Through studies, and can identify natural mutations in the factor of eukaryotic translation initiation eIF4E and eIF(iso)4E carrying recessive resistance to the virus through the reduction or even absence of viral replication. Our objectives were (i) to identify the alleles eIF4E and eIF(iso)4E access found in wild tomato (Solanum habrochaites f. Glabratum) BGH6902 PepYMV resistant and susceptible cultivar Santa Clara, and (ii) transfer the allele that confers resistance to strains of tomato. For this, cDNAs corresponding to the alleles of eIF4E and eIF(iso)4E access BGH6902 and Santa Clara were cloned and sequenced, along with the realization of backcrossing and selection of progenies from this cross contrasting genetic resistance. Progeny were selected with a low virus concentration, such as RC2-123-4 and RC2-98-2 was identified in which high level of impact resistance of PepYMV. In comparing the amino acid sequences of eIF4E and eIF(iso)4E, both amplified and BGH6902 Santa Clara, were given four non-synonymous substitutions for eIF4E (P69S, V85L, K123Q, and N224S) and four for eIF(iso)4e (V46A , H56Y, V78L and V175I).