Avaliação do uso do ácido linoléico conjugado e da vitamina E na progressão da aterosclerose em camundongos knockout para o receptor de LDL

• Ano de defesa: 2007
• Autor(a) principal: Rezende, Angélica Heringer de
• Orientador(a): Sabarense, Céphora Maria
• Banca de defesa: Gloria, Maria Beatriz Abreu , Neves, Clóvis Andrade
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Viçosa
• Programa de Pós-Graduação: Mestrado em Ciência da Nutrição
• Departamento: Valor nutricional de alimentos e de dietas; Nutrição nas enfermidades agudas e crônicas não transmis
• País: BR
• Palavras-chave em Português: Ácidos graxos; Lipídios; Vitamina E; Aterosclerose; Tratamento; Aspectos nutricionais; Camundongo
• Palavras-chave em Inglês: Fatty acids; Lipids; Vitamin E; Atherosclerosis; Treatment; Nutritional aspects; Mice
• Área do conhecimento CNPq: CNPQ::CIENCIAS DA SAUDE::NUTRICAO
• Link de acesso: http://locus.ufv.br/handle/123456789/2813

This study aimed to evaluate the effects of CLA and/or vitamin E supplementation in the progression of atherosclerosis and in the fatty acids profile of tissues of LDLR -/- mice, a model for familial hypercholesterolemia. The animals were fed with a semi purified diet (CT) or with an atherogenic diet (AT), during six weeks. After this period, in order to evaluating the atherosclerosis development degree, a group of animals was euthanized. The other animals were fed during eight weeks later with a semi purified diet supplemented with 1% of vitamin E (TOC), 1% of 10t,12c- CLA (CLA) or with 1% of vitamin E + 1% of 10t,12c-CLA (CLA/TOC). The weight and the diet consumption were monitored weekly and daily, respectively. Fatty acid serum profile was determined, as well as liver and feces concentrations of α-tocopherol and cholesterol. Peroxidation in liver was evaluated by hydroperoxides analysis. The fatty acids profiles of the liver, adipose tissue and small intestine were analyzed by gas chromatography. The total area of the atherosclerotic lesion and the aorta obstruction percentile were estimated. Serum lipids profile showed total cholesterol, LDL and triacylglycerols smaller concentrations in the supplemented with vitamin E groups. In these groups the cholesterol excretion and the hepatic α-tocopherol concentrations were larger than in others. However, in the group that received with vitamin E only, was found the biggest degree of hepatic lipids peroxidation. The fatty acids profiles of tissues reflected the dietary fatty acids composition. Althought, in none tissues analyzed was possible to identify the incorporation of 10t,12c-CLA. Probably the CLA was not detected due to its rapidly metabolization. In all of the experimental groups, the polyunsaturated fatty acids percentage in the adipose tissue and intestine was larger than in CT group. The use of 10t,12c-CLA isomer individually did not impede the atherosclerotic lesions development in the aorta. On the other hand, the dietary supplementation with vitamin E, as well as the vitamin E and 10t,12c-CLA association significantly reduced the total area of the atherosclerotic lesion and the aorta obstruction percentile. Showing the better action of vitamin E than 10t,12c-CLA in reduce the atherosclerosis progression, despite their antioxidant effect.