Atividade hepatoprotetora do extrato da casca de Bathysa cuspidata (A. St. Hil.) Hook. f. contra estresse oxidativo induzido pelo tetracloreto de carbono em ratos Wistar

• Ano de defesa: 2010
• Autor(a) principal: Gonçalves, Reggiani Vilela
• Orientador(a): Matta, Sérgio Luis Pinto da
• Banca de defesa: Peters, Vera Maria , Peluzio, Maria do Carmo Gouveia
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Viçosa
• Programa de Pós-Graduação: Doutorado em Biologia Celular e Estrutural
• Departamento: Análises quantitativas e moleculares do Genoma; Biologia das células e dos tecidos
• País: BR
• Palavras-chave em Português: Toxicologia; Estresse oxidativo; Bathaysa cuspidata
• Palavras-chave em Inglês: Toxicology; Oxidative stress; Bathaysa cuspidata
• Área do conhecimento CNPq: CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
• Link de acesso: http://locus.ufv.br/handle/123456789/280

Bathysa cuspidata belongs to the Rubiaceae family and is used in popular medicine for the treatment of stomach and liver disorders and as a healing agent. The objective this study was investigate the effect of stem bark ethanolic extract of Bathysa cuspidata (A. St. Hil.) Hook. f. on liver lesions induced by carbon tetrachloride (CCI 4) in Wistar rats. Liver lesions were induced by intraperitonial administration of CCI4 every 48 hours for 12 days. The animals were treated with B. cuspidata extract (BCE) administered by gavage. Seventy rats were randomized into 10 treatment groups with 7 animals in each group, to achieve the curative and Preventive study, being made up as follows: Curative groups: Group 1: CC!4 + 12 days; Group 2: CCI4; Group 3: CCI4 + dimethyl sulfoxide; Group 4: BCE 400 mg/kg; Group 5: CCI4 + BCE 200 mg/kg; Group 6: CCI4 + BCE 400 mg/kg. Preventive groups: Group 1 (G1): CCl4 without BCE; Group 2 (G2): DMSO + CCl4 (dimethyl sulfoxide 700 &#956;l); Group 3 (G3): BCE (BCE 400 mg/kg alone for 18 days); Group 4 (G4): BCE 200 mg/kg + CCl4; Group 5 (G5): BCE 400 mg/kg + CCl4. In all the animals, treatment with the extract was initiated six days prior to the first application of CCl4. Curative: Final weight and hepatosomatic index were significantly reduced in Group 2 compared to the other groups. Animals in Group 2 also had the highest serum ALT and AST levels. BCE administration resulted in a significant reduction in the quantity of hydroperoxides and malondialdehyde compared to the other groups. The number of lipid droplets in the liver was also lower in the BCE groups. A significant increase was found in superoxide dismutase activity and catalase enzymes in groups 5 and 6 compared to the other groups. Preventive: A significant reduction (p<0.05) in serum aspartate transaminase (AST) levels and a significant increase (p<0.05) in the antioxidant enzymes super oxide dismutase (SOD) and catalase (CAT) were found in the livers of the animals that received the extract. Extensive areas of necrosis and lipid droplets were found in the livers of the animals in G1 and G2. Conversely, a significant reduction (p<0.05) was found in the areas of lipid droplets and cell necrosis in the liver tissue of the animals that received the extract. The results of both studies demonstrate that the bark extract of B. cuspidata stimulates antioxidant defense system and reduces the morphological and functional lesions in the liver of rats exposed to CCl4.