Análise proteômica de raízes de soja em resposta à inoculação com nematoides de galha

• Ano de defesa: 2012
• Autor(a) principal: Santana, Fernanda Abreu
• Orientador(a): Moreira, Maurílio Alves
• Banca de defesa: Fietto, Luciano Gomes , Oliveira, Márcia Rodrigues Carvalho
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Viçosa
• Programa de Pós-Graduação: Doutorado em Genética e Melhoramento
• Departamento: Genética animal; Genética molecular e de microrganismos; Genética quantitativa; Genética vegetal; Me
• País: BR
• Palavras-chave em Português: Proteína; Resistência; Interação planta-patógeno
• Palavras-chave em Inglês: Protein; Resistance; Plant-pathogen interactions
• Área do conhecimento CNPq: CNPQ::CIENCIAS AGRARIAS::RECURSOS FLORESTAIS E ENGENHARIA FLORESTAL::SILVICULTURA::GENETICA E MELHORAMENTO FLORESTAL
• Link de acesso: http://locus.ufv.br/handle/123456789/1358

The expansion of soybean cultivation in Brazil has been accompanied by increases in occurrence of diseases and pests of this species, with these factors now limiting the obtainment of higher crop yields. Amongst the pathogens that attack soybean root knot nematodes are notable for occurring throughout the country and causing serious crop damage. Studies aiming to improve our understanding of plant-nematode interactions have been carried out but there remains a lack of information in this area. The analysis of proteins by 2D-electrophoresis associated with mass spectrometry is a well established and refined technique that can be used to investigate the molecular aspects of plant-pathogen interactions. The objective of this work was therefore to identify proteins in soybean roots that are differentially expressed in response to inoculation with root knot nematodes (Meloidogyne javanica and Meloidogyne incognita). Samples of roots from the resistant Conquista variety were evaluated which had been collected 12, 24 and 48 hours after inoculation and roots from non-inoculated plants were used as a control. Proteins were extracted using the phenol-SDS method and separated by 2D electrophoresis. Differentially expressed proteins were then detected by comparative analysis of the gels and identified using mass spectrometry (MS/MS). Various proteins/enzymes associated with cell signalling (annexin), metabolism of reactive oxygen species (ascorbate peroxidase, glutathione transferase), production of the plant hormones ethylene and jasmonic acid (protein related to maturation, the precursor of the 31kDa glycoprotein stem), lignin biosynthesis (Caffeoyl-CoA methyltransferase), amino acid metabolism (glutamine synthase, glutamate dehydrogenase, methionine synthase), redox processes (flavoprotein WrbA, isoflavone reductase) and energy metabolism (triose phosphate isomerase, enolase, UGPase, aconitate hydratase, transketolase, 6-phosphogluconate dehydrogenase, phosphoglycerate kinase, phosphoglucomutase, ATP synthase alpha subunit). These results will help to clarify the mechanisms of soybean defence against root knot nematodes and identify candidate genes that may be used in the development of transgenic plants with resistance to nematodes.