Avaliação de fungos nematófagos sobre larvas infectantes de Strongyloides westeri e ovos de Toxocara canis
Ano de defesa: | 2012 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , , , |
Tipo de documento: | Tese |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Viçosa
|
Programa de Pós-Graduação: |
Doutorado em Medicina Veterinária
|
Departamento: |
Biotecnologia, diagnóstico e controle de doenças; Epidemiologia e controle de qualidade de prod. de
|
País: |
BR
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://locus.ufv.br/handle/123456789/1454 |
Resumo: | The objective of this work was to evaluate the action of three isolates of predatory fungi Duddingtonia flagrans (AC001), Monacrosporium thaumasium (NF34) and Arthrobotrys robusta (I-31) in in vitro assay regarding their capacity to prey infective larvae (L3) of Strongyloides westeri and, to evaluate in vitro three concentrations of chlamydospores (1,000, 10,000 and 100,000) of the nematophagous fungus Pochonia chlamydosporia (isolates VC1 and VC4) in the destruction of Toxocara canis eggs. In relation to the fungi predators when compared to the control group, one can observe that there was a significant reduction (P<0.01) of 80.4%, 67.9%, 72.8%, in the means of recovered infective larvae of S. westeri from treatments with the isolates AC001, NF34 and I-31, respectively. All tested isolates were efficient at capturing S. westeri (P>0.01) when tested in vitro. In the test of ovicidal evaluation with the fungus P. chlamydosporia on eggs of T. canis, each Petri dish contained thousand eggs of T. canis with only one concentration of chlamydospores of VC1 or VC4 in water-agar 2% (WA 2%), and only one thousand eggs in WA 2% in the control groups. Within 15 days, one hundred eggs were removed from each Petri dish and the ovicidal effect was evaluated according to morphological alterations. The higher percentage to effect of the type 3 was observed in concentration of 100,000 chlamydospores to both isolates. After in vitro evaluation, predatory and ovicidal fungi were evaluated in vivo regarding their capacity to withstand the passage through the gastrointestinal tract of horses (Duddingtonia flagrans and Monacrosporium thaumasium) on S. westeri and dogs (Pochonia chlamydosporia) on T. canis eggs. The predatory fungi survived the passage through the gastrointestinal tract of horses and were efficient in preying upon the L3 since the first collection (12h) (P <0.01) compared to control (without fungi). The ovicidal fungus also survived the passage through the gastrointestinal tract of dogs keeping its ovicidal activity on T. canis eggs. These results demonstrated that the fungi D. flagrans and M. thaumasium were promising to be used for biological control of S. westeri as well as from the results obtained suggest that the fungus P chlamydosporia (VC4) could be used as a tool for biological control of T. canis eggs. |
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Araujo, Juliana Milanihttp://lattes.cnpq.br/5898960497379985Benjamin, Laércio dos Anjoshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4797917E7Carvalho, Giovanni Ribeiro dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4723068Z6Araújo, Jackson Victor dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780836D6Lima, Eraldo Rodrigues dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783762J5Braga, Fábio Ribeirohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4537780H4Negrão-correa, Deborah Aparecidahttp://lattes.cnpq.br/3655645192101507Lima, Walter dos Santoshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783038T62015-03-26T12:47:47Z2013-05-142015-03-26T12:47:47Z2012-07-17ARAUJO, Juliana Milani. Evaluation of nematophagous fungi on infective larvae of the Strongyloides westeri and Toxocara canis eggs. 2012. 78 f. Tese (Doutorado em Biotecnologia, diagnóstico e controle de doenças; Epidemiologia e controle de qualidade de prod. de) - Universidade Federal de Viçosa, Viçosa, 2012.http://locus.ufv.br/handle/123456789/1454The objective of this work was to evaluate the action of three isolates of predatory fungi Duddingtonia flagrans (AC001), Monacrosporium thaumasium (NF34) and Arthrobotrys robusta (I-31) in in vitro assay regarding their capacity to prey infective larvae (L3) of Strongyloides westeri and, to evaluate in vitro three concentrations of chlamydospores (1,000, 10,000 and 100,000) of the nematophagous fungus Pochonia chlamydosporia (isolates VC1 and VC4) in the destruction of Toxocara canis eggs. In relation to the fungi predators when compared to the control group, one can observe that there was a significant reduction (P<0.01) of 80.4%, 67.9%, 72.8%, in the means of recovered infective larvae of S. westeri from treatments with the isolates AC001, NF34 and I-31, respectively. All tested isolates were efficient at capturing S. westeri (P>0.01) when tested in vitro. In the test of ovicidal evaluation with the fungus P. chlamydosporia on eggs of T. canis, each Petri dish contained thousand eggs of T. canis with only one concentration of chlamydospores of VC1 or VC4 in water-agar 2% (WA 2%), and only one thousand eggs in WA 2% in the control groups. Within 15 days, one hundred eggs were removed from each Petri dish and the ovicidal effect was evaluated according to morphological alterations. The higher percentage to effect of the type 3 was observed in concentration of 100,000 chlamydospores to both isolates. After in vitro evaluation, predatory and ovicidal fungi were evaluated in vivo regarding their capacity to withstand the passage through the gastrointestinal tract of horses (Duddingtonia flagrans and Monacrosporium thaumasium) on S. westeri and dogs (Pochonia chlamydosporia) on T. canis eggs. The predatory fungi survived the passage through the gastrointestinal tract of horses and were efficient in preying upon the L3 since the first collection (12h) (P <0.01) compared to control (without fungi). The ovicidal fungus also survived the passage through the gastrointestinal tract of dogs keeping its ovicidal activity on T. canis eggs. These results demonstrated that the fungi D. flagrans and M. thaumasium were promising to be used for biological control of S. westeri as well as from the results obtained suggest that the fungus P chlamydosporia (VC4) could be used as a tool for biological control of T. canis eggs.O objetivo deste trabalho foi avaliar a ação de três isolados de fungos predadores Duddingtonia flagrans (AC001), Monacrosporium thaumasium (NF34) e Arthrobotrys robusta (I-31) em teste in vitro quanto à capacidade de predar larvas infectantes (L3) de Strongyloides westeri e, avaliar três concentrações de clamidósporos (1.000, 10.000 e 100.000) do fungo ovicida Pochonia chlamydosporia (isolados VC1 e VC4) na destruição de ovos de Toxocara canis. Em relação aos fungos predadores, quando comparado ao grupo controle, pode-se observar que houve uma redução significativa (P<0,01) de 80,4%, 67,9%, 72,8%, nas médias de larvas infectantes de S. westeri recuperadas dos tratamentos com os isolados AC001, NF34 e I-31, respectivamente. Todos os isolados testados foram eficientes na captura de S. westeri (P>0,01) no teste in vitro. No teste da avaliação ovicida com o fungo P. chlamydosporia sobre ovos de T. canis, cada placa de Petri continha mil ovos de T. canis com apenas uma das concentrações de clamidósporos de VC1 ou de VC4, em ágar-água 2% (AA 2%) e, mil ovos em AA 2% nos grupos controle. No intervalo de 15 dias, cem ovos foram retirados de cada placa de Petri e a atividade ovicida foi avaliada quanto as alterações morfológicas. A maior destruição dos ovos (efeito do tipo 3) foi observada na concentração de 100.000 clamidósporos para ambos os isolados. Após avaliação in vitro, os fungos predadores e ovicida, foram avaliados in vivo, quanto à sua capacidade de suportar a passagem pelo trato gastrintestinal de eqüídeos (AC001 e NF34) e predar L3 de S. westeri, e pelo trato gastrintestinal cães (VC4) quanto sua ação ovicida sobre ovos de T. canis. Os fungos predadores sobreviveram à passagem pelo trato gastrintestinal dos eqüídeos e foram eficientes em predar as L3 de S. westeri desde a primeira coleta (12h) (P<0,01) em relação ao grupo controle (sem fungo). O fungo ovicida também sobreviveu a passagem pelo trato gastrintestinal de cães mantendo sua atividade ovicida sobre os ovos de T. canis. Esses resultados demonstraram que os fungos D. flagrans e M. thaumasium se mostraram promissores para serem utilizados no controle biológico de S. westeri assim como, resultados obtidos sugere-se que o fungo P. chlamydosporia (VC4) poderia ser utilizado como uma ferramenta no controle biológico de ovos de T. canis.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de ViçosaDoutorado em Medicina VeterináriaUFVBRBiotecnologia, diagnóstico e controle de doenças; Epidemiologia e controle de qualidade de prod. deFungos nematófagosToxocara canisCãesNematophagous fungiToxocara canisDogsCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIAAvaliação de fungos nematófagos sobre larvas infectantes de Strongyloides westeri e ovos de Toxocara canisEvaluation of nematophagous fungi on infective larvae of the Strongyloides westeri and Toxocara canis eggsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf924224https://locus.ufv.br//bitstream/123456789/1454/1/texto%20completo.pdf81650fc2d857cec2b95911395c33c4b9MD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain118246https://locus.ufv.br//bitstream/123456789/1454/2/texto%20completo.pdf.txt1bfaff085eac5fc2c431391ef32d98a7MD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3602https://locus.ufv.br//bitstream/123456789/1454/3/texto%20completo.pdf.jpg6a4a6ceb3944cb7cae1fd472488f12e4MD53123456789/14542016-04-07 23:04:38.491oai:locus.ufv.br:123456789/1454Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-08T02:04:38LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.por.fl_str_mv |
Avaliação de fungos nematófagos sobre larvas infectantes de Strongyloides westeri e ovos de Toxocara canis |
dc.title.alternative.eng.fl_str_mv |
Evaluation of nematophagous fungi on infective larvae of the Strongyloides westeri and Toxocara canis eggs |
title |
Avaliação de fungos nematófagos sobre larvas infectantes de Strongyloides westeri e ovos de Toxocara canis |
spellingShingle |
Avaliação de fungos nematófagos sobre larvas infectantes de Strongyloides westeri e ovos de Toxocara canis Araujo, Juliana Milani Fungos nematófagos Toxocara canis Cães Nematophagous fungi Toxocara canis Dogs CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Avaliação de fungos nematófagos sobre larvas infectantes de Strongyloides westeri e ovos de Toxocara canis |
title_full |
Avaliação de fungos nematófagos sobre larvas infectantes de Strongyloides westeri e ovos de Toxocara canis |
title_fullStr |
Avaliação de fungos nematófagos sobre larvas infectantes de Strongyloides westeri e ovos de Toxocara canis |
title_full_unstemmed |
Avaliação de fungos nematófagos sobre larvas infectantes de Strongyloides westeri e ovos de Toxocara canis |
title_sort |
Avaliação de fungos nematófagos sobre larvas infectantes de Strongyloides westeri e ovos de Toxocara canis |
author |
Araujo, Juliana Milani |
author_facet |
Araujo, Juliana Milani |
author_role |
author |
dc.contributor.authorLattes.por.fl_str_mv |
http://lattes.cnpq.br/5898960497379985 |
dc.contributor.author.fl_str_mv |
Araujo, Juliana Milani |
dc.contributor.advisor-co1.fl_str_mv |
Benjamin, Laércio dos Anjos |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4797917E7 |
dc.contributor.advisor-co2.fl_str_mv |
Carvalho, Giovanni Ribeiro de |
dc.contributor.advisor-co2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4723068Z6 |
dc.contributor.advisor1.fl_str_mv |
Araújo, Jackson Victor de |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780836D6 |
dc.contributor.referee1.fl_str_mv |
Lima, Eraldo Rodrigues de |
dc.contributor.referee1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783762J5 |
dc.contributor.referee2.fl_str_mv |
Braga, Fábio Ribeiro |
dc.contributor.referee2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4537780H4 |
dc.contributor.referee3.fl_str_mv |
Negrão-correa, Deborah Aparecida |
dc.contributor.referee3Lattes.fl_str_mv |
http://lattes.cnpq.br/3655645192101507 |
dc.contributor.referee4.fl_str_mv |
Lima, Walter dos Santos |
dc.contributor.referee4Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783038T6 |
contributor_str_mv |
Benjamin, Laércio dos Anjos Carvalho, Giovanni Ribeiro de Araújo, Jackson Victor de Lima, Eraldo Rodrigues de Braga, Fábio Ribeiro Negrão-correa, Deborah Aparecida Lima, Walter dos Santos |
dc.subject.por.fl_str_mv |
Fungos nematófagos Toxocara canis Cães |
topic |
Fungos nematófagos Toxocara canis Cães Nematophagous fungi Toxocara canis Dogs CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
dc.subject.eng.fl_str_mv |
Nematophagous fungi Toxocara canis Dogs |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
The objective of this work was to evaluate the action of three isolates of predatory fungi Duddingtonia flagrans (AC001), Monacrosporium thaumasium (NF34) and Arthrobotrys robusta (I-31) in in vitro assay regarding their capacity to prey infective larvae (L3) of Strongyloides westeri and, to evaluate in vitro three concentrations of chlamydospores (1,000, 10,000 and 100,000) of the nematophagous fungus Pochonia chlamydosporia (isolates VC1 and VC4) in the destruction of Toxocara canis eggs. In relation to the fungi predators when compared to the control group, one can observe that there was a significant reduction (P<0.01) of 80.4%, 67.9%, 72.8%, in the means of recovered infective larvae of S. westeri from treatments with the isolates AC001, NF34 and I-31, respectively. All tested isolates were efficient at capturing S. westeri (P>0.01) when tested in vitro. In the test of ovicidal evaluation with the fungus P. chlamydosporia on eggs of T. canis, each Petri dish contained thousand eggs of T. canis with only one concentration of chlamydospores of VC1 or VC4 in water-agar 2% (WA 2%), and only one thousand eggs in WA 2% in the control groups. Within 15 days, one hundred eggs were removed from each Petri dish and the ovicidal effect was evaluated according to morphological alterations. The higher percentage to effect of the type 3 was observed in concentration of 100,000 chlamydospores to both isolates. After in vitro evaluation, predatory and ovicidal fungi were evaluated in vivo regarding their capacity to withstand the passage through the gastrointestinal tract of horses (Duddingtonia flagrans and Monacrosporium thaumasium) on S. westeri and dogs (Pochonia chlamydosporia) on T. canis eggs. The predatory fungi survived the passage through the gastrointestinal tract of horses and were efficient in preying upon the L3 since the first collection (12h) (P <0.01) compared to control (without fungi). The ovicidal fungus also survived the passage through the gastrointestinal tract of dogs keeping its ovicidal activity on T. canis eggs. These results demonstrated that the fungi D. flagrans and M. thaumasium were promising to be used for biological control of S. westeri as well as from the results obtained suggest that the fungus P chlamydosporia (VC4) could be used as a tool for biological control of T. canis eggs. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-07-17 |
dc.date.available.fl_str_mv |
2013-05-14 2015-03-26T12:47:47Z |
dc.date.accessioned.fl_str_mv |
2015-03-26T12:47:47Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
ARAUJO, Juliana Milani. Evaluation of nematophagous fungi on infective larvae of the Strongyloides westeri and Toxocara canis eggs. 2012. 78 f. Tese (Doutorado em Biotecnologia, diagnóstico e controle de doenças; Epidemiologia e controle de qualidade de prod. de) - Universidade Federal de Viçosa, Viçosa, 2012. |
dc.identifier.uri.fl_str_mv |
http://locus.ufv.br/handle/123456789/1454 |
identifier_str_mv |
ARAUJO, Juliana Milani. Evaluation of nematophagous fungi on infective larvae of the Strongyloides westeri and Toxocara canis eggs. 2012. 78 f. Tese (Doutorado em Biotecnologia, diagnóstico e controle de doenças; Epidemiologia e controle de qualidade de prod. de) - Universidade Federal de Viçosa, Viçosa, 2012. |
url |
http://locus.ufv.br/handle/123456789/1454 |
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Universidade Federal de Viçosa |
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Doutorado em Medicina Veterinária |
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UFV |
dc.publisher.country.fl_str_mv |
BR |
dc.publisher.department.fl_str_mv |
Biotecnologia, diagnóstico e controle de doenças; Epidemiologia e controle de qualidade de prod. de |
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Universidade Federal de Viçosa |
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