Avaliação de Listeria monocytogenes em melão e jabuticaba

• Ano de defesa: 2014
• Autor(a) principal: Souza, Poliana Bergamin Athayde de
• Orientador(a): Andrade, Nélio José de
• Banca de defesa: Antunes, Maria Aparecida
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Viçosa
• Programa de Pós-Graduação: Mestrado em Ciência e Tecnologia de Alimentos
• Departamento: Ciência de Alimentos; Tecnologia de Alimentos; Engenharia de Alimentos
• País: BR
• Palavras-chave em Português: Alimentos frescos; Listeriose; Frutas
• Palavras-chave em Inglês: Fresh food; Listeriosis; Fruits
• Área do conhecimento CNPq: CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS::CIENCIA DE ALIMENTOS
• Link de acesso: http://locus.ufv.br/handle/123456789/2949

Consumer demand for fresh foods has grown substantially in recent decades. However, despite these products preserve better sensory and nutritional characteristics, contamination by pathogens, such as L. monocytogenes, generates increasing concerns.Infection by this bacteria, called listeriosis, has low morbidity but high mortality, and represents a high risk to immunocompromised individuals, pregnant women, elderly and children. Fresh products are considered high risk, since it does not go through steps to ensure the elimination of pathogens, in addition, are normally stored under refrigeration condition in which L. monocytogenes can multiply by providing conditions for growth protrudes in comparison to most bacteria. Given the above, the occurrence of L. monocytogenes was investigated in two fruits commonly consumed in natura: melon and jabuticaba. A total of 150 samples of melon and 60 samples jabuticaba acquired at retail in the municipality of Viçosa-MG, were subjected to laboratory analysis. Through conventional analysis using the method ISO 11290-1, 21 isolates of Listeria spp. were found in the fruits of melon, of which only 14 were confirmed by sequencing the gene. The presence of L. monocytogenes was also evaluated by the mini-VIDAS® LMO and by PCR. A strain of L. monocytogenes was found by PCR, however, this result differed from that obtained by imunoanalítico test, which indicated absence of L. monocytogenes and showed a rate of 3.12% false negative results. Serotyping of strains was performed by the Instituto Oswaldo Cruz (Fiocruz) and the serotyp identified was 1/2b one of the most associated with cases of human listeriosis in the world, suggesting the potential risk of to cause disease. The results evidence that the melon is a potential source of L. monocytogenes and that measures to control and prevent this pathogen should be adopted with greater persistence for products that are usually eaten fresh as fruit.