Influência da somatotropina recombinante bovina (rbST) aplicada em receptoras de embriões bovinos, no dia do estro, sobre variáveis reprodutivas

Detalhes bibliográficos
Ano de defesa: 2006
Autor(a) principal: Marques, Paulo Alexandre Fernandes
Orientador(a): Costa, Eduardo Paulino da lattes
Banca de defesa: Carvalho, Giovanni Ribeiro de lattes, Mâncio, Antonio Bento lattes
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal de Viçosa
Programa de Pós-Graduação: Mestrado em Medicina Veterinária
Departamento: Biotecnologia, diagnóstico e controle de doenças; Epidemiologia e controle de qualidade de prod. de
País: BR
Palavras-chave em Português:
Palavras-chave em Inglês:
Área do conhecimento CNPq:
Link de acesso: http://locus.ufv.br/handle/123456789/5118
Resumo: The objective of the present study was to evaluate the effect of the administration, of 500 mg rbST (recombinant bovine somatotropin) in the moment of estrous of recipient heifers and cows inovulation with fresh embryos and 250 and 500 mg rbST in recipient inovulated with defrosted embryos, in progesterone serum concentration (P4) on the day of the inovulation and at the pregnancy rate at day 30. Blood samples were collected in the day of the inovulation by puncture of the coccygeal vein or artery for assaying of the serum P4 concentration. 259 crossbred recipients, appraised as able for reproduction, were used and divided in two studies. In the first study 144 recipient heifers received fresh embryos, randomly sorted among the experiments. The control (T1) was composed by 77 recipients, and the treated (T2) 67 recipients that received 500mg rbST at the moment of estrous. In a second study, it was used 115 recipients that receive defrosted embryos subdivided in 3 treatments. 53 recipient heifers composed the control (T1), the treated 2 (T2) was composed by 22 recipients that received 250 mg of rbST and the treated 3 (T3) was composed by 40 recipients that received 500 mg of rbST. The pregnancy rate of the first study was 65.7% (44 pregnant recipients) among the animals of T2 and 32.5% (25 pregnant recipients) at T1, which demonstrates the positive effect (P<0.01) from the use of rbST on pregnancy rate. However, the average concentrations of serum P4 in the day of the inovulation was 2.54±0.19 mg/ml for the animals of T1 and 2.27±0.20 mg/ml for the T2, there was no difference (P>0.05). The pregnancy rate of the second study recipients was 45.3% (24 recipients) among the animals of the group T1, 50.0% (11 recipient) for T2 and 52.5% (21 recipient) for T3. The average concentrations of serum P4 in the day of the inovulation were: T1 (control) = 5.41±2.33 mg/ml, T2 (250 mg rbST) = 5.77±2.19 mg/ml and T3 (treated - 500 mg rbST) = 4.77 ± 1.78 mg/ml. The results of the second study demonstrate the absence of effect (P>0.05) on the rbST use over the pregnancy rate and the serum P4 concentration. We can conclude that the administration of 500 mg rbST in recipient inovulation that receive fresh embryo at the moment of the estrous was able to improve the pregnancy rate, but not the concentration of progesterone serum P4. However, when administered 250 or 500 mg rbST in recipient inovulated that receive defrosted embryos, we are able to conclude that there was no positive effect on the pregnancy rate and on the progesterone serum concentration.
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spelling Marques, Paulo Alexandre Fernandeshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4130690Z3Fernandes, Carlos Antônio de Carvalhohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4727345U8Torres, Ciro Alexandre Alveshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787213D4Costa, Eduardo Paulino dahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787237D6Carvalho, Giovanni Ribeiro dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4723068Z6Mâncio, Antonio Bentohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4782731E72015-03-26T13:47:09Z2007-04-172015-03-26T13:47:09Z2006-12-18MARQUES, Paulo Alexandre Fernandes. Influence of recombinant bovine somatotropin (rbST) applied in bovine embryo recipients, at estrous day, upon reproductive variables. 2006. 67 f. Dissertação (Mestrado em Biotecnologia, diagnóstico e controle de doenças; Epidemiologia e controle de qualidade de prod. de) - Universidade Federal de Viçosa, Viçosa, 2006.http://locus.ufv.br/handle/123456789/5118The objective of the present study was to evaluate the effect of the administration, of 500 mg rbST (recombinant bovine somatotropin) in the moment of estrous of recipient heifers and cows inovulation with fresh embryos and 250 and 500 mg rbST in recipient inovulated with defrosted embryos, in progesterone serum concentration (P4) on the day of the inovulation and at the pregnancy rate at day 30. Blood samples were collected in the day of the inovulation by puncture of the coccygeal vein or artery for assaying of the serum P4 concentration. 259 crossbred recipients, appraised as able for reproduction, were used and divided in two studies. In the first study 144 recipient heifers received fresh embryos, randomly sorted among the experiments. The control (T1) was composed by 77 recipients, and the treated (T2) 67 recipients that received 500mg rbST at the moment of estrous. In a second study, it was used 115 recipients that receive defrosted embryos subdivided in 3 treatments. 53 recipient heifers composed the control (T1), the treated 2 (T2) was composed by 22 recipients that received 250 mg of rbST and the treated 3 (T3) was composed by 40 recipients that received 500 mg of rbST. The pregnancy rate of the first study was 65.7% (44 pregnant recipients) among the animals of T2 and 32.5% (25 pregnant recipients) at T1, which demonstrates the positive effect (P<0.01) from the use of rbST on pregnancy rate. However, the average concentrations of serum P4 in the day of the inovulation was 2.54±0.19 mg/ml for the animals of T1 and 2.27±0.20 mg/ml for the T2, there was no difference (P>0.05). The pregnancy rate of the second study recipients was 45.3% (24 recipients) among the animals of the group T1, 50.0% (11 recipient) for T2 and 52.5% (21 recipient) for T3. The average concentrations of serum P4 in the day of the inovulation were: T1 (control) = 5.41±2.33 mg/ml, T2 (250 mg rbST) = 5.77±2.19 mg/ml and T3 (treated - 500 mg rbST) = 4.77 ± 1.78 mg/ml. The results of the second study demonstrate the absence of effect (P>0.05) on the rbST use over the pregnancy rate and the serum P4 concentration. We can conclude that the administration of 500 mg rbST in recipient inovulation that receive fresh embryo at the moment of the estrous was able to improve the pregnancy rate, but not the concentration of progesterone serum P4. However, when administered 250 or 500 mg rbST in recipient inovulated that receive defrosted embryos, we are able to conclude that there was no positive effect on the pregnancy rate and on the progesterone serum concentration.O objetivo do presente estudo foi avaliar o efeito da administração, no dia do estro, de 500 mg de rbST (somatotropina bovina recombinante) em receptoras inovuladas com embriões transferidos à fresco e de 250 e 500 mg de rbST em receptoras inovuladas com embriões descongelados, sobre a concentração sérica de progesterona (P4) no dia da inovulação e a taxa de gestação aos 30 dias. As amostras de sangue foram coletadas no dia das inovulações por punção da veia ou artéria coccígea para análise da concentração sérica de P4. Foram utilizadas 259 receptoras mestiças, avaliadas como aptas a reprodução e divididas em dois estudos. No primeiro estudo foram utilizadas 144 receptoras, inovuladas com embriões à fresco, distribuídas aleatoriamente entre os tratamentos. O controle (T1) foi constituído por 77 receptoras e o tratado (T2) por 67 receptoras que receberam a administração de 500 mg de rbST no momento do estro. Em um segundo estudo utilizou-se 115 receptoras inovuladas com embriões descongelados subdivididas em três tratamentos. O controle (T1) foi constituido por 53 receptoras, o tratado 2 (T2) por 22 receptoras submetidas a administração de 250 mg de rbST e o tratado 3 (T3) por 40 receptoras que receberam a administração de 500 mg de rbST. As fêmeas do primeiro estudo apresentaram taxas de gestações de 65,7% (44 receptoras gestantes) entre os animais do grupo T2 e 32,5% (25 receptoras gestantes) para os do grupo T1, o que demonstra o efeito positivo (P<0,01) do uso da rbST sobre a taxa de gestação. Entretanto, as concentrações médias de P4 sérica no dia da inovulação foram de 2,54±0,19 ng/mL para os animais do T1 e 2,27±0,20 ng/mL para os do T2, não sendo observada diferença (P>0,05). No segundo estudo as taxas de gestações encontradas foram de 45,3% (24 receptoras) para T1, 50% (11 receptoras) para T2 e 52,5% (21 receptoras) para T3. As concentrações médias de P4 sérica no dia da inovulação foram de T1 (controle) = 5,41±2,33 ng/mL, T2 (250 mg rbST) = 5,77±2,19 ng/mL e T3 (500 mg rbST) = 4,77±1,78 ng/mL. Os resultados obtidos neste segundo estudo demonstram a ausência de efeito (P>0,05) do uso da rbST sobre as taxas de gestações e as concentrações séricas de P4. Concluiu-se que a aplicação de 500 mg de rbST em receptoras inovuladas com embriões à fresco no momento do estro, foi capaz de melhorar a taxa de gestação, mas não a concentração sérica de progesterona. No entanto, ao serem administradas 250 ou 500 mg de rbST receptoras inovuladas com embriões descongelados conclui-se a ausência de efeito positivo nas taxas de gestações e nas concentrações séricas de progesterona.Fundação de Amparo a Pesquisa do Estado de Minas Geraisapplication/pdfporUniversidade Federal de ViçosaMestrado em Medicina VeterináriaUFVBRBiotecnologia, diagnóstico e controle de doenças; Epidemiologia e controle de qualidade de prod. deSomatotropina bovinaTaxa de gestaçãoProgesteronaBovine somatotropinPregnancy rateProgesteroneCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::REPRODUCAO ANIMALInfluência da somatotropina recombinante bovina (rbST) aplicada em receptoras de embriões bovinos, no dia do estro, sobre variáveis reprodutivasInfluence of recombinant bovine somatotropin (rbST) applied in bovine embryo recipients, at estrous day, upon reproductive variablesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf269793https://locus.ufv.br//bitstream/123456789/5118/1/texto%20completo.pdf7ace1f379fb4f111e4dbe56c3e1179e0MD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain127668https://locus.ufv.br//bitstream/123456789/5118/2/texto%20completo.pdf.txtc2dca32da50edd7f01d005341893b93bMD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3616https://locus.ufv.br//bitstream/123456789/5118/3/texto%20completo.pdf.jpge04651bb9e36bdea54c5a8b4ba04b42aMD53123456789/51182016-04-11 23:09:22.857oai:locus.ufv.br:123456789/5118Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-12T02:09:22LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.por.fl_str_mv Influência da somatotropina recombinante bovina (rbST) aplicada em receptoras de embriões bovinos, no dia do estro, sobre variáveis reprodutivas
dc.title.alternative.eng.fl_str_mv Influence of recombinant bovine somatotropin (rbST) applied in bovine embryo recipients, at estrous day, upon reproductive variables
title Influência da somatotropina recombinante bovina (rbST) aplicada em receptoras de embriões bovinos, no dia do estro, sobre variáveis reprodutivas
spellingShingle Influência da somatotropina recombinante bovina (rbST) aplicada em receptoras de embriões bovinos, no dia do estro, sobre variáveis reprodutivas
Marques, Paulo Alexandre Fernandes
Somatotropina bovina
Taxa de gestação
Progesterona
Bovine somatotropin
Pregnancy rate
Progesterone
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::REPRODUCAO ANIMAL
title_short Influência da somatotropina recombinante bovina (rbST) aplicada em receptoras de embriões bovinos, no dia do estro, sobre variáveis reprodutivas
title_full Influência da somatotropina recombinante bovina (rbST) aplicada em receptoras de embriões bovinos, no dia do estro, sobre variáveis reprodutivas
title_fullStr Influência da somatotropina recombinante bovina (rbST) aplicada em receptoras de embriões bovinos, no dia do estro, sobre variáveis reprodutivas
title_full_unstemmed Influência da somatotropina recombinante bovina (rbST) aplicada em receptoras de embriões bovinos, no dia do estro, sobre variáveis reprodutivas
title_sort Influência da somatotropina recombinante bovina (rbST) aplicada em receptoras de embriões bovinos, no dia do estro, sobre variáveis reprodutivas
author Marques, Paulo Alexandre Fernandes
author_facet Marques, Paulo Alexandre Fernandes
author_role author
dc.contributor.authorLattes.por.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4130690Z3
dc.contributor.author.fl_str_mv Marques, Paulo Alexandre Fernandes
dc.contributor.advisor-co1.fl_str_mv Fernandes, Carlos Antônio de Carvalho
dc.contributor.advisor-co1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4727345U8
dc.contributor.advisor-co2.fl_str_mv Torres, Ciro Alexandre Alves
dc.contributor.advisor-co2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787213D4
dc.contributor.advisor1.fl_str_mv Costa, Eduardo Paulino da
dc.contributor.advisor1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4787237D6
dc.contributor.referee1.fl_str_mv Carvalho, Giovanni Ribeiro de
dc.contributor.referee1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4723068Z6
dc.contributor.referee2.fl_str_mv Mâncio, Antonio Bento
dc.contributor.referee2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4782731E7
contributor_str_mv Fernandes, Carlos Antônio de Carvalho
Torres, Ciro Alexandre Alves
Costa, Eduardo Paulino da
Carvalho, Giovanni Ribeiro de
Mâncio, Antonio Bento
dc.subject.por.fl_str_mv Somatotropina bovina
Taxa de gestação
Progesterona
topic Somatotropina bovina
Taxa de gestação
Progesterona
Bovine somatotropin
Pregnancy rate
Progesterone
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::REPRODUCAO ANIMAL
dc.subject.eng.fl_str_mv Bovine somatotropin
Pregnancy rate
Progesterone
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::REPRODUCAO ANIMAL
description The objective of the present study was to evaluate the effect of the administration, of 500 mg rbST (recombinant bovine somatotropin) in the moment of estrous of recipient heifers and cows inovulation with fresh embryos and 250 and 500 mg rbST in recipient inovulated with defrosted embryos, in progesterone serum concentration (P4) on the day of the inovulation and at the pregnancy rate at day 30. Blood samples were collected in the day of the inovulation by puncture of the coccygeal vein or artery for assaying of the serum P4 concentration. 259 crossbred recipients, appraised as able for reproduction, were used and divided in two studies. In the first study 144 recipient heifers received fresh embryos, randomly sorted among the experiments. The control (T1) was composed by 77 recipients, and the treated (T2) 67 recipients that received 500mg rbST at the moment of estrous. In a second study, it was used 115 recipients that receive defrosted embryos subdivided in 3 treatments. 53 recipient heifers composed the control (T1), the treated 2 (T2) was composed by 22 recipients that received 250 mg of rbST and the treated 3 (T3) was composed by 40 recipients that received 500 mg of rbST. The pregnancy rate of the first study was 65.7% (44 pregnant recipients) among the animals of T2 and 32.5% (25 pregnant recipients) at T1, which demonstrates the positive effect (P<0.01) from the use of rbST on pregnancy rate. However, the average concentrations of serum P4 in the day of the inovulation was 2.54±0.19 mg/ml for the animals of T1 and 2.27±0.20 mg/ml for the T2, there was no difference (P>0.05). The pregnancy rate of the second study recipients was 45.3% (24 recipients) among the animals of the group T1, 50.0% (11 recipient) for T2 and 52.5% (21 recipient) for T3. The average concentrations of serum P4 in the day of the inovulation were: T1 (control) = 5.41±2.33 mg/ml, T2 (250 mg rbST) = 5.77±2.19 mg/ml and T3 (treated - 500 mg rbST) = 4.77 ± 1.78 mg/ml. The results of the second study demonstrate the absence of effect (P>0.05) on the rbST use over the pregnancy rate and the serum P4 concentration. We can conclude that the administration of 500 mg rbST in recipient inovulation that receive fresh embryo at the moment of the estrous was able to improve the pregnancy rate, but not the concentration of progesterone serum P4. However, when administered 250 or 500 mg rbST in recipient inovulated that receive defrosted embryos, we are able to conclude that there was no positive effect on the pregnancy rate and on the progesterone serum concentration.
publishDate 2006
dc.date.issued.fl_str_mv 2006-12-18
dc.date.available.fl_str_mv 2007-04-17
2015-03-26T13:47:09Z
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dc.identifier.citation.fl_str_mv MARQUES, Paulo Alexandre Fernandes. Influence of recombinant bovine somatotropin (rbST) applied in bovine embryo recipients, at estrous day, upon reproductive variables. 2006. 67 f. Dissertação (Mestrado em Biotecnologia, diagnóstico e controle de doenças; Epidemiologia e controle de qualidade de prod. de) - Universidade Federal de Viçosa, Viçosa, 2006.
dc.identifier.uri.fl_str_mv http://locus.ufv.br/handle/123456789/5118
identifier_str_mv MARQUES, Paulo Alexandre Fernandes. Influence of recombinant bovine somatotropin (rbST) applied in bovine embryo recipients, at estrous day, upon reproductive variables. 2006. 67 f. Dissertação (Mestrado em Biotecnologia, diagnóstico e controle de doenças; Epidemiologia e controle de qualidade de prod. de) - Universidade Federal de Viçosa, Viçosa, 2006.
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