Identificação e validação de marcadores moleculares indel e SNP para lipoxigenases de sementes de soja
Ano de defesa: | 2009 |
---|---|
Autor(a) principal: | |
Orientador(a): | |
Banca de defesa: | , , |
Tipo de documento: | Dissertação |
Tipo de acesso: | Acesso aberto |
Idioma: | por |
Instituição de defesa: |
Universidade Federal de Viçosa
|
Programa de Pós-Graduação: |
Mestrado em Bioquímica Agrícola
|
Departamento: |
Bioquímica e Biologia molecular de plantas; Bioquímica e Biologia molecular animal
|
País: |
BR
|
Palavras-chave em Português: | |
Palavras-chave em Inglês: | |
Área do conhecimento CNPq: | |
Link de acesso: | http://locus.ufv.br/handle/123456789/2411 |
Resumo: | The oxidative degradation of fatty acids from soybean oil fraction, that occurs during the grain processing, develop a flavor of unripe been that modify the palatability and therefore the acceptability of soybean products, resulting in problems for the food industry. The enzyme lipoxygenases (linoleate: O2 oxidoreductase, EC 1.13.11.12), that catalyses this oxidation reaction, are present in soybean seed in the three isozyme forms encoded by three dominant genes, Lox1, Lox2 and Lox3. The selection assisted by molecular markers is an area that has great impact within breeding programs. Recently, a new class of molecular markers, called indel (insertion / deletion) and SNP (Single Nucleotide Polymorphism) have been used. These are based on detection of polymorphisms by the change of a single base in the genome. The identification of molecular markers within the genes encoding lipoxygenase may help in the selection of recessive alleles that determine the absence of lipoxygenase in the seed, accelerating the process of soybean breeding. The objective of this study was verify and identify the polymorphisms in the sequences of genes Lox2 and Lox3, respectively, between varieties of different genetic origin used in the Program of Improvement and quality of Soybean at BIOAGRO/UFV, and tovalidate some of these polymorphisms as molecular markers. A pair of primers for LOX2 and 14 pairs of primers designed to amplify the entire gene LOX3 were used to obtain genes fragments. The sequences were obtained from the direct sequencing of PCR products from each primer pair. Subsequently, a population of RILs contrasting for the presence / absence of seed lipoxygenases, were used for the validation of two SNPs as markers for genes Lox 2 and a indel marker for LOX 3. The genotyping method used was the direct sequencing of fragments that contained the polymorphisms studied. Two SNPs for LOX 2 and the indel contained in the first exon of the gene LOX 3 were co- segregated with the character presence/absence of LOX 2 and 3 respectively in the individuals of the population of RILs. These polymorphisms have the potential to be used as tools in soybean breeding assisted by molecular markers. |
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Silva, Danielle Fernandes dahttp://lattes.cnpq.br/7918289438245309Barros, Everaldo Gonçalves dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781285J6Oliveira, Luiz Orlando dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781626T2Moreira, Maurílio Alveshttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4796105P2Miranda, Fábio Demolinari dehttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4706159T4Schuster, Ivanhttp://lattes.cnpq.br/9991337319211428Moraes, Rita Maria Alves dehttp://lattes.cnpq.br/23969935967812872015-03-26T13:07:27Z2010-02-242015-03-26T13:07:27Z2009-07-06SILVA, Danielle Fernandes da. Identification and validation of indel and SNP molecular markers for soybean seed lipoxygenases. 2009. 78 f. Dissertação (Mestrado em Bioquímica e Biologia molecular de plantas; Bioquímica e Biologia molecular animal) - Universidade Federal de Viçosa, Viçosa, 2009.http://locus.ufv.br/handle/123456789/2411The oxidative degradation of fatty acids from soybean oil fraction, that occurs during the grain processing, develop a flavor of unripe been that modify the palatability and therefore the acceptability of soybean products, resulting in problems for the food industry. The enzyme lipoxygenases (linoleate: O2 oxidoreductase, EC 1.13.11.12), that catalyses this oxidation reaction, are present in soybean seed in the three isozyme forms encoded by three dominant genes, Lox1, Lox2 and Lox3. The selection assisted by molecular markers is an area that has great impact within breeding programs. Recently, a new class of molecular markers, called indel (insertion / deletion) and SNP (Single Nucleotide Polymorphism) have been used. These are based on detection of polymorphisms by the change of a single base in the genome. The identification of molecular markers within the genes encoding lipoxygenase may help in the selection of recessive alleles that determine the absence of lipoxygenase in the seed, accelerating the process of soybean breeding. The objective of this study was verify and identify the polymorphisms in the sequences of genes Lox2 and Lox3, respectively, between varieties of different genetic origin used in the Program of Improvement and quality of Soybean at BIOAGRO/UFV, and tovalidate some of these polymorphisms as molecular markers. A pair of primers for LOX2 and 14 pairs of primers designed to amplify the entire gene LOX3 were used to obtain genes fragments. The sequences were obtained from the direct sequencing of PCR products from each primer pair. Subsequently, a population of RILs contrasting for the presence / absence of seed lipoxygenases, were used for the validation of two SNPs as markers for genes Lox 2 and a indel marker for LOX 3. The genotyping method used was the direct sequencing of fragments that contained the polymorphisms studied. Two SNPs for LOX 2 and the indel contained in the first exon of the gene LOX 3 were co- segregated with the character presence/absence of LOX 2 and 3 respectively in the individuals of the population of RILs. These polymorphisms have the potential to be used as tools in soybean breeding assisted by molecular markers.A degradação oxidativa dos ácidos graxos da fração óleo de soja, que ocorre durante o processamento dos grãos, desenvolve o sabor de feijão cru que altera a palatabilidade e conseqüentemente aceitabilidade dos produtos à base de soja, resultando em problemas para a indústria de alimentos. A enzima lipoxigenase (linoleato: O2 oxirredutase, EC 1.13.11.12), catalizadora dessa reação oxidativa, está presente na semente de soja sob a forma de três isoenzimas codificadas por três genes dominantes, Lox1, Lox2 e Lox3. A seleção assistida por marcadores moleculares é uma área que possui grande impacto dentro dos programas de melhoramento. Recentemente, uma nova classe de marcadores moleculares, denominados indel (Inserção/deleção) e SNP (Single Nucleotide Polymorphism), vem sendo utilizada. Estes se baseiam na detecção de polimorfismos resultantes da alteração de uma única base no genoma. A identificação de marcadores moleculares dentro dos genes que codificam as lipoxigenases poderá auxiliar na seleção de alelos recessivos que determinam a ausência de lipoxigenases na semente, acelerando assim o processo de melhoramento da soja. Os objetivos desse trabalho foram a confirmação e identificação de polimorfismos presentes nas seqüências dos genes que codificam as lipoxigenases 2 e 3, respectivamente; entre variedades, de origem genética distintas, utilizadas no Programa de Melhoramento da Qualidade da Soja-BIOAGRO/UFV e validação de alguns desses polimorfismos como marcadores moleculares. Um par de primers para a LOX2 e 14 pares de primers desenhados para amplificar todo o gene da LOX3, foram utilizados para a obtenção dos fragmentos dos genes. As seqüências foram obtidas a partir do seqüenciamento direto dos produtos de PCR de cada par de primer. Posteriormente, uma população de RILs contrastantes para presença/ausência das lipoxigenases das sementes, foi utilizada para a validação de dois SNPs como marcadores dos genes LOX 2 e um indel como marcador para LOX 3. O método de genotipagem usado foi o seqüenciamento direto dos fragmentos que continham os polimorfismos em estudo. Dois SNPs para LOX 2 e o indel contido no primeiro éxon do gene LOX 3 co-segregaram com o caráter presença/ausência de LOX 2 e 3 respectivamente nos indivíduos da população de RILs. Esses polimorfismos têm potencial para serem utilizados como ferramentas no melhoramento da soja assistido por marcadores moleculares.Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade Federal de ViçosaMestrado em Bioquímica AgrícolaUFVBRBioquímica e Biologia molecular de plantas; Bioquímica e Biologia molecular animalMarcadores molecularesLipoxigenasesSojaMolecular markersLipoxygenasesSoybeanCNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA::MELHORAMENTO VEGETALIdentificação e validação de marcadores moleculares indel e SNP para lipoxigenases de sementes de sojaIdentification and validation of indel and SNP molecular markers for soybean seed lipoxygenasesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFVORIGINALtexto completo.pdfapplication/pdf1599928https://locus.ufv.br//bitstream/123456789/2411/1/texto%20completo.pdf955df50ecdef08e2302f4bed2634d622MD51TEXTtexto completo.pdf.txttexto completo.pdf.txtExtracted texttext/plain141937https://locus.ufv.br//bitstream/123456789/2411/2/texto%20completo.pdf.txtb5647c341cb4288cea26e97dc4c2a632MD52THUMBNAILtexto completo.pdf.jpgtexto completo.pdf.jpgIM Thumbnailimage/jpeg3750https://locus.ufv.br//bitstream/123456789/2411/3/texto%20completo.pdf.jpg4a2df2bd42a77cc50ab7cec9d9b88986MD53123456789/24112016-04-07 23:20:59.882oai:locus.ufv.br:123456789/2411Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452016-04-08T02:20:59LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.por.fl_str_mv |
Identificação e validação de marcadores moleculares indel e SNP para lipoxigenases de sementes de soja |
dc.title.alternative.eng.fl_str_mv |
Identification and validation of indel and SNP molecular markers for soybean seed lipoxygenases |
title |
Identificação e validação de marcadores moleculares indel e SNP para lipoxigenases de sementes de soja |
spellingShingle |
Identificação e validação de marcadores moleculares indel e SNP para lipoxigenases de sementes de soja Silva, Danielle Fernandes da Marcadores moleculares Lipoxigenases Soja Molecular markers Lipoxygenases Soybean CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA::MELHORAMENTO VEGETAL |
title_short |
Identificação e validação de marcadores moleculares indel e SNP para lipoxigenases de sementes de soja |
title_full |
Identificação e validação de marcadores moleculares indel e SNP para lipoxigenases de sementes de soja |
title_fullStr |
Identificação e validação de marcadores moleculares indel e SNP para lipoxigenases de sementes de soja |
title_full_unstemmed |
Identificação e validação de marcadores moleculares indel e SNP para lipoxigenases de sementes de soja |
title_sort |
Identificação e validação de marcadores moleculares indel e SNP para lipoxigenases de sementes de soja |
author |
Silva, Danielle Fernandes da |
author_facet |
Silva, Danielle Fernandes da |
author_role |
author |
dc.contributor.authorLattes.por.fl_str_mv |
http://lattes.cnpq.br/7918289438245309 |
dc.contributor.author.fl_str_mv |
Silva, Danielle Fernandes da |
dc.contributor.advisor-co1.fl_str_mv |
Barros, Everaldo Gonçalves de |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781285J6 |
dc.contributor.advisor-co2.fl_str_mv |
Oliveira, Luiz Orlando de |
dc.contributor.advisor-co2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781626T2 |
dc.contributor.advisor1.fl_str_mv |
Moreira, Maurílio Alves |
dc.contributor.advisor1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4796105P2 |
dc.contributor.referee1.fl_str_mv |
Miranda, Fábio Demolinari de |
dc.contributor.referee1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4706159T4 |
dc.contributor.referee2.fl_str_mv |
Schuster, Ivan |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/9991337319211428 |
dc.contributor.referee3.fl_str_mv |
Moraes, Rita Maria Alves de |
dc.contributor.referee3Lattes.fl_str_mv |
http://lattes.cnpq.br/2396993596781287 |
contributor_str_mv |
Barros, Everaldo Gonçalves de Oliveira, Luiz Orlando de Moreira, Maurílio Alves Miranda, Fábio Demolinari de Schuster, Ivan Moraes, Rita Maria Alves de |
dc.subject.por.fl_str_mv |
Marcadores moleculares Lipoxigenases Soja |
topic |
Marcadores moleculares Lipoxigenases Soja Molecular markers Lipoxygenases Soybean CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA::MELHORAMENTO VEGETAL |
dc.subject.eng.fl_str_mv |
Molecular markers Lipoxygenases Soybean |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA::MELHORAMENTO VEGETAL |
description |
The oxidative degradation of fatty acids from soybean oil fraction, that occurs during the grain processing, develop a flavor of unripe been that modify the palatability and therefore the acceptability of soybean products, resulting in problems for the food industry. The enzyme lipoxygenases (linoleate: O2 oxidoreductase, EC 1.13.11.12), that catalyses this oxidation reaction, are present in soybean seed in the three isozyme forms encoded by three dominant genes, Lox1, Lox2 and Lox3. The selection assisted by molecular markers is an area that has great impact within breeding programs. Recently, a new class of molecular markers, called indel (insertion / deletion) and SNP (Single Nucleotide Polymorphism) have been used. These are based on detection of polymorphisms by the change of a single base in the genome. The identification of molecular markers within the genes encoding lipoxygenase may help in the selection of recessive alleles that determine the absence of lipoxygenase in the seed, accelerating the process of soybean breeding. The objective of this study was verify and identify the polymorphisms in the sequences of genes Lox2 and Lox3, respectively, between varieties of different genetic origin used in the Program of Improvement and quality of Soybean at BIOAGRO/UFV, and tovalidate some of these polymorphisms as molecular markers. A pair of primers for LOX2 and 14 pairs of primers designed to amplify the entire gene LOX3 were used to obtain genes fragments. The sequences were obtained from the direct sequencing of PCR products from each primer pair. Subsequently, a population of RILs contrasting for the presence / absence of seed lipoxygenases, were used for the validation of two SNPs as markers for genes Lox 2 and a indel marker for LOX 3. The genotyping method used was the direct sequencing of fragments that contained the polymorphisms studied. Two SNPs for LOX 2 and the indel contained in the first exon of the gene LOX 3 were co- segregated with the character presence/absence of LOX 2 and 3 respectively in the individuals of the population of RILs. These polymorphisms have the potential to be used as tools in soybean breeding assisted by molecular markers. |
publishDate |
2009 |
dc.date.issued.fl_str_mv |
2009-07-06 |
dc.date.available.fl_str_mv |
2010-02-24 2015-03-26T13:07:27Z |
dc.date.accessioned.fl_str_mv |
2015-03-26T13:07:27Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SILVA, Danielle Fernandes da. Identification and validation of indel and SNP molecular markers for soybean seed lipoxygenases. 2009. 78 f. Dissertação (Mestrado em Bioquímica e Biologia molecular de plantas; Bioquímica e Biologia molecular animal) - Universidade Federal de Viçosa, Viçosa, 2009. |
dc.identifier.uri.fl_str_mv |
http://locus.ufv.br/handle/123456789/2411 |
identifier_str_mv |
SILVA, Danielle Fernandes da. Identification and validation of indel and SNP molecular markers for soybean seed lipoxygenases. 2009. 78 f. Dissertação (Mestrado em Bioquímica e Biologia molecular de plantas; Bioquímica e Biologia molecular animal) - Universidade Federal de Viçosa, Viçosa, 2009. |
url |
http://locus.ufv.br/handle/123456789/2411 |
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por |
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por |
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Universidade Federal de Viçosa |
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Mestrado em Bioquímica Agrícola |
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UFV |
dc.publisher.country.fl_str_mv |
BR |
dc.publisher.department.fl_str_mv |
Bioquímica e Biologia molecular de plantas; Bioquímica e Biologia molecular animal |
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Universidade Federal de Viçosa |
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