Influência do exercício físico regular sobre alguns parâmetros morfológicos renais de ratos com Diabetes mellitus experimental

• Ano de defesa: 2012
• Autor(a) principal: Soares, Pedro Natali Pinheiro
• Orientador(a): Natali, Antônio José
• Banca de defesa: Franco, Frederico Souzalima Caldoncelli
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Viçosa
• Programa de Pós-Graduação: Mestrado em Educação Física
• Departamento: Aspectos sócio-culturais do movimento humano; Aspectos biodinâmicos do movimento humano
• País: BR
• Palavras-chave em Português: Exercício físico; Diabetes mellitus; Nefropatia diabética
• Palavras-chave em Inglês: Exercise; Diabetes mellitus; Diabetic nephropathy
• Área do conhecimento CNPq: CNPQ::CIENCIAS DA SAUDE::EDUCACAO FISICA
• Link de acesso: http://locus.ufv.br/handle/123456789/3470

The aim of this study was to investigate the effects of a swimming training program on the hitophysiology of the kidney in rats with experimental diabetes mellitus (DM). Thirty-days old male Wistar rats (Rattus norvegicus) with mean body mass of 87.42 g were randomly divided into four experimental groups: sedentary control (SC, n=8); exercised control (EC, n=6); sedentary diabetic (SD, n=9); and exercised diabetic (ED, n=13). Animals from ED and SD groups received an intraperitoneal injection (60 mg/kg body mass) of streptozotocin (STZ) diluted in 1 mL of sodium citrate buffer (0.1 M, pH 4.5). Animals from, SC and EC groups received the same dose of sodium citrate buffer (0.1 M, pH 4.5) without STZ. Seven days after STZ injection those animals with fasting glucose above 300 mg\dL were considered diabetic. After 45 days of hyperglycemia (diabetes), animals from ED and EC groups were subjected to a swimming training program for 8 weeks. After euthanasia, the kidneys were removed, weighed and processed for histomorphometric analysis. The outer diameter and the outer and inner areas of the glomeruli were measured. PAS positive cells were counted. EC animals presented the inner and outer areas of renal glomerulus significantly higher than ED animals (P<0,05). The diameter of glomeruli was not different between groups (P>0,05). SC and EC groups did not show accumuation of glycogen in the renal tubules, however the diabetic animals did. The exercise protocol employed was not able to reverse this situation. It was concluded that DM increased the number of renal cells with glycogenic degeneration, but did not alter renal histomorphometry. The exercise protocol used did not change this frame.