Filogeografia molecular e atividade de enzimas digestivas do caruncho-do-feijão Acanthoscelides obtectus (Bruchidae:Coleoptera)

• Ano de defesa: 2008
• Autor(a) principal: Oliveira, Márcia Rodrigues Carvalho
• Orientador(a): Oliveira, Luiz Orlando de
• Banca de defesa: Santos, Jorge Abdala Dergam dos , Caixeta, Eveline Teixeira , Lamêgo, Márcia Rogéria de Almeida
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Viçosa
• Programa de Pós-Graduação: Doutorado em Bioquímica Agrícola
• Departamento: Bioquímica e Biologia molecular de plantas; Bioquímica e Biologia molecular animal
• País: BR
• Palavras-chave em Português: Filogeografia; Enzimas digestivas; Caruncho do feijão
• Palavras-chave em Inglês: Phylogeography; Digestive enzymes; Bean-weevil
• Área do conhecimento CNPq: CNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::BIOLOGIA MOLECULAR
• Link de acesso: http://locus.ufv.br/handle/123456789/292

In the world, Brazil stands out as a major producer and consumer in the world of common bean, Phaseolus vulgaris, however has low productivity due to several factors among them, low technological level used in the cultivation and lack of care mainly plant grain during the storage of . Large losses in the storage of bean (P. vulgaris) are attributed to grain legumes weevil causing the decline of their nutritional value. The main enzymes related to energy and protein metabolism of bruchid are serine and cysteine proteases and α-amylase. The different levels of activities of digestive enzymes suggested a higher metabolic efficiency. Therefore, the process of resistance may be attributed to better adaptation to the natural inhibitors present in the plant leading to reproductive success and perpetuation of the species. Here, we report a phylogeography study that investigated the genetic diversity and an enzyme study that examined the energy metabolism in of Acanthoscelides obtectus. The phylogeography study was based on two genes of mitochondrial genome, COI and rRNA16S, which revealed that populations from either Mexico or Peru consisted of one lineage each. Brazilian populations contained two distinct mitochondrial lineages, both with low genetic diversity, and possibly originated from the Mesoamerican lineage. Probably these lineages came to Brazil only once forming two ancestral lines. Alternatively, they were introduced repeatedly from the same parental source. One of the lines is geographically restricted to South Brazil, whereas the other lineage is widespread all over Brazil. Comparative analysis of the proteolytic and amidolytic activity of cysteine and serineprotease between four study populations (Sobradinho, Viçosa, Cacoal, and Bom Jesus do Amparo) revealed Sobradinho with a higher activity of serine and cysteine proteases. Most likely, Sobradinho exhibited more efficient metabolism in relation to the protease inhibitors present in seeds. The population Viçosa showed higher activity of amylase followed by Bom Jesus do Amparo, while Cacoal and Sobradinho had lower activity of amylase and did not differ statistically.