Glândulas salivares labiais de cães: estudo in situ e após transplantação em olhos hígidos ou portadores de ceratoconjuntivite seca experimental

• Ano de defesa: 2014
• Autor(a) principal: Galvão, Simone Rezende
• Orientador(a): Carlo, Ricardo Junqueira Del
• Banca de defesa: Cunha, Daise Nunes Queiroz da , Filgueiras, Richard da Rocha , Pontes, Kelly Cristine de Sousa
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Viçosa
• Programa de Pós-Graduação: Doutorado em Medicina Veterinária
• Departamento: Biotecnologia, diagnóstico e controle de doenças; Epidemiologia e controle de qualidade de prod. de
• País: BR
• Palavras-chave em Português: Cão - Doenças; Glândulas salivares; Cão; Olhos; Ceratoconjuntivite
• Palavras-chave em Inglês: Dog - Diseases; Salivary glands; Dog; Eyes; Keratoconjunctivitis
• Área do conhecimento CNPq: CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA::CLINICA E CIRURGIA ANIMAL::CLINICA CIRURGICA ANIMAL
• Link de acesso: http://locus.ufv.br/handle/123456789/1472

Labial salivary glands were evaluated in situ and after being transplanted into the upper eyelid conjunctiva of healthy eyes or eyes presenting experimentally induced keratoconjunctivitis sicca (KCS). For in situ study, labial salivary glands together with oral mucosa were removed from 10 cadavers of adult dogs as one piece from the anatomic region of the second pre-molar to the distal border of the second molar of the lower left lip. Samples from the rostral, medial and caudal piece were processed for morphologic, morphometric and histochemical evaluations. For the in vivo study constituted of 2 steps, 10 healthy adult dogs were used, distributed in two groups of five animals each. In the first step, the upper conjunctive eyelid of the left eye of five animals received autogenous labial salivary gland transplant (LSGT), forming the healthy/LSGT group and the contralateral eye was used as control. The host and donor sites were clinically evaluated, the influence of the transplant on the healthy eye with ophthalmic clinical examination and on the 17th week after the transplant, morphologic, morphometric and histochemical aspects of the glandular tissue were analyzed. For step 2, in 5 animals KCS was experimentally induced on the left eye. After the ophthalmic clinical establishment of KCS the upper conjunctive eyelid of the left eye of the animals received autogenous LSGT, forming the KCS/LSGT group. The host and donor sites were evaluated as well as the influence of the autogenous labial salivary glands on KCS by ophthalmic clinical examination, and morphologic, morphometric and histochemical aspects of the glandular tissue 17 weeks after transplant. The 1.2 cm long autogenous LSGT was obtained from the anatomic region of the last lower pre-molar to the first molar of the lower left lip and placed in the upper conjunctive eyelid. In the in situ study, labial salivary glands were easily palpated in the harvest point, becoming better grouped caudally and classified as exocrine, compound, tubuloacinar, mixed, predominantly mucous with semilunar serous parts involving some secretory mucous units, with a duct system formed by striated and intercalated intralobular ducts, interlobular ducts and main excretory duct. In the rostral region, a lower amount of parenchyma existed in relation to the remaining regions and acini diameters remained the same in the three regions. PAS and Alcian blue positive reactions in the three regions were observed, demonstrating the presence of neutral, carboxylated acid and sulphated acid mucins. Serous semilunar parts presented positive reaction to Xylidine Ponceau, indicating the presence of proteins. In the in vivo study, the 1.2 cm autogenous LSGT was collected and placed in the upper conjunctive eyelid without any complications for the donor and host sites, respectively. In healthy eyes after labial gland transplant there was no increase in tear production (p>0.05) nor an unfavorable interference both on the lachrymal film stability and cornea surface. Eyes with experimentally induced KCS associated to transplants presented favorable clinical signs when compared to their clinical presentation after KCS induction, with salivary secretion confirmed by Schirmer I and by histochemical analysis. Salivary secretion lubricated ocular surface making the evaluation of devitalized cells favorable. Labial salivary glands transplanted in healthy eyes maintained better their histological architecture than KCS eyes, while in both the transplants maintained their histochemical patter.