Efeitos do extrato hidroalcoólico da raiz de Pfaffia glomerata (Spreng.) Pedersen sobre testículo e pênis de camundongos adultos

• Ano de defesa: 2012
• Autor(a) principal: Matta, Ana Paula de Lima Florentino
• Orientador(a): Matta, Sérgio Luis Pinto da
• Banca de defesa: Chiarini-garcia, Hélio , Neves, Mariana Machado , Leite, João Paulo Viana , Barros, João Bosco Gonçalves de
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Viçosa
• Programa de Pós-Graduação: Doutorado em Biologia Celular e Estrutural
• Departamento: Análises quantitativas e moleculares do Genoma; Biologia das células e dos tecidos
• País: BR
• Palavras-chave em Português: Pfaffia glomerata; Testículo; Corpos cavernosos; Testosterona; Fitoterapia
• Palavras-chave em Inglês: Pfaffia glomerata; Testis; Cavernosa; Testosterone; Phytotherapy
• Área do conhecimento CNPq: CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
• Link de acesso: http://locus.ufv.br/handle/123456789/270

In several parts of the world is the widespread practice of herbal therapies with aphrodisiac purposes. Pfaffia glomerata is a species commonly used as a sexual stimulant, tranquilizer, anti-inflammatory and healing effects. This work aimed to evaluate the effects of the hydroalcoholic extract of the roots of P. glomerata (HERPG) on penile and testicular parameters and serum testosterone level of adult mice. The extract was subjected to phytochemical prospection. We used 24 animals (6 per group) distributed as follows: group 1 (G1) received water, group 2 (G2) received dimethylsulfoxide (DMSO), group 3 (G3) and 4 (G4) who received 300 and 400mg/kg body weight the HERPG, respectively, diluted in 0.2 ml DMSO. All groups were subjected to mild caloric restriction (10%). Phytochemical analysis revealed the presence of flavonoids, tannins, triterpenes/steroids and saponins. The two treated groups treated with HERPG showed an increase in the proportion the tunica propria, of the lumen and the body weight, and showing reduction in the proportion in volume of the seminiferous epithelium, and the diameter tubular and gonadosomatic (GSI), tubulesomatic (TSI) and Sertoli cell indexes. These animals also showed a high rate of apoptotic germ cells, besides the occurrence of tubular pathology. Group 4 showed also a reduction in height of the seminiferous epithelium, the numbers of primary spermatocytes in preleptóteno/leptotene and at pachytene, in total capacity of Sertoli cell-support and sperm production. Both treated groups showed significant dilatation of the corpus cavernosum, and the concentration of 400mg animals also had increased level of serum testosterone. It can be concluded that the HERPG has adverse effects on spermatogenesis in mice, producing pathological changes in Sertoli and germ cells and inducing apoptosis of germ cells, and reduce sperm production. In addition, provides a possible vasodilator and stimulating the secretion of testosterone.