Morfologia dos espermatozóides do parasitóide Apanteles galleriae Wilkinson, 1932(Hymenoptera: Ichneumonoidea: Braconidae)

• Ano de defesa: 2007
• Autor(a) principal: Coelho, Patrizia Mello
• Orientador(a): Lino Neto, José
• Banca de defesa: Serrão, José Eduardo , Matta, Sérgio Luis Pinto da
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Viçosa
• Programa de Pós-Graduação: Mestrado em Biologia Celular e Estrutural
• Departamento: Análises quantitativas e moleculares do Genoma; Biologia das células e dos tecidos
• País: BR
• Palavras-chave em Português: Vespas parasíticas; Espermatozóide; Morfologia; Apanteles galleriae
• Palavras-chave em Inglês: Vespas parasíticas; Spermatozoa; Morphology; Apanteles galleriae
• Área do conhecimento CNPq: CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
• Link de acesso: http://locus.ufv.br/handle/123456789/2300

Braconidae represents the second largest family of Hymenoptera and, being parasitic wasps of other insects, are potentially important for the biological control. As other families of Hymenoptera, there is still controversy over the systematic of the Braconidae subfamilies, as well as between them and the other families of the Ichneumonidae superfamily. The ultra-structural characterization has contributed to the phylogenetic study of many groups of animals, including insects. Thus, this present work describes the structure and ultra-structure of the parasitoid Apanteles galleriae sperm, using light and transmission electron microscopy. A. galleriae sperms are similar to those described for other non- cyclostomes Braconidae: (1) they are the minors sperm, measuring 12 µm length, (2) axonemes with a 9 + 1 + 1 microtubules configuration, (3) the two mitochondrial derived products do not present cristae and have the same diameter, smaller than that of the axoneme and (4) two accessory bodies with a indefinite format. They also have structural features only common to parasitic wasps, as a thick layer in the plasmatic membrane that covers the acrosome and part of the nucleus and the axoneme microtubules accessories finishing before the others, but no other structure that approaches to the centriolar adjunct was identified. Although, sperms of this species have apomorphic characteristic as (1) the acrosome made only by the acrosomal vesicle and (2) condensed chromatin filaments in the periphery of the nucleus. Therefore, these data confirm that, in Hymenoptera, the sperms provide morphological characters that can be used in phylogenetic analysis at different taxonomic levels.