Controle de biofilmes em membranas de osmose inversa através de bacteriófagos

• Ano de defesa: 2014
• Autor(a) principal: Dias, Roberto Sousa
• Orientador(a): Paula, Sérgio Oliveira de
• Banca de defesa: Ramos, Javier Vega , Silva, Cláudio Mudado , Merzel, Valéria Maia , Silva Júnior, Abelardo
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Viçosa
• Programa de Pós-Graduação: Doutorado em Biologia Celular e Estrutural
• Departamento: Análises quantitativas e moleculares do Genoma; Biologia das células e dos tecidos
• País: BR
• Palavras-chave em Português: Biofilme; Bacteriologia; Bactérias - Morfologia; Arthrobacter; Podoviridae, Osmose; Petróleo - Refinarias
• Palavras-chave em Inglês: Biofilm; Bacteriology; Bacteria - Morphology; Arthrobacter; Podoviridae, Osmosis; Petroleum - Refineries
• Área do conhecimento CNPq: CNPQ::CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
• Link de acesso: http://locus.ufv.br/handle/123456789/281

Some industries are known to produce large amounts of saline effluents, among them we can highlight the petrochemical industry. With the increasing search for technologies that allow recycling of water, reverse osmosis systems (RO) has gained great attention from researchers and industrial sector around the world. Fouling is the main obstacle encountered by these systems, its cause a significant reduction in the flow and may have various origins, being biofouling, characterized by the formation of microbial biofilm on the membrane surface, the more important. This work evaluated the ability of phage vB_AspP-UFV1, specific against bacteria of the genus Arthrobacter, subjected to morphological and molecular analyzes and isolated from activated sludge systems, in control of biofilm formed by bacteria obtained from oil refineries feed water reverse osmosis system. The results show approximately 22 Kb genome Podoviridae family phage, order Caudovirales. The techniques of crystal violet and scanning electron microscopy demonstrated the ability of phage to reduce biofilm formation in most bacteria assessed, achieving 87% reduction for some isolates, which demonstrates the feasibility of using bacteriophages as alternative to current methods of microbiological control in industrial systems. Biofilm formation was assessed on the surface of the membranes in a filtration of industrial wastewater from oil refinery, operated for 30 days in a pilot reverse osmosis system. The presence of thick biofilm after 15 days of operation was observed in addition to the almost complete absence of viable cells on the thirtieth day. Five specific phages against bacteria found in the feed water of reverse osmosis systemwere isolated, thus aiming at the establishment of a phage cocktail containing specific and unspecific phages with potential application in biofouling control.