Equivalência em fósforo disponível da fitase usando diferentes metodologias com frangos de corte e suínos

• Ano de defesa: 2013
• Autor(a) principal: Cruz, Sandra Carolina Salguero
• Orientador(a): Rostagno, Horácio Santiago
• Banca de defesa: Sorbara, José Otávio Berti , Rodrigueiro, Ramalho José Barbosa
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Viçosa
• Programa de Pós-Graduação: Doutorado em Zootecnia
• Departamento: Genética e Melhoramento de Animais Domésticos; Nutrição e Alimentação Animal; Pastagens e Forragicul
• País: BR
• Palavras-chave em Português: Fósforo; Aspergillus oryzae; Frangos de corte; Suínos
• Palavras-chave em Inglês: Phosphorus; Aspergillus oryzae; Broilers; Swine
• Área do conhecimento CNPq: CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA::NUTRICAO E ALIMENTACAO ANIMAL::AVALIACAO DE ALIMENTOS PARA ANIMAIS
• Link de acesso: http://locus.ufv.br/handle/123456789/1837

Two experiments were conducted to determine the phosphorus equivalent of a bacterial phytase expressed in Aspergillus oryzae in the diet Based on corn and soybean meal with different levels of available phosphorus (Pav) on performance, bone characteristics, digestibility apparent and phosphorus balance in broilers and pigs assessed with different methodologies. In the first experiment was used 560 male broiler chicks from 7 days of age housed in metabolism cages were distributed in a completely randomized design, with seven treatments, eight replicates of 10 birds each. A basal diet was used to achieve the recommendations for broiler chicks in the early phase (8- 21 days) except for Pav and total calcium (CaT) (0.17% and 0.9% respectively), using phosphate dicalcium (FB) as a source of inorganic phosphorus. To compose the treatments 2, 3 and 4 were added 0.06, 0.12 and 0.18% of Pav from FB in the treatments 5, 6 and 7 was supplied 500, 1000 and 2000 FYT of a microbial phytase expressed in Aspergillus oryzae. Each experimental diet was added 1% Acid Insoluble Ash (AIA) (Celite). The experimental period was 14 days where were evaluated the weight gain, feed intake and feed conversion. In the period from the 16 to 20 days was collected the total excreta and 21 day all birds were slaughtered for the withdrawal of the tibia and ileal content. The experimental data were subjected to analysis of variance, the averages of the parameters were used to create a standard curve, linear and quadratic equations to know the equivalence of Pav. Weight gain, feed conversion and apparent ileal digestibility had a linear and quadratic effect (P <0.01) when increased the levels of FB Pav and phytase. However was not observed significant achievement in feed intake. The tibia bone weight (g), ash (g%) and phosphorus (P) (mg g) showed a linear effect (P <0.01) with increasing levels of phytase and Pav. However, there was a quadratic effect, P (mg and%) with increasing levels of phytase. The balance of P showed linear effect when increased the levels of phytase. The equivalency values match the standard curve method were: 0.107, 0.131 and 0.155% of Pav by the method of linear equations were: 0.0625, 0.1155 and 0.1686% of Pav and the method of quadratic equations were: 0.0996, 0.1191 and 0.1386% of Pav. The average values of equivalency of phosphorus using the different methods were: 0.090, 0.122 and 0.154% P available for 500, 1000 and 2000 FYT respectively for phytase Ronozyme HiPhos. The second experiment was used 144 barrows with average weight of 18 ± 0.84 kg allocated in a randomized complete block, with 6 treatments and 3 replicates of 8 barrows each. The basal diet (T1) based on corn and soybean meal and low in Pav (0.157%), the T2 and T3 were made up by adding 0.1 and 0.2% of Pav from Dicalcium Phosohate respectively. The T4, T5 and T6 were suppplied 500, 1000 and 2000 FYT phytase expressed in bacterial Aspergillus oryzae (RonozymeHiPhos). Each treatment was used 1% of ash insoluble acidic as faecal indicator. The experiment lasted 21 days and was evaluated weight gain, feed intake and feed conversion. In the period 16-20 day was done the partial collection of faeces to evaluate the apparent digestibility of P, and on day 21 was shot down by an animal cage for obtaining metatarsal and thus assess the deposition of ash (g, %) and phosphorus (mg and %). The experimental data was subjected to analysis of variance, the averages of the parameters were used to obtain a standard curve, and linear equations for calculating the equivalence of Pav. Weight gain and feed conversion increased linearly (P <0.01) with increasing levels of FB from Pav and phytase. However we did not observe significant achievement in feed intake. The characteristics of the metatarsal bone weight (g), ash (g, %) and P (g, mg) showed a linear effect (P <0.01) with increasing levels of Pav. Also a quadratic effect (P <0.01) was showed when the levels of phytase increased the exception of P%. Linear effect was observed (P <0.01) and quadratic (P <0.01) apparent digestibility of P in the supply of Pav and phytase. The equivalency values of phosphorus by the method of the standard curve were 0.066, 0.123 and 0.181% P available for 500, 1000 and 2000 FYT respectively, also for the method of linear equations: 0.060, 0.123 and 0.188% P available for 500, 1000 and 2000 FYT phytase respectively. The average values of equivalency of phosphorus by the method of the standard curve and linear equations are: 0.064, 0.126 and 0.187% of Pav 500, 1000 and 2000 FYT respectively for phytase Ronozyme HiPhos.